A. Domains AAA5s to AAA2L of wild-type and mutant 5 are shown for the ADP-vi state (color) and the AMPPNP state (black). Box: Visualization of inter alpha carbon distances between AMPPNP and ADP-vi state of mutant 5 and wild-type dynein. Black arrows indicate direction of movement when transitioning from the AMPPNP to the ADP-vi state while the size of the arrow indicates the magnitude of movement. All structures are alignment on AAA1L. For wild-type, the structures of human cytoplasmic dynein 2 (ADP-vi state - PDB: 4RH7 and the yeast cytoplasmic dynein (AMPPNP state - PDB: 4W8F were used. For mutant 5 AMPPNP, we used the class 1 structure. B. Same as in (A) but for domains AAA2s to AAA5L. 