B The mRNA expression of Akt2 in the brain homogenates of WT mice with JEV injection (5.0×106 PFU/g, i.v.) or in the lung of WT mice with H7N9 (105.5 EID50, i.n.) and PR8 infection (10 LD50, i.n.) were detected by quantitative reverse transcription-PCR (qRT-PCR). Mock, n=4; JEV, n=12; PBS, n=6; H7N9, n=4; PR8, n=8. Data information: *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001 and ns, not significant (P > 0.05); using unpaired t-test or one-way ANOVA test Error bars mean ± SD 