(f) Spatial peak: trough fold change in Venus::HES5 intensity profile in the D-V axis measured at 2h and 10h in DMSO and DBZ treated E10.5 Venus::HES5 spinal cord slices; dots indicate average over 3 z-slices from DMSO (n=3) and DBZ ( n=4) experiments; lines indicate median per condition; 1 tailed unpaired t test with significance *p<0.05. 