b, Helical wheel projections of the red sequences from a. A schematic representation of the 7-bladed beta-propeller PROPPIN structure is also shown, illustrating the localisation of the putative amphipathic -helix (blue) of the CD loop between the two lipid binding sites for PI3P/PI(3,5) P2. Bold print marks phosphorylation sites in the loop of Pichia pastoris Atg18, which are relevant for its membrane insertion (Tamura et al, 2013). 