F. Quantification of the absolute number of NANOG positive cells (measured as average cell number/spheroid) per MDA-MB-231 ZsGreen (control, con) or ZsRASSF1C (RASSF1C) spheroids in 3D-collagen, co‑transfected with either RhoA WT, catalytically active (CA) or dominant negative (DN) constructs. Data Information: All data are from n=3 independent experiments. Data are analyzed by Student T-test and represented as mean ± SEM.* p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001. 