http___medlib.mef.hr_1645__1.xml
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Salopek, Daniela and Grčević, Danka and Katavić, Vedran and Kovačić, Nataša and Lukić, Ivan Krešimir and Marušić, Ana (2008) Increased bone resorption and osteopenia are a part of the lymphoproliferative phenotype of mice with systemic over-expression of interleukin-7 gene driven by MHC class II promoter. Immunology Letters, 121 (2). English abstract Mice with interleukin (IL)-7 transgene under the control of E(alpha) promoter over-express IL-7 in MHC class II-positive cells and develop specific immune phenotype, marked by an increase in CD45R(+) cells in both the bone marrow and peripheral blood. We show that IL-7 transgenic mice have a bone phenotype characterized by an age-related loss of trabecular bone in both axial and long bones. Osteopenia was the result of increased number of active osteoclasts on the surface of trabecular bone. Furthermore, IL-7 transgenic mice showed increased osteoclastic but unchanged osteoblastic potential of the bone marrow in vitro. IL-7 over-expression also created osteoclastogenic microenvironment within the bone marrow which promoted the commitment of precursors towards the osteoclast lineage. These findings are important for immunological disturbances where IL-7 is involved and where alterations in the immune system are accompanied by changes in bone metabolism, such as multiple myeloma, rheumatoid arthritis and postmenopausal osteoporosis. Actions (login required)
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Žitnjak, Daniela (2009) Koštani fenotip miševa s transgenom za interleukin 7 (IL-7) [Bone phenotype of IL-7 transgenic mice]. PhD thesis, Sveučilište u Zagrebu. Croatian abstract Miševi s transgenom za IL-7 koji je pod kontrolom Ea promotora pojačano izražavaju IL-7 u svim stanicama koje na staničnoj membrane nose MHC II antigen te razvijaju karakterističan imunološki fenotip koji obilježava porast udjela stanica pozitivnih za CD45R antigen u koštanoj srži i u perifernoj krvi. Ženke tih miševa pokazuju i koštani fenotip obilježen gubitkom trabekularne kosti u aksijalnim i dugim kostima koji se razvija tijekom njihovog starenja. Osteopenija je posljedica povećanog broja aktivnih osteoklasta na površini trabekularne kosti. Nadalje, u kulturama neadheriranih i CD45R+ stanica koštane srži transgeničnih miševa nisu uočene promjene u broju osteoblasta, unatoč povećanom osteoklastogeničnom potencijalu koštane srži. Povećani izražaj IL-7 dovodi do promjena u mikrookolišu unutar koštane srži čime su stanice preteče usmjerene prema osteoklastnoj lozi. English abstract Mice with an interleukin (IL)-7 transgene under the control of Ea promoter overexpress IL-7 in MHC class II-positive cells and develop specific immune phenotype, marked by an increase in CD45R+ cells in both the bone marrow and peripheral blood. We show that female IL-7 transgenic mice have a bone phenotype characterized by an age-related loss of trabecular bone in both axial and long bones. Osteopenia was the result of increased number of active osteoclasts on the surface of trabecular bone. Furthermore, IL-7 transgenic mice showed increased osteoclastic but unchanged osteoblastic potential of the bone marrow in vitro, both from nonatherent bone marrow cells and CD45R+ marrow cells. IL-7 over-expression also created osteoclastogenic microenvironment within the bone marrow which promoted the commitment of precursors towards the osteoclast lineage. Actions (login required)