The enzyme often forms a two-component system with monooxygenases. Unlike EC 1.5.1.38, FMN reductase (NADPH), and EC 1.5.1.39, FMN reductase [NAD(P)H], this enzyme has a strong preference for NADH over NADPH, although some activity with the latter is observed [1,2]. While FMN is the preferred substrate, FAD can also be used with much lower activity [1,3].
Izumoto, Y.; Mori, T.; Yamamoto, K.
Kamali, N.; Tavallaie, M.; Bambai, B.; Karkhane, A.A.; Miri, M.
Bezrukikh, A.; Esimbekova, E.; Nemtseva, E.; Kratasyuk, V.; Shimomura, O.
Tinikul, R.; Pitsawong, W.; Sucharitakul, J.; Nijvipakul, S.; Ballou, D.P.; Chaiyen, P.
Sousa, S.F.; Sousa, J.F.; Barbosa, A.C.; Ferreira, C.E.; Neves, R.P.; Ribeiro, A.J.; Fernandes, P.A.; Ramos, M.J.