WT and Mfn2 KO cells were transfected with plasmids encoding mt-RFP and ChiMERA or control plasmid, and after 48 h they were fixed. N) Representative images. Scale bar= 5 μm. (P) aspect ratio and (Q) circularity were calculated (n= 300 mitochondria analyzed in 3 independent experiments). Data are presented as mean ± SEM. 