I. Quantification of number of invaded MCF7 cells in 3D matrigel, after incubation with EVs from MDA-MB-231 cells sorted for Greenhigh expression. MDA-MB-231 cells stably expressing a pQXCIP‑zsGreen or pQXCIP‑zsGreen-RASSF1C construct were sorted and EVs were harvested 24 h later, prior to seeding with MCF7 cells in Boyden chambers. Invasive potential of MCF7 cells was evaluated 24 h after incubation with EVs. Data Information: All data are from n=3 independent experiments. Data are analyzed by Student T-test and represented as mean ± SEM.* p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001. 