Local population structure and context-dependent isolation by distance in a large coastal shark
Jimiane L. Ashe, Kevin A. Feldheim, Andrew T. Fields, Eric A. Reyier, Edward J. Brooks, Martin T. O'Connell, Gregory Skomal, Samuel H. Gruber, Demian D. Chapman
Marine Biology and Fisheries
Genetic diversity, population genetic structure and isolation by distance (IBD) were assessed in a viviparous coastal shark (the lemon shark Negaprion brevirostris) across 8 western Atlantic samples spaced between ∼150 and 7000 km apart. Juveniles (N = 325) were sequenced at 2 mitochondrial loci (1729 bp) and typed at 9 nuclear encoded microsatellite loci. Analysis of mitochondrial sequences revealed higher diversity at low-latitude island samples compared to highlatitude continental samples, consistent with an equatorial center-of-origin for this species. There were 5 distinct groups across our sampling areas (Brazil, Louisiana, Cape Canaveral, Gullivan Bay and the Florida Keys/Bahamas/Virgin Islands; pairwise ℙST = 0.07-0.87) and all but one pair of the 8 samples also exhibited significantly different haplotype frequencies (pairwise FST = 0.10-0.51). Bayesian analysis indicated that the Brazil and Louisiana samples were generally isolated from the others, but most of the rest were diverged although still connected or recently connected by migration. In contrast, structure was only detected between the most distant sample (Brazil) and all of the others using the microsatellite markers (pairwise FST = 0.03-0.06). There was a significant pattern of IBD for all markers and measures of genetic differentiation (r2 = 0.65-0.81, p < 0.05- 0.01), but not after removing the Brazil sample. There was evidence that glacial and post-glacial historical processes and sex-specific differences in philopatry affected IBD. Because of the relatively fine-scale population structure of this and other large coastal shark species more attention should be paid to local processes in the conservation and fisheries management of these species.
Marine Ecology Progress Series
https://doi.org/10.3354/meps11069
Microsatellites
Population structure
10.3354/meps11069
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sharks Agriculture & Biology 100%
shark Earth & Environmental Sciences 97%
population structure Earth & Environmental Sciences 95%
marker Earth & Environmental Sciences 49%
genetic isolation Earth & Environmental Sciences 46%
genetic differentiation Earth & Environmental Sciences 37%
Negaprion brevirostris Agriculture & Biology 37%
genetic structure Earth & Environmental Sciences 34%
Ashe, J. L., Feldheim, K. A., Fields, A. T., Reyier, E. A., Brooks, E. J., O'Connell, M. T., Skomal, G., Gruber, S. H., & Chapman, D. D. (2015). Local population structure and context-dependent isolation by distance in a large coastal shark. Marine Ecology Progress Series, 520, 203-216. https://doi.org/10.3354/meps11069
Local population structure and context-dependent isolation by distance in a large coastal shark. / Ashe, Jimiane L.; Feldheim, Kevin A.; Fields, Andrew T.; Reyier, Eric A.; Brooks, Edward J.; O'Connell, Martin T.; Skomal, Gregory; Gruber, Samuel H.; Chapman, Demian D.
In: Marine Ecology Progress Series, Vol. 520, 03.02.2015, p. 203-216.
Ashe, JL, Feldheim, KA, Fields, AT, Reyier, EA, Brooks, EJ, O'Connell, MT, Skomal, G, Gruber, SH & Chapman, DD 2015, 'Local population structure and context-dependent isolation by distance in a large coastal shark', Marine Ecology Progress Series, vol. 520, pp. 203-216. https://doi.org/10.3354/meps11069
Ashe JL, Feldheim KA, Fields AT, Reyier EA, Brooks EJ, O'Connell MT et al. Local population structure and context-dependent isolation by distance in a large coastal shark. Marine Ecology Progress Series. 2015 Feb 3;520:203-216. https://doi.org/10.3354/meps11069
Ashe, Jimiane L. ; Feldheim, Kevin A. ; Fields, Andrew T. ; Reyier, Eric A. ; Brooks, Edward J. ; O'Connell, Martin T. ; Skomal, Gregory ; Gruber, Samuel H. ; Chapman, Demian D. / Local population structure and context-dependent isolation by distance in a large coastal shark. In: Marine Ecology Progress Series. 2015 ; Vol. 520. pp. 203-216.
@article{7c5039ff58d543a9bb0c0f71c468f51b,
title = "Local population structure and context-dependent isolation by distance in a large coastal shark",
abstract = "Genetic diversity, population genetic structure and isolation by distance (IBD) were assessed in a viviparous coastal shark (the lemon shark Negaprion brevirostris) across 8 western Atlantic samples spaced between ∼150 and 7000 km apart. Juveniles (N = 325) were sequenced at 2 mitochondrial loci (1729 bp) and typed at 9 nuclear encoded microsatellite loci. Analysis of mitochondrial sequences revealed higher diversity at low-latitude island samples compared to highlatitude continental samples, consistent with an equatorial center-of-origin for this species. There were 5 distinct groups across our sampling areas (Brazil, Louisiana, Cape Canaveral, Gullivan Bay and the Florida Keys/Bahamas/Virgin Islands; pairwise ℙST = 0.07-0.87) and all but one pair of the 8 samples also exhibited significantly different haplotype frequencies (pairwise FST = 0.10-0.51). Bayesian analysis indicated that the Brazil and Louisiana samples were generally isolated from the others, but most of the rest were diverged although still connected or recently connected by migration. In contrast, structure was only detected between the most distant sample (Brazil) and all of the others using the microsatellite markers (pairwise FST = 0.03-0.06). There was a significant pattern of IBD for all markers and measures of genetic differentiation (r2 = 0.65-0.81, p < 0.05- 0.01), but not after removing the Brazil sample. There was evidence that glacial and post-glacial historical processes and sex-specific differences in philopatry affected IBD. Because of the relatively fine-scale population structure of this and other large coastal shark species more attention should be paid to local processes in the conservation and fisheries management of these species.",
keywords = "Microsatellites, Mitochondrial DNA, Phylogeography, Population structure",
author = "Ashe, {Jimiane L.} and Feldheim, {Kevin A.} and Fields, {Andrew T.} and Reyier, {Eric A.} and Brooks, {Edward J.} and O'Connell, {Martin T.} and Gregory Skomal and Gruber, {Samuel H.} and Chapman, {Demian D.}",
note = "Publisher Copyright: {\textcopyright} Inter-Research 2015.",
doi = "10.3354/meps11069",
journal = "Marine Ecology - Progress Series",
publisher = "Inter-Research",
T1 - Local population structure and context-dependent isolation by distance in a large coastal shark
AU - Ashe, Jimiane L.
AU - Feldheim, Kevin A.
AU - Fields, Andrew T.
AU - Reyier, Eric A.
AU - Brooks, Edward J.
AU - O'Connell, Martin T.
AU - Skomal, Gregory
AU - Gruber, Samuel H.
AU - Chapman, Demian D.
N1 - Publisher Copyright: © Inter-Research 2015.
N2 - Genetic diversity, population genetic structure and isolation by distance (IBD) were assessed in a viviparous coastal shark (the lemon shark Negaprion brevirostris) across 8 western Atlantic samples spaced between ∼150 and 7000 km apart. Juveniles (N = 325) were sequenced at 2 mitochondrial loci (1729 bp) and typed at 9 nuclear encoded microsatellite loci. Analysis of mitochondrial sequences revealed higher diversity at low-latitude island samples compared to highlatitude continental samples, consistent with an equatorial center-of-origin for this species. There were 5 distinct groups across our sampling areas (Brazil, Louisiana, Cape Canaveral, Gullivan Bay and the Florida Keys/Bahamas/Virgin Islands; pairwise ℙST = 0.07-0.87) and all but one pair of the 8 samples also exhibited significantly different haplotype frequencies (pairwise FST = 0.10-0.51). Bayesian analysis indicated that the Brazil and Louisiana samples were generally isolated from the others, but most of the rest were diverged although still connected or recently connected by migration. In contrast, structure was only detected between the most distant sample (Brazil) and all of the others using the microsatellite markers (pairwise FST = 0.03-0.06). There was a significant pattern of IBD for all markers and measures of genetic differentiation (r2 = 0.65-0.81, p < 0.05- 0.01), but not after removing the Brazil sample. There was evidence that glacial and post-glacial historical processes and sex-specific differences in philopatry affected IBD. Because of the relatively fine-scale population structure of this and other large coastal shark species more attention should be paid to local processes in the conservation and fisheries management of these species.
AB - Genetic diversity, population genetic structure and isolation by distance (IBD) were assessed in a viviparous coastal shark (the lemon shark Negaprion brevirostris) across 8 western Atlantic samples spaced between ∼150 and 7000 km apart. Juveniles (N = 325) were sequenced at 2 mitochondrial loci (1729 bp) and typed at 9 nuclear encoded microsatellite loci. Analysis of mitochondrial sequences revealed higher diversity at low-latitude island samples compared to highlatitude continental samples, consistent with an equatorial center-of-origin for this species. There were 5 distinct groups across our sampling areas (Brazil, Louisiana, Cape Canaveral, Gullivan Bay and the Florida Keys/Bahamas/Virgin Islands; pairwise ℙST = 0.07-0.87) and all but one pair of the 8 samples also exhibited significantly different haplotype frequencies (pairwise FST = 0.10-0.51). Bayesian analysis indicated that the Brazil and Louisiana samples were generally isolated from the others, but most of the rest were diverged although still connected or recently connected by migration. In contrast, structure was only detected between the most distant sample (Brazil) and all of the others using the microsatellite markers (pairwise FST = 0.03-0.06). There was a significant pattern of IBD for all markers and measures of genetic differentiation (r2 = 0.65-0.81, p < 0.05- 0.01), but not after removing the Brazil sample. There was evidence that glacial and post-glacial historical processes and sex-specific differences in philopatry affected IBD. Because of the relatively fine-scale population structure of this and other large coastal shark species more attention should be paid to local processes in the conservation and fisheries management of these species.
KW - Microsatellites
KW - Mitochondrial DNA
KW - Phylogeography
KW - Population structure
U2 - 10.3354/meps11069
DO - 10.3354/meps11069
JO - Marine Ecology - Progress Series
JF - Marine Ecology - Progress Series