Top, general overview of the non-rotated IAPV-IRES/80S class 1 structure. IAPV-IRES is depicted as solid Van Der Walls surface colored according to the secondary structure motifs. The PKI (green) is solidly anchored to the A site. Bottom, close up view of the IAPV-IRES PKI inserted in unsharpened map. Zoomed view of A3760, a nucleotide belonging to the helix 69 (H69) of the 28S rRNA interacting with PKI. Final refined model inserted in the post-processed map is shown. This interaction is not disrupted along the small fluctuations of the 40S. An apical view along the axis of the PKI of a superposition of class 1 versus class 2 shows the IRES displacements are minimal and are followed by the H69 which constantly interacts with the IRES. 