Protein oxidation is frequently associated with excess oxidative stress in the cell. Oxidative stress is a cellular disorder caused by an excess of free radicals resulting from increased oxidant levels, decreased anti-oxidant amounts, or failure to repair damage induced by reactive oxygen species (ROS). Oxidative stress and oxidation have been linked to atherosclerosis, ischemia, cancer, rheumatoid arthritis, and neurodegeneration. ROS molecules can chemically modify proteins, lipids, and nucleic acids, potentially altering or impairing their normal functions.
YAP1 transcription factor is known to regulate the response to oxidative stress in S. cerevisiae. YAP1 is activated by increased levels of reactive oxygen species and moves quickly to the nucleus where it regulates the expression of up to 70 genes. Millipore's Anti-YAP1 antibody is routinely evaluated by Western Blot in human liver tissue lysate.
Millipore's anti-8 hydroxyguanosine has been shown by ELISA to be completely specific for oxidized DNA while not crossreacting with other naturally occurring nucleotides. This antibody is a valuable tool for elucidating the role of free radical damage in a number of human disease states.
MilliporeSigma's protein oxidation detection kits in WB (OxyBLOT™), Elisa (OxyELISA™), ICC (OxyICC), and IHC (OxyIHC) formats are extremely sensitivity in detecting carbonyl groups introduced on proteins as a result of oxidative stress, oxygen free radicals and other reactive species.
Oxidative stress is a cellular disorder caused by an excess of free radicals which can occur because of increased oxidant levels, decreased anti-oxidant amounts, or failure to repair oxidative damage induced by reactive oxygen species (ROS). This condition has been linked to the pathogenesis of a number of disease states including atherosclerosis, ischemia, cancer, rheumatoid arthritis, and neurodegeneration. We illustrate detection and quantification of carbonyl modifications using the OxyBLOT™ and OxyELISA™assays.