(B) Representative confocal fluorescence micrographs of HeLa cells co-transfected for 24 h to produce GFP-TGN38 and mCherry-BspF and stained for F-actin with AlexaFluor™647-phalloidin. Cells were left untreated or treated with Cytochalasin D (200 nM) for 30 min prior to fixation. Scale bars: 10 and 1 µm (insets). 