A, TOC1 protein expression pattern in 3-d-old seedlings in native promoter TOC1/5X/T135A/S175A lines grown in SD. White and black regions indicate light and dark period. Data from 3 independent trials were averaged; error bars indicate SEM. Statistical analyses were performed by Students's t test. Asterisks indicate significant differences between TOC1 wild type and its phosphosite mutants (*, p<0.05). Data information: TOC1 protein was detected by α-GFP, ADK was used as loading control. TOC1/ADK ratio was calculated by the intensities of TOC1-GFP bands normalized by the intensities of ADK bands using ImageJ. 