(C) Image correlation analysis (ICA) of GlyR and GABAAR clusters at the same PSDs. The ICQ values between GlyR and GABAAR (blue trace, 0.36 ± 0.06, n = 395 synapses from three independent experiments) are significantly lower than between GlyR clusters labelled with two secondary antibodies conjugated with different fluorophores (yellow trace, 0.38 ± 0.04, n = 218 synapses; MW test, p < 0.0001). 