Heatmap of colocalization ratio between fluorescence protein-tagged piRNA factors. Each colocalization ratio is quantified by using ImageJ plugin Comdet (see Materials and Methods) and averaged from n=3 independent z-stacks. Darker color represents more frequent colocalization. Known nuage proteins (BmVasa, BmAgo3, Siwi) form a cluster at the top-left corner. Siwi-D670A mutant, but not BmAgo3-D697A, clusters with BmSpnE, BmQin and BmDcp2 at the bottom-right corner, representing piP-bodies. See also Figures EV1 and EV2. 