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Scientific paper “Essential oil volatile fingerprint differentiates Croatian cv. Oblica from other Olea europaea L. cultivars”, by the group of authors Marijana Popović, Maja Jukić Špika, Maja Veršić Bratinčević, Tonka Ninčević, Ana Matešković, Marija Mandušić, Jakša Rošin, Marija Nazlić, Valerija Dunkić and Elda Vitanović was accepted and published in the journal Molecules 2021, 26 (2), 3533. The paper is visible on the WoS platform. The mentioned scientific paper is the second scientific paper published within the LoVeFly project so far. Selection of olive groves for field research Posted on 3. srpnja 2021. by Jaksa Rosin Prema planiranim projektnim aktivnostima obavljen je odabir maslinika u kojima će se provoditi daljnja testiranja odabranih hlapivih tvari na privlačenje maslininog moljca maslinin moljac (Prays oleae Bern.) i maslinine muhe (Bactrocera oleae Rossi) . Odabir maslinika je obavljen na način da su prvo izdvojeni maslinici koji svojom veličinom odgovaraju traženim uvjetima. Detaljnijim proučavanjem zemljopisnih položaja maslinika i njihovih karakteristika napravljen je uži odabir. Nakon razgovora s vlasnicima maslinika te spoznajom o načinu korištenja njihovih maslinika odabrana su po dva maslinika za svako uzgojno podpodručje masline u Republici Hrvatskoj (zaobalje, priobalje, otoci); od kojih će se u jednom provoditi testiranje hlapivih tvari na privlačenje maslinog moljca, a u drugom na privlačenje maslinine muhe According to the planned project activities, the selection of olive groves in which further tests of selected volatile substances to attract the olive moth Olive moth (Prays oleae Bern.) and olive fly (Bactrocera oleae Rossi) will be carried out was made. The selection of olive groves was done in such a way that the olive groves were first selected by its size that corresponded to the required conditions. A more detailed study of the geographical locations of olive groves and their characteristics made a shortlist. After talking with the owners of the olive groves and learning about the way of use of their olive groves, two olive groves were selected for each olive growing area in the Republic of Croatia (inland, coast, islands); of which in one case volatile substances will be tested to attract the olive moth and in the other to attract the olive fly. Presentation of the LoVeFly project at the Science Festival 2021. From 10 to 15 May 2021, the Science Festival was held in Split. As part of the activities entitled “Mediterranean Agriculture and Forestry – Research at the Institute for Adriatic Crops and Karst Reclamation”, the Institute’s staff held a virtual workshop on Friday, May 14 from 10 am to 11 am. At the workshop they presented several selected current results, achievements and current projects implemented in the field of agriculture and forestry at the Institute by broadcasting from the laboratories, greenhouses, experimental sites and plantations. Among other activities, Ana Matešković presented the project “New methods in olive pests controlling using plant volatiles”. Lecture at the 23rd international event of olive growers and olive oil producers “Noćnjak 2021.” Organized by the Cooperative Alliance of Dalmatia, the 23rd International event of olive growers and olive oil producers “Noćnjak 2021” was held from 27 to 30 April 2021, this year for epidemic reasons online for the first time. More details on Noćnjak 2021 (in Croatian) As part of the Noćnjak 2021, a scientific-professional meeting was held under the leadership of PhD Mirella Žanetić, associate on the LoVeFly project. As part of meeting, the PhD student, Ana Matešković, M. Sc., had a lecture in Croatian entitled “Mirisne tvari masline koje bi mogle biti odgovorne za privlačenje maslininog moljca i muhe” (in English: “Fragrant substances of olive that could be responsible for attracting olive moth and olive fly”) by the group of authors Ana Matešković, M. Sc.; PhD Marijana Popović; PhD Maja Jukić Špika; PhD Tonka Ninčević; MSc Jakša Rošin; Marija Mandušić, mag.ing.agr.; PhD Maja Veršić Bratinčević, PhD Mirella Žanetić and PhD Elda Vitanović. In presentation she showed the results obtained during the first year of research of the mentioned project to interested olive growers and olive oil producers. Lecture “Fragrant substances of olive that could be responsible for attracting olive moth and olive fly” (in Croatian) Public procurement of the Gas Chromatograph-Mass Spectrometer (GC-MS) Based on the conducted Open Public Procurement Procedure of small value, published on September 21, 2020, the Gas Chromatograph-Mass Spectrometer (GC-MS) model GCMS-QP2020NX with AOC6000 autosampler and required accessories from Shimadzu d.o.o. was selected. According to the signed Public Procurement Agreement, GC-MS was delivered and installed in the Laboratory for Metabolomics of the Institute for Adriatic Crops and Karst Reclamation, and two associates on the project were educated on its use. Lecture at the Institute for Adriatic Crops and Karst Rclamation On Friday, April 9, the lecture “New methods in olive pests controlling using plant volatiles – LoVeFly” was held in the Institute for Adriatic Crops and Karst Reclamation. Project leader PhD Elda Vitanović introduced the work on the project and the obtained results of the first year of research to the participants. The lecture was attended by about 20 participants. Determination of total phenols The determination of total phenolic compounds of olive fruits harvested in three phases of fruit maturity (green, semi-matured and matured fruit) was done at the Institute for Adriatic Crops and Karst Reclamation. According to the planned project activities 27 samples of olive fruits of the cultivars Lastovka, Leccino and Frantoio were analyzed on the High-performance liquid chromatography (HPLC) device. Paper published on Web of Science The scientific paper of the group of authors was published on the Web of Science: Vitanović, E.; Lopez, J.M.; Aldrich, J.R.; Jukić Špika, M.; Boundy-Mills, K.; Zalom, F.G. Yeasts Associated with the Olive Fruit Fly Bactrocera oleae (Rossi) (Diptera: Tephritidae) Lead to New Attractants. Paper is available on: https://www.mdpi.com/2073-4395/10/10/1501 LoVeFly team welcomes new project member! Maja Veršić Bratinčević, PhD is employed as a postdoctoral researcher at the Department of Applied Sciences of the Institute for Adriatic Crops and Karst Reclamation. She graduated at the Faculty of Chemical Technology, University of Split, majoring in Chemistry and Technology of Mediterranean Cultures, with her dissertation entitled “Glucosinolate degradation products and other volatile compounds in endemic plants of the family Brassicaceae: Aurinia Sinuata (L.) GRISEB. and Aurinia Leucedea (GUSS.) C. KOCH”. She defended her doctoral thesis entitled “Addictive substances in biological samples: determination and stability” in 2015 at the Faculty of Chemical Engineering and Technology, University of Zagreb, where she earned her PhD in natural sciences. In 2018, she was elected as a research associate in the interdisciplinary field of science (pharmacy and basic medical sciences). She gained work experience as a research novice in toxicology laboratory at the Clinical Hospital Centre Split, at the Department of Pathology, Cytology and Forensic Medicine, and at the Faculty of Medicine, University of Split, where she was employed until 2018 as a postdoctoral researcher. As an author or co-author, she participated in the publication of eight scientific papers, several conference papers, and chapters of the textbook “Fundamentals of Forensic Toxicology”. She is a mentor or co-mentor on 13 graduate or final theses. She has participated in various scientific conferences, workshops and seminars for professional development. The main scientific interest includes the analysis of samples using instrumental analysis techniques and primarily the analysis of samples using chromatographic techniques. The area of her research will include examining the profiles of volatile substances and their impact on attracting the most significant olive pests. Maja, welcome! Extraction of essential oils of olive leaves harvested in ripe fruit phase Hydrodistillation of dried leaves of selected olive varieties harvested in ripe fruit phase was performed in a Clevenger apparatus (for two hours and 30 minutes) in the laboratory of the Institute for Adriatic Crops and Karst from September 24 to September 27. The essential oils obtained by the hydrodistillation process were dried with anhydrous sodium sulfate. Samples of essential oils prepared in this way are stored in glass bottles at a temperature of 4°C. These essential oils will determine the profile of volatile substances, which could be responsible for attracting the olive fruit fly (Bactrocera oleae Rossi).
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Na Prirodoslovno-matematičkom fakultetu u Splitu obavljene su analize hlapivih spojeva ploda i lista triju sorti maslina u fazi zelenih, polu-zrelih i zrelih plodova te hlapivih spojeva iz eteričnih ulja lista u fazi polu-zrelih i zrelih plodova, a kako slijedi: 1) eterična ulja lišća maslina 2) ultrazvučna ekstrakcija hlapivih spojeva iz plodova i lišća maslina 3) adsorpcija hlapivih spojeva plodova i lišća masline 4) analiza hlapivih spojeva na plinskom kromatografu-spektrometru masa U kolekcijskom nasadu Instituta za jadranske kulture i melioraciju krša u Kaštel Starom obavljeno je uzorkovanje listova masline u svrhu određivanja intenziteta napada filofagne generacije maslininog moljca. Po sorti je ubrano 200 listova koji su pregledani pod lupom kako bi se utvrdila prisutnost ili odsutnost navedenog štetnika. Na Institutu za jadranske kulture i melioraciju krša u Splitu obavljeno je određivanje ukupnih fenolnih spojeva plodova masline, na plodovima masline ubranim u tri faze zrelosti ploda (zeleni, polu-zreli i zreli plodovi). Prema planiranim projektnim aktivnostima na uređaju za tekućinska kromatografiju visoke djelotvornosti (HPLC) analizirano je 27 uzoraka plodova masline sorata Lastovka Leccino i Frantoio. Na platformi Web of Science objavljen je znanstveni rad grupe autora Vitanović, E.; Lopez, J.M.; Aldrich, J.R.; Jukić Špika, M.; Boundy-Mills, K.; Zalom, F.G. Yeasts Associated with the Olive Fruit Fly Bactrocera oleae (Rossi) (Diptera: Tephritidae) Lead to New Attractants. Dr. sc. Maja Veršić Bratinčević zaposlena je kao poslijedoktorand na Institutu za jadranske kulture i melioraciju krša na Zavodu za primijenjene znanosti. Diplomirala je na Kemijsko-tehnološkom fakultetu, Sveučilište u Splitu, na smjeru Kemija i tehnologija mediteranskih kultura, obranivši diplomski rad pod nazivom „Glukozinolatni razgradni produkti i ostali hlapljivi spojevi u endemičnim biljkama porodice Brassicaceae: Aurinia Sinuata (L.) GRISEB. i Aurinia Leucedea (GUSS.) C. KOCH“. Doktorski rad, pod nazivom „Sredstva ovisnosti u biološkim uzorcima: određivanje i stabilnost“, obranila je 2015. godine na Fakultetu kemijskog inženjerstva i tehnologije, Sveučilišta u Zagrebu, čime je stekla zvanje doktora prirodnih znanosti. Godine 2018. izabrana je u znanstveno zvanje- znanstvena suradnica za interdisciplinarno područje znanosti (farmacija i temeljne medicinske znanosti). Radno iskustvo stekla je kao znanstvena novakinja u Kliničkom bolničkom centru Split, na Zavodu za patologiju, citologiju i sudsku medicinu, te na Medicinskom fakultetu, Sveučilište u Splitu, gdje je do 2018. godine bila zaposlena na radnom mjestu poslijedoktoranda. Sudjelovala je, kao član istraživačkog tima, u radu toksikološkog laboratorija, te u izvođenju nastave na četiri sastavnice Sveučilišta u Splitu. Kao autor ili koautor sudjelovala je u objavljivanju osam znanstvenih radova, više kongresnih priopćenja, te poglavlja udžbenika „Osnove forenzične toksikologije“. Mentor ili ko-mentor je na 13 diplomskih ili završnih radova. Sudjelovala je na raznim znanstvenim skupovima, radionicama i seminarima za strukovno usavršavanje. Majin znanstveni interes uključuje analizu uzoraka koristeći instrumentalne tehnike analize, i to prvenstveno kromatografske tehnike analize. Područje njenog znanstveno-istraživačkog rada uključivat će ispitivanje profila hlapivih tvari te njihovog utjecaja na privlačenje najznačajnijih štetnika masline. U laboratoriju Instituta za jadranske kulture i melioraciju krša provedena je od 24. do 27. studenog 2020. godine hidrodestilacija osušenih listova, ubranih u fazi zrelih plodova masline odabranih sorti, u Clevenger aparatu (u trajanju od dva sata i 30 minuta). Eterična ulja dobivena procesom hidrodestilacije osušena su pomoću bezvodnog natrijevog sulfata. Tako pripremljeni uzorci eteričnih ulja čuvaju se u staklenim bočicama na temperaturi od 4°C. Navedenim eteričnim uljima odredit će se profil hlapivih tvari, koje bi mogle biti odgovorne za privlačenje maslinine muhe (Bactrocera oleae Rossi). U laboratoriju Instituta za jadranske kulture i melioraciju krša provedena je od 19. do 28. listopada 2020. godine hidrodestilacija osušenih listova, ubranih u fazi poluzrelih plodova masline odabranih sorti, u Clevenger aparatu (u trajanju od dva sata i 30 minuta). Eterična ulja dobivena procesom hidrodestilacije osušena su pomoću bezvodnog natrijevog sulfata. Tako pripremljeni uzorci eteričnih ulja čuvaju se u staklenim bočicama na temperaturi od 4°C. Navedenim eteričnim uljima odredit će se profil hlapivih tvari, koje bi mogle biti odgovorne za privlačenje maslinine muhe (Bactrocera oleae Rossi). Krajem listopada u pokusnom kolekcijskom masliniku Instituta za jadranske kulture i melioraciju krša, na tri sorte masline, ponovno su uzorkovani plodovi i listovi masline, ovaj put obojani plodovi (zreli plodovi). Na istima će biti napravljene jednake analize kao i prilikom prvog i drugog uzorkovanja: 1. određivanje fizikalnih parametra plodova i listova, 2. određivanje sastava hlapivih tvari plodova i listova, 3. određivanje postotka napada maslinine muhe, 4. određivanje sadržaja fenolnih spojeva plodova i listova, 5. te ekstrakcija eteričnih ulja listova u svrhu određivanja hlapivih tvari eteričnih ulja istih. Sve navedene analize provode se u svrhu izolacije hlapivih tvari, karakterističnih za određenu sortu, koje bi mogle biti odgovorne za privlačenje maslinine muhe. Na Prirodoslovno-matematičkom fakultetu u Splitu započete su analize hlapivih spojeva u malim plodovima veličine 4-5mm i lista triju sorti maslina te hlapivih spojeva iz eteričnih ulja lista u fazi zelenog ploda kako slijedi: 1) eterična ulja lišća maslina 2) ultrazvučna ekstrakcija hlapivih spojeva iz plodova i lišća maslina 3) adsorpcija hlapivih spojeva plodova i lišća masline 4) analiza hlapivih spojeva na plinskom kromatografu-spektrometru masa