Deoxyribonucleic Acid Reassociation in the Classification of Flavobacteria
R. J. Owen1, J. J. S. Snell1
Affiliations: 1 National Collection of Type Cultures, Central Public Health Laboratory, London NW9 5HT
First Published: 01 March 1976 https://doi.org/10.1099/00221287-93-1-89
Summary: DNA-DNA reassociation studies showed that Flavobacterium meningosepticum strains had a genetic relatedness of 91 to 100% with inter-strain duplexes having high thermal stabilities. The only exception, strain NCTC10016, had an average relatedness of only 43% to other strains of F. meningosepticum. The apparent divergence in DNA base sequence of this strain was reflected in the structural differences of some enzymes. There was a gradation of DNA relatedness among the Flavobacterium group II-b strains, but three strains were sufficiently related to constitute a species. Low levels of genetic relatedness were confirmed between F. meningosepticum and strains of Flavobacterium group II-b, group II-f, F. aquatile, F. breve, F. heparinum, F. pectinovorum, F. odoratum and Moraxella saccharolytica. All strains had base compositions in the range 32 to 46% guanine plus cytosine. The genome sizes of representative strains of F. meningosepticum and Flavobacterium group II-b were 2·50 × 109 to 3·52 × 109 daltons, whereas the group II-f strains had smaller genomes of 1·64 × 109 to 1·81 × 109 daltons. The taxonomic implications of these findings are discussed.
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Microbiology 93, 89 (1976); https://doi.org/10.1099/00221287-93-1-89