F) H3K9me3 redistribution during differentiation. H3K9me3 signal dispersion at the nucleus periphery is low in CD38- (n=137, 3 donors) indicating that H3K9me3 is homogenously distributed at the nucleus periphery. Upon differentiation, H3K9me3 signal dispersion remains homogenous after 24 hours in culture (n=115, 3 donors), but progressively becomes heterogeneous (48 h: n=139, 3 donors) to attain a level at 72 h (n= 148, 3 donors) similar to that in freshly collected CD33+ cells (n=124, 3 donors), indicative of H3K9me3 redistribution from the nucleus periphery. Bars correspond to mean ± SD; ***:p<0.001; **:p<0.01; n.s: non significant; Mann Whitney test. 