A) Concentration of protein remaining in the supernatant after centrifugation, which is an inverse measure of phase separation, changes with salt concentration. hnRNPA2 LC shows low phase separation (high protein remaining in the supernatant) at low salt conditions and near complete phase separation (no protein remaining in the supernatant) at high salt concentrations. In contrast, tyrosine phosphorylated hnRNPA2 LC shows higher phase separation (less protein remaining in the supernatant) at low salt concentrations than at high salt concentrations. Conditions: 20 µM protein, 20 mM MES pH 5.5 150 mM urea, salt concentration as indicated, 25˚C. Error bars are standard deviation of three replicates. 