(G) Effects of knockdown and overexpression of Hsc70 on Htt-552 levels. PC12 cells were transiently transfected with human Hsc70 plasmid, vector control or Hsc70 siRNA for 72 h and Htt-552 for 48 h. Lysates were subjected to Western blot analysis with anti-Htt 2166, anti-Hsc70 antibodies. Values are the mean±SE of three independent experiments. **P<0.01 (compared with Htt-552-18Q without treatment); ##P<0.01 (compared with Htt-552-100Q without treatment). 