(D) Recruitment of NRF1 to TMEM33 promoter analyzed by ChIP-qPCR in parental and PKM2 KO MCF7 cells. IgG was used as a negative control. NRF1 enrichment on the TMEM33 promoter region was normalized to IgG control (n=3). Data information: Data are displayed as means ± SD. Statistical significance was assessed using two-tailed Student's t-test, ∗∗p < 0.01 and ∗∗∗p < 0.001. 