(C) A GST-pulldown assay was performed through incubating GST-tagged GSDMD and mutants with His-tagged nucleocapsid, followed by immunoblotting with antibodies against the indicated protein tags. GST-GSDMD, GST-GSDMD mutants and His-nucleocapsid were purified from E. coli strain BL21. Data information: Experiments were repeated for three times with similar results. 