qRT-PCR measurement of of Ifnb1, Il6 and Il1b mRNA transcripts in mouse peritoneal macrophages left unstimulated or stimulated with LPS (0.1 μg/mL) (A-C) or transfected with Poly(I:C) (1μg/mL) The data shown are mean ± SEM of n=3 biological replicates. Statistical significance was determined by two-way ANOVA followed by Tukey's post hoc test. ns, not significant. 