(C) Chemical shift perturbation values and relative peak intensities for backbone resonances (15N and 1H) of YqgPNTD calculated for complexes of 400 μM YqgPNTD with 10 μM (light grey, light orange) or 40 μM MnCl2 (dark grey, dark orange bars). Only residues 20-100 of YqgP are displayed for simplicity. Residues identified as potential manganese-binding region based on their chemical shifts and intensity changes upon NMR titrations are depicted in orange. The X sign marks residues whose chemical shifts were not calculated due to the lack of resonance signal. 