Pom1 in vitro kinase assays with [ϒ-32P] ATP at five distinct Pom1 concentrations, ranging from 2.6 to 32ng/µl (1x to 12x). Loading of equivalent Pom1 amounts (40ng; see silver staining control) reveals higher incorporation of 32P upon higher Pom1 concentration, indicating an intermolecular reaction. Quantification is shown on the graph. 