http___www.irb.hr_Istrazivanja_Zavodi_Zavod-za-molekularnu-medicinu_Laboratorij-za-oksidacijski-stres_1.xml
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BIOXYARN – In vitro evaluation of the biocompatibility of nanofibrous yarns from an oxidative stress perspective Fibres and textiles have been used as biomaterials for thousands of years, mainly as sutures and in dressings for wound care. Recently, it has become of increasing interest to use fibres as implantable materials to support the repair of damaged tissues and organs. In particular, the emergence of nanoscale electrospun fibres has allowed the fabrication of scaffolds that mimic the architecture of native biological tissues. Many studies have demonstrated that these biomimetic nanofibrous materials have the ability to promote cell adhesion, proliferation, and differentiation. However, mixed results have been observed in vivo with regards to their biological effects, including the inflammatory response. Therefore, there is a strong need to understand better the mechanisms involved in the cell response to nanofibres and to come up with better in vitro models for predicting the biocompatibility of electrospun materials in vivo. In tissue healing processes, oxidative stress has been identified as one of the key pathophysiological elements. Therefore, this project will evaluate the biocompatibility of electrospun yarns, a new and promising generation of electrospun materials, from an oxidative stress perspective. Human fibroblasts will be grown on the materials under standard and induced oxidative stress conditions. The cell response to the materials will be assessed, in particular in respect to the induction of 4-hydroxynonenal, a major bioactive marker of lipid peroxidation known as the "second messenger of free radicals". Particular attention will be given to the effects of material degradation and of added antioxidants on the onset of oxidative stress. This interdisciplinary project will contribute to understand the mechanisms underlying interactions between cells and nanofibres that occur upon implantation. Additionally, it will guide the development of electrospun yarns with improved biocompatibility and will aid to evaluate the risks associated to their implantation. Partner organisations include the University of Oxford (NDORMS) and the University of Zagreb (TTF). - Glavni istraživač / voditelj - Ostali suradnici Prof. Andrew Carr (University of Oxford, NDORMS) Prof. Maja Somogyi Škoc (University of Zagreb, TTF) LabOS members: Dr. Lidija Milković, Mrs. Tea Kečkeš
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Laboratorij za oksidacijski stres Glavna područja istraživanja: - Izučavanje fiziologije i patologije oksidacijskog stresa - Molekularne značajke regulacije rasta putem 4-hidroksinonenala (HNE) - Eksperimentalna dijagnostika i terapija zloćudnih bolesti temeljenih na oksidacijskom stresu - Bioterapija raka - Uzgoj kultura tkiva kosti Laboratorij za oksidacijski stres bavi se izučavanjem fiziologije i patologije oksidacijskog stresa. Oksidacijski stres se najčešće definira kao poremećaj ravnoteže prooksidansa (npr. slobodnih radikala, reaktivnih kisikovih spojeva – ROS) i antioksidansa (vitamini C, E i antioksidacijski enzimi) u korist prooksidansa. Do pomaka ravnoteže može doći ukoliko je smanjena antioksidativna zaštita organizma ili ako je stvaranje radikala pojačano. U stanjima oksidacijskog stresa dolazi do porasta ROS-a u organizmu ili tkivima, te dolazi do oštećenja DNK, proteina i lipida stanične membrane. Oštećenje DNK uzrokuje mutacije, oksidacijom proteini gube svoju funkciju a lipidi podliježu lipidnoj peroksidaciji čiji su konačni produkti reaktivni aldehidi. U istraživanju učinaka oksidacijskog stresa koriste se različitiin vitroiin vivomodeli (kulture animalnih stanica, kvasac, alge, primarnih kultura i rad sa laboratorijskim životinjama). Nadalje rad Laboratorija za oksidacijski stres uključuje eksperimentalnu dijagnostiku, te terapiju zloćudnih bolesti temeljenih na oksidacijskom stresu. Također proučavaju se učinci kirurškog i oksidacijskog stresa u karcinomu kolona, oksidativni stres središnjeg živčanog sustava, patomorfološke značajke oksidativnog stresa u regeneraciji jetre te primarna perkutana koronarna angioplastika. Ana Čipak Gašparović dr.sc. Lidija Milković dr.sc. Neven Žarković Pierre-Alexis Mouthuy DPhil Suzana Borović Šunjić dr.sc. BIOXYARN – In vitro evaluation of the biocompatibility of nanofibrous yarns from an oxidative stress perspective Fibres and textiles have been used as biomaterials for thousands of years, mainly as sutures and in dressings for wound care. Recently, it has become of increasing interest to use fibres as implantable materials to support the repair of damaged tissues and organs.više »