RNAPII CRAC were performed in the presence of the indicated versions of SEN1 expressed from its own promoter in a centromeric plasmid, upon depletion of the chromosomally-encoded version of Sen1 in a Sen1-AID strain. Venn diagrams illustrating the overlap between the CUTs that display statistically significant termination defects (p-value≤ 0.05, see methods for details) in the different mutants. 