(B) Immunoprecipitation of exogenous expressed Hsc70 and Htt-552-18Q. Hella cells were transfected with wt and mutant Htt-18Q for 48 h. The lysates were subjected to immunoprecipitation with anti-Hsc70 antibody or no primary antibody. Mutation of Htt-552-18Q at 99-103 was labeled with ▴ and mutation of Htt-552-18Q at 248-252 was labeled with ▪. Ab: Antibody. 