E. Area under the curve calculated from individual cells as measured in (D). Bars indicate mean±s.e.m. Data information: *p<0.05, **p<0.01, ***p<0.001 , Kruskal-Wallis Anova with Dunn's multiple comparison test Data (total number of cells, n) were obtained from at least three independent transfections with several technical replicates each. 