A. PHSL1-CDC20 ama1∆ cdc28-as2 cells were treated with 1Na-PP1 at t = 3 h in SPM to inhibit Cdc28-as2. At t = 5 h (left) or t = 9 h (right), Cdc28-as2 was activated by washing cells with conditioned SPM (cSPM) lacking 1Na-PP1 (washout). Top, time-lapse series from the imaging of Mpc70-GFP and RFP-tubulin. The weak, nuclear signal from Ndt80-GFP serves to confirm entry into meiosis. Middle, quantification of spindle formation and Mpc70 loading to SPBs. Bottom, quantification of control experiments. Cells were washed with cSPM plus 1Na-PP1 at t = 5 h (left) or t = 9 h (right) in SPM. 