(D) Schematic diagram of Plin1, Plin2 and the Plin2-Plin1-C chimeric protein. Lysates of Drosophila S2 cells expressing the different proteins were immunoprecipitated with anti-Flag antibody and then probed by anti-GFP or anti-Flag antibody in a western blot. Neither Plin1 nor Plin2-Plin1-C chimeric protein coimmunoprecipitated with RDH/CG2064. 