(L, M) (L) MMP potential was measured in WT and Mfn2 KO cells treated with vehicle or methyl-pyruvate (5 mM) for 24 h (n= 3 independent experiments). *p<0.05, one-way ANOVA followed by Tukey's post hoc test. (M) Effect of methyl-pyruvate in mitochondrial Ca2+ uptake after caffeine stimulation (n= 30 cells from 3 independent experiments). Data are presented as mean ± SEM. 