(D) Quantification of Ig gene expression changes after interference with Dfd expression in neuronal cells (elav>DfdRNAi) by qPCR. Two genes, DIPgamma and DIPkappa, were selected as potential Dfd targets based on their co-expression in Dfd positive MNs. The bar chart shows the fold enrichment after normalization against Act5C of the Dfd and Ig genes in control versus elav>DfdRNAi conditions from three biological replicates, and shows that Dfd and DIP-kappa expression are significantly reduced in elav>DfdRNAi embryos, whereas the expression of the homeodomain TF gene exd is unchanged (internal control). p-values were calculated from a t-test: *<0.05, **<0.01, ns non-significant. Error bar denotes standard deviation. 