F. The decrease in MCH mRNA levels observed 18h after icv LPS injection is partly abolished by the CCR2 antagonist INCB3344 (10μM) and in CCR2 KO mice, as shown by real-time PCR analysis for MCH in the hypothalamus. Results were normalized to values in icv saline injected WT mice (n=6 per group). Data are expressed as means ± SEM. ** or $$ p<0.01. * saline vs icv LPS injection in WT mice. $ icv LPS injection in WT mice vs LPS+INCB3344 icv injection in WT mice or icv LPS injection in CCR2 KO mice. 