search filter All ContentAll JournalsEmerging Topics in Life Sciences
Ahead-of-Issue Articles
Royal Society of Biology
Issue Editors
Mattheos A.G. Koffas;
Mattheos A.G. Koffas
Robert J. Linhardt
The lack of availability of glycans and glycoconjugates has severely limited the development of the field of glycobiology. This issue of Emerging Topics in Life Sciences features nine reviews by respected scientists in the field and deals with the application of metabolic engineering to the production of these complex carbohydrate or saccharide structures. The cover image illustrates that capsular polysaccharides and other bioengineered compounds can be generated by engineered cells, such as Escherichia coli, through the application of various metabolic engineering tools and strategies. The image was kindly provided by Robert J. Linhardt (Rensselaer Polytechnic Institute) and designed by Asher Williams (Rensselaer Polytechnic Institute).
Review Article| August 06 2018
Metabolic engineering of glycosylated polyketide biosynthesis
Ramesh Prasad Pandey
Department of BT-Convergent Pharmaceutical Engineering, Sun Moon University, 70 Sunmoon-ro 221, Tangjeong-myeon, Asan-si, Chungnam 31460, Republic of Korea
Department of Life Science and Biochemical Engineering, Sun Moon University, 70 Sunmoon-ro 221, Tangjeong-myeon, Asan-si, Chungnam 31460, Republic of Korea
Prakash Parajuli
Jae Kyung Sohng
Correspondence: Jae Kyung Sohng (sohng@sunmoon.ac.kr)
Emerg Top Life Sci (2018) 2 (3): 389–403.
https://doi.org/10.1042/ETLS20180011
Revision Received:
Mattheos A.G. Koffas, Robert J. Linhardt, Ramesh Prasad Pandey, Prakash Parajuli, Jae Kyung Sohng; Metabolic engineering of glycosylated polyketide biosynthesis. Emerg Top Life Sci 26 October 2018; 2 (3): 389–403. doi: https://doi.org/10.1042/ETLS20180011
Microbial cell factories are extensively used for the biosynthesis of value-added chemicals, biopharmaceuticals, and biofuels. Microbial biosynthesis is also realistic for the production of heterologous molecules including complex natural products of plant and microbial origin. Glycosylation is a well-known post-modification method to engineer sugar-functionalized natural products. It is of particular interest to chemical biologists to increase chemical diversity of molecules. Employing the state-of-the-art systems and synthetic biology tools, a range of small to complex glycosylated natural products have been produced from microbes using a simple and sustainable fermentation approach. In this context, this review covers recent notable metabolic engineering approaches used for the biosynthesis of glycosylated plant and microbial polyketides in different microorganisms. This review article is broadly divided into two major parts. The first part is focused on the biosynthesis of glycosylated plant polyketides in prokaryotes and yeast cells, while the second part is focused on the generation of glycosylated microbial polyketides in actinomycetes.
glycosylation, metabolic engineering, natural products, polyketides
Biotechnology, Metabolism, Microbiology, Synthetic Biology
© 2018 The Author(s). Published by Portland Press Limited on behalf of the Biochemical Society and the Royal Society of Biology
Ahead-of-Issue article Alert
Parasite defense mechanisms in bees: behavior, immunity, antimicrobials, and symbionts
From bacterial battles to CRISPR crops; progress towards agricultural applications of genome editing
Confronting the grey zone after severe brain injury
On visions and promises — ethical aspects of in vitro meat
Mode of inhibition of HIV-1 reverse transcriptase by polyacetylenetriol, a novel inhibitor of RNA- and DNA-directed DNA polymerases
Biochem J (March, 2002)
Enzyme alchemy: cell-free synthetic biochemistry for natural products
Emerg Top Life Sci (September, 2019)
Escherichia coli engineered to synthesize isopentenyl diphosphate and dimethylallyl diphosphate from mevalonate: a novel system for the genetic analysis of the 2-C-methyl-d-erythritol 4-phosphate pathway for isoprenoid biosynthesis
Biochem J (December, 2000)
Manipulation of the ATP pool as a tool for metabolic engineering
Biochem Soc Trans (November, 2015)