D. Model representing how RASSF1C expression in RASSF1A‑silenced breast cancer cells (MDA-MB-231) activates a signaling cascade involving SRC kinase, RhoGDI and culminates in RhoA activation and generation of amoeboid contractility and cancer stemness features. RASSF1C-driven amoeboid cancer stem cells contribute to alteration of the tumor microenvironment by rendering recipient cells (MCF7, T47D) more invasive and able to upregulate stem cell markers, due to EV-mediated communication. 