Texas Red conjugated secondary antibody.
11. Secondary antibody: incubate sections with FITC conjugated secondary antibody in PBS for 30 minutes at room temperature.
13. 2nd primary antibody: incubate sections with 2nd primary antibody diluted with PBS for 1 hour or overnight at 4 °C.
14. Rinse 3x5 min in PBS.
15. Secondary antibody: incubate sections with Texas Red conjugated secondary antibody in PBS for 30 minutes at room temperature.
17. Rinse 3x5min in PBS.
18. Coverslip with aqueous mounting medium and seal with nail polish.
19. Observation using a fluorescence microscope with appropriate filters. Store slides in the dark at 4 °C.