(a) Top: Schematic representation of the IgH locus depicting Sμ and Sα region with the position of guide RNAs (gRNA) that were used to induce blunt end DSBs using WT SpCas9. Bottom: IgH locus depicting Sμ and Sα region with the position of guide RNAs (gRNA) that were used to induce staggered DSBs with 5′ overhangs using D10N mutants of SpCas9 (Cas9n). 