(D) Average of ER-mitochondria distance (n= 111-168 mitochondria). *p<0.05, Mann-Whitney test. Data are presented as mean ± SEM. (E) Quantification of the percentage of mitochondria in contact with the ER distributed at the indicated distance range (n= 3 independent experiments in which were analyzed 627-779 mitochondria from 68-98 cells). Data are presented as mean ± SEM. *p<0.05, two-tailed Student's t-test. Data information: Note that experiments were performed at the same time, so the values of WT and Mfn2 KO are the same but the figures have been split in two for the sake of linearity. 