A. Traces showing average changes (mean±sem) in intracellular Ca2+ (Fura2 ratio) over time in response to perfusion of TG as indicated in the upper bar in SH-SY5Y S1-/- cells transfected with mcherry tagged constructs as indicated (n=46-105). B. Quantification of the Area Under Curve (AUC, mean±SD), calculated from traces shown in (A) with STIM1 (n=105), STIM1∆KK (n=56), STIM1A (n=75), STIM1A∆KK (n=46). ***p<0.001, Kruskal-Wallis Anova with Dunn's multiple comparisons test. Total n from three independent transfections with 2-3 technical replicates each. C 