(C) Identification of highly variable genes in the twitlow cluster using the method by (Brennecke et al, 2013). Scatter plot depicts the mean expression for each gene and squared coefficient of variation across twitlow cells. The solid line indicates the fit, dashed lines the 95% confidence interval. Genes with a significantly elevated variance are shown as triangles, other genes as circles. Different gene classes are color coded. p-values shown were retrieved by a hypergeometric test for enrichment of the respective gene class among highly variable genes. 