B) Planar division orientation in the follicular epithelium is dependent on apical tension in a Canoe-dependent manner, with Canoe-shRNA treated egg chambers showing a randomization of division orientation. Division angles were calculated relative to the elongating axis of the egg chamber using an unbiased analysis tool. Number of cells analyzed: w1118 n=52; Canoe-shRNA n=58; Random Number Generator n = 50. Mean division angle w1118= 31.6o, Canoe-shRNA= 43.3o, Random number generator = 46o; Mann Whitney test versus w1118: Canoe-shRNA p = 0.0345 ; Wilcoxon Signed Rank Test, Theoretical Mean of 45, w1118 p < 0.0001, Canoe-shRNA p = 0.7411, Random number generator p = 0.7779. Bars represent mean and standard deviation 