(B) Recruitment of Stx6-positive vesicles to rBCVs (expressed as percentage of Stx6-positive BCVs) in BMMs infected for 24 h with either wild type (2308), ∆bspF or complemented ∆bspF (∆bspF::bspF) bacteria. Data are means ± SD from n=3 independent experiments, in which at least 300 BCVs were analyzed per experiment via CellProfiler image analysis. Asterisks indicate statistically significant differences compared to 2308-infected BMMs as determined by one-way ANOVA with Tukey's multiple comparisons test (P < 0.05). 