(f) Example cross-correlation function of Venus::HES5 with NGN2::mScarlet-I (blue), Venus::HES5 with brightfield signal (black), and NGN2::mScarlet-I with brightfield signal (red) from the same transverse slice of E10.5 spinal cord; markers indicate the presence of two types of co-ordination namely in-phase (red arrowhead) and out-of-phase (black arrowhead). 