Antibody was affinity purified using an epitope specific to MCM4 immobilized on solid support. The epitope recognized by A300-193A maps to a region between residues 750 and 800 of human Minichromosome Maintenance 4 using the numbering given in entry NP_005905.2 (GeneID 4173).Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG..
Detection of human MCM4 by western blot. Sample: RIPA extract (50 µg) from HeLa cells. Antibody: Affinity purified rabbit anti-MCM4 (Cat. No. A300-193A) used at 0.2 µg/ml. Detection: Chemiluminescence.