(E) HCR for DIPgamma and DIPkappa transcripts in Drosophila stage 17 embryos. RNA expression of both genes is analyzed in control (elav>GFPRNAi) versus Dfd depleted (elav>DfdRNAi) animals, the ventral nerve cord is highlighted by a white dashed line, the Dfd expressing subesophageal ganglion (SOG) is indicated by a yellow circle. 