Trophoblast Medium
TM consists of 500 ml of basal medium, 25 ml of fetal bovine serum (FBS, Cat. No. 0025), 5 ml of trophoblast growth supplement (TGS, Cat. No. 7152) and 5 ml of penicillin/streptomycin solution (P/S, Cat. No. 0503).
*Please select type of Medium below:
-- Please Select --Complete kit (includes supplements) - 7121 — $122.00Basal (no supplements) - 7121-b — $81.00Phenol Red Free (includes supplements) - 7121-prf — $126.00Basal Phenol Red Free (no supplements) -7121-b-prf — $85.00
Trophoblast Medium (TM), when used with Trophoblast Growth Supplement (TGS, Cat #7152) and 25 ml of fetal bovine serum (FBS) is a complete medium designed for optimal growth of normal trophoblasts in vitro. It is a sterile, liquid medium which contains essential and non-essential amino acids, vitamins, organic and inorganic compounds, hormones, growth factors, trace minerals and a low concentration of fetal bovine serum (5%). The medium is HEPES and bicarbonate buffered and has a pH of 7.4 when equilibrated in an incubator with an atmosphere of 5% CO2/95% air. The medium is formulated (quantitatively and qualitatively) to provide an optimally balanced nutritional environment that selectively supports the growth of normal trophoblasts in vitro.
Store the basal medium at 4&degC, the TGS, the FBS and the P/S solution at -20&degC. Protect from light.
Basal medium: room temperature. Supplements: dry ice.
7121-b (Basal Medium)
7121-b-prf (Basal & Phenol Red Free Medium)
7121-prf (Complete Phenol Red Free Medium)
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1.) Kudoh, A., Miyakawa, K., Matsunaga, S., Matsushima, Y., Kosugi, I., Kimura, H., Hayakawa, S., Sawasaki, T. & Ryo, A.(2016) 'H11/HSPB8 Restricts HIV2 Vpx to Restore the AntiViral Activity of SAMHD1' Front Microbiol. VOL 7
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0025 FBS Fetal Bovine Serum, 25 ml
0503 P/S Antibiotic Solution, 5 ml
7152 TGS Trophoblast Growth Supplement