(E, F) GSDMD+/+ and GSDMD-/- THP-1 cells stably expressing nucleocapsid or control vector were stimulated with 10 μM nigericin for 30 min following pretreatment with 1 μg/ml LPS for 3 h. Supernatants were collected for an ELISA assay to determine IL-1β levels (E). Cell pellets were subjected to a cell viability assay through checking cellular ATP levels (F). For E-F, Student's t-test was used. Data were shown as means±SD of three technical replicates. NS, non-significant. Results were repeated for three times with similar results. Data information: control vector used here was empty vector corresponded to the nucleocapsid-containing vector. Student's t-test was used and data were shown as means±SD of three technical replicates. **, P<0.01; ***, P<0.001; NS, non-significant. Similar results were observed for at least three times. 