A) Schematic of the ub-HS-motif mapping strategy. A 1038 bp stretch of ub-HS4 (blue line) was cloned and integrated at the LEU2 locus (grey) using the integrative YI128-vector (green). Initial mapping led to the identification of fragment F1 (Appendix Fig. S2A), which was further truncated for fine-mapping ((B), F5-F8). qPCR primers were designed to bind within the YI128 backbone. 