A, B. Imaging of SPBs (Cnm67-RFP) and Spc72-GFP in ndt80∆ cells expressing PEST-IME2-∆C and/or PEST-CDC5 upon addition of estradiol (EST) at 4 h in SPM (t = 0). Top, time-lapse series. Bottom, quantification of cells with Spc72 at SPBs. (A) ndt80∆ cells expressing Ime2-∆C or Cdc5. (B) Expression of Ime2-∆C and Cdc5 in ndt80∆ cells containing wild-type Spo13 or the phospho-site mutants Spo13-10A and Spo13-10D. While Spo13-10D delays Spc72 removal by 127 min (95% CI, 105-149; P < 0.0001), Spo13-10A has little effect (95% CI, -24-0.8; P = 0.07; Welch's t-test). Data information: data are representative of two (A) or 2-4 (B) independent experiments. 