Production and properties of nanoelectrospray emitters used in Fourier transform ion cyclotron resonance mass spectrometry: Implications for determination of association constants for noncovalent complexes
UNSPECIFIED. (2004) Production and properties of nanoelectrospray emitters used in Fourier transform ion cyclotron resonance mass spectrometry: Implications for determination of association constants for noncovalent complexes. ANALYTICAL CHEMISTRY, 76 (17). pp. 5172-5179. ISSN 0003-2700Full text not available from this repository.
Official URL: http://dx.doi.org/10.1021/ac049569z
Electrospray ionization (ESI) is extensively used in the analysis of biological compounds; yet some fundamental properties of this technique are not completely understood. It is widely recognized that care should be exercised when noncovalent complexes are being studied by ESI, since weak noncovalent binding can be broken or formed during the desolvation process. In the present work, spectra from the noncovalent complex, vancomycin/diacetyl-L-lysyl-D-alanyl-D-alanine, obtained from ESI and from nanoelectrospray ionization (nanoESI), have been compared. The results indicated that the milder desolvation conditions arising as a result of the smaller sizes of droplets produced in the nanoESI source attenuated effects upon weak bonds in the desolvation process. The association constant values calculated from the relative peak intensities suggest that, when using ESI, the analyzed noncovalent complex dissociated in the condensed phase during the spraying process. The influences of experimental parameters such as tip diameter and coating for nanoESI needles were investigated. Principal component analysis, a multivariate analysis method, was applied to achieve a better evaluation of the spectra obtained using different needle diameters and coatings for the analysis of the noncovalent complex vancomycin/ diacetyl-L-lysyl-D-alanyl-D-alanine. It was found that 2-mum tip diameter resulted in more reproducible spectra than the larger tip diameters tested (6-20 mum).
|Item Type:||Journal Article|
|Subjects:||Q Science > QD Chemistry|
|Journal or Publication Title:||ANALYTICAL CHEMISTRY|
|Publisher:||AMER CHEMICAL SOC|
|Official Date:||1 September 2004|
|Number of Pages:||8|
|Page Range:||pp. 5172-5179|
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