E. Effect of CCL2 on KCl-induced MCH release. The perifusion of hypothalamic tissues with 60mM KCl for 30 min induces an up to 3.1-fold increase in MCH release. CCL2 (100 nM) dramatically blunts KCl-induced MCH release, an effect partially by the addition of INCB3344 (10µM) (3 to 5 independent experiments, 3 chambers per condition in each experiment). * or $ p<0.05, ** p<0.01, *** p<0.001. * KCl vs KCl+CCL2 conditions. $ KCl+CCL2 vs KCl+CCL2+INCB3344 conditions. Data are expressed as mean fold-increase in MCH release per chamber vs basal secretion, ± SEM. 