http___www.kinderwunschklinik.at_hr_lijecenje_mogucnosti_lijecenja_p_icsi_1.xml
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P-ICSI Only mature and genetically acceptable sperm produce the hyaluronidase enzyme. This enzyme is necessary for fertilization and thus to achieve a pregnancy. Hyaluronidase is located in the sperm’s head and ensures that when the sperm meets the egg cell, the outer egg integument dissolves and the sperm is able to penetrate, so enabling fertilisation. With the ICSI method, a single sperm is injected into the egg using a hollow needle. In the past only the motility, appearance and shape of the sperm were considered. Through the HBA test (hyaluronidase fixation test) used in the KinderWunschKlinik, sperm are chosen according to their specific fixation properties for the ICSI procedure. All these sperm are mature and do not show DNA degradation and are therefore suitable for the ICSI fertilization. Therefore, only sperm that produce the hyaluronidase enzyme are mature and genetically sound and are consequently used in our clinic in the P-ICSI procedure. In this way we have the best chances for a positive and successful treatment.
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P-ICSI Enzim hijaluronoglukozamindazu mogu proizvoditi samo zreli i genetski besprijekorni spermatozoidi. Ovaj enzim je potreban, kako bi se ostvarila oplodnja, a time i trudnoća. Hijaluronoglukozaminidaza je smještena u glavi spermatozoida i prilikom susreta s jajnom stanicom, brine se za rastvaranje vanjskog omotača jajne stanice . Spermatozoid na taj način može prodrijeti u jajnu stanicu i dolazi do oplodnje. Kod umjetne oplodnje pomoću ICSI, pojedinačni spermatozoid se pomoću kanile ubrizgava u jajnu stanicu, pri čemu se kod ovog spermatozoida vodilo računa samo o pokretljivosti, izgledu i obliku. Jedan HBA test (test vezivanja hijaluronoglukozaminidazu) proveden na klinikama KinderWunschKliniken u Welsu ili Beču služi izboru spermatozoida. Svi spermatozoidi, koji se vezuju u okviru testa, ne pokazuju degeneracije DNK i zbog toga su prikladni za ICSI oplodnju. Time se kod P-ICSI oplodnje koriste samo oni spermatozoidi koji su genetski besprijekorni i zreli i koji proizvode enzim hijaluronoglukozamini. Tako postoje najbolji izgledi za pozitivan ishod tretmana.