Sequence polymorphisms of Der f 1, Der p 1, Der f 2 and Der p 2 from Korean house dust mite isolates
Kyoung Yong Jeong, In Yong Lee, Tai Soon Yong, Jae Hyun Lee, Eun Jin Kim, Joo Shil Lee, Chein Soo Hong, Jung Won Park
Department of Environmental Medical Biology
Amino acid sequence variations have possible influences on the allergenicity of allergens and may be important factors in allergen standardization. This study was undertaken to investigate the sequence polymorphisms of group 1 and 2 allergens from Korean isolates of the house dust mites Dermatophagoides farinae and D. pteronyssinus. cDNA sequences encoding group 1 and 2 allergens were amplified by RT-PCR and compared the deduced amino acid sequences. Der f 1. 0101, which appeared in 64. 0 % of the 50 sequences analyzed, was found to be predominant. Among the Der p 1 sequences, Der p 1. 0102 and 1. 0105 were predominant (58 %). Among the Der f 2 sequences, Der f 2. 0102 (40. 7 %) and a new variant with Gly at position 42 (27. 8 %) were predominant. The deduced amino acid sequences of 60 Der p 2 clones were examined, and 28 variants with 1-5 amino acid substitutions were found. Interestingly, all of the Der p 2 sequences had Thr instead of Lys at position 49. Two variants (Leu40, Thr49, and Asn114 (26. 6 %); Val40, Thr49, and Asn114 (20. 0 %)) were found to be the most predominant forms of Der p 2. Der p 1 has a high rate of sporadic substitutions and the group 2 allergens show a more regular pattern with orderly associations of amino acid substitutions. Der f 1 and Der p 2 from Korean mite isolates have unique amino acid sequence polymorphisms. These findings provide important data for house dust mite allergen standardization.
Experimental and Applied Acarology
genetic polymorphism
amino acid sequences
Dermatophagoides pteronyssinus
Dermatophagoides farinae
allergenicity
allergen
Jeong, K. Y., Lee, I. Y., Yong, T. S., Lee, J. H., Kim, E. J., Lee, J. S., ... Park, J. W. (2012). Sequence polymorphisms of Der f 1, Der p 1, Der f 2 and Der p 2 from Korean house dust mite isolates. Experimental and Applied Acarology, 58(1), 35-42. https://doi.org/10.1007/s10493-012-9553-x
Jeong, Kyoung Yong ; Lee, In Yong ; Yong, Tai Soon ; Lee, Jae Hyun ; Kim, Eun Jin ; Lee, Joo Shil ; Hong, Chein Soo ; Park, Jung Won. / Sequence polymorphisms of Der f 1, Der p 1, Der f 2 and Der p 2 from Korean house dust mite isolates. In: Experimental and Applied Acarology. 2012 ; Vol. 58, No. 1. pp. 35-42.
@article{ca8f8b6e745c47bfabc78356d4777259,
title = "Sequence polymorphisms of Der f 1, Der p 1, Der f 2 and Der p 2 from Korean house dust mite isolates",
abstract = "Amino acid sequence variations have possible influences on the allergenicity of allergens and may be important factors in allergen standardization. This study was undertaken to investigate the sequence polymorphisms of group 1 and 2 allergens from Korean isolates of the house dust mites Dermatophagoides farinae and D. pteronyssinus. cDNA sequences encoding group 1 and 2 allergens were amplified by RT-PCR and compared the deduced amino acid sequences. Der f 1. 0101, which appeared in 64. 0 {\%} of the 50 sequences analyzed, was found to be predominant. Among the Der p 1 sequences, Der p 1. 0102 and 1. 0105 were predominant (58 {\%}). Among the Der f 2 sequences, Der f 2. 0102 (40. 7 {\%}) and a new variant with Gly at position 42 (27. 8 {\%}) were predominant. The deduced amino acid sequences of 60 Der p 2 clones were examined, and 28 variants with 1-5 amino acid substitutions were found. Interestingly, all of the Der p 2 sequences had Thr instead of Lys at position 49. Two variants (Leu40, Thr49, and Asn114 (26. 6 {\%}); Val40, Thr49, and Asn114 (20. 0 {\%})) were found to be the most predominant forms of Der p 2. Der p 1 has a high rate of sporadic substitutions and the group 2 allergens show a more regular pattern with orderly associations of amino acid substitutions. Der f 1 and Der p 2 from Korean mite isolates have unique amino acid sequence polymorphisms. These findings provide important data for house dust mite allergen standardization.",
author = "Jeong, {Kyoung Yong} and Lee, {In Yong} and Yong, {Tai Soon} and Lee, {Jae Hyun} and Kim, {Eun Jin} and Lee, {Joo Shil} and Hong, {Chein Soo} and Park, {Jung Won}",
journal = "Experimental and Applied Acarology",
Jeong, KY, Lee, IY, Yong, TS, Lee, JH, Kim, EJ, Lee, JS, Hong, CS & Park, JW 2012, 'Sequence polymorphisms of Der f 1, Der p 1, Der f 2 and Der p 2 from Korean house dust mite isolates', Experimental and Applied Acarology, vol. 58, no. 1, pp. 35-42. https://doi.org/10.1007/s10493-012-9553-x
Sequence polymorphisms of Der f 1, Der p 1, Der f 2 and Der p 2 from Korean house dust mite isolates. / Jeong, Kyoung Yong; Lee, In Yong; Yong, Tai Soon; Lee, Jae Hyun; Kim, Eun Jin; Lee, Joo Shil; Hong, Chein Soo; Park, Jung Won.
In: Experimental and Applied Acarology, Vol. 58, No. 1, 01.09.2012, p. 35-42.
T1 - Sequence polymorphisms of Der f 1, Der p 1, Der f 2 and Der p 2 from Korean house dust mite isolates
AU - Jeong, Kyoung Yong
AU - Lee, In Yong
AU - Yong, Tai Soon
AU - Lee, Jae Hyun
AU - Kim, Eun Jin
AU - Lee, Joo Shil
AU - Hong, Chein Soo
AU - Park, Jung Won
N2 - Amino acid sequence variations have possible influences on the allergenicity of allergens and may be important factors in allergen standardization. This study was undertaken to investigate the sequence polymorphisms of group 1 and 2 allergens from Korean isolates of the house dust mites Dermatophagoides farinae and D. pteronyssinus. cDNA sequences encoding group 1 and 2 allergens were amplified by RT-PCR and compared the deduced amino acid sequences. Der f 1. 0101, which appeared in 64. 0 % of the 50 sequences analyzed, was found to be predominant. Among the Der p 1 sequences, Der p 1. 0102 and 1. 0105 were predominant (58 %). Among the Der f 2 sequences, Der f 2. 0102 (40. 7 %) and a new variant with Gly at position 42 (27. 8 %) were predominant. The deduced amino acid sequences of 60 Der p 2 clones were examined, and 28 variants with 1-5 amino acid substitutions were found. Interestingly, all of the Der p 2 sequences had Thr instead of Lys at position 49. Two variants (Leu40, Thr49, and Asn114 (26. 6 %); Val40, Thr49, and Asn114 (20. 0 %)) were found to be the most predominant forms of Der p 2. Der p 1 has a high rate of sporadic substitutions and the group 2 allergens show a more regular pattern with orderly associations of amino acid substitutions. Der f 1 and Der p 2 from Korean mite isolates have unique amino acid sequence polymorphisms. These findings provide important data for house dust mite allergen standardization.
AB - Amino acid sequence variations have possible influences on the allergenicity of allergens and may be important factors in allergen standardization. This study was undertaken to investigate the sequence polymorphisms of group 1 and 2 allergens from Korean isolates of the house dust mites Dermatophagoides farinae and D. pteronyssinus. cDNA sequences encoding group 1 and 2 allergens were amplified by RT-PCR and compared the deduced amino acid sequences. Der f 1. 0101, which appeared in 64. 0 % of the 50 sequences analyzed, was found to be predominant. Among the Der p 1 sequences, Der p 1. 0102 and 1. 0105 were predominant (58 %). Among the Der f 2 sequences, Der f 2. 0102 (40. 7 %) and a new variant with Gly at position 42 (27. 8 %) were predominant. The deduced amino acid sequences of 60 Der p 2 clones were examined, and 28 variants with 1-5 amino acid substitutions were found. Interestingly, all of the Der p 2 sequences had Thr instead of Lys at position 49. Two variants (Leu40, Thr49, and Asn114 (26. 6 %); Val40, Thr49, and Asn114 (20. 0 %)) were found to be the most predominant forms of Der p 2. Der p 1 has a high rate of sporadic substitutions and the group 2 allergens show a more regular pattern with orderly associations of amino acid substitutions. Der f 1 and Der p 2 from Korean mite isolates have unique amino acid sequence polymorphisms. These findings provide important data for house dust mite allergen standardization.
JO - Experimental and Applied Acarology
JF - Experimental and Applied Acarology
Jeong KY, Lee IY, Yong TS, Lee JH, Kim EJ, Lee JS et al. Sequence polymorphisms of Der f 1, Der p 1, Der f 2 and Der p 2 from Korean house dust mite isolates. Experimental and Applied Acarology. 2012 Sep 1;58(1):35-42. https://doi.org/10.1007/s10493-012-9553-x