D) Genes at which the TSS interacted with a HIF-binding site and E) genes adjacent to HIF-binding sites, which did not interact with the site were subjected to GSEA using a ranking metric combining fold-change in hypoxia and significance[25]. The ranking metric on the horizontal axis is shown as a color scale at the bottom. All the genes whose promoters were associated with HIF-binding sites appeared amongst the most strongly hypoxia-induced genes, whilst non-interacting genes showed no enrichment. 