(C) The effect of OPA1 overexpression on the number of AP‐containing mitochondria. Imaging of INS1 cells already expressing LC3:GFP and mtDsRed was performed 48-72 h after adenoviral transduction with either OPA1 overexpression or control (mitoPA‐GFP). The number of AP‐containing mitochondria in the OPA1 overexpression (OE, n=6) was normalized to the control (*P=0.001). Images show representative cells from each group. Red, mtDsRed; green, LC3:GFP. In the control cell, note the colocalization of APs with mitochondria. Scale bar, 10 μm. 