(A) Representative confocal micrograph of HeLa cells transfected to produce either mCherry (red), GFP-ACAP1 (green) and Arf6-HA (blue; left hand panels) or mCherry-BspF (red), GFP-ACAP1 (green) and HA-Arf6 (blue; right hand panels) and treated with Cytochalasin D (200 nM) for 30 min prior to fixation. Scale bars: 10 µm and 2 µm (insets). 