(A) ssDNA repair efficiency of cells expressing RAD18 from the GAL1 promoter as determined from the maximal and final percentages of cells with Rad52 foci during the time course (right panel). Cells were synchronized in G1 from galactose-containing medium and released into S phase in the presence of 0.033% MMS for 1 hour, treated with 2.5% sodium thiosulfate to inactivate the MMS, washed and released into fresh medium for different times. Cells were shifted to glucose-containing medium 1 hour after MMS inactivation. The percentage of cells with foci at each point was normalized to the highest value of the wild type, taken as 100 (left panel). See Figure EV1A for control cells released in galactose-containing medium during the entire time course. Data information: The mean and SEM of 4 independent experiments are shown. Statistically significant differences according to an unpaired two-tailed Student's t-test are shown, where one, two and three asterisks represent p-values <0.05, <0.01 and <0.001, respectively. 