(D) Details of 2D 15N/1H HSQC spectra of free (green) and Mn-titrated YqgPNTD (10 μM MnCl2 in red and 40 μM MnCl2 in blue) for the residues marked in orange in panel (C). As a reference, residues marked in black do not display spectral changes upon titration, e.g. peak intensity does not change for S88N-H upon Mn titration (overlay of green, blue and red contours), but peak intensity is severely depleted for D29N-H upon Mn titration (absence of red contours). Full titration data are available in Dataset S3. 