(D-E) qRT-PCR measurement of Ifnb1 transcripts in mouse peritoneal macrophages that were left unstimulated or stimulated with 2'3' cGAMP (0.1 μg/mL) (D) or ISD (1 μg/ml) (E) for 4 h in the presence of DMSO or indicated compounds (10 μM). The data are presented as the relative fold induction of Ifnb1/Gapdh transcripts. A 90% reduction of IFNB1 transcripts in mouse peritoneal macrophages in comparison to the DMSO group was selected as the cutoff value for follow-up study, which has been indicated by the red dashed line. The arrow indicates the group that meets the cutoff value. The data shown are mean ± SEM from n=3 biological replicates. 