D. Metabolic labeling to monitor PC and PE synthesis. Equal number of WT (3D7) and PfCDPK7-KO (KO) parasites were incubated with 14C-Eth or 14C-Cho (in RPMI) for 12h. Subsequently, parasites were harvested and lipids were extracted and separated by thin layer chromatography (TLC). The radiolabeled lipids were detected by phosphorimaging (upper panel). Bottom Panel, Radiolabelled PC and PE were quantitated by performing densitometry on phosphorimage of TLC plates in above panel and % change in PC or PE formation in PfCDPK7-KO parasites with respect to 3D7 (100%) was calculated (mean ± SEM, n=4; biological replicates, **p<0.01, ANOVA; ns, nonsignificant). 