(E) A cartoon depicting the Oligopaint IF-FISH scheme to identify old versus new histone inheritance at single genomic loci in mitotic cells. (Left) A germarium containing a GSC in prometaphase, where the 3D chromatin structure may disguise local asymmetries at genomic regions of interest. (Top) A linearized gene of interest (GOI) displays local asymmetries. (Bottom) Two anaphase cells depict potential Oligopaint IF-FISH results. Maternal chromosomes are outlined in pink and paternal ones in blue. (Bottom Left) Probes recognize genes (magenta) that change their epigenetic state in a 2:2 ratio with biased old:new H3-enriched regions. In this instance, each duplicated sister chromatid from either maternal or paternal chromosome has an "agreement" on old (green, inherited by GSC indicated by an asterisk) versus new (red, inherited by the CB) H3. (Bottom Right) Probes recognize genes (cyan) that maintain their epigenetic state associated with more symmetric regions (yellow) that do not have a strong bias for either old or new H3. 