(B) Hsp70 had no effect on Htt-552 levels. HeLa cells were transiently transfected with Hsp70 plasmid, vector control or Hsp70 siRNA for 72 h and Htt-552 for 48 h. Lysates were subjected to Western blot analysis with anti-Htt 2166 and anti-Hsp70 antibodies. Values are the mean±SE of three independent experiments. 