(A) Yeast two-hybrid mating screen showing interaction of BspF with a fragment of ACAP1 (amino-acid residues 460-740; ACAP1Lib) or full-length ACAP1 (ACAP1FL), compared to positive (p53/T antigen) and negative (empty vectors) control matings plated on permissive double dropout (DDO) or selective quadruple dropout (QDO) media. ACAP1 schematic indicates the region of interaction initially identified (ACAP1Lib). 