E. The abundance of all detected polar metabolites was compared between iRBCs and uRBCs and presented as the log2 fold-change (iRBC/uRBC) with respect to the -log10P. Significance cut-off was set with −log10(0.05) + c/(x − x0). Each data point represents a single metabolite and the insets depict the extracted ion chromatograms for two metabolites that were either elevated (acetyl-CoA) or decreased (fructose-1,6-bisphosphate) in iRBC relative to uRBC. 