D) The western blot shows the difference in nuclear accumulation of β-catenin when dox-induced (100ng/ml for 3 days) miR-cluster-Dox-MCF-10A cells (miR) and miR-control-Dox-MCF-10A cells (EV, empty vector) were exposed to of WNT3a ligand (100ng/ml for 6 hours). The numbers below the blots indicate the quantitation by densitometry of protein expression relative to EV without WNT3A. Histone H1 staining is shown to control for nuclear-cytosolic purification. The upregulation of miR-424 and miR-503 when dox was added to the media was quantified by QRT-PCR and is shown in the bar graph. The error bars show the standard deviation. 