C. Synchronized PfCDPK7-KO or 3D7 ring/trophozoite stage parasites were labelled with BODIPY-ceramide and DAPI followed by live imaging as described for panel A. While tubular vesicular networks/structures (TVNs), vesicles were predominantly labeled in 3D7 parasites, most PfCDPK7-KO parasites did not exhibit TVN; instead, small punctate spots (white arrowheads) were seen in the iRBC cytoplasm (Scale bar-1 μm). 