a, CD loop folding in hydrophobic buffer. Peptides were synthesized that correspond to the predicted amphipathic helix from S. cerevisiae Atg18 (TRLAREPYVDASRKTMGRMIRYSSQ) or to a sequence with reduced tendency to form amphipathic helices, generated by a swap of two amino acid pairs (indicated by arrows; SLoop). Helical wheel projections of the sequences are shown. 