B. Left: averaged Nanoparticle Tracking Analysis displaying the size distribution of EVs isolated from media of MDA-MB-231 cells expressing Control (pcDNA3) or FLAG-RASSF1C plasmid. Inserts: transmission electron microscopy images of single EVs. Scale bars represent 200 nm. Right: quantification of total secreted EVs (96 h, top) or at indicated times (below), as measured by NTA. Data information: All data are from n=3 independent experiments. Data are analyzed by Student T-test and represented as mean ± SEM.* p ≤ 0.05, ** p ≤ 0.01, **** p ≤ 0.0001. 