(D) T-distributed Stochastic Neighbor Embedding (t-SNE) plot showing the 4 drug tolerant states (NCSC (neural crest stem cells), invasive, SMC (starved-like melanoma cells) and pigmented cells) according to single cell RNA-seq (scRNA-Seq) performed in PDX MEL006 model exposed to BRAFi+MEKi Our BRAFi-resistance genes are mainly expressed in NCSC and invasive cells. (E) AUCell score for each drug tolerant states. 