A. Imaging of SPBs (Cnm67-RFP) and Spc72-GFP in PHSL1-CDC20 ama1∆ spo13∆ control cells and cells containing cdc5-as or ime2-as treated with CMK or 1Na-PP1, respectively, at metaphase I (7 h in SPM). Top, time-lapse series. Bottom, Spc72's presence at SPBs was quantified in cells synchronized in silico to SPB separation at entry into metaphase I (t = 0). Data information: data are representative of two independent experiments. 