(J) Volcano plot showing the altered transcripts induced by TMEM33 OE (clone #1) (p value <0.05) and PKM2 KO (p value <0.05) in MDA-MB-231 cells. Green depicts shared down- regulated genes by both TMEM33 OE and PKM2 KO; red depicts shared up-regulated genes by both TMEM33 OE and PKM2 KO. Variance analysis (ANOVA) was used to calculate the p-value. 