C, A549 and H358 cells expressing mCHerry-GFP-LC3B were treated with vehicle (DMSO), AZD 4547 (5 µM) and BI2536 (5 nM), alone or in combination for 24h before flow cytometry-based analysis for mCherry and GFP (upper). Shown underneath is quantification of the data. **P<0.01 and ***P<0.001 by two-way ANOVA with Tukey's multiple comparisons test. Data are shown as mean ± SD (error bar) of three independent experiments (n=3). 