B-E. Study of gene and protein hypothalamic expression of MCH peptide (D, E) in icv LPS injected mice. Measurement of MCH (E) concentration by EIA after icv LPS injection (black bars) or icv saline injection (grey bars) in mice at 1h, 6h and 18h after injection. Cerebellum was used as negative control (3 independent experiments, n=6 per group in each experiment). 