A. Over-representation analysis was performed with Gene-Signatures highlighting activation of specific pathways using Gene Set Enrichment Analysis (GSEA) on microarray data obtained from control and mutant-p53 silenced MDA-MB-231 mammary cells. A negative normalized enrichment score (NES; highlighted in blue) indicates signatures that are more active in control cells, i.e., that have been repressed upon the silencing of mutant p53 proteins; a positive NES (highlighted in red) indicates signatures that have been activated by mutant p53 silencing (i.e., signature normally not active in MDA-MB-231). The false discovery rate (FDR) is the estimated probability that a gene set with a given NES represents a false positive; we considered signatures to be significantly enriched at FDR<0.05. Gene expression data has been obtained from n=3 biological replicates for each condition. See Appendix Table S2 for a GSEA analysis including also Biocarta gene sets. 