(A) ssDNA repair efficiency of the rev1∆ mutant treated with MMS only in G1 as determined from the maximal and final percentages of cells with Rad52 foci during the time course (right panel). Cells were synchronized in G1 and treated with 0.033% MMS for 1 hour; after that the MMS was inactivated with 2.5% sodium thiosulfate and cells were released into fresh medium in the absence of DNA damage. The percentage of cells with foci at each point was normalized to the highest value of the wild type, taken as 100 (middle panel). A scheme on the left shows the experimental set up followed in this (MMS in G1 only) and the other experiments (MMS in S/G2). An asterisk represents a blocking DNA lesion for the replicative polymerase. Data information: The mean and SEM of 4 independent experiments are shown. Statistically significant differences according to an unpaired two-tailed Student's t-test are shown, where one, two and three asterisks represent p-values <0.05, <0.01 and <0.001, respectively. 