B. Representative time-lapse spinning disk confocal images of n = 3 of live COS7 cells stably expressing mCherry-KDEL (green), transiently expressing EB3-GFP (yellow) and SiR-lysosome-stained LE/Lys (magenta). Zoom insets show regions of interest containing a mobile LE/Lys as denoted by the white arrow. First row shows ER (mCherry-KDEL) (green), the second row shows SiR-lysosome-stained LE/Lys (magenta), the third row shows EB3-GFP (yellow) and the fourth row shows the merge of the three preceding channels. Scale bar: 30µm and zoom insets 3µm. All images acquired for 90 frames at 1Hz. Images related to movie EV6. 