(C) Quantification of colocalization between mCherry-BspF and GFP-TGN38 in untreated (-CytoD) and Cytochalasin D-treated (+CytoD) HeLa cells. Regions of Interests (ROI, representative shown as insets in panels B) were randomly selected and a Pearson's correlation coefficient was calculated using NIH Fiji image analysis software and Coloc_2 plug-in. Data are means ± SD from n=3 independent experiments in which 2 ROIs from 10 cells (n=20) were analyzed per experiment. The asterisk indicates a statistically significant difference between treatments as determined by a Mann-Whitney test (P<0.05). 