MA plot of differential piRNA expression analysis between Siwi-D670A and Siwi overexpressed libraries. Each dot represents the mapped piRNA (> 25-nt) on a predicted silkworm gene. Predicted CDSs with mean RPM > 2 between two independent naive libraries were categorized into non-TE genes (red), potential-TE genes (orange) and TEs (blue) (see also Figure EV4A and Materials and Methods). Siwi-D670A overexpression up-regulates non-TE gene-derived piRNAs while TE-derived piRNAs are not affected. See also Figures EV4 and EV5. 