(A) rBCV biogenesis in BMMs infected with either wild type (2308), VirB-deficient (ΔvirB11), ΔbspF, or complemented ∆bspF (ΔbspF::bspF) bacteria, measured as the percentage of LAMP1-positive BCVs over time. Data are means ± SD of n=3 independent experiments. Asterisks indicate statistically significant differences (P<0.05, two-way ANOVA followed by Dunnett's multiple comparisons test) compared to control (2308). 