A The sequences of the isolated VHH antibodies (49 anti-RBD and 10 anti-S1ΔRBD nanobodies) were aligned using Clustal Omega (Sievers et al., 2011). The circular phylogram was reconstructed with Dendroscope (Huson and Scornavacca, 2012). Where applicable, sequence classes are indicated. The anti-RBD nanobodies are colored according to the two RBD epitopes identified in Fig 5 (with epitope 1 in green, epitope 2 in red, and asterisks marking nanobodies included in Fig 5). Anti-S1ΔRBD nanobodies are colored in magenta. VHH antibodies that did not neutralize SARS-CoV-2 are shown in parentheses. 