B. Rho-GTP pull-down binding assay in MDA-MB-231 control or RASSF1C over-expressing cells and co‑transfected either with SRC plasmids, siRNA against SRC or siNT (non-targeting sequence). Western blot analysis shows Rho activity (Rho GTP-double band) and pMCLII and pRhoGDI binding. The ratio below Rho-GTP Western blot shows differences in Rho-GTP protein expression, compared to control condition. Data information: For both Rho-GTP pull-downs and immunoprecipitation assays total proteins were used as loading controls. All data are from n=3 independent experiments. Data are analyzed by Student T-test, represented as mean ± SEM. *** p ≤ 0.001. 