(A,B) Level of expression of borgs and septins in young and aged LT-HSCs. mRNA levels of borgs and septins were normalized to the level of expression of GAPDH and borgs are shown relative to the level of Borg 2 expression in young LT-HSCs. Septins are shown relative to the level of septin 7 expression in young LT-HSCs. At least three biological replicates (n=3) are shown. Error bars indicate mean±SEM, unpaired t-test, **P<0.01, ***P<0.001, ****P<0.0001. 