B) The upper panel illustrates the location of the conserved and non-conserved (-nc) predicted microRNA binding sites in the 3'UTR of 3 selected target genes. The bar graph compares the normalized expression of a firefly luciferase reporter (luc) plasmid (EV) after transfection in 293T cells and the same luc reporter with a modified 3'UTR containing predicted microRNA binding sites. The values represent the ratio of luc expression when the reporter was co-transfected with synthetic miR-424 and miR-503 vs. a control non-targeting sequence. The bars with a diagonal line pattern indicate non-conserved sites. A known miR-424/503 binding site located in the 3'UTR of CDC25 is used as a positive control. The horizontal line indicates 25% of reduction in luminescence and it is included as a visual reference. The error bars show the standard deviation. 