 (A) Schematic illustration of two alternative approaches to control myosin-II activation in a curved tissue. In order to restrict the region of activation in a curved tissue to the basal surface using a uniformly distributed optogenetic anchor such as the CIBN-pm anchor (left), the photo-activation pattern needs to account for the tissue curvature and the region of activation needs to be corrected in 3-D using adaptive optics and holography. An alternative approach is to engineer an optogenetic anchor that specifically localizes to the cell base. In this scenario, a bigger focal volume can be photo-activated while still resulting in the restricted recruitment of RhoGEF2-CRY2 to the cell base 