A Scheme of suggested topology of Sla1, Ent1, Sla2 and Pan1(1-1303) proteins on the flat (left) and invaginated (right) plasma membrane. Natively unfolded parts of the proteins are shown as thick black lines. The C-termini of proteins showing FRET are depicted as yellow circles; the C-termini separated from their FRET partners during invagination are shown as gray circles. The position of the clathrin lattice and polymerizing actin cytoskeleton are shown as a pale orange and red zone, respectively. 