(D) Endogenous Co-IP of BET1 and ERGIC-53. Immunoblots of HEK 293T cell lysates (+ lysate) or lysis buffer (-lysate) incubated with (+) or without (-) BET-1 antibody followed by precipitation with protein A/G beads. ERGIC-53 signal was only present in samples with lysate and antibody. whereas * denotes unspecific signals from beads used for precipitation. As a positive control GOSR2 antibody was used. 