G. Schematic of TIRF microscopy-based MT stabilization assay reported in H and I. Various concentrations of unlabelled DCX variants were added to 9 μM unlabelled tubulin mixed with 1 μM HiLyte Fluor™ 488-labeled tubulin in the presence of surface-immobilised HiLyte Fluor™ 488-labeled GMPCPP MT seeds; e-field, evanescent field. 