WT and Mfn2 KO cells were transfected with plasmids encoding: B, C) GFP and ChiMERA or control (CT). After 24 h, the transfected cells were sorted and plated for another 24 h, after which were processed for EM. B) Quantification of the percentage of mitochondria in contact with the ER distributed at the indicated distance range (n= 3 independent experiments in which were analyzed 598-779 mitochondria from 68-98 cells). *p<0.05, one-way ANOVA followed by Tukey's post hoc test. C) Average of ER-mitochondria distance (n= 111-193 mitochondria). Data are presented as mean ± SEM. *p<0.05, Kruskal-Wallis test followed by Dunn's post hoc test. Data information: *p<0.05, one-way ANOVA followed by Tukey's post hoc test. Note that experiments were performed at the same time, so the values of WT and Mfn2 KO transfected with control plasmid are the same but the figures have been split in two for the sake of linearity. 