(A) Redistribution of CMA-active lysosomes induced by overexpression of LAMP-2A. PC12 cells were transfected with PCDNA4-LAMP-2A for72 h. Lysosomes were labeled with immunofluorescence of Cathepsin D (green) and the increased perineuclear localization of CMA-active lysosomes was observed. N: nucleus. Thin arrows point to lysosomes. The scale bar = 10 µm. 