Experimental outline for in vivo CAR generation. 1x107 human PBMC were engrafted into naïve NSG mice or NSG mice that had been intraperitoneally (i.p.) injected with 5x105 Raji cells (Raji +) six days before. One day later, 2x106 t.u. of CD8-LVCD19CAR (filled circles) or CD8-LVRFP (grey triangles) were i.p. injected, respectively. As further control, another group of mice received PBS (open circles). Seven days later, mice were sacrificed and organs and cells were removed for further analysis. 