C. cdc20ts-mAR PDMC1-AMA1 PHSL1-CDH1 cells containing CDC28 or cdc28-as2 were released from metaphase I at t = 0 and shifted to 37°C at t = 50 min to cause arrest at metaphase II. 1Na-PP1 was added at t = 100 min to inhibit Cdc28-as2. Microtubules (GFP-Tub1), Mpc70-GFP, and RFP-Spo2051-91 were imaged in cells fixed at the indicated times. Images represent ≥90% of cells carrying the RFP-Spo2051-91-plasmid. Scale bar, 2 μm. Data information: data are representative of two independent experiments. 