B. cdc20ts-mAR PDMC1-AMA1 PHSL1-CDH1 cells were released from the metaphase I-arrest with CuSO4 (t = 0) at 25°C. Subsequent incubation at 25°C allows progression through meiosis II (left), while a shift to 37°C at t = 50 min causes arrest at metaphase II (right). Mpc70-GFP and the PSM marker RFP-Spo2051-91 were imaged in cells fixed at the indicated times. RFP-Spo2051-91 is expressed from an episomal plasmid (Diamond et al., 2009). Images represent ≥ 90% of cells harboring the plasmid. Scale bar, 2 μm. Data information: data are representative of two independent experiments. 