Pom1 amplitude increases with Tea4 concentration at the pole. Linear regression (black line) in the log-log scale shows a slope smaller than one indicative of a sub-linear relationship between Pom1 and Tea4 at the pole (see also Fig. 2B) and thus buffering of Tea4 fluctuations. Each dot corresponds to one average Tea4 and corresponding Pom1 profiles as in (C-D). The trans-phosphorylation model predicts the slope to be 2/3 (red line), close to the observed 0.63 (black line) and within the two SE confidence interval (shaded area). Each data point is weighted by its inverse variance of the mean in the linear regression. 