A Experimental design for the mouse model in Fig. 8. Control mice (p53flx/flx) and liver specific Alb-Cre p53 knockout mice (Alb-cre p53flx/flx) (n=5) were injected with a single dose of DEN at 13 days and then fed with HFD diet at 4 weeks. A tail vein injection of an associate adenovirus serotype 8(AAV8) expressing either control or SQLE shRNA at 13 weeks as indicated. p53flx/flx and Alb-cre mice were examined by genotyping (right panel). p53 flx/flx product size: 370bp. p53+/+ product size: 288bp. Alb-cre product size: 385bp. 