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Laboratory for mass spectrometry Welcome to web pages of Laboratory for chemical kinetics and atmospheric chemistry. Area of special concern of the Laboratory members is and will be general basic research with emphasis on the study of biologically active molecules, design, synthesis and properties of organometallic complexes of biological and pharmacological significance and those that can be applied in organic synthesis, catalysis or as chromogenic and fluorescent chemosensors. We plan to measure and monitor those relevant components of the atmosphere (reactive oxygen species and reactive nitrogen species) that will allow us to study the oxidation effects of the atmosphere on the living organisms and materials. Special attention will be oriented to macromolecules – to structural analysis of peptides and proteins in complex biological mixtures. Experiments with state-of-art mass spectrometry techniques is unavoidable in achieving this goal. Thus the new sophisticated mass spectrometers which have been installed in our Laboratory this year will be used: Amazon ETD, Bruker Daltonik (LC-MS) and MALDI TOF Biotyper, Bruker Daltonik. To improve the fundamental understanding of reactivity, (biological) activity and mechanism of other classes of molecules UV photoelectron spectroscopy (PES), electronic (UV-Vis) spectrophotometry together with resident techniques including IR, NMR, EPR and X-Ray diffraction will be used. Experimental studies, combined with computational investigations provide a conceptual framework and an efficient means to obtain direct insight into reactivity patterns, the importance of electronic structure and the nature of intermediates. One category of mass spectrometry activities will be focused towards a fee-based routine service program for external users including qualitative protein identification and the identification and characterization of microorganisms Laboratory instrumentation Instruments available for the work on our research projects: - MALDI TOF mass spectrometer system for whole cell mass spectrometry - nano-LC-MS/MS system for protein primary structure research, PTM discovery and proteomics - Photoelectron Spectrometer UVG 3 - uv-vis spectrophotometer - different kind of monitors for air pollution measurements Some of the existing equipment is obviously old (e.g. photoelectron spectrometer) but is still performing satisfactorily and is being used to produce high quality research work as evidenced by the publications. Tabular view of instruments available in laboratory: Branka Bilić PhD Marko Rožman Saša Kazazić PhD Snježana Kazazić PhD Design, synthesis and properties of organic ligands and their metal complexes NMR study of Pd(II) and Pt(II) Cyclometallated Complexes of Azobenzenes East-NMR project, part of FP7 programmore »
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Laboratorij za spektrometriju masa Dobrodošli na web stranicu laboratorija za kemijsku kinetiku i atmosfersku kemiju. Prioritetno područje kojim se bave članovi Laboratorija jest i bit će opća temeljna istraživanja s naglaskom na proučavanje biološki aktivnih molekula, dizajn, sintezu i svojstva organometalnih kompleksa od biološkog i farmakološkog značaja te onih koji se mogu primijeniti u organskoj sintezi i katalizi te kao kromogeni i fluorescentni kemosenzori. Mjerit ćemo i pratiti one relevantne sastojke atmosfere (reaktivne kisikove vrste i reaktivne dušikove vrste) koji će nam omogućiti istraživanje oksidacijskog djelovanja atmosfere na žive organizme i materijale. Posebna pozornost bit će posvećena makromolekulama - strukturnoj analizi peptida i proteina u složenim biološkim smjesama. U tu ćemo svrhu rabiti nove sofisticirane spektrometre masa koji su ove godine instalirani u našem Laboratoriju: AmaZon ETD, Bruker Daltonik (LC-MS) i MALDI TOF Biotyper, Bruker Daltonik. Pri provedbi ostalih istraživanja primjenjivat ćemo fotoelektronsku spektroskopiju (PES), elektronsku (UV-Vis) spektrofotometriju te na Institutu dostupne spektroskopske metode IR, NMR, EPR, metode rendgenske difrakcije te kvantno-kemijske račune. Eksperimentalna proučavanja dopunjena računima važna su jer predstavljaju konceptualni okvir i efikasan način dobivanja izravnog uvida u pravilnost kojom se mijenja reaktivnost, važnost elektronske strukture i priroda međuprodukata. Spomenimo da će spektrometri masa biti uključeni i u rutinske servisne usluge vezane uz kvalitativnu identifikaciju proteina te karakterizaciju mikroorganizama . Instrumentacija laboratorija Instrumenti na raspolaganju za rad na našim istraživačkim projektima: - MALDI TOF maseni spektrometar sustav za masenu spektrometriju cijelih stanica - nano-LC-MS/MS sustav za istraživanje primarne strukture proteina, PTM discovery i proteomiku - UV fotoelektronski spektrometar UVG 3 - UV-VIS spektrofotometar - Razne vrste monitora za mjerenje zagađenja zraka Neki instrumenti u postojećoj opremi su očigledno stari (npr. fotoelektronski spektrometar) no i dalje rade sa zadovoljavajućim performansama i pomoću njih se izvodi visokokvalitetan znanstveni rad što je evidentno u publikacijama. Tabelarni prikaz instrumenata u uporabi u laboratoriju: Branka Bilić dr.sc. Marko Rožman dr.sc. Saša Kazazić dr. sc. Snježana Kazazić dr. sc. Dizajn, sinteza i svojstva organskih liganada i njihovih metalnih kompleksa Istraživanje Pd(II) i Pt(II) ciklometaliranih kompleksa azobenzena NMR spektroskopijom East-NMR projekt unutar FP7 programaviše »