(C) Inhibition of degradation of Htt-552-18Q with mutation of CMA recognition sites. The degradation of Htt-552-18Q in HeLa cells transiently transfected with human Hsc70 plasmid or vector control and Htt-552-18Q▴ was determined with Western blot analysis. Values are the mean±SE of three independent experiments. **P<0.01 (compared with the expression of Htt-552-18Q without treatment). 