Adeno-associated virus (AAV) construct designed for expression of eGFP and human P301Ltau as individual proteins, separated by the self-cleaving 2a peptide, under the CBA promotor (AAV8 CBA-eGFP-2a-huTauP301L). AAV transduced "donor neurons" express eGFP and huTauP301L, tau "recipient neurons" are identified after immunostaining for human tau as huTau+ but GFP- neurons. 