The Analytical Biochemistry shared resource provides state-of-the-art chemistry services and expertise in mass spectrometry, chromatography, and analytical biochemistry. These three complementary specialties afford a center of expertise for a full range of chemical and biochemical services in support of the peer-reviewed research of Cancer Center investigators.
The Analytical Biochemistry shared resource provides advice and assistance in the development of state-of-the-art chromatographic separations. The focus is on methods using gas chromatography (GC) and high-performance liquid chromatography (HPLC). The chromatography specialist, Steven Carmella, will consult with Cancer Center investigators about method development or application of existing methods to analytical chemistry problems.
The Analytical Biochemistry shared resource has particular expertise in the chromatographic separation and quantitation of metabolites, adducts, and other compounds in biological matrices. Instruments in the facility are available for preliminary methods development studies. These instruments include Waters gradient solvent delivery systems with UV-Vis, fluorescence, and radioactivity detectors.
The chromatography specialist will also assist in solving instrumentation problems which Cancer Center investigators may encounter. The chromatography services function of the Analytical Biochemistry shared resource is a highly efficient and cost-effective solution to separation problems.
The Analytical Biochemistry shared resource provides a variety of molecular identification, characterization and quantitation capabilities to its users utilizing a variety of mass spectrometers (see below). Both liquid and gas chromatographic separations are provided as are a range of liquid flow rates and ion sources.
The facility provides analytical services including small molecule quantitation and identification, oligonucleotide characterization, and whole and digested protein sample analysis. A variety of quantitative assays of samples derived from complex in vitro and in vivo systems are routinely performed.
The standard operating model for the facility involves extensive training of users in both the use of instrumentation and data analysis using a variety of available software tools. Facility staff maintains and repairs instrumentation and provides guidance in method development and sample preparation as well assistance with troubleshooting of problems related to the users mass spectrometric analysis.
New users should contact facility staff (see below) for consultation regarding desired analytical services. Existing users can request spectrometry services by filling out a Mass Spectrometry Instrumentation Request online. Requests for usage of the Quantiva, Elite, and Fusion instruments will require consultation with Peter Villalta (see below for contact information).
We are located in a secure building. Users must fill out building access forms which can be found on the Research Building Management Services website.