E. Expression of the BES1-repressed BR biosynthetic genes ROT3, DWF4 and BR6ox2 (the basic regulatory pathway is illustrated) after a heat shock of 45°C. Plants were treated as in C, samples were harvested and qPCR analyses were performed. Data show the mean ±SD. n=4 biological repeats, each measured in 3 technical replicates, normalized to UBC. The asterisks indicate significant differences compared to the control condition at 21 °C by Student's t-test (*P≤0.05; **P≤0.01; ***P≤0.001). 