(D) Genotyping of CRISPR/Cas9 generated TMEM33 knockout mice using fluorescent probes for PCR. FAM+, Cy5- indicates the TMEM33+/+ genotype; FAM+, Cy5+ indicates the TMEM33+/- genotype; and FAM-, Cy5+ indicates the TMEM33-/- genotype (n>=5). 