(F) Xenopus egg extract were supplemented with 5 µM RanQ69L, RCC1, Xenopus Nup50 wild-type and RCC1 binding mutants as well as a combination of 5 µM Nup50 and 5 µM RCC1. After 90 min, annulate lamellae were isolated by centrifugation and quantified by western blotting with mAB414 antibody. Quantitation shows the relative Nup62 signal as a mean from three independent experiments, normalized to the buffer control. Individual data points are indicated. 