WT and Mfn2 KO cells were transfected with plasmids encoding: F) LAR-GECO1 and ChiMERA or control (CT) plasmid. ER Ca2+ was determined. ER Ca2+ release was induced with caffeine (20 mM) as indicated (n= 30 cells from 3 independent experiments). Data are presented as mean ± SEM. 