(e) Schematic showing position of Cdc45-interacting region in Sld3. Alignments were generated in Jalview using Clustal. Sld3 from various fungal species is included (S.c., Saccharomyces cerevisiae, S.m., Saccharomyces mikatae, S.b., Saccharomyces bayanus, S.ca., Saccharomyces castellii, S.ku., Saccharomyces kudriavzevii). Residue numbers correspond to S. cerevisiae Sld3. Residues were substituted for Glu. as indicated. 