 (A) Experimental setup for scRNA-seq analysis. E11.5 CD133+ spinal cord progenitor cells were isolated using magnetic cell sorting (MACS) whereas E15.5 cells were randomly isolated from dissociated spinal cord tissue. Bioinformatic analyses, including t-SNE, revealed clustering of 350 E11.5 CD133+ progenitor cells and E15.5 cells. Colors in the graph indicate cell origin (pink for E15.5 cells and brown for E11.5 cells) 