F) Images of Plin2-GFP in 3rd instar larval fat body cells of different genotypes. Scale bar represents 25 µm. (F) The localization of Plin2-GFP is greatly reduced in peripheral lipid droplets and increased in medial lipid droplets in CSN2 RNAi larvae fed on a 30% high-fat diet. DAPI (blue) stains nuclei. White arrows indicate peripheral lipid droplet. Red arrows indicate medial lipid droplets. 