(C) HEK293T cells were transfected with both Nup50 mouse orthologues N- and C-terminally tagged with EGFP. 24h post transfection cells were lysed, EGFP-tagged proteins immuno-isolated and analyzed by western blotting. The quantification of the western blot (C, lower panel) shows the mean signal intensity normalized on EGFP of three independent experiments. Data points from individual experiments are indicated. 