Spliceosomal introns in the deep-branching eukaryote Trichomonas vaginalis
Štěpánka ̌ Vaňáčová, Weihong Yan, Jane M. Carlton, Patricia J. Johnson
Biology and Genomics
Urban Initiative
Eukaryotes have evolved elaborate splicing mechanisms to remove introns that would otherwise destroy the protein-coding capacity of genes. Nuclear premRNA splicing requires sequence motifs in the intron and is mediated by a ribonucleoprotein complex, the spliceosome. Here we demonstrate the presence of a splicing apparatus in the protist Trichomonas vaginalis and show that RNA motifs found in yeast and metazoan introns are required for splicing. We also describe the first introns in this deep-branching lineage. The positions of these introns are often conserved in orthologous genes, indicating they were present in a common ancestor of trichomonads, yeast, and metazoa. All examined T. vaginalis introns have a highly conserved 12-nt 3′ splice-site motif that encompasses the branch point and is necessary for splicing. This motif is also found in the only described intron in a gene from another deep-branching eukaryote, Giardia intestinalis. These studies demonstrate the conservation of intron splicing signals across large evolutionary distances, reveal unexpected motif conservation in deep-branching lineages that suggest a simplified mechanism of splicing in primitive unicellular eukaryotes, and support the presence of introns in the earliest eukaryote.
Old introns
Fingerprint Dive into the research topics of 'Spliceosomal introns in the deep-branching eukaryote Trichomonas vaginalis'. Together they form a unique fingerprint.
Trichomonas vaginalis Medicine & Life Sciences
Eukaryota Medicine & Life Sciences
Introns Medicine & Life Sciences
Yeasts Medicine & Life Sciences
Spliceosomes Medicine & Life Sciences
Nucleotide Motifs Medicine & Life Sciences
Giardia lamblia Medicine & Life Sciences
RNA Splice Sites Medicine & Life Sciences
Vaňáčová, Š. ., Yan, W., Carlton, J. M., & Johnson, P. J. (2005). Spliceosomal introns in the deep-branching eukaryote Trichomonas vaginalis. Proceedings of the National Academy of Sciences of the United States of America, 102(12), 4430-4435. https://doi.org/10.1073/pnas.0407500102
Spliceosomal introns in the deep-branching eukaryote Trichomonas vaginalis. / Vaňáčová, Štěpánka ̌; Yan, Weihong; Carlton, Jane M.; Johnson, Patricia J.
In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 102, No. 12, 22.03.2005, p. 4430-4435.
Vaňáčová, Š, Yan, W, Carlton, JM & Johnson, PJ 2005, 'Spliceosomal introns in the deep-branching eukaryote Trichomonas vaginalis', Proceedings of the National Academy of Sciences of the United States of America, vol. 102, no. 12, pp. 4430-4435. https://doi.org/10.1073/pnas.0407500102
Vaňáčová Š, Yan W, Carlton JM, Johnson PJ. Spliceosomal introns in the deep-branching eukaryote Trichomonas vaginalis. Proceedings of the National Academy of Sciences of the United States of America. 2005 Mar 22;102(12):4430-4435. https://doi.org/10.1073/pnas.0407500102
Vaňáčová, Štěpánka ̌ ; Yan, Weihong ; Carlton, Jane M. ; Johnson, Patricia J. / Spliceosomal introns in the deep-branching eukaryote Trichomonas vaginalis. In: Proceedings of the National Academy of Sciences of the United States of America. 2005 ; Vol. 102, No. 12. pp. 4430-4435.
@article{690541ea7a5f466a90fa7292b4ee3881,
title = "Spliceosomal introns in the deep-branching eukaryote Trichomonas vaginalis",
abstract = "Eukaryotes have evolved elaborate splicing mechanisms to remove introns that would otherwise destroy the protein-coding capacity of genes. Nuclear premRNA splicing requires sequence motifs in the intron and is mediated by a ribonucleoprotein complex, the spliceosome. Here we demonstrate the presence of a splicing apparatus in the protist Trichomonas vaginalis and show that RNA motifs found in yeast and metazoan introns are required for splicing. We also describe the first introns in this deep-branching lineage. The positions of these introns are often conserved in orthologous genes, indicating they were present in a common ancestor of trichomonads, yeast, and metazoa. All examined T. vaginalis introns have a highly conserved 12-nt 3′ splice-site motif that encompasses the branch point and is necessary for splicing. This motif is also found in the only described intron in a gene from another deep-branching eukaryote, Giardia intestinalis. These studies demonstrate the conservation of intron splicing signals across large evolutionary distances, reveal unexpected motif conservation in deep-branching lineages that suggest a simplified mechanism of splicing in primitive unicellular eukaryotes, and support the presence of introns in the earliest eukaryote.",
keywords = "Evolution, Gene expression, Old introns, Splicing",
author = "Va{\v n}{\'a}{\v c}ov{\'a}, {{\v S}t{\v e}p{\'a}nka{\v }} and Weihong Yan and Carlton, {Jane M.} and Johnson, {Patricia J.}",
T1 - Spliceosomal introns in the deep-branching eukaryote Trichomonas vaginalis
AU - Vaňáčová, Štěpánka ̌
AU - Yan, Weihong
AU - Carlton, Jane M.
AU - Johnson, Patricia J.
N2 - Eukaryotes have evolved elaborate splicing mechanisms to remove introns that would otherwise destroy the protein-coding capacity of genes. Nuclear premRNA splicing requires sequence motifs in the intron and is mediated by a ribonucleoprotein complex, the spliceosome. Here we demonstrate the presence of a splicing apparatus in the protist Trichomonas vaginalis and show that RNA motifs found in yeast and metazoan introns are required for splicing. We also describe the first introns in this deep-branching lineage. The positions of these introns are often conserved in orthologous genes, indicating they were present in a common ancestor of trichomonads, yeast, and metazoa. All examined T. vaginalis introns have a highly conserved 12-nt 3′ splice-site motif that encompasses the branch point and is necessary for splicing. This motif is also found in the only described intron in a gene from another deep-branching eukaryote, Giardia intestinalis. These studies demonstrate the conservation of intron splicing signals across large evolutionary distances, reveal unexpected motif conservation in deep-branching lineages that suggest a simplified mechanism of splicing in primitive unicellular eukaryotes, and support the presence of introns in the earliest eukaryote.
AB - Eukaryotes have evolved elaborate splicing mechanisms to remove introns that would otherwise destroy the protein-coding capacity of genes. Nuclear premRNA splicing requires sequence motifs in the intron and is mediated by a ribonucleoprotein complex, the spliceosome. Here we demonstrate the presence of a splicing apparatus in the protist Trichomonas vaginalis and show that RNA motifs found in yeast and metazoan introns are required for splicing. We also describe the first introns in this deep-branching lineage. The positions of these introns are often conserved in orthologous genes, indicating they were present in a common ancestor of trichomonads, yeast, and metazoa. All examined T. vaginalis introns have a highly conserved 12-nt 3′ splice-site motif that encompasses the branch point and is necessary for splicing. This motif is also found in the only described intron in a gene from another deep-branching eukaryote, Giardia intestinalis. These studies demonstrate the conservation of intron splicing signals across large evolutionary distances, reveal unexpected motif conservation in deep-branching lineages that suggest a simplified mechanism of splicing in primitive unicellular eukaryotes, and support the presence of introns in the earliest eukaryote.
KW - Evolution
KW - Old introns
KW - Splicing