A) Boxplots showing GK effect - the difference between TANGO score and PureTANGO score - per charged GK, and per position with reference to the APR. Negative numbers indicate N-terminal positions, positive numbers refer to C-terminal positions. The upper whiskers indicate the largest value no further than 1.5 times the Inter-Quartile Range (IQR) from the upper hinge, the lower whiskers show the smallest value at least 1.5 * IQR from the lower hinge. Notches extend 1.58 * IQR / sqrt(n) from either side of the median and represent a 95% confidence interval for the median value. Significance was determined through Kruskal-Wallis test with a post-hoc Wilcoxon analysis for pairwise testing and Bonferroni correction for multiple testing. Adjusted p-values are indicated for each comparison. The number of observations per group is indicated beneath each box. 