Alright, let's solve this problem step by step. We have 53939 and 20385, and we're subtracting the second number from the first.

Step 1: We'll start by subtracting the digit 5 and the borrow 0 from 9 in column 1 and get 4.
4 is the first digit of our result.

Step 2: We'll start by subtracting the digit 8 and the borrow 0 from 3 in column 2 and get -5.
We add -5 to 10 and get 5 as the next digit of the result.

Step 3: We'll start by subtracting the digit 3 and the borrow 1 from 9 in column 3 and get 5.
5 is the next digit of our result.

Step 4: We'll start by subtracting the digit 0 and the borrow 0 from 3 in column 4 and get 3.
3 is the next digit of our result.

Step 5: We'll start by subtracting the digit 2 and the borrow 0 from 5 in column 5 and get 3.
3 is the next digit of our result.

So, 53939 - 20385 = 33554.