(C) and (D) Effects of knockdown and overexpression of LAMP-2A on Htt-552 levels. PC12 cells were transiently transfected with LAMP-2A plasmid, vector control or LAMP-2A siRNA for 72 h and Htt-552 for 48 h. Lysates were subjected to Western blot analysis with anti-Htt 2166, anti-human LAMP-2A antibodies. Values are the mean±SE of three independent experiments. *P<0.05 (compared with Htt-552-18Q without treatment); #P<0.05 (compared with Htt-552-100Q without treatment). 