http___www.irb.hr_Istrazivanja_Zavodi_Centar-za-istrazivanje-mora_Laboratorij-za-morsku-mikrobnu-ekologiju_Otkrivanje-sastava-i-raznolikosti-pikofitoplanktona-u-obalnim-vodama-sjevernog-Jadrana_1.xml
1.1 2.1 2.2 2.3 2.4 2.5 3.1 3.2 3.3 3.4 4.1 5.1 5.2 5.3 6.1 6.2 7.1 9.1 11.1 12.1 14.1
Uncovering composition and diversity of marine picophytoplankton in northern Adriatic coastal waters Picophytoplankton (cells < 3 µm) contribute significantly to marine plankton biomass and productivity. Recent molecular studies have brought to light their wide diversity. Our knowledge of the diversity of picoplankton is very limited for the Adriatic Sea, so developing methods for assessing and monitoring their ecology and diversity is essential for understanding their contribution in food web dynamics and biochemical cycles. The lack of data is due to technical difficulties in identifying and classifying picoplanktonic organisms with light microscopy, which clarifies the need for new efficient tools in the studying of these small organisms. Among the molecular methods that have been used so far DGGE has been proved as the best compromise between the number of samples and obtained information. Results: The main idea of this project was to test and validate DGGE as a method that allows routine assessment of picophytoplankton diversity in the Adriatic Sea. Our strategy to meet this objective was encapsulated in my 3 month stay in the hosting lab at the ICM Institute. In that period I was able to learn the preparation of samples consisting of PCR amplification of 16S and 18S rRNA; preparation of DGGE gels, cutting gels for sequencing and interpretation of results. Some of the results were already presented at the hosting Institute during my stay (November 23rd, 2010 at 10 h, hall S47a), which provided me an excellent opportunity for discussion with eminent experts from this field. After the final analysis, the results will be discussed in my doctoral thesis, scientific publications and public lectures. I must admit that the cooperation with the hosting organization was much better than I was expecting. Their hospitality and good will to help in any moment I needed assistance with the processing of samples or working with instruments or interpretation of results was really great. That level of healthy working spirit I must say is pretty rare. I have gained great experience while working in this lab and I think that the main benefit is not only my new skills gained from outstanding experienced researchers but as well the establishment of future collaboration. Establishing working networks worldwide is surely one of the most important parts in increasing international competitiveness of national scientific centers like the Center for marine research. - Project leader / principal investigator - Associates Dr. Carlos Pedrós-Alió - Notes Partner Institution/Company:Mediterranean Marine and Environmental Research Centre (CMIMA), Institute of Marine Sciences (ICM), Department of Marine Biology and Oceanography
1.1 2.1 2.2 2.3 2.4 2.5 3.1 3.2 3.3 3.4 4.1 5.1 5.2 6.1 6.2 6.3 7.1 9.1 11.1 12.1 14.1
Otkrivanje sastava i raznolikosti pikofitoplanktona u obalnim vodama sjevernog Jadrana Pikofitoplankton (stanice veličine do 3 µm) znatno doprinose biomasi i produktivnosti morskog planktona. Primjenom molekularnih metoda u polju mikrobne ekologije došlo se do saznanja o njihovoj raznolikosti. O pikofitoplanktonu u Jadranskom moru se zna jako malo, tako da je razvoj novih metoda za procjenu i praćenje njihove ekologije i raznolikosti ključno za razumijevanje njihovog potencijala i doprinosa u dinamici hranidbenog lanca kao i biokemijskih ciklusa u morskom sustavu. Nedostatak podataka posljedica je tehničkih problema u prepoznavanju i klasificiranju pikoplanktonskih organizama svjetlosnim mikroskopom, što pojašnjava potrebu za novim učinkovitijim alatima za njihovo proučavanje. Među molekularnim metodama korištenim do sad u proučavanju ove najmanje jedinice planktona elektoroforeza u gradijentu denaturirajućeg gela (DGGE) se pokazala kao najbolji kompromis između broja uzoraka i dobivenih informacija. Glavna ideja ovog projekta je bila testirati i utvrditi DGGE kao metodu koja omogućuje rutinske procjene raznolikosti pikofitoplanktona u Jadranskom moru. Da bih postigla navedeno boravila sam 3 mjeseca u laboratoriju ICM instituta. U tom razdoblju sam naučila: pripremu uzoraka za DGGE metodu, koja se sastojala od lančane reakcije polimerazom (PCR) 16S rRNA i 18S rRNA, pripreme DGGE gelova, izrezivanja zanimljivih vrpci iz gelova, te intepretacije dobivenih sekvenci. Neki od rezultata već su prezentirani na ICM Institutu tijekom mog boravka (23. studenog 2010., u 10 h, Predavaona S47a), kada sam imala izvrsnu priliku za diskusiju rezultata s eminentnim stručnjacima iz podučja morske mikrobne ekologije. Nakon konačne analize svih rezultata, navedeni će biti interpretirani u mojoj doktorskoj disertaciji, znanstvenim radovima, te prezentirani na kongresima. Suradnja s ICM Institutom koji mi je bio domaćin je moram priznati bila puno bolja nego što sam očekivala. Njihova gostoljubivost i dobra volja za pomoć pri obradi uzoraka, radu s instrumentima i tumačenju rezultata je bila stvarno velika. Primjena DGGE metode će dati bolji uvid u ekologiju pikofitoplanktona kao i poboljšati našu interpretaciju okoliša i ekoloških procesa. No doprinos ovog posjeta nisu samo usvajanje i stjecanje novih saznanja i vještina od izuzetnih znanstvenika već i uspostava buduće suradnje. Uspostava suradnje je zasigurno jedan od najvažnijih načina za povećanje međunarodne konkurentnosti nacionalnih znanstvenih centara kao što je Centar za istraživanje mora. - Glavni istraživač / voditelj - Ostali suradnici Carlos Pedrós-Alió - Napomena Partnerska institucija:Mediterranean Marine and Environmental Research Centre (CMIMA), Institute of Marine Sciences (ICM), Department of Marine Biology and Oceanography