(E, F) Mass spectrometry quantification of m5C (D) and hm5Cm (E) levels in total RNA from nsun mutants. RNA was extracted from populations of L1 animals synchronised by starvation, digested to nucleosides and analysed via LC-MS. n = 3 independent biological replicates. n.d. = not detected. Data information: In E, F data are presented as mean ± SEM. 