http___www.irb.hr_Istrazivanja_Zavodi_Zavod-za-istrazivanje-mora-i-okolisa_Laboratorij-za-biogeokemiju-mora-i-atmosfere_1.xml
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Algal cell biophysical properties as markers for environmental stress in aquatic systems Major challenges face the aquatic systems due to different human activities, which may cause substantial damage to the economy of the region (fisheries, tourism). Our common regional challenge is focused on analyzing pressure in aquatic environment and requires transnational action as recognized in EU Biodiversity Strategy 2020. Single-celled algae play a fundamental role in the aquatic ecosystem and they are very sensitive to environmental changes. In addition to their ecological roles (oxygen producers, food web), they are economically important (biofuel, human food, pharmaceutical and industrial products). Deep understanding of algal cell physicochemical response is essential to predict how the whole ecosystem may be affected by human activities. Physicochemical and structural characterizations of single algal cell surface are of key importance to interpret, rationalize and predict the behavior and fate of the cell under stress in vivo. We aim to examine the in vitro algal cell response under specific stress conditions (variation of salinity and heavy metals). We will look into cell surface mechanical properties (linked to adhesion dynamics and motility), as well as the membrane protein expression and cell autofluorescence. Such properties of soft and motile cells are hard to access while keeping cells viable and the studies to date are limited. Innovative comprehensive approach with state-of-the-art biophysical methods such as atomic force microscopy, nanoindentation, electrochemistry, spectroscopy and modelling will be employed. Our solution necessitates regional collaboration to gain immediate access to the specific equipment and complementary expertise. Project is financed by International Visegrad Fund, Bratislava, Slovakia. Partners: Faculty of Mechanical Engineering, Czech Technical University in Prague, Prague, Czech Republic, Prague, Czech Republic (Dr. Josef Sepitka) Faculty of Science and Informatics, University of Szeged, Szeged, Hungary (Dr. Bela Gyurcsik) Faculty of Natural Sciences, University of Ss. Cyril and Methodius, Trnava, Slovakia (Dr. Alzbeta Marcek Chorvatova) Announcment of project activity: Kickoff meeting 21.09.-22.09.2017. Ruđer Bošković Institute, Zagreb, Croatia Scientific regional exchanges Zuzana Pavlinska, M. Sc. student, from the Faculty of Natural Sciences at University of Ss Cyril and Methodius in Trnava, Slovakia will visit the Laboratory of nanoindentation at the Faculty of Mechanical Engineering, Czech Technical University in Prague, Czech Republic. Host: Dr. J. Sepitka Work plan: evaluation of mechanical properties of algal cells at polymeric surfaces Ing. Katarina Mendova, PhD student from the Faculty of Mechanical Engineering at Czech Technical University in Prague, Czech Republic will visit the Faculty of Natural Sciences at University Ss. Cyril and Methodius in Trnava, Slovakia. Host: Dr. A. Marcek Chorvatova Work plan: evaluation of endogenous fluorescence of algae and its changes in different experimental conditions. - Glavni istraživač / voditelj
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Laboratorij za biogeokemiju mora i atmosfere Misija i vizija Laboratorija za biogeokemiju mora i atmosfere uključuje temeljna i primjenjena interdisciplinarna istraživanja unutar znanosti o moru i o atmosferi te obrazovanje mladih znanstvenika. Voditelj laboratorija dr.sc. Blaženka Gašparović Glavni fokus Laboratorija za biogeokemiju mora i atmosfere je organska tvar koja je od važnosti za biogeokemijski ciklus ugljika a time i za klimatske promjene. S obzirom da je u zadnjih dva stoljeća ozbiljno narušen globalni ciklus ugljika intenzivnom ljudskom aktivnošću, cilj je istraživati: (i) reakcije fitoplanktona na okolišni stres (temperatura, dostupnost nutrijenta, zagađivala) kao ključne karike u trofičkom lancu, (ii) staničnu i vanstaničnu organsku tvar (lipidi, morski gelovi, polisaharidi, proteini) što je važno za određivanje porijekla i procesa organske tvari; definiranje njihove organizacije i strukture na molekularnoj skali, te mehaničkih svojstava (iii) površinska svojstva stanica na nano i mikrometarskoj skali koja su teško dostupna drugim tehnikama ali su presudna za razumjevanje dinamike međupovršinskih procesa: biotskih i abiotskih, (iv) raspoloživost organske tvari za metale i nanočestice, (v) veličinsku distribuciju, svojstva te fotokemijske procese organske tvari atmosferskih aerosola s naglaskom na nitro-aromatskim spojevima te njihove interakcije s okolišnim mikro konstituentima, (vi) porijeklo, (trans)formacije te procese izmjene organske tvari na granici faza morska voda/atmosfera putem specifičnih organskih spojeva kao što su nitro-aromatski spojevi, te distribuciju istih u vodenom stupcu. Blaženka Gašparović dr.sc. Jelena Godrijan dr.sc. Nadica Ivošević DeNardis Sanja Frka Milosavljević dr.sc. Slađana Strmečki Kos dr.sc. Tea Mišić Radić dr.sc. Algal cell biophysical properties as markers for environmental stress in aquatic systems Major challenges face the aquatic systems due to different human activities, which may cause substantial damage to the economy of the region (fisheries, tourism). Our common regional challenge is focused on analyzing pressure in aquatic environment and requires transnational action as recognized in EU Biodiversity Strategy 2020.više » Estimating the role of marine biogenic organosulfur compunds in the formation and properties of atmospheric organic aerosols Hrvatsko-Slovenska znanstveno tehnološka suradnja, 2016-2017, voditelj: S. Frka Milosavljevićviše » FP7 Programme, Sensitive MeAsuRmenT, detection and identification of engineered NANO particles in complex matrices Lyons, suradnik: T. Mišić Radićviše » HRZZ: New methodological approach in biogeochemical studies of trace metal speciation in coastal aquatic ecosystems HRZZ: Utjecaj okolišnog stresa na pojavnost i međudjelovanje biološki važnih organskih molekula i mikronutrijenata u morskom ekosustavu HRZZ: Životne strategije fitoplanktona u sjevernom Jadranu, Marie-Curie co-fund project: Chemical nature of size segregated water soluble organic aerosols and their nitro-aromatic constituents AFM usluge analize Vizualizacija i karakterizacija površina i površinskih procesa na nanoskali primjenom mikroskopije atomskih sila.više » TOC analizator Analizator za određivanje ukupnog, otopljenog i partikularnog organskog ugljika u prirodnim krutim i tekućim uzorcima.više »