(F) Correct MHE innervation is required for hatching of Drosophila embryos from the eggshell (Friedrich et al., 2016). Hatching rate was calculated based on the number of L1 larvae observed after 24h in genetic crosses, depleted of Dfd (UAS-DfdRNAi) in neurons (elav-GAL4; n=217) compared to crosses with control animals (mock = elav>RFP; n=156). P-values were calculated by a two-sided Fisher test, ***: p < 0.005. 