In both prokaryotic and eukaryotic cells, multiple ribosomes may be attached to a single mRNA, generating a structure called a polyribosome.
We have now considered the process of translation in bacterial cells and noted some distinctive differences that exist in eukaryotic cells. Let's take a few minutes to reflect on some of the important similarities and differences of protein synthesis in bacterial and eukaryotic cells.
The polypeptide is released from the tRNA in the P site.
When the ribosome translocates to a stop codon, there is no tRNA with an anticodon that can pair with the codon in the A site.
^ .and RF3 forms a complex with GTP and binds to the ribosome.
I GTP associated with RF3 is hydrolyzed to GDP.
release factors are released from the ribosome.
Conclusion: When a stop codon is encountered, release factors associate with the ribosome and bring about the termination of translation.