Top: western blot assays showed level of ASS1 in SW-1116 cells stably expressing LOC113230 or vector treated with cycloheximide (CHX, 20 μg/mL) or vehicle for the indicated periods of time. Bottom: the gray scale-analysis of ASS1 level was conducted and GAPDH protein level was used as a loading control. Results are shown as means±SD. SW-1116 cells stably expressing LOC113230 or vector were treated with MG132 (5 μM) or vehicle for 4, 8 or 12 hrs, respectively. Cell lysates were analyzed by western blotting assays. 