(D) Chart comparing the expression of eight homeodomain TFs in the four anterior MNs (MN2c, MN2a, MN4, MN3) as identified by scRNA-Seq (Seq) and immunohistochemistry (IF/HCR), with absence of expression color-coded in magenta and presence of expression in green. The genes were selected for validation, as they allowed an unambiguous distinction of the four anterior MNs according to the scRNA-Seq data. 