Interleukin-32 induces the differentiation of monocytes into macrophage-like cells
Mihai G. Netea, Eli C. Lewis, Tania Azam, Leo A.B. Joosten, Jun Jaekal, Su Young Bae, Charles A. Dinarello, Soo Hyun Kim
After emigration from the bone marrow to the peripheral blood, monocytes enter tissues and differentiate into macrophages, the prototype scavenger of the immune system. By ingesting and killing microorganisms and removing cellular debris, macrophages also process antigens as a first step in mounting a specific immune response. IL-32 is a cytokine inducing proinflammatory cytokines and chemokines via p38-MAPK and NF-κB. In the present study, we demonstrate that IL-32 induces differentiation of human blood monocytes as well as THP-1 leukemic cells into macrophage-like cells with functional phagocytic activity for live bacteria. Muramyl dipepide (MDP), the ligand for the intracellular nuclear oligomerization domain (NOD) 2 receptor, has no effect on differentiation alone but augments the monocyte-to-macrophage differentiation by IL-32. Unexpectedly, IL-32 reversed GM-CSF/IL-4-induced dendritic cell differentiation to macrophage-like cells. Whereas the induction of TNFα, IL-1β, and IL-6 by IL-32 is mediated by p38-MAPK, IL-32-induced monocyte-to-macrophage differentiation is mediated through nonapoptotic, caspase-3-dependent mechanisms. Thus, IL-32 not only contributes to host responses through the induction of proinflammatory cytokines but also directly affects specific immunity by differentiating monocytes into macrophage-like cells.
Proceedings of the National Academy of Sciences of the United States of America
https://doi.org/10.1073/pnas.0712381105
Published - 4 Mar 2008
Antigen presentation
Cytokine
Immune cell differentiation
Muramyl dipeptide
10.1073/pnas.0712381105
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Interleukins Medicine & Life Sciences 100%
Monocytes Medicine & Life Sciences 75%
p38 Mitogen-Activated Protein Kinases Medicine & Life Sciences 26%
Cytokines Medicine & Life Sciences 24%
THP-1 Cells Medicine & Life Sciences 15%
Emigration and Immigration Medicine & Life Sciences 15%
Granulocyte-Macrophage Colony-Stimulating Factor Medicine & Life Sciences 13%
Netea, M. G., Lewis, E. C., Azam, T., Joosten, L. A. B., Jaekal, J., Bae, S. Y., Dinarello, C. A., & Kim, S. H. (2008). Interleukin-32 induces the differentiation of monocytes into macrophage-like cells. Proceedings of the National Academy of Sciences of the United States of America, 105(9), 3515-3520. https://doi.org/10.1073/pnas.0712381105
Netea, Mihai G. ; Lewis, Eli C. ; Azam, Tania ; Joosten, Leo A.B. ; Jaekal, Jun ; Bae, Su Young ; Dinarello, Charles A. ; Kim, Soo Hyun. / Interleukin-32 induces the differentiation of monocytes into macrophage-like cells. In: Proceedings of the National Academy of Sciences of the United States of America. 2008 ; Vol. 105, No. 9. pp. 3515-3520.
@article{6cabdc9ca6f04c4890986065857ed429,
title = "Interleukin-32 induces the differentiation of monocytes into macrophage-like cells",
abstract = "After emigration from the bone marrow to the peripheral blood, monocytes enter tissues and differentiate into macrophages, the prototype scavenger of the immune system. By ingesting and killing microorganisms and removing cellular debris, macrophages also process antigens as a first step in mounting a specific immune response. IL-32 is a cytokine inducing proinflammatory cytokines and chemokines via p38-MAPK and NF-κB. In the present study, we demonstrate that IL-32 induces differentiation of human blood monocytes as well as THP-1 leukemic cells into macrophage-like cells with functional phagocytic activity for live bacteria. Muramyl dipepide (MDP), the ligand for the intracellular nuclear oligomerization domain (NOD) 2 receptor, has no effect on differentiation alone but augments the monocyte-to-macrophage differentiation by IL-32. Unexpectedly, IL-32 reversed GM-CSF/IL-4-induced dendritic cell differentiation to macrophage-like cells. Whereas the induction of TNFα, IL-1β, and IL-6 by IL-32 is mediated by p38-MAPK, IL-32-induced monocyte-to-macrophage differentiation is mediated through nonapoptotic, caspase-3-dependent mechanisms. Thus, IL-32 not only contributes to host responses through the induction of proinflammatory cytokines but also directly affects specific immunity by differentiating monocytes into macrophage-like cells.",
keywords = "Antigen presentation, Cytokine, Immune cell differentiation, Inflammation, Muramyl dipeptide",
author = "Netea, {Mihai G.} and Lewis, {Eli C.} and Tania Azam and Joosten, {Leo A.B.} and Jun Jaekal and Bae, {Su Young} and Dinarello, {Charles A.} and Kim, {Soo Hyun}",
doi = "10.1073/pnas.0712381105",
journal = "Proceedings of the National Academy of Sciences of the United States of America",
publisher = "National Academy of Sciences",
Netea, MG, Lewis, EC, Azam, T, Joosten, LAB, Jaekal, J, Bae, SY, Dinarello, CA & Kim, SH 2008, 'Interleukin-32 induces the differentiation of monocytes into macrophage-like cells', Proceedings of the National Academy of Sciences of the United States of America, vol. 105, no. 9, pp. 3515-3520. https://doi.org/10.1073/pnas.0712381105
Interleukin-32 induces the differentiation of monocytes into macrophage-like cells. / Netea, Mihai G.; Lewis, Eli C.; Azam, Tania; Joosten, Leo A.B.; Jaekal, Jun; Bae, Su Young; Dinarello, Charles A.; Kim, Soo Hyun.
In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 105, No. 9, 04.03.2008, p. 3515-3520.
T1 - Interleukin-32 induces the differentiation of monocytes into macrophage-like cells
AU - Netea, Mihai G.
AU - Lewis, Eli C.
AU - Azam, Tania
AU - Joosten, Leo A.B.
AU - Jaekal, Jun
AU - Bae, Su Young
AU - Dinarello, Charles A.
AU - Kim, Soo Hyun
N2 - After emigration from the bone marrow to the peripheral blood, monocytes enter tissues and differentiate into macrophages, the prototype scavenger of the immune system. By ingesting and killing microorganisms and removing cellular debris, macrophages also process antigens as a first step in mounting a specific immune response. IL-32 is a cytokine inducing proinflammatory cytokines and chemokines via p38-MAPK and NF-κB. In the present study, we demonstrate that IL-32 induces differentiation of human blood monocytes as well as THP-1 leukemic cells into macrophage-like cells with functional phagocytic activity for live bacteria. Muramyl dipepide (MDP), the ligand for the intracellular nuclear oligomerization domain (NOD) 2 receptor, has no effect on differentiation alone but augments the monocyte-to-macrophage differentiation by IL-32. Unexpectedly, IL-32 reversed GM-CSF/IL-4-induced dendritic cell differentiation to macrophage-like cells. Whereas the induction of TNFα, IL-1β, and IL-6 by IL-32 is mediated by p38-MAPK, IL-32-induced monocyte-to-macrophage differentiation is mediated through nonapoptotic, caspase-3-dependent mechanisms. Thus, IL-32 not only contributes to host responses through the induction of proinflammatory cytokines but also directly affects specific immunity by differentiating monocytes into macrophage-like cells.
AB - After emigration from the bone marrow to the peripheral blood, monocytes enter tissues and differentiate into macrophages, the prototype scavenger of the immune system. By ingesting and killing microorganisms and removing cellular debris, macrophages also process antigens as a first step in mounting a specific immune response. IL-32 is a cytokine inducing proinflammatory cytokines and chemokines via p38-MAPK and NF-κB. In the present study, we demonstrate that IL-32 induces differentiation of human blood monocytes as well as THP-1 leukemic cells into macrophage-like cells with functional phagocytic activity for live bacteria. Muramyl dipepide (MDP), the ligand for the intracellular nuclear oligomerization domain (NOD) 2 receptor, has no effect on differentiation alone but augments the monocyte-to-macrophage differentiation by IL-32. Unexpectedly, IL-32 reversed GM-CSF/IL-4-induced dendritic cell differentiation to macrophage-like cells. Whereas the induction of TNFα, IL-1β, and IL-6 by IL-32 is mediated by p38-MAPK, IL-32-induced monocyte-to-macrophage differentiation is mediated through nonapoptotic, caspase-3-dependent mechanisms. Thus, IL-32 not only contributes to host responses through the induction of proinflammatory cytokines but also directly affects specific immunity by differentiating monocytes into macrophage-like cells.
KW - Antigen presentation
KW - Cytokine
KW - Immune cell differentiation
KW - Inflammation
KW - Muramyl dipeptide
U2 - 10.1073/pnas.0712381105
DO - 10.1073/pnas.0712381105
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
Netea MG, Lewis EC, Azam T, Joosten LAB, Jaekal J, Bae SY et al. Interleukin-32 induces the differentiation of monocytes into macrophage-like cells. Proceedings of the National Academy of Sciences of the United States of America. 2008 Mar 4;105(9):3515-3520. https://doi.org/10.1073/pnas.0712381105