E. Quantification of the absolute number of NANOG positive cells (measured as average cell number/spheroid) per MDA-MB-231 ZsGreen (control) or ZsRASSF1C (RASSF1C) spheroids in 3D-collagen, treated with ROCK inhibitor (Y27632, 10 μmol/L) or Rho inhibitor (C3, 2 μg/ml). Data Information: All data are from n=3 independent experiments. Data are analyzed by Student T-test and represented as mean ± SEM.* p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001. 