Biochemical strategy employed to trap a post-translocated state of IAPV-IRES in the ribosome. General view of the IAPV-IRES in a post-translocated state on the ribosome: 60S depicted as blue ribbons, 40S as yellow ribbons, IAPV-IRES represented as solid Van Der Waals surface colored according to secondary structure elements described in figure 1A and eRF1* depicted orange. Close up view of the SL-III inserted in the experimental unsharpened cryo-EM density. On the right, refined model with residues from the 60S (blue) in interacting distance with the SL-III (green) indicated. Comparison of the final refined model for IAPV-IRES colored according to the secondary structure described in figure 1A with canonical tRNAs (PDBID: 4V5D) in the pre-translocated state (left) and after translocation (right). Left, overall top view of the inter-subunit space of the 40S for the post-translocated state. Inset, close up view of the 40S tRNA binding sites where it can be appreciated the insertion of PKI of the IAPV-IRES in the P site, projecting the VLR towards the E site. The elements of the 18S rRNA forming the "P site gate" are indicated by solid arrows. On the right, equivalent view for canonical tRNAs. 