Individual coat layers together with their constituents and their approximate distances from the plasma membrane are listed on the left. Legend to individual depicted proteins (human homologs in parentheses) is shown on the right. Regulators of actin polymerization Las17, Vrp1 and Bzz1, and endocytic pioneering proteins Ede1 and Syp1, which were shown to localize in membrane-proximal regions outside of the depicted endocytic coat (Mund et al, 2018), are not shown for clarity. Note that only few copies of individual coat proteins (presented at the endocytic site in dozens of copies; Picco et al, 2015; Sun et al, 2019) are shown. See the text for further details. 