Distribution of SiE5′NT orthologues across higher fungi. End nodes are color-coded based on the presence (blue) or absence (red) of 5′NT genes in a particular fungal taxa. Numbers in parentheses besides the nodes specify the number of species that have 5′NT genes with respect to the total number of genomes analyzed. Left tree: distribution of 5′NT without signal peptide (SP) and GPI anchor. Right tree: E5′NT with SP and GPI anchor. The distribution shows that E5′NT genes are mostly present in Ascomycota such as Sordariomycetes (107/121), followed by Dothideomycetes (24/37) and finally Leotiomycetes (6/18). Few species of Eurotiomycetes (6/118) possess an E5′NT orthologue. In Basidiomycota, E5′NT members are only found in the class of Agaromycotina (12/85). 