(F) Schemes for GSDMD linker substitution and GSDMD chimera. GSDMD linker spanning amino acids 265-284 were replaced by a mCherry sequence except for the amino acid D275 (GSDMDsusti., upper panel). mCherry-GSDMD-C chimera was generated by displacing GSDMD N-terminus with mCherry (lower panel). The linker was also changed as above. 