(E) Redistribution of CMA-active lysosomes induced by overexpression of Hsc70. PC12 cells were transfected with Hsc70 plasmid for 48 h. Lysosomes were labeled with immunofluorescence for Cathepsin D (green) in cultured PC12 cells and the re-location of CMA-active lysosomes toward the perinuclear regions was observed. N: nucleus. Thin arrows point to lysosomes. The scale bar represents 10 µm 