B. RT-qPCR analysis of Rbm3, Mbnl2 and Sf3b3 in shScr, sh1 and sh2 ESCs. mRNA expression is relative to shScr and presented as mean ± SEM of n ≥ 3 independent biological experiments; *P < 0.05, **P < 0.01, ***P < 0.001 (shScr vs. sh1 or sh2); unpaired Student's t-test. 