D Immunoblot analysis and quantification of LC3 in HCT116 cells (mean ± SEM). Cultured cells were treated with DMSO (Control (Ctrl)), NEN or NEN and Domperidone (NEN+Domp) as indicated (NEN, 1.2 µM; Domp, 30 µM) for 16 h, followed by control or 20 µM chloroquine treatment for 3 h. Protein band signal intensities were normalized to vinculin (loading control) and the ratios LC3-II bands in presence or absence of chloroquine treatment (N=6) were analyzed by Kruskal-Wallis with Dunn´s post-hoc test. * p< 0.05; *** p<0.001. 