@Test
public void test1D66() throws Exception {

    String filename = "data/pdb/1D66.pdb";
    InputStream ins = this.getClass().getClassLoader().getResourceAsStream(filename);
    ISimpleChemObjectReader reader = new PDBReader(ins);
    Assert.assertNotNull(reader);
    IChemFile chemFile = (IChemFile) reader.read(new ChemFile());
    Assert.assertNotNull(chemFile);
    Assert.assertEquals(1, chemFile.getChemSequenceCount());
    org.openscience.cdk.interfaces.IChemSequence seq = chemFile.getChemSequence(0);
    Assert.assertNotNull(seq);
    Assert.assertEquals(1, seq.getChemModelCount());
    IChemModel model = seq.getChemModel(0);
    Assert.assertNotNull(model);
    Assert.assertEquals(1, model.getMoleculeSet().getAtomContainerCount());
    IAtomContainer container = model.getMoleculeSet().getAtomContainer(0);
    Assert.assertTrue(container instanceof IBioPolymer);
    IBioPolymer polymer = (IBioPolymer) container;
    Assert.assertTrue(polymer instanceof PDBPolymer);
    PDBPolymer pdb = (PDBPolymer) polymer;
    Assert.assertEquals(4, pdb.getStrandCount());
    Assert.assertTrue(polymer.getStrandNames().contains("D"));
    Assert.assertTrue("Strand D is not a PDBStrand", polymer.getStrand("D") instanceof PDBStrand);
    Assert.assertTrue(polymer.getStrandNames().contains("E"));
    Assert.assertTrue("Strand E is not a PDBStrand", polymer.getStrand("E") instanceof PDBStrand);
    Assert.assertTrue(polymer.getStrandNames().contains("A"));
    Assert.assertTrue("Strand A is not a PDBStrand", polymer.getStrand("A") instanceof PDBStrand);
    Assert.assertTrue(polymer.getStrandNames().contains("B"));
    Assert.assertTrue("Strand B is not a PDBStrand", polymer.getStrand("B") instanceof PDBStrand);
    //Check to pick up all 4 strands
    Assert.assertEquals(polymer.getStrands().size(), 4);
    //The following check is to see that the first monomers in a strand
    //can be accessed consecutively
    //i.e. their resSeq numbering follows that in the File
    //Strand A
    PDBStrand strandA = (PDBStrand) polymer.getStrand("A");
    Collection<String> lst = strandA.getMonomerNamesInSequentialOrder();
    //Should be 57 monomers in strand A
    Assert.assertEquals(57, lst.size());
    Iterator<String> lstIter = lst.iterator();
    String monomer1 = lstIter.next();
    IMonomer mono1 = strandA.getMonomer(monomer1);
    Assert.assertNotNull(mono1);
    Assert.assertNotNull(mono1.getMonomerName());
    Assert.assertTrue("Monomer is not a PDBMonomer", mono1 instanceof PDBMonomer);
    PDBMonomer pdbMonomer = (PDBMonomer) mono1;
    Assert.assertEquals("A", pdbMonomer.getChainID());
    Assert.assertEquals("8", pdbMonomer.getResSeq());
    monomer1 = lstIter.next();
    mono1 = strandA.getMonomer(monomer1);
    pdbMonomer = (PDBMonomer) mono1;
    Assert.assertEquals("A", pdbMonomer.getChainID());
    Assert.assertEquals("9", pdbMonomer.getResSeq());
    monomer1 = lstIter.next();
    mono1 = strandA.getMonomer(monomer1);
    pdbMonomer = (PDBMonomer) mono1;
    Assert.assertEquals("A", pdbMonomer.getChainID());
    Assert.assertEquals("10", pdbMonomer.getResSeq());
    //Strand B
    PDBStrand strandB = (PDBStrand) polymer.getStrand("B");
    lst = strandB.getMonomerNamesInSequentialOrder();
    //Should be 57 monomers in strand B
    Assert.assertEquals(57, lst.size());
    lstIter = lst.iterator();
    monomer1 = lstIter.next();
    mono1 = strandB.getMonomer(monomer1);
    Assert.assertNotNull(mono1);
    Assert.assertNotNull(mono1.getMonomerName());
    Assert.assertTrue("Monomer is not a PDBMonomer", mono1 instanceof PDBMonomer);
    pdbMonomer = (PDBMonomer) mono1;
    Assert.assertEquals("B", pdbMonomer.getChainID());
    Assert.assertEquals("8", pdbMonomer.getResSeq());
    monomer1 = lstIter.next();
    mono1 = strandB.getMonomer(monomer1);
    pdbMonomer = (PDBMonomer) mono1;
    Assert.assertEquals("B", pdbMonomer.getChainID());
    Assert.assertEquals("9", pdbMonomer.getResSeq());
    monomer1 = lstIter.next();
    mono1 = strandB.getMonomer(monomer1);
    pdbMonomer = (PDBMonomer) mono1;
    Assert.assertEquals("B", pdbMonomer.getChainID());
    Assert.assertEquals("10", pdbMonomer.getResSeq());
    //Strand E
    PDBStrand strandE = (PDBStrand) polymer.getStrand("E");
    lst = strandE.getMonomerNamesInSequentialOrder();
    //Should be 19 monomers in strand E
    Assert.assertEquals(19, lst.size());
    lstIter = lst.iterator();
    monomer1 = lstIter.next();
    mono1 = strandE.getMonomer(monomer1);
    Assert.assertNotNull(mono1);
    Assert.assertNotNull(mono1.getMonomerName());
    Assert.assertTrue("Monomer is not a PDBMonomer", mono1 instanceof PDBMonomer);
    pdbMonomer = (PDBMonomer) mono1;
    Assert.assertEquals("E", pdbMonomer.getChainID());
    Assert.assertEquals("20", pdbMonomer.getResSeq());
    monomer1 = lstIter.next();
    mono1 = strandE.getMonomer(monomer1);
    pdbMonomer = (PDBMonomer) mono1;
    Assert.assertEquals("E", pdbMonomer.getChainID());
    Assert.assertEquals("21", pdbMonomer.getResSeq());
    monomer1 = lstIter.next();
    mono1 = strandE.getMonomer(monomer1);
    pdbMonomer = (PDBMonomer) mono1;
    Assert.assertEquals("E", pdbMonomer.getChainID());
    Assert.assertEquals("22", pdbMonomer.getResSeq());
    //Chain D should be 1,2,3...19
    PDBStrand strandD = (PDBStrand) polymer.getStrand("D");
    lst = strandD.getMonomerNamesInSequentialOrder();
    //Should be 19 monomers in strand D
    Assert.assertEquals(19, lst.size());
    lstIter = lst.iterator();
    monomer1 = lstIter.next();
    mono1 = strandD.getMonomer(monomer1);
    Assert.assertNotNull(mono1);
    Assert.assertNotNull(mono1.getMonomerName());
    Assert.assertTrue("Monomer is not a PDBMonomer", mono1 instanceof PDBMonomer);
    pdbMonomer = (PDBMonomer) mono1;
    Assert.assertEquals("D", pdbMonomer.getChainID());
    Assert.assertEquals("1", pdbMonomer.getResSeq());
    monomer1 = lstIter.next();
    mono1 = strandD.getMonomer(monomer1);
    pdbMonomer = (PDBMonomer) mono1;
    Assert.assertEquals("D", pdbMonomer.getChainID());
    Assert.assertEquals("2", pdbMonomer.getResSeq());
    monomer1 = lstIter.next();
    mono1 = strandD.getMonomer(monomer1);
    pdbMonomer = (PDBMonomer) mono1;
    Assert.assertEquals("D", pdbMonomer.getChainID());
    Assert.assertEquals("3", pdbMonomer.getResSeq());
    // PDB Structures validation
    //Should have 6 helices
    Assert.assertEquals(6, pdb.getStructures().size());
}