B. Whole-cell extracts from PHSL1-CDC20 ama1∆ CDC5-ha3 Ha3-SPO13 cells were subjected to α-Ha immunoblotting. Secondary antibodies were detected by near-infrared fluorescence imaging. Top, progression into metaphase I. Bottom, serial two-fold dilutions of the sample collected at t = 10 h in SPM. The Cdc5-ha3/Ha3-Spo13 ratio is ~20. Data information: data are representative of two independent experiments. 