B-E. Study of gene and protein hypothalamic expression of the chemokine CCL2 (B, C) in icv LPS injected mice. at different times after injection (from 1h to 24h), normalized to values in control icv saline injected mice (n=6 per group). Measurement of CCL2 (C) ) concentration by EIA after icv LPS injection (black bars) or icv saline injection (grey bars) in mice at 1h, 6h and 18h after injection. 