Mice were intranasally given 1011 vg of AAV-vector in 50 µl volume three times in three-week intervals. 20 µg of Cal/7/9 WIV per 50 µl was given i.n. two times. Blood samples were taken at indicated time points (red drops). After influenza challenge, mice were monitored for survival and weight loss for two weeks before necropsy. 