Fluorescence Recovery After Photobleaching (FRAP) experiment on AcGFP-Siwi and AcGFP-Siwi-D670A. Red dotted lines represent recovery traces of Siwi-D670A foci (n=36) and black dotted lines represent recovery traces of Siwi foci (n=36). Solid lines represent locally estimated scatterplot smoothing (LOESS) curve and the grey areas represent 95% confidence level interval. Fluorescence intensity is quantified with the softWoRx software and normalized to minimum and maximum in a 0-1 scaling. 