E, Cryo-EM map of the partial ColE9 translocon with an average resolution of 3.7 Å, which has density consistent with OmpF, TolB, and ColE9 residues 3-314. Map is coloured based on component parts shown in panel b. TolB is much better resolved here than in the full translocon map in panel c, although ColE9 density is weaker. ColE9 and TolB density aligns on the extracellular and periplasmic side of OmpF, respectively. 