Workflow of the assay used to evaluate FA transfer by 3T3-F442A adipocyte EV (3T3-EV) to melanoma cells. Mature 3T3-F442A adipocytes were loaded with BODIPY FL C16. Cells were then washed and fresh medium was added. 72h later, conditioned medium was harvested, and 3T3-F442A EV (3T3-EV) were isolated and added to melanoma cells. 