WT and Mfn2 KO MEFs were co-transfected with plasmids encoding: H) LAR-GECO1 and ER-Mfn2 or control (CT) plasmid. ER Ca2+ levels were determined. ER Ca2+ release was induced with caffeine (20 mM) as indicated (n= 35 cells from 4 independent experiments). Data are presented as mean ± SEM Data information: *p<0.05, one-way ANOVA followed by Tukey's post hoc test. Note that experiments were performed at the same time, so the values of WT and Mfn2 KO transfected with control plasmid are the same but the figures have been split in two for the sake of linearity. 