ClustalO (Sievers et al, 2011) multiple sequence alignment of various yeast Sir4 proteins focusing on the putative new structural domain and comparison with S. cerevisiae Dbf4 H‑BRCT. Sir4 sequences: ScSir4 (Saccharomyces cerevisiae, Uniprot P11978), NdSir4 (Naumovozyma dairenensis, G0W8P4), NcSir4 (Naumovozyma castellii, G0VBN1), KnSir4 (Kazachstania naganishii, J7S2D5), KaSir4 (Kazachstania africana, H2AVL7). 