F. Top, schematic of RASSF1C domain structure indicating putative mutations in the RA domain affecting RhoA activity. Bottom, immunoprecipitation of MDA-MB-231 transiently transfected with either control (DsRed), DsRASSF1C plasmid, DsRASSF1C-R197W or DsRASSF1C-R199F mutants. Pull‑down was performed using a SRC or same species IgG antibody and blotted with indicated antibodies. Data information: For both Rho-GTP pull-downs and immunoprecipitation assays total proteins were used as loading controls. All data are from n=3 independent experiments. Data are analyzed by Student T-test, represented as mean ± SEM. *** p ≤ 0.001. 