http___medlib.mef.hr_571__2.xml
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Murat-Sušić, Slobodna and Lipozenčić, Jasna and Žižić, Vesna and Husar, Karmela and Marinović, Branka (2006) Serum eosinophil cationic protein in children with atopic dermatitis. International Journal of Dermatology, 45 (10). Abstract BACKGROUND: Eosinophil cationic protein (ECP) is a cytotoxic agent secreted by activated eosinophils during allergic and inflammatory processes. The aim of the study was to determine the ECP level, absolute and relative eosinophil count and IgE antibodies in children with atopic dermatitis (AD) compared with those of nonatopic children, and to assess the correlation of these laboratory parameters with the clinical severity of AD. ----- METHODS: This prospective study comprised 70 children. There were 49 children with AD aged 3-36 months, and the control group comprised 21 children with a negative personal and family history for atopic diseases. Detailed history, serum ECP levels (UniCAP FEIA), relative and absolute eosinophil counts and total serum IgE antibodies were determined in both groups. In the children with AD, skin involvement was measured by the SCORAD index. ----- RESULTS: The calculated SCORAD index was between 16 and 83. IgE antibodies, relative and absolute eosinophil counts showed a significantly wider range of values and a statistically higher median (P   0.05). The same was found for the correlation of serum ECP and intensity of skin changes (r = -0.095) and serum ECP and subjective symptoms (r = -0.045). The correlation was positive, but weak and statistically not significant for the serum ECP and extent of the skin lesions (r = 0.079, P > 0.05). ----- CONCLUSION: Elevated levels of ECP, relative and absolute eosinophil counts, as well as IgE antibodies were determined in the patients with AD. As these laboratory findings did not correlate with the severity of AD, they can be considered only as additional methods in the evaluation of patients with AD. Actions (login required) Downloads Downloads per month over past year
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Vagić, Davor (2008) Odnos upalne stanične infiltracije i biljega aktivacije upalnih stanica u bolesnika s kroničnim čeljusnim rinosinuitisom [Relation between cellular infiltration and markers in patients with chronic rhinosinusitis]. PhD thesis, Sveučilište u Zagrebu. Abstract OBJECTIVES: The aim of the study was to show the difference in the pattern of inflammation (and Th1/Th2 polarization) between asthmatic and non-asthmatic patients with CRS, specifically eosinophil activation, local IgE levels in the sinus fluid and tissue, and the severity of inflammation were measured. ----- METHODS: The maxillary sinus lavages, mucosal biopsies and bacteriological swabs were taken in 23 asthmatic and 36 non-asthmatic adult patients with CRS. The concentrations of IgE, eosinophil cationic protein (ECP), myeloperoxidase (MPO), and tryptase were analyzed and IgE+ cells, eosinophils, lymphocytes and plasma cells counted. ----- RESULTS: The granulocyte activation markers and IgE in sinus lavages, and the inflammatory and IgE + cells counts were significantly higher in the asthmatics with the greatest difference in ECP and IgE concentrations. The tryptase concentrations did not differ, but only in the asthmatics they correlated significantly with the IgE concentrations and IgE+ cells count. Subjective scores were decreased after therapy in both groups of patients and without correlation with objective parameters of inflammation. Conclusion. Asthmatic patients present a distinct subgroup among the patients with chronic rhino sinusitis (CRS). The levels of the cellular markers and Age in the sinus fluid differ from those of non-asthmatic patients with CRS. The activation of granulocytes (especially eosinophils), local IgE concentrations and the inflammatory cells infiltration are significantly higher in the asthmatics. Abstract in Croatian Istraživanje je proučavalo različite putove upalne reakcije kod ispitanika s kroničnim rinosinuitisom s i bez astme. U istraživanje je bilo uključeno 36 ispitanika s kroničnim rinosinuitisom i 23 ispitanika s kroničnim rinosinuitisom i astmom. Kod svih ispitanika učinjena je sinusoskopija čeljusnih sinusa, ispirak sinusa, biopsija sluznice i mikrobiološki bris. Iz ispirka se određivala koncentracija eozinofilnog kationskog proteina (ECP), mijeloperoksidaze (MPO), triptaze i IgE protutijela. Iz bioptata sluznice brojali su se IgE pozitivne stanice, eozinofili, plazma stanice i limfociti. Upitnik simptoma kroničnog rinosinuitisa ispitanici su ispunjavali na početku istraživanja i nakon 6 mjeseci. ECP, MPO, koncentracija IgE protutijela u ispirku, broj upalnih stanica i IgE pozitivnih stanica značajno su viši u astmatičara. Koncentracija triptaze povezana je samo u astmatičara s koncentracijom IgE protutijela i brojem IgE pozitivnih stanica. U 25 ispitanika s kroničnim rinosinuitisom izolirana je bakterija u sinusu dok je u astmatičara izolirana u 16 ispitanika. Značajna je povezanost ECP-a i MPO-a u bakteriološki pozitivnim sinusima astmatičara. U obje grupe ispitanika zbroj subjektivnih simptoma značajno se smanjuje nakon terapije bez korelacije s objektivnim prametrima upale. Sukladno navedenim rezultatima zaključak je da grupa astmatičara predstavlja posebnu grupu među ispitanicima s kroničnim rinosinuitisom. Actions (login required) Downloads Downloads per month over past year