http___medlib.mef.hr_179__2.xml
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Mitrečić, Dinko and Mavrić, Sandra and Gajović, Srećko (2008) PCR-based identification of short deletion/insertions and single nucleotide substitutions in genotyping of splotch (Pax3(sp)) and truncate (Noto(tc)) mouse mutants. Molecular and cellular probes, 22 (2). Abstract Splotch (Pax3(sp)) and truncate (Noto(tc)) are spontaneously arisen mouse mutants with disturbed embryo development. Splotch carries a Pax3 mutation and it is characterized by the neural tube defect. Corresponding mutation in human causes Waardenburg syndrome. Truncate is Noto mutant with disturbed development of the caudal notochord. In order to establish easy genotyping procedure of these mutations, it was tested whether simple PCRs with single primer pairs could be used for this purpose. As it was necessary to differentiate sequence variants on the scale of one to several nucleotides, the approach referred to as "3' variable primer ends" was applied. The method was based on the presence of discriminating nucleotides at the 3' end of the primer sequence. This approach was successfully applied in genotyping adult mice and embryos of splotch with a 6bp deletion/insertion and truncate with a single nucleotide substitution. Described genotyping approach facilitates recognizing of these mutations and it could be in general used for detection of sequence differences in one to several nucleotides. Actions (login required) Downloads Downloads per month over past year
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Mitrečić, Dinko (2006) Razvoj kaudalnog dijela zametka u mišjih mutanata splotch i truncate [ Development of the caudal part of splotch and truncate mouse embryos ]. PhD thesis, Sveučilište u Zagrebu. Abstract Development of the caudal part of vertebrate embryo occurs when gastrulation is completed and after the regression of the primitive streak. Tail structures form directly from the tail bud blastema, by secondary body development. The caudal part of the body is malformed in splotch and truncate embryos. Splotch is caused by mutation of Pax3 gene and it is characterized by the open neural tube (spina bifida) and defective neural crest migration. Truncate is mutant of Noto gene, characterized by defective development of the notochord. The hypothesis of this study was that the phenotype of splotch and truncate mutants resulted from defective tail bud and secondary body development. The aim of this work was to describe development of the caudal part of C57Bl/6 embryos and to compare it to those of splotch and truncate. Morphology of 9-12.5 days old embryos was investigated by light microscopy using semithin toluidin stained histology sections. The secondary body development in the caudal part of the mouse embryo included gradual differentiation of the tail bud. Tail bud divided and formed the medullary cord and the tail cord. The medullary cord differentiated in the neural tube and the tail cord differentiated in the notochord and the tail gut. Defective neurulation in splotch embryos was not accompanied by defective tail bud morphology, except in 12.5 days old embryos, when complete morphology of the tail, caudal from spina bifida, was disturbed. Spina bifida in splotch embryos developed at the primary and secondary neural tube boundary. In truncate embryos notochord was partially missing. Differentiation of the tail bud was disturbed and the notochord did not develop from the tail cord, but from ventral neuroectoderm. Dorsal part of the tail cord, instead of the notochord, formed additional tail gut. Lack of the notochord was accompanied by disturbed development of the neighboring structures, the neural tube and the somites. Described morphogenetic processes which lead to secondary neural tube and notochord malformations contribute to understanding of human birth defects – spina bifida, caudal disgenesis syndrome and Waardenburg syndrome. Abstract in Croatian Razvoj kaudalnog dijela zametka kralješnjaka odvija se nakon završetka gastrulacije i nestanka primitivne pruge sekundarnim mehanizmom razvoja, tijekom kojeg strukture osnove repa nastaju od nediferenciranih stanica repnog pupoljka. Kaudalni dio zametka poremećen je u mišjih mutanata splotch i truncate. U splotch miševa mutiran je gen Pax3, što ima za posljedicu otvorenu neuralnu cijev (spina bifida) i poremećaj migracije stanica neuralnog grebena. U truncate miševa mutiran je gen Noto, gdje je poremećen razvoj notokorda. Hipoteza ovog istraživanja bila je da je fenotip mutiranih miševa splotch i truncate posljedica nepravilnog razvoja repnog pupoljka, te poremećenog sekundarnog mehanizma razvoja. Cilj rada bio je usporediti razvoj kaudalnog dijela zametka visoko srođenog soja C57Bl/6 s poremećenim razvojem mutanata splotch i truncate. Građa mišjih zametaka starih od 9 do 12,5 dana proučavana je svjetlosnom mikroskopijom serijskih polutankih rezova obojanih toluidinskim modrilom. U zamecima C57Bl/6 miševa sekundarni mehanizam razvoja obuhvaća postupnu diferencijaciju stanica repnog pupoljka, koje se dijele na moždinski tračak od kojeg nastaje sekundarna neuralna cijev, i repni tračak od kojeg nastaje notokord i repno crijevo. Poremećena neurulacija u splotch zametaka nije praćena poremećajem građe repnog pupoljka, osim u splotch zametaka starih 12,5 dana, gdje dolazi do sveobuhvatnog poremećaja razvoja osnove repa kaudalno od spine bifide. Spina bifida u splotch zametaka nastaje na prijelazu iz primarne u sekundarnu neuralnu cijev. U truncate zametaka djelomično nedostaje notokord. Diferencijacija repnog pupoljka je poremećena, te se notokord umjesto od repnog tračka odvaja od ventralne strane sekundarne neuralne cijevi. Dorzalni dio repnog tračka umjesto notokorda oblikuje dodatni lumen repnog crijeva. Nedostatak notokorda u osnovi repa povezan je s poremećajem razvoja susjednih struktura, neuralne cijevi i somita. Opisana morfogenetska zbivanja doprinose razumijevanju razvojnih poremećaja u čovjeka - spine bifide, sindroma kaudalne disgeneze i Waardenburgovog sindroma. Actions (login required) Downloads Downloads per month over past year