At steady state, nuage and piP-body accommodate piRNA factors both stably localized at the condensate or transiently docked to it. Siwi, while does not accumulate in piP-body, transiently enters piP-body and interacts with BmSpnE and BmQin. An active slicer is required for Siwi to segregate from piP-body and go back to nuage, where BmVasa uses its ATPase to remodel RNP interaction and modulates the ping-pong cycle. Some Siwi-interacting factors like BmMael and BmArmi can enter multiple compartments where Siwi is present. When Siwi-D670A is expressed, the slicer mutant is expelled from nuage by RNP remodeling activity of BmVasa and is trapped in piP-body while co-aggregating with piP-body factors, as well as those freely shuttling between compartments. A hypothetic fidelity determining factor could be similarly trapped in piP-body by the mutant, resulting in the up-regulation of non-TE piRNAs. This production could be done in nuage, where native piRNA biosynthesis machineries may still function. Of note, the length distribution and the presence of 2′-O-methyl modification in non-TE piRNAs suggest that these piRNAs are likely to be processed on the outer mitochondrial membrane and in the cytoplasm. It is also possible that non-TE piRNA is directly generated from piP-body, where a battery of piRNA biosynthesis factors abnormally accumulated. 