D The expression of Ifnb1 in WT (n=3) and Akt2 KO (n=3) primary-MEFs with VSV treatment (MOI, 0.1) for 6 hours was measured by qRT-PCR (left panel). After VSV treatment for 3 hours, the culture suspension was discarded and wash 3 times with PBS, then primary-MEFs was cultured with fresh medium for another 15 hours for analysis of the VSV-infected (GFP) primary-MEFs by fluorescence microscope (middle panel, representative images) and by FACS assay (right panel). Bar, 100 μm. Data information: *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001 and ns, not significant (P > 0.05); using two-way ANOVA test Data are from three independent experiments or representative of three independent biological replicates Error bars mean ± SEM 