http___medlib.mef.hr_1685__2.xml
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Demirović, Alma and Džombeta, Tihana and Tomas, Davor and Spajić, Borislav and Pavić, Ivana and Hudolin, Tvrtko and Milošević, Milan and Čupić, Hrvoje and Krušlin, Božo (2010) Immunohistochemical expression of tumor antigens MAGE-A3/4 and NY-ESO-1 in renal oncocytoma and chromophobe renal cell carcinoma. Pathology - Research and Practice, [Epub . Abstract The distinction between renal oncocytoma (RO) and chromophobe renal cell carcinoma (ChRCC), especially the eosinophilic variant, can often be difficult. Our study has documented for the first time the expression of MAGE-A3/4 and NY-ESO-1 cancer testis antigens (CTAs) in these tumors. A total of 35 patients (17 ROs and 18 ChRCCs) were included in the study. Two antibodies were used for immunohistochemical staining: 57B recognizing multiple MAGE-A and D8.38 recognizing NY-ESO-1 CTAs. Fifteen (88.2%) samples of RO stained positively for both MAGE-A3/4 and NY-ESO-1 antigens. Regarding ChRCC, seven (38.9%) stained positively for MAGE-A3/4 and six (33.3%) for NY-ESO-1 antigens. Median MAGE-A3/4 expression was moderately positive in RO and negative in ChRCC. The difference in MAGE-A3/4 expression between two tumor groups was significant (P=0.0013). Median NY-ESO-1 expression was strongly positive in RO and negative in ChRCC. The difference in NY-ESO-1 expression between two tumor groups was also significant (P=0.0008). Our study has shown that RO had a significantly higher expression of both CTAs. However, additional research is needed to clarify their potential diagnostic implications. Actions (login required) Downloads Downloads per month over past year
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Bujas, Tatjana (2009) Imunohistokemijska analiza izraženosti antigena MAGE-A3/4 i NY-ESO-1 u metastatskom karcinomu pločastih stanica jednjaka. PhD thesis, Sveučilište u Zagrebu. Abstract Squamous cell cancer is the most recent malignant esophageal cancer and makes up approximately 6% of all malignant cancers of gastrointestinal system, however it has a highly unfavorable prognosis and five year survival rate is less than 10%. As the therapy (surgery, radiotherapy, chemotherapy) up till now has given poor and actually overwhelming results, numerous researches are under way with the scope of detecting new methods of treatment. Antigens associated to tumour, so called tumour associated antigens-TAA, are specific antigens able to recognize cyitotoxic T lymphocyte within the main system of the gene tissue tolerance (Major Human Leukocyte Antigens-HLA) type I. The most important among antigens are „melanoma-associated antigens“(MAGE) and antigen NY-ESO-1 which discovery gave necessary momentum to the attempts of applying immunotherapy. In this study immunohistochemical method was used for analyzing the expression of the tumour antigens MAGE-3/4 and NY-ESO-1 in the samples of esophageal squamous cells cancer and their spreading to the lymph nodes, in order to determine the link between expression of individual antigens in primary lesion and in spreading to the lymph node, and compare the expression link of each antigen with hystological tumour grade, TNM classification, site and size of tumour, age and sex of the patient. It has been established that the antigen MAGE-A3/4 expression positively correlates with NY-ESO-1 expression in the same tumour (r=0.310), and antigen NY-ESO-1 expression positively correlates with the same in lymph node metastases (r=0.605, rho= 0.588). Finally it has been established that the only parameter displaying serious statistic importance in comparison to the antigen NY-ESO-1 expression is the age of a patient, negatively correlating with antigen expression in tumour (P=0.001, r= - 0.516, , rho= - 0.515). Abstract in Croatian Karcinom pločastih stanica najčešći je zloćudni tumor jednjaka i čini oko 6% svih zloćudnih novotvorevina probavnog trakta, međutim ima vrlo nepovoljnu prognozu i visoki postotak smrtnosti, a petogodišnje preživljenje je manje od 10%. Češći je u muškaraca, javlja se u prosječnoj dobi oko 65-te godine života. U većem dijelu svijeta incidencija se kreće od 2,5 do 5 na 100 000 kod muškaraca te 1,5 do 2,5 kod žena. Postoji niz čimbenika rizika od kojih su najvažniji konzumiranje alkohola, pušenje cigareta, urođene greške (ahalazija), autosomno dominantne nasljedne bolesti (Tylosis palmaris), Plummer-Vinsonov sindrom, netropska sprue, strikture koje nastaju nakon ingestije lužina i kiselina, nedostatak vitamina, prethodno zračenje tog područja te u rizičnim područjima hrana koja je bogata nitritima i nitrozaminima, humani papiloma virus (HPV). Klinički simptomi karcinoma jednjaka se u 50 do 70% slučajeva javljaju tek u uznapredovaloj fazi bolesti, a najčešći su: disfagija (poteškoće gutanja), tupa bol u prsištu koja se širi prema kralježnici, gubitak težine i opća slabost. Kako je terapija (kirurška, zračenje, kemoterapija i endoskopske metode) do sada pokazala prilično loše i porazne rezultate, traži se u brojnim istraživanjima nova metoda liječena. Tumorskim antigenom može se smatrati bilo koja molekula iz tumorske stanice koju imunološki sustav prepoznaje kao stranu, odnosno na koju može nastati protutumorska imunološka reakcija. Tumoru pridruženi antigeni, tzv. (Tumor Associated Antigens-TAA), su specifični antigeni koje prepoznaju citotoksični T limfociti u okviru glavnog sustava gena tkivne snošljivosti (Major Human Leukocyte Antigens-HLA) tipa I. Najznačajniji među njima su antigeni koji čine tzv. „melanoma-associated antigens“ (MAGE) i antigen NY-ESO-1. Otkriće tih antigena dalo je novi zamah u pokušaju imunoterapije tumora. U ovom istraživanju imunohistokemijskom metodom analizirana je izraženost proteina MAGE-3/4 i NY-ESO-1 u uzorcima karcinoma pločastih stanica jednjaka i u njihovim metastazama u limfni čvor kod 55 bolesnika, kako bi se ustanovilo postoji li povezanost izraženosti tih proteina u tumoru s metastazom u limfni čvor i bez metastaze, kako bi se ustanovilo postoji li povezanost izraženosti pojedinog proteina u primarnom tumoru i u metastazi u limfnom čvoru, te kako bi se ustanovilo postoji li povezanost izraženosti svakog od proteina s histološkim gradusom tumora, TNM klasifikacijom, lokalizacijom tumora, veličinom, te dobi i spolom bolesnika. Dokazana je izraženost proteina MAGE-A3/4 u 90,1% karcinoma i u 100% metastaza u limfne čvorove, proteina NY-ESO-1 u 96,4 karcinoma i 92,9 % metastaza. Pozitivna reakcija je nađena samo u citoplazmi tumorskih stanica. Ustanovljeno je da izraženost proteina MAGE-A3/4 pozitivno korelira s izraženosti NY-ESO-1 u istom tumor (Pearsonov test korelacije r=0,310), da izraženost proteina NY-ESO-1 u tumoru pozitivno korelira s izraženosti istog u metastazi u limfnom čvoru (Pearsonov koeficijent korelacije r=0,605, Spearmanov faktor korelacije, rho=0,588). Ustanovljeno je da dob negativno korelira s izraženosti proteina NY-ESO-1 u tumoru (x2 test P=0,001, Pearsonov test korelacije r= - 0,516, Spearmanov faktor korelacije, rho= - 0,515). U tijeku su istraživanja imunoterapije vakcinacijom kod bolesnika s melanomom, koji imaju ekspresiju tumorskih antigena, no isto tako napravljeni su i prvi koraci u pokušaju imunoterapije karcinoma pločastih stanica jednjaka. Zbog visoke izraženosti proteina MAGE-A3/4 i NY-ESO-1 u oko 90 % uzoraka karcinoma jednjaka, postavljena je osnova za moguću primjenu imunoterapije. Actions (login required) Downloads Downloads per month over past year