Schematic of T-blast cell transformation. Purified CD4 T cells were activated with ConA for 40 hrs and maintained in IL-2 or IL-7 for 30 hours as TB. Cells were cultured for a further 16 hours +/- IL-2, IL-7 or Ruxolitinib. Cells were re-stimulated (TB+) with 20 ng/ml PMA and 2 mM calcium Ionophore (PMA/I) for 2 hours. 