G - J) Statistical analyses of mitochondrial transport parameters of five independent experiments shown in (D-G and Fig. EV3A-D). Each bar represents the mean (±SEM) of medians of single experiments for (G) run length and (H) speed distributions, and average (±SEM) (I) overall mitochondrial motility and (J) density of all five experiments. Values of fold change between control and Ttll1‑/- and Ttll7-/- conditions are indicated below the graphs. Full bars are for anterograde, and empty bars for retrograde transport events. Significance was tested using unpaired t-test. Average (G) run length as well as (H) speed showed no significant differences between control and Ttll1-/- and Ttll7-/- conditions. (I) The averages of overall motilities reveal an about 1.7-fold increase in overall mitochondrial motility in Ttll1-/- neurons, while no significant differences were found in Ttll7-/- neurons. (J) Mitochondrial density was unaltered between control and knockout conditions. Total number of mitochondria analysed from five independent experiments: control (1260), Ttll1-/- (1158) and Ttll7-/- (1191). 