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Laboratory for Molecular and Cellular Biology The Laboratory for Molecular and Cellular Biology has two research groups that are active at different areas of eukaryotic molecular and cell biology. Model systems are yeast and mammalian cells. The scientific aims of our lab include mechanisms of cell growth control and gene activity. Group Ivica Rubelj: Our main research interest is telomere structure and function and their role in control of normal and immortal cell growth. Telomeres are repetitive sequences at the ends of eukaryotic chromosomes and they play a crucial role in protection of genome integrity, cell growth control and immortalization. Through interactions with several proteins they shape chromosome ends into so called telomere loops (t-loop) in order to prevent possible modifications by DNA repair mechanisms. In normal cells they continuously shorten with each division which ultimately leads to cell senescence. Besides gradual telomere shortening there is also abrupt telomere shortening which we proposed as a mechanism that underlies the sudden and stochastic appearance of senescent cells in culture. Group Mary Sopta: Regulation of gene expression at the level of transcription is a fundamentally important process in the differentiation of different cell types and the development of organisms. Gene transcription by RNA polymerase II in eukaryotes involves a plethora of auxiliary protein factors which are required for the processes of transcription initiation, elongation and termination, each of which represents a potential regulatory point. Our group is interested in various aspects of transcriptional regulation in yeast as well as the role of transcription factors, in processes such as DNA repair. We are currently studying the role of Cdk8 in Gal4 dependent transcription as well as the role of the TFIIF small subunit (Tfg3) in transcription and DNA repair. Ivica Rubelj Josipa Nemet dr.sc. Lucia Nanić Marija-Mary Sopta Dr.sc. Nikolina Škrobot Vidaček Nikolina Vidan Functional characterization of the fungal specific protein Taf14 in S.cerevisiae and C. albicans Invasive fungal infections have become an important cause of mortality in immunocompromised and severely ill patients, in both the developing world and western countries.more »
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Laboratorij za molekularnu i staničnu biologiju Laboratorij se bavi istraživanjima molekularne i stanične biologije eukariota. Modelni sistemi su stanice kvasca i sisavaca. Istraživanja obuhvaćaju mehanizme kontrole staničnog rasta i aktivnosti gena. Laboratorij sačinjavaju dvije istraživačke grupe. Grupa I. Rubelj: Naš glavni interes je istraživanje strukture i funkcije telomera u kontroli normalnog i imortalnog staničnog rasta. Telomere su ponavljajuće sekvence koje se nalaze na krajevima eukariotskih kromosoma i imaju presudnu ulogu u zaštiti i očuvanju integriteta genoma. One sudjeluju u nekim od najvažnijih staničnih funkcija kao što su dioba i kontrola normalnog rasta te održavanju imortalnog stanja tumorskih stanica i jednostaničnih eukariota. Presudnu ulogu imaju u kontroli staničnog starenja te tranziciji normalne stanice u tumorsku. Stoga je u proteklom razdoblju naglasak u mojim istraživanjima stavljen upravo na strukturu i funkciju telomera, istraživanja mehanizama njihovog održavanja (dinamika skraćivanja i/ili produžavanja) u raznim sistemima normalnih odnosno imortalnih stanica, mogućih strukturnih promjena tijekom tranzicije u imortalno stanje, odnosno indukcije staničnog starenja. Grupa M. Sopta: Regulacija ekspresije gena na razini ekpresije je iznimno važan proces prilikom diferencijacije različitih tipova stanica i razvoja organizama. Transkripcija gena sa RNA polimerazom II kod eukariota uključuje proteinske faktore potrebne za procese inicijacije transkripcije, elongacije i terminacije, gdje svaki od njih predstavlja potencijalnu regulacijsku točku.Naša grupa je usmjerena na različite aspekte transkripcijske regulacije kod kvasca, kao i na ulogu transkripcijskih faktora u procesima popravka DNA. Trenutno proučavamo ulogu Cdk8 kod transkripcije ovisne o Gal4, kao i ulogu male podjedinice TFIIF (Tfg3) u transkripciji i popravku DNA. Ivica Rubelj dr. sc. Josipa Nemet dr.sc. Lucia Nanić Marija-Mary Sopta Dr.sc. Functional characterization of the fungal specific protein Taf14 in S.cerevisiae and C. albicans Invasive fungal infections have become an important cause of mortality in immunocompromised and severely ill patients, in both the developing world and western countries.više »