(I) Plasmids encoding wild-type GSDMD, GSDMDsusti. or mCherry-GSDMD-C were co-transfected with nucleocapsid into HEK293T cells, followed by immunoprecipitation with antibody against FLAG. Input and precipitates were immunoblotted with antibodies against the indicated protein tags. Data information: Experiments were repeated for three times with similar results. 