D. Violin plots of fractional distance analysis showing the average distribution of endosomes in cells from panel (E) and fig. EV3A. Graph represents mean ±s.d. of n=15 cells per condition from 3 independent experiments. Dashed lines represent median and solid lines represent interquartile range. Cartoons representing the overexpression and control LE/Ly phenotype. Significance two-tailed student t-test. **** P<0.0001 