Quantification of data shown in panel B. The graphs on the left show the log-2 ratio of Pom1as1-tdTomato fluorescence levels at cell tip and cell middle relative to the medial Pom1-GFP (top) or Pom1KD-GFP (bottom) signal. Note that the of Pom1as1-tdTomato tip/middle log-2 ratios are lowered by 3MB-PP1 addition (red dots) relative to the non-treated samples (blue dots) in the cells co-expressing Pom1KD-GFP, but not in the cells co-expressing Pom1-GFP. A boxplot (right) of the residuals after correction of the effect of the GFP signal illustrates the significant effect of the drug treatment on Pom1as1-tdTomato localization in cells co-expressing Pom1KD-GFP (*, n=30+23, one-sided t-test, p-value =0.012) but not in cells expressing Pom1-GFP (n=30+30, two-sided t-test, p-value=0.39). + and - indicate presence or absence of 3MB-PP1. 