Left: Inhibitory GlyRs (dark blue), GABAARs (light blue), and gephyrin scaffolds (red) are organized in sub-synaptic domains (SSD) and are aligned with presynaptic release sites (green), forming trans-synaptic nanocolumns (dotted boxes). Right: Increased network activity differentially affects the numbers of GABAAR complexed (light blue pentagons) and the phosphorylation status of gephyrin (phospho-S270 domain, dark red), while GlyRs and total gephyrin levels are largely unaffected. 