(B) The integrity of the isolated lysosomes. The release of β-hexosaminidase from lysosomes was indicated with incubation of lysosomes with para-nitrophenylphosphate (PNPP). The reaction was terminated with 0.25 mol/L NaOH. Release of all lysosomal β-hexosaminidase was induced by incubation with 0.1% Triton X-100. Release of all lysosomal β-hexosaminidase was induced by incubation with 0.1% Triton X-100. Values of β-hexosaminidase activity are expressed as percentage of total activity in the lysosomal fraction at time 0, 10, 20 and 30 min and are means±SE of three independent experiments. 