(L) Quantification of overlap between old and new histones in late prophase and prometaphase GSCs and CCs using Spearman correlation: The measurement is from a single Z-slice at the center of each mitotic nucleus, which shows results similar to analyzing every Z-slice throughout the entire Z-stacks followed by averaging them (Fig EV2A, see Materials and Methods). Data was collected over 20, 16, 11, and 12 separate experiments for H3, H4, H2A, and H3Rev, respectively. Values are mean + 95% CI. P-value: pairwise ANOVA test with bonferroni correction. ****: P< 0.0001, n.s.: not significant. 