(F) HeLa cells were transfected with EGFP-tagged constructs of both mouse Nup50 orthologues and the Nup50B Q177D (orthologue to the position 178 in Xenopus) mutant. After 24h, the cells were analyzed by live cell imaging. Panel shows cells exiting mitosis (time normalized to metaphase-anaphase transition). The merge shows histone 2B in pink and Nup50 in green, the black and white panel shows EGFP-Nup50 signal. Scale bar: 10 µm. 