Localization of AcGFP- and mCherry-BmVasa-E339Q with wild-type BmVasa as the nuage marker. Z-projections (Maximum intensity) show that while wild-type BmVasa localizes in perinuclear region, BmVasa-E339Q does not necessarily colocalize with its wild-type counterpart but mostly mis-localizes to distal cytoplasmic granules. Scale bar 8 μm. 