(A) HeLa cells were transfected with EGFP-Nup50 and different truncations. After 24h cells were shortly pre-treated with 0.1% Triton X-100 in PBS, fixed with 4% PFA and analyzed by confocal microscopy (left panel). Scale Bar: 10 µm. The graph (right panel) shows the Nup50 domains (blue: Importin α interaction domain, green: NPC targeting domain and pink: Ran binding domain) and the truncation tested. Ability to bind the nuclear rim is indicated on the right. 