http___www.irb.hr_Istrazivanja_Zavodi-i-centri_Zavod-za-molekularnu-medicinu_2.xml
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Division of Molecular Medicine The mission of the Division of Molecular Medicine is to expand and enhance our knowledge of the nature of diseases and to develop and improve new strategies for the diagnosis, treatment and prevention of disease. Acting Head Neven Soić Administration The mission of DMM is closely correlated with the major areas of human medicine, covering a broad spectrum reflected in the name of its laboratories. The majority of research topics have been oriented to the molecular aspects of cancer, neuroscience (neuropsychiatry, neurodegeneration, neuropharmacology), stress-related diseases and their models. Particular attention and research has been devoted to mechanisms of disease etiology through disturbed methylation of macromolecules (DNA/proteins), molecular mechanisms of disease etiopathogenesis and experimental therapy. The DMM, with its Human Tumor Bank and numerous clinical samples collected through collaborations with clinicians (e.g. in neuropsychyatry and neurodegeneration), represents an important biomedical core, dedicated to establishing and refining new strategies and methods for understanding the molecular mechanisms of disease, for improving diagnostic procedures and therapies and, of primary importance, for disease prevention. Main interest of the Laboratory is examination of the selected membrane-bound (aminopeptidase-N, neutral endopeptidase, dipeptidyl-peptidase-4) and intracellular peptidase (dipeptidyl-peptidase-9) in regulation of immune and inflammatory responses, as well as in growth and spreading potential of tumor cells.more » Research activity in LET has been dealing with tumor cells and their response to therapy.more » Laboratory of Molecular Neuropsychiatry investigates the role of endocrine and neurotransmitter systems and their corresponding genes in the etiology and treatment of neuropsychiatric and stress related disorders.more » The lab focuses on molecular analysis of infectious diseases, primarily human papillomaviruses (HPV) and cervical cancermore » Major research directions of the Laboratory for Hereditary Cancer are: - Basic scientific research of signal transcduction with special emphasis on implementation of new research technologies; - Applied scientific research of cancer and hereditary diseases focused on procedures which can be used in diagnostics of hereditary diseases and cancer therapy; - Development of bioinformatics and in silico modeling based on the results of main research directions Major activities - Studies on physiology and pathology of oxidative stress and in particular lipid peroxidation - Molecular aspects of growth regulating activities of lipid peroxidation product 4-Hydroxynonenal (HNE) - Experimental diagnostics and therapies of malignant diseases associated with oxidative stress - Experimental therapies of cancer - Cell and tissue culture models Research conducted in the LPMed involves the study of molecular and genetic mechanisms underlying colorectal cancer development and progression as well as pharmacogenetic studies associated with the effectivness and toxicity of cancer therapies.more » Our facility breeds inbreed strains of mice (BALB/cBkl, CBA/H, C3Hf/Bu, C57BL/Go, C57BL/6-Ly5, NOD, Hsd:ICR (CD-1)) for the research projects conducted mainly in Rudjer Boskovic Institute. In our animal unit two colonies of rats are developed from the Wistar stock, i.e. “Hyperserotoninemic rat” and “Wistar-Zagreb 5HT rat”. Both models are used in neuroscience research as rodent models for selected neuropsychiatric disorders as well as in related neuropsychopharmacological research.more » Luka Andrišić Tihomir Balog PhD Robert Belužić Maja Pokas Suzana Borović Šunjić PhD Vesna Bošnjak Tina Catela Ivković Mario Cindrić Margareta Cvetkovski Tamara Čačev PhD, MBA Mirsada Čaušević PhD, Marie Curie Fellow Stjepko Čermak Ana Čipak Gašparović PhD Barbara Čupić PhD Katja Ester dr.sc. Koraljka Gall-Trošelj MD, Ph.D. Goran Goleš Jasminka Golubić Talić Magdalena Grce PhD Mirko Hadžija Maja Herak Bosnar PhD Nevenka Hiršl Anđela Horvat Anita Horvatić Chemistry Morana Jaganjac PhD Maja Jazvinšćak Jembrek PhD Sanja Kapitanović MD, PhD Silva Katušić Hećimović Jelena Knežević dr.sc. Marko Košiček PhD Marijeta Kralj PhD Borka Kušić PhD senior research associate Ivana Levak Sonja Levanat Željka Mačak Šafranko PhD Martina Malnar PhD Marko Marjanović PhD Marina Marš Tanja Matijević Glavan PhD Nina Milutin Gašperov PhD Vesna Musani PhD Gordana Nedić Erjavec PhD Matea Nikolac Perković PhD Renata Novak Kujundžić Višnja Novalić Petar Ozretić PhD Zlatko Pandžić Jasminka Pavelić PhD Martina Pehar Nela Pivac DVM, PhD Marijana Popović Hadžija PhD Ivan Sabol PhD Maja Sabol PhD Neda Slade Sandra Sobočanec PhD Višnja Stepanić Ranko Stojković PhD., D.M.V. Dubravka Švob Štrac Diana Trnski Josipa Vlainić Ankica Vratarić Oliver Vugrek PhD Tea Vuković Emilija Zapletal PhD Arijana Zorić Neven Žarković MD,PhD A multidisciplinary approach to discover selective drugs targeting cancer stem cells: The role of potassium transport - MultiCaST The main goal of the proposed research is to understand cancer stem cell (CSC) biology and to develop novel CSC-directed compounds. We will strive to elucidate the molecular basis of potassium ion transport involvement in CSC-phenotype acquisition, modulation and targeting and identify key mechanisms of these processes as novel biomarkers for improved diagnostic options and innovative mechanism-based therapeutic approaches.more » Aberrant DNA methylation in HPV associated lesions Alzheimer's disease - the role of cholesterol on processing and localization of APP-family members This project aims to analyze whether the molecular mechanisms of the cholesterol effect on APP and amyloid-B peptide (AB) may involve APP-family members, APLP1 and APLP2. We will elucidate whether cholesterol affects processing of APLP1 and APLP2, like APP, and whether the cholesterol effect on APP is mediated by APLP1/2-regulated trafficking of APP.more » Automatization of proteomics-driven biotypization Bacteria biotyping using modern proteomics approach BrainProtect - Presenilin 2 - a protector against Alzheimer's disease Marie Curie Actions - Intra-European Fellowship for Career Development (IEF) FP7-PEOPLE-2013-IEFmore » COST Action CM1001 - Chemistry of non-enzymatic protein modification - modulation of protein structure and function COST-Structure-based drug design for diagnosis and treatment of neurological diseases: dissecting and modulating complex function in the monoaminerg... Cytochrome P450 monooxigenases system and tumor appearance in aging and oxidative stress Cytochrome P450 monooxygenases influence proliferation, differentiation, apoptosis or necrosis, cellular homeostasis as well as neuro-humoral functions by means of participating in the metabolism of a wide spectrum of endogenous and exogenous compounds, including therapeutic agents and toxicants. The expression of CYP gene products are of particular interest because of the role these enzymes play in the activation of exogenous procarcinogenes. Upregulation of CYP isoforms leads to ROS production, and possibly to modulation of the oxidant/antioxidant status which might be related to sex and age related tumor appearance. Thus, examining of P450 gene expression and translation to active enzymes could provide valuable informations toward understanding the mechanism of gender-associated sensitivity of various tissues (liver, lung and brain) to oxidative stress and possible association with tumor appearance in senescence.more » Detection and tracking of biological markers for early therapeutic intervention in sporadic Alzheimer's disease Detection and tracking of biological markers for early therapeutic intervention in sporadic Alzheimer's disease Development of novel approaches to proteomics analyses Effect of novel Cu-doped 45S5 bioactive glass and lipid peroxidation products on bone regeneration Effects of oxidatively modified collagen on breast cancer stem cell growth and differentiation Enhancement of the Innovation Potential in SEE through new Molecular Solutions in Research and Development - InnoMol The largest infrastructure project with the highest budget in natural sciences ever to be conducted in Croatia will foster a research pipeline at the RBI and facilitate innovation and technology for the investigation of relevant diseases such as cancer.more » Epigenetic and Immunomodulatory Changes in Malignant Head and Neck Tumours Gene therapy of tumors by modulating the molecules of the immune system Genetic factors as markers of suicide Implementation of new methods for identification of protein biomarkers; the first step in understanding, diagnostics and treatment of disease Improvement of adhesive properties of biomedical materials by plasma treatment Lipids, Free Radicals and their Messengers in Integrative Oncology Lysosomal dysfunction as a common mechanism of neurodegenerative diseases Using a lysosomal disorder NPC as a model, the goal of this project is to investigate a role of lysosomal impairment on accumulation of amyloid-beta peptide (Abeta) and alpha-synuclein (a-syn), the two characteristic features in the pathogenesis of AD and PD.more » Molecular basis and treatment of psychiatric and stress related disorders Stress related (acute stress reaction, posttraumatic stress disorder) and eating disorders, addictions (alcoholism), aggressive/suicidal behavior, and attention deficit hyperactivity disorder are frequent multifactorial and polygenic psychiatric disorders that induce a great suffering in patients and their families, and carry financial burden to the whole society. Molecular basis of these disorders involves the changes in neurotransmitter (primarily serotonin - 5-HT, noradrenalin, dopamine) and neuroendocrine systems, and functional gene polymorphisms controlling the activity of the corresponding proteins.more » Molecular genetics and pharmacogenetics of gastrointestinal tumors The main goal of this study is to investigate hereditary and sporadic genetic changes in benign and malignant gastrointestinal tumors in order to elucidate the mechanisms of their development and progression. Another goal of this study is to start with prospective pharmacogenetic study in order to examine the correlation between known polymorpisms and gastrointestinal cancer therapy.more » Molecular mechanism(s) of cholesterol-effect on APP and BACE1 metabolism - the two key proteins of Alzheimer's disease The goal of this project was to further elucidate the mechanism/s of cholesterol-effect on the metabolism of APP and BACE1 – the two key proteins in the pathogenesis of Alzheimer's disease. Using biochemical, molecular biological and cell biological approaches we have tested the hypotheses that alterations in cholesterol metabolism (due to NPC1 dysfunction) contribute to accumulation of Abeta and the pathogenesis of AD by modulating endocytic trafficking of BACE1 and/or by modulating lysosomal/autophagic function.more » Pharmacogenomics and proteomics of serotonergic and catcholaminergic system Serotonergic and catecholaminergic (dopaminergic, noradrenergic) neurotransmitter systems regulate physiological functions and are also connected to the etiology of complex and polygenic neuropsychiatric disorders (Alzheimer’s disease /AD/, frontotemporal dementia, schizophrenia and depression). Etiology and psychobiological mechanisms of neuropsychiatric disorders is not well understood, but could be the result of interaction of genetic factors, specific proteins and environment.more » Pharmacogenomics, proteomics and psychophisiology of neuropsychiatric disorders ProNetMel - New Protein Networks for Novel Therapeutic Avenues in Human Melanoma The main focus of this project is to reveal interactions of p53 with protein partners in melanoma that are capable of modifying its function. We are particularly interested in possible interactions of p53 with family members, namely p53 and p73 isoforms, with nm23, especially nm23-H1 and nm23-H2, and Gli family of proteins.more » Protemic analysis of tissue samples in patients with prostatic carcinoma RapidCell - Rapid Identification of Cells in a Clinical Environment Stress, GABA-A receptors and mechanisms of action of neuropsychoactive drugs The purpose of the present research is to improve our understanding of the mechanisms of action of neuropshycoactive drugs, especially during their chronic administration and modification by stress. Particular emphasis is placed on the research of benzodiazepines and antidepressants, used to treat anxiety, insomnia and depression, which are acting via GABAergic and serotonergic systems in the brain. Therefore, we investigate the changes in the expression and function of GABA-A receptor in vitro after prolonged exposure to benzodiazepines, known to induce the development of tolerance and dependence. Moreover, by using experimental animals, we have been also investigating the interactions between the effects of stress and benzodiazepines and antidepressants, primarily on the susceptibility to convulsions, as well as the role of the serotonergic system in controlling brain excitability.more » Synthesis of disulfoxy and trisulfoxy derivatization analogs for unambiguous peptide and protein identification by mass spectrometry The mechanism of cholesterol action in the pathogenesis of Alzheimer’s disease Recent studies reveal that cholesterol may play a role in the pathogenesis of Alzheimer’s disease (AD). The goal of this project is to elucidate the mechanism(s) of cholesterol-effect on APP metabolism, formation of amyloid-B peptide (AB) and the genesis of Alzheimer’s disease. We speculate that cholesterol regulation of membrane and protein trafficking along the endocytic pathway leads to amyloidogenic processing of APP and AB formation.more » The molecular links between cholesterol homeostasis, membrane trafficking and Alzheimer's disease Aberrant proteolytic cleavage of the amyloid precursor protein (APP) leading to increased formation/accumulation of B-amyloid peptide (AB) is considered a central event in the pahogenesis of Alzheimer's disease (AD). Although much has been known about the AB generation and its clearance, we still do not understand in great detail how AB metabolism is altered in the most common late-onset form of AD (LOAD) and what are the molecular trigger(s) that initiate these events. In this project we will test the hypothesis that cholesterol metabolism and membrane trafficking are tightly linked and that their dysregulation leads to Alzheimer's disease.more » The role of different cell death responses to DNA-damage treatment The role of membrane peptidases on tumor and normal cells The role of nm23 genes in oral squamous cell carcinoma The role of p53/p73 protein network in soft tissue sarcoma The role of Sirtuin1 in non-small cell lung cancer, NSCLC DNA Sequencing and Fragmental Analysis DNA Sequencing and Fragmental Analysismore » Genetic Testing for Hereditary Breast and Ovarian Cancer (BRCA1 and BRCA2 Genes) Genetic Testing for Hereditary Breast and Ovarian Cancer (Analysis of BRCA1 and BRCA2 Genes)more » Screening for mutations in the ret proto-oncogene This genetic analysis is indicated in all newly diagnosed medullary thyroid patients in order to exclude or confirm the existance of an inherited mutation. These analyses are based on the contract between Clinical Hospital Center "Sister of Charity" and Rudjer Boskovic Institute.more »
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Zavod za molekularnu medicinu Misija Zavoda za molekularnu medicinu je proširivanje znanja i razumijevanja prirode bolesti čovjeka te poboljšanje i razvijanje novih pristupa dijagnozi, liječenju i prevenciji bolesti. V.d. predstojnik dr. sc. Neven Soić Tajništvo Misija Zavoda za molekularnu medicinu je proširiti i ojačati naše poznavanje prirode bolesti, razviti i unaprijediti postojeće te razviti nove strategije za dijagnozu, liječenje i prevenciju bolesti. Ovi ciljevi se ostvaruju kroz istraživanja vezana uz procjene utjecaja genetičkih čimbenika u prevenciji bolesti, smanjenje čimbenika rizika te kroz razvoj i vrednovanje novih lijekova, istraživanje biokemijskih i staničnih učinaka lijekova na stanice i organizme, unapređivanje znanstvene metodologije, i obrazovanja znanstvenika i studenata. Zavod trenutno razvija nekoliko strateških projekata koji uključuju razvoj molekulske ciljane terapije protiv raka i virusnih bolesti, razvoj novih dijagnostičkih alata i istraživanja s ciljem dešifriranja molekularnih osnova bolesti, kao i razvoj alata temeljenih na "-omics" pristupu. Zavod se profilira kao centar izvrsnosti za istraživanja temeljenih na molekularnim pristupima u proučavanju bolesti. Članovi zavoda značajno doprinose visokom obrazovanju u Republici Hrvatskoj kroz pružanje brojnih tečajeva i održavanje nastave na dodiplomskoj i poslijediplomskoj razini u Zagrebu, Splitu, Osijeku, Rijeci, Dubrovniku i Zadru. Grupa za Translacijsku Medicinu koristi bolest nedostatka SAHH kao modelni sistem za proučavanje dinamičnih procesa u ljudskom epigenomu, t.j. istraživanje metilacija DNA i proteina.više » Laboratorij za eksperimentalnu hematologiju, imunologiju i onkologiju Laboratorij se bavi ispitivanjem uloge odabranih peptidaza (aminopeptidaza-N, neutralna endopeptidaza, dipeptidil peptidaza-4, dipeptidil peptidaza-9) u regulaciji imunih i upalnih reakcija, kao i u nastanku te progresiji tumora.više » Laboratorij se bavi različitim aspektima terapije tumora.više » Laboratorij se bavi molekularnim analizama infektivnih bolesti, posebice papiloma virusom čovijeka i rakom vrata materniceviše » Glavna istraživačka usmjerenja Laboratorija za nasljedni rak su: Temeljna znanstvena istraživanja vezana su za prijenos signala uz poseban naglasak na uvođenje novih istraživačkih tehnologija; Primijenjena znanstvena istraživanja vezana uz rak i nasljedne bolesti usmjerena su na razvoj postupaka koji mogu poslužiti u dijagnostici nasljednih bolesti, te u terapiji raka; Razvoj bioinformatike i in silico modela na temelju rezultata glavnih istraživačkih usmjerenja.više » Glavna područja istraživanja su izučavanje fiziologije i patologije oksidacijskog stresa, molekularne značajke regulacije rasta putem 4-hidroksinonenala (HNE), eksperimentalna dijagnostika i terapija zloćudnih bolesti temeljenih na oksidacijskom stresu, bioterapija raka i uzgoj kultura tkiva kosti.više » Znanstvena djelatnost LPMed-a je usmjerena na istraživanja molekularno-genetičke osnove nastanka i napredovanja sporadičnih tumora debelog crijeva te farmakogenetske studije vezane uz učinkovitost ili toksičnost tumorske terapije.više » Laboratorij proučava spolne razlike u osjetljivosti na oksidacijski stres na mišjem modelu.više » Pogon laboratorijskih životinja (PLŽ) djeluje u sklopu Zavoda za molekularnu medicinu Instituta «Ruđer Bošković». U Pogonu laboratorijskih životinja se uzgajaju jezgre pet srođenih mišjih sojeva BALB/c, CBA/H C3Hf/Bu, C57BL/6-Ly5 i NOD, kao i jedan nesrođeni soj HSD:ICR (CD-1), te štakori (Wistar Zagreb 5HT - podsoj selekcioniran prema razini perifernog serotonina). Životinje se uzgajaju na način koji omogućava genetsku stabilnost sojeva, a životinje se nalaze pod stalnim veterinarskim nadzorom zdravstvenog stanja.više » Luka Andrišić dipl.ing Tihomir Balog Dr.sc Mirela Baus Lončar dr.sc. Robert Belužić dr.sc. Maja Boranić Suzana Borović Šunjić dr.sc. Vesna Bošnjak Maro Bujak dr.sc. Tina Catela Ivković dipl.ing. Mario Cindrić dr. sc. Margareta Cvetkovski Tamara Čačev dr.sc. Mirsada Čaušević dr.sc., Marie Curie Fellow Stjepko Čermak dr.med.vet. Ana Čipak Gašparović dr.sc. Barbara Čupić dr.sc. Martina Deželjin dr.sc Kristina Dominko mag.biol.mol. Katja Ester dr.sc. Koraljka Gall Trošelj dr. sc. Goran Goleš Jasminka Golubić Talić Ivana Grbeša dr. sc. Magdalena Grce dr.sc. Mirko Hadžija dr.sc. Maja Herak Bosnar dr.sc. Nevenka Hiršl Anđela Horvat dipl. ing. Anita Horvatić dr. sc. Morana Jaganjac dr. sc. Maja Jazvinšćak Jembrek dr.sc. Sanja Kapitanović dr.sc., dr.med. Silva Katušić Hećimović dr.sc. Tea Kečkeš Jelena Knežević dr.sc. Marina Korolija dr. sc. Marko Košiček dr.sc. Marijeta Kralj dr. sc. Borka Kušić dr. sc. Ivana Levak Sonja Levanat dr. sc. Željka Mačak Šafranko dr.sc. Martina Malnar dr. sc. Marko Maričević Marko Marjanović dr.sc. Marina Marš Irena Martin Kleiner dr.sc. Tanja Matijević Glavan dr. sc. Ana-Matea Mikecin dr.sc. Lidija Milković dr.sc. Nina Milutin Gašperov dr. sc. Vesna Musani dr.sc. Gordana Nedić Erjavec dr. sc. Matea Nikolac Perković dipl. ing. Renata Novak Kujundžić dr.sc. Višnja Novalić Petar Ozretić dr. sc. Zlatko Panđić Jasminka Pavelić dr. sc. Martina Pehar Nela Pivac dr sc Marijana Popović Hadžija dr.sc. Ivan Sabol Dr. sc. Maja Sabol dr.sc. Neda Slade dr. sc. Sandra Sobočanec dr.sc. Višnja Stepanić dr.sc. Tamara Stipčević dr.sc. Ranko Stojković dr.sc., dr.med.vet. Dubravka Švob Štrac dr.sc. Diana Trnski dipl.inž. Lidija Uzelac dipl. ing. Josipa Vlainić dr.sc. Ankica Vratarić Oliver Vugrek dr. sc. Tea Vuković Emilija Zapletal dr. sc. Arijana Zorić dr.sc. Neven Žarković A multidisciplinary approach to discover selective drugs targeting cancer stem cells: The role of potassium transport - MultiCaST The main goal of the proposed research is to understand cancer stem cell (CSC) biology and to develop novel CSC-directed compounds. We will strive to elucidate the molecular basis of potassium ion transport involvement in CSC-phenotype acquisition, modulation and targeting and identify key mechanisms of these processes as novel biomarkers for improved diagnostic options and innovative mechanism-based therapeutic approaches.više » Alzheimerova bolest - uloga kolesterola na procesiranje i lokalizaciju obitelji proteina APP Cilj ovog projekta je ispitati je li kolesterol modulira proteolitičku razgradnju i drugih članove obitelji proteina APP, proteine APLP1 i APLP2. Osim toga, ispitat ćemo je li učinak kolesterola na metabolizam proteina APP posredovan djelovanjem proteina APLP1/2 na proteolitičku razgradnju i/ili na unutarstanični transport proteina APP.više » Automatizacija postupka određivanja bioloških vrsta primjenom proteomike Biotipizacija bakterija suvremenim proteomskim pristupom COST Action CM1001 - Chemistry of non-enzymatic protein modification - modulation of protein structure and function COST- Structure-based drug design for diagnosis and treatment of neurological diseases: dissecting and modulating complex function in the monoaminer... Djelovanje plazme na poboljšanje adhezivnih svojstava biomedicinskih materijala Dobivanje struktura nalik Langerhansovim otočićima iz matičnih stanica miša Enhancement of the Innovation Potential in SEE through new Molecular Solutions in Research and Development - InnoMol The largest infrastructure project with the highest budget in natural sciences ever to be conducted in Croatia will foster a research pipeline at the RBI and facilitate innovation and technology for the investigation of relevant diseases such as cancer.više » Epigeničke i imunomodulatorne promjene u zloćudnim tumorima glave i vrata Farmakogenomika i proteomika serotoninskog i kateholaminskog sustava Serotoninski i kateholaminski (dopaminski i noradrenalinski) neurotransmitorski sustavi reguliraju fiziološke funkcije u organizmu, a povezuju se i s etiologijom složenih, poligenetskih neuropsihijatrijskih poremećaja kao što su Alzheimerova bolest (AD), frontotemporalna demencija, shizofrenija i depresija. Etiološka i psihobiološka podloga neuropsihijatrijskih poremećaja nije u potpunosti jasna, a mogla bi biti posljedica međusobnog djelovanja genetskih čimbenika, specifičnih proteina i utjecaja okoline.više » Farmakogenomika, proteomika i psihofiziologija neuropsihijatrijskih poremećaja Znanstveni programviše » Genetski markeri kao pokazatelji suicida Gensko liječenje tumora djelovanjem na molekule imunološkog sustava Implementacija novih metoda za identifikaciju proteinskih biomarkera; prvi korak u razumijevanju, diagnostici i liječenju bolesti Lipidi, slobodni radikali i njihovi glasnici u integrativnoj onkologiji Mehanizam djelovanja kolesterola u nastanku Alzheimerove bolesti Nedavna istraživanja ukazuju na ulogu kolesterola u nastanku Alzheimerove bolesti (AB). Cilj ovog projekta je utvrditi mehanizam djelovanja kolesterola na metabolizam proteina APP, stvaranje peptida AB i nastanak Alzheimerove bolesti. Pretpostavljamo da regulacijom transporta membrana i proteina u endosomalnom putu kolesterol regulira proteolitičku razgradnju proteina APP i stvaranje peptida AB.više » Molekularna genetika i farmakogenetika gastrointestinalnih tumora Cilj ovog istraživanja je ispitati nasljedne i sporadične genetske promjene u gastrointestinalnim tumorima radi boljeg razumijevanja mehanizama njihovog nastanka i napredovanja kao i uspostaviti prospektivnu farmakogenetičku studiju kojom bi se ispitala korelacija između poznatih polimorfnih biljega i terapije gastrointestinalnih karcinoma.više » Molekularna podloga i liječenje psihijatrijskih i stresom izazvanih poremećaja Stresom izazvani poremećaji (akutni stresni poremećaj, posttraumatski stresni poremećaj), ovisnosti (alkoholizam), agresivno/suicidalno ponašanje, poremećaji hranjenja i deficit pažnje i hiperaktivni poremećaj su česti, multifaktorijalni i poligenski psihijatrijski poremećaji. Oni izazivaju značajnu patnju bolesnika i njihovih obitelji i opterećuju troškovima cijelo društvo. Molekularna podloga tih poremećaja uključuje promjene neurotransmitorskih (prvenstveno serotonina - 5-HT, noradrenalina, dopamina) i neuroendokrinih sustava i funkcionalne polimorfizme gena koji kontroliraju aktivnost pripadajućih proteina. više » Molekularna povezanost između homeostaze kolesterola, membranskog transporta i Alzheimerove bolesti Molekularni mehanizam djelovanja kolesterola na metabolizam proteina APP i BACE1 - dva ključna proteina Alzheimerove bolesti Cilj ovog projekta je razjasniti mehanizam kojim kolesterol utječe na metabolizam proteina APP i BACE1 - dva ključna proteina u patogenezi Alzheimerove bolesti. Kombinacijom biokemijskih, molekularno bioloških i stanično bioloških pristupa, istražili smo hipotezu da povećana razina kolesterola uzrokovana nefunkcionalnošću proteina NPC1 doprinosi nakupljanju peptida Ab.više » Nedostatak Adenozilhomocistein hidrolaze: Molekularni mehanizmi novog oboljenja Presenilin 2 - a protector against Alzheimer's disease - BRAINPROTECT Prijenos signala u tumorima: Hh-Gli put, interakcije i potencijalne terapije Signalni put Hh-Gli evolucijski je visoko očuvan put prijenosa signala od stanične membrane do jezgre. Put ima ključnu ulogu u normalnom razvoju, a njegovo poremećeno funkcioniranje povezuje se s razvojnim deformacijama i rakom. Uloga Hh-Gli puta u manjem broju tumora tipičnih za Gorlinov sindrom proučava se već desetak godina, no tek u posljednjih nekoliko godina otkriva se njegovo sudjelovanje u tumorima pluća, dojke, prostate, probavnog trakta i drugima. Cilj predloženog projekta je potvrđivanje hipotetičkih modela poremećenog aktiviranja Hh-Gli puta u odabranim tumorima i razjašnjavanje specifičnih mehanizama aktivacije (uključujući interakcije s drugim genima), a napose istraživanje mogućnosti da se inhibiranjem signalnog puta spriječi rast tumora.više » Promijenjeno stanje DNA-metilacije u HPV-povezanim oštećenjima ProNetMel - Otkrivanje novih proteinskih interakcija kao podloga za nove pristupe liječenju melanoma čovjeka Glavni cilj ovog istraživačkog projekta je u melanomima otkriti interakcije proteina p53 s partnerskim proteinima koji su sposobni mijenjati njegovu funkciju. Posebno smo zainteresirani za moguće interakcije proteina p53 s članovima njegove obitelji kao što su p53- i p73-izoforme; s proteinima nm23, osobito nm23-H1 i nm23-H2; kao i s obitelji proteina Gli.više » Proteomska analiza tkiva u bolesnika s karcinomom prostate RapidCell - Rapid Identification of Cells in a Clinical Environment Razvoj novih pristupa molekularne i serološke dijagnostike infekcije s humanih papiloma virusima (HPV) Razvoj novih pristupa proteomskim analizama Sinteza disulfoksi i trisulfoksi derivatizacijskih analoga u svrhu nedvojbene identifikacije peptida i proteina spektrometrijom masa Stres, GABA-A receptori i mehanizmi djelovanja neuropsihofarmaka Svrha istraživanja jest unaprijediti spoznaje o mehanizmima djelovanja neuropsihofarmaka, posebice tijekom njihove kronične primjene, te primjene u uvjetima stresa. Poseban naglasak stavljen je na istraživanje benzodiazepina i antidepresiva, koji se koriste u liječenju anksioznosti, nesanice i depresije, a koji u mozgu djeluju putem GABAergičnog i serotoninskog sustava. Stoga u okviru projekta pratimo promjene u ekspresiji i funkciji GABA-A receptorain vitronakon dugotrajnog izlaganja benzodiazepinima koje može izazivati pojavu tolerancije i ovisnosti. Na eksperimentalnim životinjama također se istražuju interakcije između učinaka stresa i djelovanja benzodiazepina i antidepresiva, prvenstveno na osjetljivost prema konvulzivima, te uloga serotoninskog sustava u kontroli moždane podražljivosti.više » Sustav citokroma P450 i pojava tumora u starenju i oksidacijskom stresu Projekt se bavi istraživanjem utjecaja sustava citokroma P450 na spolne i dobne razlike u otpornosti na akutni oksidacijski stres u miševa i ljudi.više » Uloga disfunkcije lizosoma u nastanku neurodegenerativnih bolesti Koristeći stanični model lizosomalne bolesti NPC cilj ovog projekta je ispitati ulogu lizosoma i njihove disfunkcije na nakupljanje peptida amiloid-beta (Abeta) i alfa-sinukleina (a-syn), dva ključna proteina u patogenezi Alzheimerove, odnosno Parkinsonove bolesti.više » Uloga gena nm23 u karcinomu pločastog epitela usne šupljine Uloga membranskih peptidaza na tumorskim i normalnim stanicama Uloga mreže proteina p53/p73 u sarkomima mekih tkiva čovjeka Uloga različitih mehanizama odgovora stanica na terapiju oštećenjem DNA Uloga sirtuina 1 (SIRT1) u zloćudnim tumorima ne-malih stanica pluća Uspostava istraživačke infrastrukture za translacijsku medicinu i primijenjenu genomiku Uspostava istraživačke infrastrukture za translacijsku medicinu i primjenjenu genomiku s ciljem korištenja visoko-propusnih metoda temeljena na DNA čip tehnologiji za buduće aktivnosti tehnološkog transferaviše » Utjecaj kolagena modificiranog produktom lipidne peroksidacije na rast i diferencijaciju matičnih stanica karcinoma dojke Genetičko testiranje na nasljednu sklonost za rak dojke i jajnika (analiza gena BRCA1 i BRCA2) Genetičko testiranje na nasljednu sklonost za rak dojke i jajnika (analiza gena BRCA1 i BRCA2)više » Molekularna dijagnostika Laboratorij je zavodska jedinica za molekularno-genetsku analizu monogenskih oboljenja čovjeka: cističnu fibrozu, nesindromsku gluhoću i sindrom Rett.više » Molekularna dijagnostika bakterijskih i viralnih infekcija Dokazivanje prisustva bakterijskih i virusnih infekcija u uzorcima (biopsije, obrisci, eksudati)više » Molekularna dijagnostika genetskih bolesti U našem laboratoriju bavimo se dijagnosticranjem slijedećih genetskih bolesti - Fragilni X kromosom, Huntingronova bolest, Miotonična distrofija, Friedrichova ataksija, Spinalno cerebralna ataksija i Duchenne-ova mišična distrofija.više » Molekularno-genetičke analize Laboratorij za personaliziranu medicinu pruža usluge molekularno-genetičkih analiza nasljednih bolesti povezanih s povećanim rizikom oboljevanja od raka te analize farmakogenetičkih biljega.više » Određivanje mutacija u protoonkogenu ret Ova se genetička analiza provodi u oboljelih od medularnog karcinoma štitne žlijezde kako bi se isključilo/potvrdilo postojanje nasljedne mutacije. Ova se suradnja provodi s KBC "Sestre milosrdnice".više » Određivanje slijeda nukleotida (sekvenciranje DNA) i fragmentalna analiza Određivanje slijeda nukleotida (sekvenciranje DNA) i fragmentalna analizaviše » Proizvodnja laboratorijskih životinja U Pogonu laboratorijskih životinja se uzgajaju jezgre pet srođenih mišjih sojeva BALB/c, CBA/H C3Hf/Bu, C57BL/6-Ly5 i NOD, kao i jedan nesrođeni soj HSD:ICR (CD-1), te štakori (Wistar Zagreb 5HT - podsoj selekcioniran prema razini perifernog serotonina). Životinje se uzgajaju na način koji omogućava genetsku stabilnost sojeva, a životinje se nalaze pod stalnim veterinarskim nadzorom zdravstvenog stanja. Pogon laboratorijskih životinja proizvodi miševe i štakore za potrebe znanstvenika Instituta «Ruđer Bošković» kao i za vanjske naručioce.više »