(A) The effects of knockdown and overexpression of LAMP-2A and Hsc70 on endogenous Htt and p62. PC12 cells were transiently transfected with LAMP-2A, Hsc70 plasmid, vector control or LAMP-2A and Hsc70 siRNA for 48 h. Lysates were subjected to Western blot analysis with antibodies against Htt and p62. Values are the mean±SE of three independent experiments. **P<0.01 (compared with endogenous Htt without treatment). 