Methylphosphonate oligonucleotide sythesis refers to synthesis of non-ionic nucleic acid analogs. This modification is most commonly used to protect the termini of olionucleotides from enzymatic degration, by replacing negatively charged phosphodiester bonds with this neutally charged backgones. These analogs are hydrophobic and are taken up by mammalian cells via passive diffusion. They have been found to be effective antisense reagents, capable of specifically controlling viral or cellular gene expression at the mRNA level.
Bio-Synthesis offers high-purity Methylphosphonates oligonucleotides at an exceptionally competitive price. We can have all the diester bonds substituted or only incorporate partial methylphosphonate linkages between specific residues, leaving the other linkages as normal phosphodiesters as chimeric designs to increase solubility problems.
DNA methylation is a type of chemical modification of DNA that can be inherited and subsequently removed without changing the original DNA sequence.
DNA Methylation in Humans
In humans, the process of DNA methylation is carried out by three enzymes, DNA methyltransferase 1, 3a, and 3b (DNMT1, DNMT3a, DNMT3b). It is thought that DNMT3a and DNMT3b are the de novo methyltransferases that set up DNA methylation patterns early in development.