Schematic of puromycin-PLA assay. Cells were treated with DMSO, PPP or PPP + anisomycin for 30 min in the axonal compartment. After this puromycin was added to the medium for an additional 30 min. Cells were then fixed and the PLA protocol was performed (see Materials and Methods). Puromycin- Purple triangle, BICD1-Grey circles, Anti-puromycin antibody- green, Anti-BICD1 antibody- blue, Secondary antibodies- black. 