Isolation of dermal EC subtypes from the ear skin of 5 weeks old Prox1-GFP mice using multicolor flow cytometry. Whole-mount immunofluorescence for LYVE1 (on the left) defines three Prox1-GFP+ lymphatic vessels subtypes that are sorted using the indicated gating scheme (on the right): LYVE1low/- collecting vessel (green arrow, col); LYVE1intermed pre-collecting vessel (grey arrow, pre-col); LYVE1high lymphatic capillary (magenta arrow, cap). 