C. Same as B. except that cells stably expressing GFP-NLS-CDKL5 were pre-incubated with DMSO (mock), olaparib (5 μM) or PDD00017273 (0.3 μM) for 1 h prior to spot micro-irradiation (405 nm). Individual cells from one of two independent biological replicates is shown. Scale bar is 10 μm 