(A) Plasmids encoding FLAG-tagged full-length GSDMD were co-transfected with plasmids encoding Myc-tagged SARS-CoV-1 nucleocapsid or SARS-CoV-2 nucleocapsid into HEK293T cells for 24 h, followed by immunoprecipitation with antibody against Myc or isotype IgG. Input and precipitates were immunoblotted as indicated. 