Pom1as1-tdTomato in cells co-expressing Pom1-GFP (left) or Pom1KD-GFP (right) before and 1-2 min after addition of 3MB-PP1, as indicated. Before drug addition, Pom1 is strongly enriched at cell poles. After drug addition, Pom1as1-tdTomato is rapidly delocalized around the entire cell periphery (arrowheads) in cells co-expressing inactive Pom1KD-GFP, but not in cells co-expressing active Pom1-GFP, suggesting intermolecular phosphorylation (Hachet et al, 2011). Medial confocal planes are shown. Scale bar is 5µm. 