D) Planar division orientation in the follicular epithelium is independent of Mud. Division angles were calculated relative to the elongating axis of the egg chamber using an unbiased analysis tool. Number of cells analyzed: w1118 n=52; mud3/mud4 n=43; Random number generator n=50; Mean division angle w1118= 31.6o, mud3/mud4= 30.9o, Random number generator = 46o; Wilcoxon Signed Rank Test, Theoretical Mean of 45, w1118 p < 0.0001, mud3/mud4 p = 0.0003, Random number generator p = 0.7779. Spindle angles in mud3/mud4 egg chambers are not significantly different from w1118 (p = 0.7859, Mann Whitney test). Spindle angles in w1118 and mud3/mud4 egg chambers are statistically different from the randomly generated list (p = 0.006 and p = 0.0044, Mann Whitney test) 