A. CRISPR-Cas9 strategy to generate the Tg(kdrl:EGFP) rab13+/∆3'UTR zebrafish strain. The wild type (Wt) rab13 exon 8 is represented with its coding sequence in dark and the 3'UTR in clear boxes; the 5' and 3' gRNA-targeted regions are represented with green lines. Arrows: relative positions of the forward (f) and reverse (r) PCR primers used to identify animals with CRISPR-Cas9-mediated deletions (∆) in the rab13 3'UTR. 