qRT-PCR measurement of of Ifnb1 mRNA transcripts in mouse peritoneal macrophages left unstimulated or stimulated with LPS (0.1 μg/mL) in the presence of DMSO or Palbociclib (10 μM) for indicated times. The data shown are mean ± SEM of n=3 biological replicates. Statistical significance was determined by two-way ANOVA followed by Tukey's post hoc test. ns, not significant. 