Workflow of the SILAC approach. 3T3-F442A cells were seeded and differentiated in the presence of heavy amino acids. After 14 days of differentiation, the EV secreted by the mature labeled adipocytes were isolated and analyzed by mass spectrometry to evaluate the presence of heavy amino acid-containing proteins. These EV were also added to SKMEL28 cells for 12h, then LC-MS/MS analysis was performed to identify heavy amino-acid containing proteins that had been transferred from adipocytes to melanoma cells via EV. 