(H) Plasmids encoding DNA binding domain (BD)-tagged nucleocapsid truncations and activation domain (AD)-tagged GSDMD C-terminus truncations were co-transfected into yeast strain AH109 and double positive clones were grown on plates containing X-α-gal. The intensity of blue color was calculated and shown as scores for both dot size and color. Plasmids encoding BD-p53 and AD-large T antigen were co-transfected as a positive control. 