http___medlib.mef.hr_347__2.xml
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Židovec Lepej, Snježana and Tešović, Goran and Ljubin Sternak, Sunčanica and Jeren, Tatjana (2007) Naive and memory CD4+ T-cells in the cerebrospinal fluid of children with aseptic meningitis following measles-mumps-rubella vaccination and enteroviral meningitis. Immunological investigations, 36 (3). English abstract We investigated the distribution of memory (CD45RO+) and naive (CD45RA+CD62L+) CD4+ T-cells as well as CD8+ T-cells and total T-cells in the CSF of children with aseptic meningitis following measles-mumps-rubella (MMW) vaccination and those with enteroviral meningitis. Flow cytometric analysis of CSF cells was performed in 12 children with MMR vaccine-associated meningitis and 11 children with enteroviral meningitis. Percentages of total T-cells, CD4+ and CD8+ T-cells and monocytes in CSF of patients from the two groups were not significantly different. The majority of CD4+ T-cells in the CSF of both patient groups were of memory phenotype. Percentages of CSF naive CD4+ T-cells were increased in children with aseptic meningitis following MMR vaccination. Further studies focused on the more detailed immunophenotyping of CSF cells are needed to fully establish the usefulness of flow cytometry in the diagnostic workup of inflammatory CNS diseases in children. Actions (login required)
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Boras, Zagorka (2004) Citokinski profil limfocita T iz krvi i bronhoalveolarnog ispirka u bolesnika s plućnom tuberkulozom [ Cytokine profile of T-lymphocytes from peripheral blood and bronchoalveolar lavage fluid in patients with active pulmonary tuberculosis ]. PhD thesis, Sveučilište u Zagrebu. Abstract Cell-mediated immunity, especially from CD4+ T-lymphocyte function, plays an important role in eliminatig mycobacteria in tuberculosis. Reports on Th1-type and Th2-type cytokines in human tuberculosis are conflicting, and little is known about their role in tissue damage. We examined the cytokine responses of CD4+ and CD8+ T-lymphocytes in peripheral blood and bronchoalveolar lavage fluid (BAL) from 20 purified protein derivative skin-positive (PPD+) and from nine PPD skin-negative patients with active pulmonary tuberculosis. Parallel blood and BAL fluid samples from each subject were shortly incubated with or without PMA and ionomycin, and the proportions of CD4+ and CD8+ T-lymphocytes producing interferon- (IFN-) or interleukin-4 (IL-4) were measured by flow cytometry. There was no age or BMI difference of examiness between PPD+ and PPD- group. PPD- patients, in relation to PPD+ patients, had a significant higher percent of CD4+ T-lymphocytes in the BAL. No significant difference was found between groups in percent CD4+ T-lymphocytes in peripheral blood and CD8+ T-lyphocytes peripheral blood and BAL. At the same time, PPD- patients have shown a significant higher percent of CD4+ T-lymphocytes which produced IFN- in relation to the entire number activated CD4+ T-lymphocytes in a peripheral blood. No significant difference was found in the production IFN- of CD8+ T-lymphocytes peripheral blood and BAL and CD4+ T-lymphocytes in the BAL. CD4+ T-lymphocytes of peripheral blood in two PPD-patients produced IL-4 while the rest of the T-lymphocytes peripheral blood and BAL suppressed the production of this cytokine. Among PPD+ patients group we have found a higher percent of CD4+ as well as CD8+ T-lymphocytes which produce IFN- in BAL in relation to the peripheral blood, while the BAL in PPD- group was more active only in CD4+ production IFN-. These results suggest a higher immune activity in the blood of PPD- patients while the BAL is more active in both groups of examinees in relation to the peripheral blood. It suggests that the separation of T-lymphocytes, specially those producing IFN- from peripheral blood to the lungs, occurs in tuberculosis. Abstract in Croatian Stanicama je posredovana imunost, naročito CD4+ T-limfocitima, ključna u eliminaciji mikobakterija u tuberkulozi. Dosadašnja saznanja o Th1 i Th2 tipu citokinske proizvodnje u ljudskoj tuberkulozi konfliktna su i malo se zna o njihovoj ulozi u tkivnom oštećenju. Mi smo istraživali citokinski odgovor CD4+ i CD8+ T-limfocita periferne krvi i BAL-a u 20 PPD+ i devet PPD- bolesnika s aktivnom plućnom tuberkulozom. Uzorci periferne krvi i BAL-a svakog bolesnika bili su kratkotrajno inkubirani s PMA i ionomicinom ili bez njih, nakon čega smo na protočnom citometru određivali CD4+ i CD8+ T-limfocite koji su proizvodili IFN- ili IL-4. Između PPD+ i PPD- skupine nije bilo razlike u dobi ispitanika i BMI. PPD- bolesnici su, u odnosu na PPD +, imali značajno veći postotak CD4+ T-limfocita u BAL-u. Nije bilo značajnije razlike među grupama u postotku CD4 T-limfocita u perifernoj krvi i CD8+ u perifernoj krvi i BAL-u. Istovremeno, PPD- bolesnici iskazali su značajno veći postotak CD4+ T-limfocita u perifernoj krvi koji su proizvodili IFN-, a u odnosu na sveukupni broj tih aktiviranih CD4+ T-limfocita. Nije bilo razlike između skupina u proizvodnji IFN- od CD8+ T-limfocita krvi i BAL-a i CD4+ T-limfocita BAL-a. CD4+ T-limfociti periferne krvi dva PPD- bolesnika su proizvodili IL-4, a ostali su T-limfociti periferne krvi i BAL-a zakazali u proizvodnji navedenog citokina. Unutar PPD+ skupine bolesnika našli smo značajno veći postotak i CD4+ i CD8+ T-limfocita koji proizvode IFN- u BAL-u u odnosu na perifernu krv, dok je u PPD- skupine BAL bio aktivniji samo u CD4+ proizvodnji IFN-. Ovakvi rezultati sugeriraju veću imunološku aktivnost u PPD- bolesnika u krvi, a BAL je obje skupine ispitanika aktivniji u odnosu na perifernu krv. Sve to sugerira da u tuberkulozi dolazi do odjeljivanja T-limfocita, naročito onih koji proizvode IFN-, iz periferne krvi u pluća. Actions (login required) Downloads Downloads per month over past year