A) hnRNPA2 LC (AlexaFluor488-tagged, green) undergoes LLPS, while hnRNPF PLD (AlexaFluor555-tagged, red) does not. However, hnRNPF PLD partitions into hnRNPA2 LC droplets when mixed at a 1:1 ratio. Conditions: 20 µM indicated protein (~1% fluorescently tagged), 20 mM MES pH 5.5, 50 mM NaCl, 150 mM urea. Scale bar: 10 µm. B) At 300 µM, FUS LC (AlexaFluor488-tagged, green) undergoes LLPS, but at 200 µM hnRNPF PLD (AlexaFluor555-tagged, red) still does not undergo LLPS. When mixed at 300 µM FUS LC and 200 µM hnRNPF PLD, hnRNPF PLD does not partition into FUS LC droplets. Conditions: 300 µM FUS and 200 µM hnRNPF PLD (~1% fluorescently tagged), 20 mM MES pH 5.5, 150 mM NaCl, 150 mM urea. Scale bar: 10 µm. 