Isothermal Titration Calorimetry (ITC) assay of Mac2 binding Paip1M. The raw calorimetric curve is displayed on top while the fitted binding isotherm curve is at the bottom. The measured values are: N = 0.94 ± 0.01 (binding stoichiometry); Kd = 15.5 ± 1.5 μM (dissociation constant); ΔH = -6778 ± 149 cal/mol (standard molar enthalpy change for binding); ΔS = -0.716 cal/mol/K (standard molar entropy change). 