In 1537, during the reign of João III, the university moved definitively to Coimbra, where it was installed in the Alcaçova Palace which was subsequently purchased from the Royal Family in 1597.
Em 1537, durante o reinado de D. João III, a universidade foi transferida de maneira definitiva para Coimbra, onde foi instalada no Palácio de Alcáçova, tendo sido adquirida pela Família Real em 1597.