http___www.irb.hr_Ljudi_Iva-Tolic_1.xml
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Iva Tolić Prof. dr. sc. Radionice 2 Ruđer Bošković Institute Bijenička cesta 54 10000 Zagreb Croatia - Education - Projects - Awards and Achievements - Classes - Featured Publications - Membership in professional associations / societies - Miscellaneous Education - 2002: PhD in biology, University of Zagreb, Croatia - 1999 – 2001: PhD work with Prof. Ning Wang, Harvard School of Public Health, Boston, MA, USA - 1997 – 1999: Graduate studies in molecular biology, Research assistant in the group of Prof. Nenad Trinajstić, Ruđer Bošković Institute and University of Zagreb, Croatia - 1992 – 1996: Diploma in molecular biology, University of Zagreb, Croatia Projects - 2015 – 2020: ERC Consolidator Grant. Topic: A new class of microtubules in the spindle exerting forces on kinetochores. Total amount granted: 2,150,000 EUR. - 2015 – 2019: Research grant from the Croatian Science Foundation (HRZZ). Topic: Oscillatory dynamics of the cytoskeleton. Total amount granted: 1,000,000 HRK (≈130,000 EUR). - 2015–2016: Grant from the European Social Fund (ESF), together with Igor Weber (PI), Ruđer Bošković Institute. Topic: Cytoskeletal dynamics and spindle assembly during cell division. Total amount granted: 1,600,000 HRK (≈210,000 EUR). - 2013 – 2016: Research grant from the German Research Foundation (DFG) together with Nenad Pavin, University of Zagreb. Topic: Kinetochore oscillations in mitotic metaphase. Total amount granted: 255,950 EUR. - 2013 – 2015: Research grant from the Unity Through Knowledge Fund (UKF) together with Nenad Pavin (PI), University of Zagreb. Topic: The role of microtubule pivoting in formation of complex structures such as microtubule bundles and mitotic spindles. Total cost of the project: 1,110,000 HRK (≈150,000 EUR). - 2012 – 2015: Research grant from DFG under the Priority Programme SPP 1384: Mechanisms of Genome Haploidization. Topic: Homologous chromosome pairing in Schizosaccharomyces pombe. Total amount granted: 304,400 EUR. - 2010 – 2013: Research grant from the Human Frontier Science Program (HFSP), with Juraj Gregan (PI, University of Vienna) and Daniela Cimini (Virginia Tech). Topic: Molecular architecture and mechanical properties of the kinetochore: a biophysical approach. Total amount granted: 1,000,000 $. - 2009 – 2012: Research grant from DFG under the Priority Programme SPP 1384: Mechanisms of Genome Haploidization. Topic: Homologous chromosome pairing in Schizosaccharomyces pombe. Total amount granted: 252,600 EUR. - 2009 – 2012: Research grant from DFG together with Stefan Diez, MPI-CBG. Topic: In vitro reconstitution of motor-driven nuclear oscillations. Total amount granted: 304,800 EUR. - 2008 – 2012: Research grant from DFG. Topic: Meiotic nuclear oscillations in Schizosaccharomyces pombe. Total amount granted: 246,600 EUR. - 2007 – 2011: Research grant from DFG. Topic: Mechanisms of mitotic spindle alignment by cytoplasmic microtubules and cell shape in Schizosaccharomyces pombe. Total amount granted: 242,600 EUR. Awards and Achievements - EMBO member, since 2018 - Ignaz L. Lieben Award of the Austrian Academy of Sciences, 2017 - Award "Didak" for science, 2017 - National Science Award of the Republic of Croatia, 2016 - Croatian Women of Influence Award, 2016 - Special award of the Ruđer Bošković Institute for outstanding contribution to the scientific excellence and international recognition of the institute, 2015 - European Biophysical Societies Association EBSA Young Investigators' Medal and Prize, 2015 - 5000th ERC grantee, 2015 - MRAK award for creativity in science awarded for the collaboration with the Ruđer Bošković Institute - Order of Danica Hrvatska for science, outstanding contributions to science and its promotion in the Republic of Croatia and the world, 2014 - European Life Science Award in the category Investigator of the Year, 2014 - Chosen by the journal Cell as one of 40 scientists from around the world and working in diverse biological fields, "40 under 40", 2014 - 2001-2002: NATO stipend, Danish Natural Science Research Council Classes - PhD Program in Biophysics at the University of Split, Croatia - Molecular Cytogenetics at the Faculty of Science, University of Zagreb, guest lecturer - Biophysics of the Cell at the Faculty of Science, University of Zagreb, guest lecturer - Biophysics of the Cell Practical at the Faculty of Science, University of Zagreb Featured Publications - Vukušić, K., R. Buđa, A. Bosilj, A. Milas, N. Pavin, I.M. Tolić. (2017) Microtubule sliding within the bridging fiber pushes kinetochore fibers apart to segregate chromosomes. Download PDF - Polak, B., P. Risteski, S. Lesjak, I.M. Tolić. (2017) PRC1-labeled microtubule bundles and kinetochore pairs show one-to-one association in metaphase. Download PDF - Kajtez, J., A. Solomatina, M. Novak, B. Polak, K. Vukušić, J. Rüdiger, G. Cojoc, A. Milas, I. Šumanovac Šestak, P. Risteski, F. Tavano, A.H. Klemm, E. Roscioli, J. Welburn, D. Cimini, M. Glunčić, N. Pavin, I.M. Tolić. (2016) Overlap microtubules link sister k-fibers and balance the forces on bioriented kinetochores. Nat. Commun. Download PDF - Cojoc, G., E. Roscioli, L. Zhang, A. García-Ulloa, J.V. Shah, M.W. Bern, N. Pavin, D. Cimini, I.M. Tolić, and J. Gregan, Laser microsurgery reveals conserved viscoelastic behavior of the kinetochore. Download PDF - Chacon M.R., P. Delivani, I.M. Tolić. Meiotic nuclear oscillations are necessary to avoid excessive chromosome associations. Download PDF - Coelho, M., S.J. Lade, S. Alberti, T. Gross, I.M. Tolić. (2014) Fusion of protein aggregates facilitates asymmetric damage segregation. PLOS Biol. Download PDF - Coelho, M., A. Dereli, A. Haese, S. Kühn, L. Malinovska, M.E. DeSantis, J. Shorter, S. Alberti, T. Gross, I.M. Tolić-Nørrelykke. (2013) Fission yeast does not age under favorable conditions, but does so after stress. Curr. Biol. (Highlighted in Science Editors' Choice; Recommended by Faculty of 1000) Download PDF - Ananthanarayanan, V., M. Schattat, S.K. Vogel, A. Krull, N. Pavin, I.M. Tolić-Nørrelykke. (2013) Dynein motion switches from diffusive to directed upon cortical anchoring. Download PDF - Kalinina, I., A. Nandi, P. Delivani, M.R. Chacón, A.H. Klemm, D. Ramunno-Johnson, A. Krull, B. Lindner, N. Pavin, I.M. Tolić-Nørrelykke. (2013) Pivoting of microtubules around the spindle pole accelerates kinetochore capture. Nat. Cell Biol. Download PDF - Stiess, M., N. Maghelli, L.C. Kapitein, S. Gomis-Rüth, M. Wilsch-Bräuninger, C.C. Hoogenraad, I.M. Tolić-Nørrelykke, F. Bradke. (2010) Axon extension occurs independently of centrosomal microtubule nucleation. (Recommended by Faculty of 1000) Download PDF - Total number of papers in peer-reviewed journals: 75 - Total number of citations: 4700 (from Google Scholar) - h-index: 31 (from Google Scholar) Membership in professional associations / societies - Croatian Biophysical Society - Croatian Society of Biochemistry and Molecular Biology - Croatian Microscopy Society Miscellaneous Scientific career: - since 2014: Senior Research Group Leader and Full Professor, Ruđer Bošković Institute, Zagreb - 2010 – 2014: Senior Research Group Leader (W2, equivalent to Associate Professor), Max Planck Institute of Molecular Cell Biology and Genetics, Dresden - 2005 – 2009: Research Group Leader (W1, equivalent to Assistant Professor), Max Planck Institute of Molecular Cell Biology and Genetics, Dresden - 2003 – 2004: Post-doctoral research with Prof. Francesco Pavone, LENS - European Laboratory for Non-Linear Spectroscopy, Florence, Italy - 2001 – 2002: Post-doctoral research with Prof. Kirstine Berg-Sørensen, Niels Bohr Institute, Copenhagen, Denmark Recent discoveries: - By combining laser ablation, photoactivation, and theory, we have shown that sliding apart of microtubules within the bridging fiber pushes the attached kinetochore fibers and their kinetochores apart, being able to segregate chromosomes independently of the spindle pole (Vukušić, Buđa et al., Dev Cell 2017). - We have discovered that non-kinetochore microtubules, which we term bridging fibers, balance the tension between sister kinetochores by bridging sister k-fibers (Kajtez, Solomatina, Novak et al., Nat Commun 2016). This study challenges the paradigm that the tension on kinetochores is generated by molecular events occurring only at the ends of the k-fibers. - By establishing single-molecule observation and quantification of the motor protein dynein in living cells, we have discovered a new mechanism for how dynein targets cortical anchor sites, by diffusing along the microtubule (Ananthanarayanan et al., Cell 2013). - We have discovered a new mechanism of kinetochore capture during mitosis, based on the pivoting motion of microtubules around the spindle pole (Kalinina et al., Nat. Cell Biol. This concept challenges the current ‘search-and-capture’ paradigm introduced in the 1980s, based on direct growth of microtubules towards kinetochores. - We have demonstrated that fission yeast does not undergo replicative aging, which challenges the dogma that all organisms, including microorganisms, are susceptible to aging (Coelho et al., Curr. Biol. 2013; Coelho et al., PLOS Biol. Mentoring: - 7 PhD, 4 Master and 8 Diploma theses completed. - PhD students have published first-author papers in top journals (Cell, Nature Cell Biology, Developmental Cell, PLoS Biology, Current Biology, EMBO Reports). Current positions of the former PhD students are the following: Vaishnavi Ananthanarayanan is an assistant professor at the Indian Institute of Science; Sven Vogel is a postdoc at the Max Planck Institute of Biochemistry; Miguel Coelho is a HFSP Long-term Fellow at Harvard; Iana Kalinina is a postdoc at the University of Freiburg; Davide Accardi is the leader of the Bioimaging Platform at Champalimaud Foundation in Lisbon; Manuel Neetz is a Patent Professional at Siemens. Invited talks: More than 70 invited talks at conferences and workshops including: Biophysical Society 62nd Annual Meeting, San Francisco, CA, USA, 2018 Gordon Research Conference (GRC) on Motile and Contractile Systems, New London, NH, USA, 2017 EMBO Conference on Meiosis, Oxford, UK, 2015 12th Multinational Congress on Microscopy, Eger, Hungary, 2015 (plenary talk) 10th European Biophysics Congress EBSA, Dresden, Germany, 2015 (plenary talk) The American Society for Cell Biology (ASCB) Annual Meeting, Philadelphia, PA, USA, 2014 Biophysical Society 56th Annual Meeting, San Diego, CA, USA, 2012 Gordon Research Conference (GRC) on Plant and Microbial Cytoskeleton, Andover, NH, USA, 2012 More than 40 invited seminars including: Max Planck Institute (MPI) for Developmental Biology, Tübingen, Germany, 2018 University of Geneva, Switzerland, 2018 Institut Curie, Paris, France, 2018 ETH Zürich, Switzerland, 2015 Engineering and Physical Biology Symposium, Harvard, Cambridge, MA, USA, 2012 California Institute of Technology, Pasadena, CA, USA, 2010 Organization of scientific meetings: - Co-chair at the 2nd ASCB│EMBO Meeting, San Diego, CA, USA, 2018 - Co-chair at the 62nd Annual Meeting of the Biophysical Society, San Francisco, CA, USA, 2018 - Organizer of the EMBO Conference on Meiosis, Hvar, Croatia, 2017 - Co-organizer of ELMI - 17th European Light Microscopy Initiative meeting, Dubrovnik, Croatia, 2017 - Member of the Program Committee for the 9th International Fission Yeast Meeting, Banff, Alberta, Canada, 2017 - Member of the International Advisory Committee for the conference From Solid State to Biophysics VIII, Cavtat, Croatia, 2016 - Member of the Organizing Committee for the EMBO Conference on Meiosis, Oxford, UK, 2015 - Chairperson of the symposium Molecular Motors at the 10th European Biophysics Congress, EBSA 2015, Dresden, Germany, 2015 - Member of the Program Committee for the EMBO Conference Pombe 2013, 7th International Fission Yeast Meeting, London, UK, 2013 - Member of the Program Committee for the 11th and 12th Greta Pifat Mrzljak International School of Biophysics, Primošten, Croatia, 2012 and 2014 Ad hoc reviewer: - Journals: Nature, Nature Cell Biology, eLife, Proceedings of the National Academy of Sciences, Current Biology, Molecular Biology of the Cell, EMBO Journal, Optics Express, Physical Biology, Journal of Microscopy, Biochemical and Biophysical Research Communications - Funding agencies: German Research Foundation (DFG), Human Frontier Science Program, Swiss National Science Foundation, Academy of Sciences of the Czech Republic Editorial activities: - Member of the Advisory Editorial Board of Life Science Alliance - Member of the Scientific Advisory Board and the Editorial Board of Science Matters Public outreach and media coverage: - Hosted the President of the European Research Council (ERC), Prof Jean-Pierre Bourguignon, in the lab in March 2018 - Hosted the President of the Republic of Croatia, Kolinda Grabar-Kitarović, in the lab in March 2017 - Speech to the European Parliament in Brussels, June 2015 - Guest in Croatian TV shows Treći element, Društvena mreža, and Među nama; interviews for TV shows Labirint, Znanstveni krugovi, and Dobro jutro, Hrvatska; numerous short interviews for TV news on Croatian Radiotelevision 1, Nova TV and RTL - Interviews on the radio: BBC World Service, Croatian Radio International Channel "The Voice of Croatia", Croatian Radio 3, Radio Sljeme, Radio Yammat - Interviews in newspapers and magazines: NASA's Astrobiology Journal (USA); Bild (Germany); Science et Vie (France); Croatia Airlines Inflight Magazine, Jutarnji list, Večernji list, Gloria, Novi List, Slobodna Dalmacija, 24 sata, Tehnopolis, Supermila (Croatia) - 5 popular science lectures - Chosen as one of the "Stars of 2015" by Gloria - Chosen as one of "The Most Stylish Scientists" by Future-ish, 2013-2015
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Iva Tolić Prof. dr. sc. Radionice 2 Institut Ruđer Bošković Bijenička cesta 54 Zagreb Hrvatska - Obrazovanje - Područja znanosti - Specifični znanstveni interesi - Projekti - Nagrade i priznanja - Nastava - Istaknute publikacije - Članstva u profesionalnim udrugama / društvima - Ostalo Obrazovanje - Doktor znanosti, polje biologija, Sveučilište u Zagrebu, 2002. godine - Izrada doktorske disertacije kod profesora Ninga Wanga na Harvardskoj školi javnog zdravstva (1999 – 2001), Boston, MA, SAD - Znanstvena novakinja u grupi akademika Nenada Trinajstića na Zavodu za fizičku kemiju Instituta Ruđer Bošković (1997 – 1999) - Diplomirani inženjer biologije, Sveučilište u Zagrebu, 1996. godine - Matura, Matematičko-informatički obrazovni centar (MIOC) u Zagrebu, 1992. godine Projekti - 2015 – 2020: ERC Consolidator Grant A new class of microtubules in the spindle exerting forces on kinetochores. Ukupna vrijednost projekta: 2.150.000.- EUR. - 2015 – 2019: Oscilatory dynamics of the cytoskeleton. Projekt koji financira Hrvatska zaklada za znanost (HRZZ). Ukupna vrijednost projekta: 1.000.000.- HRK. - 2015 – 2016: Cytoskeletal dynamics and spindle assembly during cell division. Projekt koji financira Europski socijalni fond (ESF); mentor zajedno s Igorom Weberom (PI). Ukupno odobreno: 1.560.000.- HRK. - 2013 – 2016: Kinetochore oscillations in mitotic metaphase. Projekt koji financira Njemačka Zaklada za Znanost (DFG); u suradnji s prof. Nenadom Pavinom sa Sveučilišta u Zagrebu. Ukupna vrijednost projekta: 256.000.- EUR. - 2013 – 2015: The role of microtubule pivoting in formation of complex structures such as microtubule bundles and mitotic spindles. Projekt koji je financirao Fond "Jedinstvo uz pomoć znanja"; u suradnji s prof. Nenadom Pavinom (PI) sa Sveučilišta u Zagrebu. Ukupna vrijednost projekta: 1.110.000.- HRK. - 2012 – 2015: Homologous chromosome pairing in Schizosaccharomyces pombe. Projekt koji je financirala Njemačka Zaklada za Znanost pod programom SPP 1384: Mechanisms of Genome Haploidization. Ukupna vrijednost projekta: 304.400.- EUR. - 2010 – 2014: Molecular architecture and mechanical properties of the kinetochore: a biophysical approach. Projekt koji je financirao Human Frontier Science Program (HFSP); u suradnji s prof. Jurajom Greganom (Sveučilište u Beču) i Danielom Cimini (Virginia Tech). Ukupna vrijednost projekta: 1.000.000.- $. - 2009 – 2012: Homologous chromosome pairing in Schizosaccharomyces pombe. Projekt koji je financirala Njemačka Zaklada za Znanost pod programom SPP 1384: Mechanisms of Genome Haploidization. Ukupna vrijednost projekta: 252.600.- EUR. - 2009 – 2012: In vitro reconstitution of motor-driven nuclear oscillations. Projekt koji je financirala Njemačka Zaklada za Znanost; u suradnji s prof. Stefanom Diezom (MPI-CBG u Dresdenu). Ukupna vrijednost projekta: 304.800.- EUR. - 2008 – 2012: Meiotic nuclear oscillations in Schizosaccharomyces pombe. Projekt koji je financirala Njemačka Zaklada za Znanost. Ukupna vrijednost projekta: 246.600.- EUR. - 2007 – 2011: Mechanisms of mitotic spindle alignment by cytoplasmic microtubules and cell shape in Schizosaccharomyces pombe. Projekt koji je financirala Njemačka Zaklada za Znanost. Ukupna vrijednost projekta: 242.600.- EUR. Nagrade i priznanja - Državna nagrada za znanost, 2016 - Nagrada "Utjecajne hrvatske žene", 2016 - Posebno priznanje Instituta Ruđer Bošković za izniman doprinos u jačanju znanstvene izvrsnosti i ugleda Instituta u međunarodnoj znanstvenoj zajednici te rezultate ostvarene u sklopu prestižnog projekta Europskog istraživačkog vijeća (ERC), 2015 - Medalja i nagrada Udruženja europskih biofizičkih društava za izvanredne znanstvene doprinose u području biofizike, 2015 - 5000.-a dobitnica ERC projekta - Nagrada MRAK za kreativnost u znanosti, za suradnju s Institutom Ruđer Bošković, 2015 - Red Danice hrvatske s likom Ruđera Boškovića za osobite zasluge za znanost i njezino promicanje u Republici Hrvatskoj i svijetu, 2014 - Nagrada "Europska nagrada za znanost o životu" u kategoriji istraživač godin, 2014 - Prema izboru časopisa Cell izabrana kao jedna od 40 znanstvenika mlađih od 40 godina iz cijelog svijeta i iz različitih područja biologije , "40 under 40", 2014 - 2001 – 2002: NATO stipendija - 1994: Rektorova nagrada, Sveučilište u Zagrebu - 1994 – 1995: Stipendija Grada Zagreba - 1988, 1989: Prvo mjesto na Republičkom natjecanju iz matematike Nastava - Poslijediplomski studij biofizike na Sveučilištu u Splitu - Kolegij "Molekularna citogenetika" na PMF-u u Zagrebu, gost predavač - Kolegij "Biofizika stanice" na PMF-u u Zagrebu, gost predavač - Praktikum iz biofizike stanice na PMF-u u Zagrebu Istaknute publikacije - Kajtez, J., A. Solomatina, M. Novak, B. Polak, K. Vukušić, J. Rüdiger, G. Cojoc, A. Milas, I. Šumanovac Šestak, P. Risteski, F. Tavano, A.H. Klemm, E. Roscioli, J. Welburn, D. Cimini, M. Glunčić, N. Pavin, and I.M. Tolić. (2016) Overlap microtubules link sister k-fibers and balance the forces on bioriented kinetochores. Nat. Preuzmi PDF - Coelho, M., S.J. Lade, S. Alberti, T. Gross, and I.M. Tolić. (2014) Fusion of protein aggregates facilitates asymmetric damage segregation. Preuzmi PDF - Ananthanarayanan, V., M. Schattat, S.K. Vogel, A. Krull, N. Pavin, and I.M. Tolić-Nørrelykke. (2013) Dynein motion switches from diffusive to directed upon cortical anchoring. Preuzmi PDF - Kalinina, I., A. Nandi, P. Delivani, M.R. Chacón, A.H. Klemm, D. Ramunno-Johnson, A. Krull, B. Lindner, N. Pavin, and I.M. Tolić-Nørrelykke. (2013) Pivoting of microtubules around the spindle pole accelerates kinetochore capture. Nat. Preuzmi PDF - Stiess, M., N. Maghelli, L.C. Kapitein, S. Gomis-Rüth, M. Wilsch-Bräuninger, C.C. Hoogenraad, I.M. Tolić-Nørrelykke, and F. Bradke. (2010) Axon extension occurs independently of centrosomal microtubule nucleation. Recommended by Faculty of 1000. Preuzmi PDF - Ukupni broj radova: 65 - Zbirni faktor odjeka (IF) časopisa: 400 - Ukupni broj citata: 2400 (Web of Science), 2600 (Scopus), 3800 (Google Scholar) - h-index: 21 (Web of Science), 22 (Scopus), 28 (Google Scholar) Članstva u profesionalnim udrugama / društvima - Hrvatsko biofizičko društvo - Hrvatsko društvo za biokemiju i molekularnu biologiju - Hrvatsko mikroskopijsko društvo Ostalo Istraživačko i znanstveno iskustvo: - Znanstvena savjetnica na Institutu Ruđer Bošković (od 2014) - Voditeljica istraživačke grupe W2 (ekvivalentno mjestu više znanstvene suradnice) na Institutu Max Planck za molekularnu staničnu biologiju i genetiku u Dresdenu (2010 – 2014) - Voditeljica istraživačke grupe W1 (ekvivalentno mjestu znanstvene suradnice) na Institutu Max Planck za molekularnu staničnu biologiju i genetiku u Dresdenu (2004 – 2009) - Poslijedoktorsko usavršavanje u grupi profesora Francesca Pavonea u Europskom laboratoriju za nelinearnu spektroskopiju u Firenci (2003 – 2004) - Poslijedoktorsko usavršavanje u grupi profesorice Kirstine Berg-Sørensen na Institutu Niels Bohr u Kopenhagenu (2001 – 2002) Nedavna otkrića: - Diobeno vreteno je minijaturni stroj sastavljen od mikrotubula koji dijele kromosome u dvije stanice kćeri. Veza između mikrotubula i kromosoma su kinetohore, proteinski kompleksi na kromosomu. Ključno je pitanje kako nastaju sile koje djeluju na kinetohore. Smatra se da sile napetosti između sestrinskih kinetohora stvaraju snopovi mikrotubula koji završavaju na kinetohori, nazvani k-vlaknima. U ovom smo radu otkrili novu skupinu mikrotubula koji stvaraju sile na kinetohore, a koje smo nazvali premosnim mikrotubulima jer premošćuju sestrinska k-vlakna. Vezu između premosnih mikrotubula i sestrinskih k-vlakana dokazali smo eksperimentima u kojima smo laserom prerezali k-vlakno, nakon čega smo opazili da se premosno vlakno kreće zajedno s k-vlaknima. Kombinirajući novi teorijski model s eksperimentima, pokazali smo da se napetost između kinetohora smanjuje ako je k-vlakno rezano bliže kinetohori, što dovodi do zaključka da premosno vlakno uravnotežuje napetost između kinetohora povezujući sestrinska k-vlakna. Ovaj rad preispituje trenutnu paradigmu prema kojoj sile napetosti između kinetohora ovise samo o molekularnim događajima na krajevima k-vlakana, te ukazuje na to da će se postojeći modeli diobenog vretena morati proširiti tako da uključe sile koje stvara premosno vlakno (Kajtez et al., Nat Commun 2016, IF=11.5). - Dok starenje ostaje neizbježna činjenica života, pokazali smo da kvasac S. pombe ne podliježe replikativnom starenju. Poznato je da se čak i mikrobi koji se naizgled simetrično dijele, ne dijele u dvije točno identične stanice kćeri, nego jedna dobiva stariji, često neispravni, stanični materijal i stoga stari, dok je druga opremljena novim potpuno funkcionalnim materijalom, te je tako pomlađena. Naš rad pokazuje da je, za razliku od drugih vrsta kvasaca, S. pombe imun na starenje kada se reproducira pod povoljnim uvjetima rasta, jer tada obje stanice kćeri nasljeđuju jednak udio starog staničnog materijala. Kad se pak S. pombe podvrgne negativnim utjecajima poput oksidansa ili visoke temperature, počne se dijeliti na mlađe i starije stanice kćeri baš kao i ostale vrste stanica. Dok starije stanice na kraju umru, mlađe se reproduciraju čak i u teškim uvjetima. Naši su rezultati istaknuli S. pombe kao zanimljiv organizam koji bi mogao poslužiti kao model za ljudske stanice koje ne stare, poput zametnih stanica, matičnih stanica i stanica raka. Naš je rad doveo u pitanje dogmu da svi organizmi, uključujući mikroorganizme, stare (Coelho et al., Curr Biol 2013, IF=9.5; Coelho et al., PLOS Biol 2014, IF=12.7). - Stanice imaju fascinantnu osobinu da uredno organiziraju svoju unutrašnjost uz pomoć motornih proteina. Takav jedan protein poznat pod imenom citoplazmatski dinein pokreće ključne procese u stanicama, kao što su transport tvari u neuronima i formiranje diobenog vretena. Ipak, malo se zna o ponašanju dineina u živoj stanici. Moja je grupa razvila metodu promatranja dineina na razini pojedinačne molekule u živoj stanici. Uz pomoć fluorescencijske mikroskopije potpune unutarnje refleksije promatrali smo dineine kako se kreću kroz stanicu, vežu za mikrotubul i hodaju po njemu. Otkrili smo da se dinein veže u dva koraka, prvo na mikrotubul, a potom i na proteine sidra u staničnoj kori. Pokazali smo da dineini pronalaze sidra tako što difundiraju uzduž mikrotubula, a prilikom vezanja na sidro počinju se kretati usmjereno. Ova nova i tehnički zahtjevna zapažanja na razini pojedinačnih molekula objašnjavaju kako dinein pronalazi mjesta s kojih djeluje silom na jezgru, te mehanizam aktivacije dineina (Ananthanarayanan et al., Cell 2013, IF=32.0). - Da bi u stanici majci došlo do podjele genetskog materijala u dvije stanice kćeri, kromosomi se vezuju za mikrotubule, koji ih potom razdvajaju. Veza između mikrotubula i kromosoma su kinetohore, proteinski kompleksi na kromosomu. Ključno je pitanje kako mikrotubuli pronalaze kinetohore. Prema ideji "traži i uhvati", koja prevladava u području, brojni mikrotubuli rastu u svim smjerovima i slučajno hvataju kinetohore. Mi smo otkrili novi mehanizam hvatanja kinetohore tijekom mitoze, koji se temelji na vrtnji mikrotubula oko centrosoma (Kalinina et al., Nat Cell Biol 2013, IF=20.8). Pokazali smo da mikrotubuli svojim rotacijskim kretanjem prebrišu prostor te na taj način pretražuju stanicu u potrazi za kinetohorama. Ovaj novi mehanizam hvatanja kinetohore preispituje paradigmu o uhvatu kromosoma uvedenu 80-tih godina prošloga stoljeća. Vođenje doktorata, magistarskih i diplomskih radova: - 6 studenata doktoriralo, 4 magistriralo i 6 diplomiralo . - Od 6 doktoranada, 4 su objavili znanstvene radove kao prvi autori u vodećim znanstvenim časopisima (Cell, Nature Cell Biology, Current Biology, PLoS Biology). Trenutno vodi 4 doktoranda. - Ukupno je vodila 13 postdoktoranada. Pozvana predavanja: Više od 60 pozvanih predavanja na konferencijama i znanstvenim radionicama uključujući: EMBO Conference on Meiosis, Oxford, Velika Britanija, 2015 12th Multinational Congress on Microscopy, Eger, Mađarska, 2015 (plenarno predavanje) 10th European Biophysics Congress EBSA 2015, Dresden, Njemačka (plenarno predavanje) The American Society for Cell Biology Annual Meeting, Philadelphia, PA, SAD, 2014 Biophysical Society 56th Annual Meeting, San Diego, CA, SAD, 2012 Gordon Research Conference: Plant and Microbial Cytoskeleton, Andover, NH, SAD, 2012 Više od 40 pozvanih predavanja na kolokvijima i seminarima uključujući: ETH Zürich, Švicarska, 2015 Graduate Student Symposium of the MRC Laboratory of Molecular Biology, Cambridge, Velika Britanija, 2013 Engineering and Physical Biology Symposium, Harvard, Cambridge, MA, SAD, 2012 Institut Curie, Pariz, Francuska, 2011 California Institute of Technology, Pasadena, CA, SAD, 2010 Organizacija konferencija: - Organizatorica konferencije EMBO Conference on Meiosis, Hvar, Hrvatska, 2017 - Članica međunarodnog savjetodavnog odbora konferencije From Solid State to Biophysics VIII, Cavtat, Hrvatska, 2016 - Članica organizacijskog odbora EMBO Conference on Meiosis, Oxford, Velika Britanija, 2015 - Predsjedateljica simpozija Molecular Motors, 10th European Biophysics Congress, EBSA 2015, Dresden, Njemačka - Predsjedateljica plenarnog simpozija Cell Morphology u sklopu EMBO Conference Pombe 2013, 7th International Fission Yeast Meeting, London, Velika Britanija - Članica programskog odbora 11. i 12. Međunarodne škole biofizike Greta Pifat-Mrzljak, Primošten, Hrvatska, 2012 i 2014 Recenzentske aktivnosti: - Više od 40 recenzija u stranim znanstvenim časopisima: Nature, Nature Cell Biology, Proceedings of the National Academy of Sciences, Current Biology, eLife, Molecular Biology of the Cell, EMBO Journal, Optics Express, Physical Biology, Journal of Microscopy, Biochemical and Biophysical Research Communications. - Više od 5 recenzija stranih znanstvenih projekata: German Research Foundation (DFG), Human Frontier Science Program (HFSP), Swiss National Science Foundation, Academy of Sciences of the Czech Republic. Uređivanje znanstvenih časopisa: - Članica Uredništva i Znanstvenog savjetodavnog odbora u znanstvenom časopisu Science Matters. Popularizacija znanosti: - Govor u Europskom parlamentu, 2015 - Televizija: gost u studiju u emisijama "Treći element", "Društvena mreža", "Među nama"; intervjui u emisijama "Labirint", "Znanstveni krugovi" i "Dobro jutro Hrvatska" na HTV, "Bolja Hrvatska" na Novoj TV, "RTL Danas" na RTL-u - Radio: intervjui na BBC World Service, Hrvatskom radiju 3, Radio Sljemenu, u Međunarodnom programu Hrvatskoga radija "Glas Hrvatske" i na Radio Yammatu - Tisak: intervjui u novinama i časopisima kao što su Jutarnji list, Večernji list, Gloria, Putni časopis Croatia Airlinesa, Novi list, Slobodna Dalmacija, 24 sata, Glas Koncila, Tehnopolis, Supermila, Bild, NASA's Astrobiology Journal, Science et Vie - 5 popularno-znanstvenih predavanja - Uvrštena među Zvijezde 2015. godine po izboru časopisa Gloria - Uvrštena na listu znanstvenica s najviše stila u svijetu po izboru organizacije Future-ish, 2013-2015