(E) SMAD3 and SLC9A5 gain-of-function increases invasion capability of melanoma cells. Invasion assays for engineered cells lines (melanoma spheroids): CTR; 501Mel 2+, SMAD3; 501Mel 2+cells overexpressing SMAD3. Two other cell lines overexpressing SLC9A5 or BIRC3 have been tested. Explanation for ratio calculation is detailed in Fig. EV3A. Results obtained from two biologically independent experiments. (n=5, 4, 6 and 3 spheroids, respectively). 