B Immunoblot analysis of in vitro kinase assay by the phosphorylation-gel. HA-AKT2 was immunoprecipitated from HEK293T cells by anti-HA/IgG antibody and Protein-G beads, and His-IRF3 was purified from E.coli cells by anti-His agarose beads. His-IRF3 alone and anti-IgG-beads added with His-IRF3 served as control. The arrow indicated the phosphorylated IRF3. Data information: * Data are representative of three independent experiments 