F. The decrease in MCH mRNA levels observed 18h after icv CCL2 injection is abolished by the CCR2 antagonist INCB3344 (10μM) and in CCR2 KO mice, as shown by real-time PCR analysis for MCH in the hypothalamus. Results were normalized to values in icv saline injected WT mice (n=6 per group). Data are expressed as means ± SEM. ** or $$ p<0.01. * saline vs icv CCL2 injection in WT mice. $ icv CCL2 injection in WT mice vs CCL2+INCB3344 icv injection in WT mice or icv CCL2 injection in CCR2 KO mice. 