Introduction: Metabolic dyslipidemia and elevated oxidative stress are very common in patients with diabetes and metabolic syndrome. The aim of this study was to investigate the effects of melatonin, on the plasma lipid profile and levels of MDA in tissues of fructose fed rats. Materials and Methods: Twenty-four male Wistar rats were divided into three groups: 1. controls that received normal chow and tap water. 2. fructose group that received chow +10% fructose solution in drinking water. 3. The melatonin group that received chow +10% fructose solution+ daily injection of 10 mg/kg (BW) melatonin (ip). After 8 weeks, plasma concentrations of triglycerides (TG), Total cholesterol (TC), low density lipoprotein(LDL), high density lipoprotein (HDL), and MDA in the tissues were measured and the Atherogenic index(AI) was calculated. Results: The fructose fed rats showed significantly higher levels of TG, (p=0.01) compared to control rats, not in the melatonin group. HDL concentrations showed significant decrease in fructose rats, but not in the melatonin group. TC and LDL did not change significantly.AI increase in fructose rats(p=0.00) and decrease in melatonin treated rats(p=0.01). The fructose fed rats had higher MDA values compared with controls and melatonin administration decreased MDA values in heart, kidney and liver tissue. Conclusion: Melatonin intake can regulate metabolic dyslipidemia and decrease MDA levels in fructose fed rats.