(B) Plasmids encoding FLAG-tagged full-length GSDMD, GSDMD C-terminus and Myc-tagged nucleocapsid were transfected into HEK293T cells individually. Exogenous proteins were purified using FLAG or Myc targeting antibodies coupled to magnetic beads 24 h post transfection. Purified proteins were then mixed and incubated for 2h, followed by immunoprecipitation with antibody against FLAG or isotype IgG. Input and precipitates were immunoblotted as indicated. 