(G) An in vitro cleavage assay was performed by incubating GSDMD and caspase-1 p20/p10 complex with or without the presence of nucleocapsid, followed by immunoblotting with antibodies against the indicated protein tags. FLAG-GSDMD was immunoprecipitated from HEK293T cells using anti-FLAG coupled magnetic beads. His-tagged p20, p10 and nucleocapsid were purified from E. coli strain BL21. 