Lipid detection, identification, and imaging single cells with SIMS
Michael L. Heien, Paul D. Piehowski, Nicholas Winograd, Andrew G. Ewing
Department of Neural and Behavioral Sciences
Time-of-flight secondary ion mass spectrometry (ToF-SIMS) can be utilized to map the distribution of various molecules on a surface with submicrometer resolution. Many of its biological applications have been in the study of membrane lipids, such as phospholipids and cholesterol. For these studies, the effectiveness of chemical mapping is limited by low signal intensity from various biomolecules. Because of the high-energy nature of the SIMS ionization process, many molecules are identified by detection of characteristic fragments. Cluster ion sources are able to increase ionization, leading to increased information collected from a surface. In this chapter, we highlight the utility of SIMS to image lipids at single cells. Particularly, we will describe sample preparation, data collection, and the analysis of lipids for two systems; rat oligodendrocytes and Tetrahymena thermophila. SIMS spectra yield information regarding lipid identity and concentration across cell surface.
Mass Spectrometry Imaging
Principles and Protocols
Stanislav Rubakhin, Jonathan Sweedler
https://doi.org/10.1007/978-1-60761-746-4_4
Methods in Molecular Biology
10.1007/978-1-60761-746-4_4
Dive into the research topics of 'Lipid detection, identification, and imaging single cells with SIMS'. Together they form a unique fingerprint.
Lipids Medicine & Life Sciences 100%
Secondary Ion Mass Spectrometry Medicine & Life Sciences 85%
Tetrahymena thermophila Medicine & Life Sciences 79%
Membrane Lipids Medicine & Life Sciences 56%
Oligodendroglia Medicine & Life Sciences 56%
Phospholipids Medicine & Life Sciences 45%
Ions Medicine & Life Sciences 42%
Data Analysis Medicine & Life Sciences 38%
Heien, M. L., Piehowski, P. D., Winograd, N., & Ewing, A. G. (2010). Lipid detection, identification, and imaging single cells with SIMS. In S. Rubakhin, & J. Sweedler (Eds.), Mass Spectrometry Imaging: Principles and Protocols (pp. 85-97). (Methods in Molecular Biology; Vol. 656). https://doi.org/10.1007/978-1-60761-746-4_4
Heien, Michael L. ; Piehowski, Paul D. ; Winograd, Nicholas et al. / Lipid detection, identification, and imaging single cells with SIMS. Mass Spectrometry Imaging: Principles and Protocols. editor / Stanislav Rubakhin ; Jonathan Sweedler. 2010. pp. 85-97 (Methods in Molecular Biology).
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title = "Lipid detection, identification, and imaging single cells with SIMS",
abstract = "Time-of-flight secondary ion mass spectrometry (ToF-SIMS) can be utilized to map the distribution of various molecules on a surface with submicrometer resolution. Many of its biological applications have been in the study of membrane lipids, such as phospholipids and cholesterol. For these studies, the effectiveness of chemical mapping is limited by low signal intensity from various biomolecules. Because of the high-energy nature of the SIMS ionization process, many molecules are identified by detection of characteristic fragments. Cluster ion sources are able to increase ionization, leading to increased information collected from a surface. In this chapter, we highlight the utility of SIMS to image lipids at single cells. Particularly, we will describe sample preparation, data collection, and the analysis of lipids for two systems; rat oligodendrocytes and Tetrahymena thermophila. SIMS spectra yield information regarding lipid identity and concentration across cell surface.",
author = "Heien, {Michael L.} and Piehowski, {Paul D.} and Nicholas Winograd and Ewing, {Andrew G.}",
doi = "10.1007/978-1-60761-746-4_4",
series = "Methods in Molecular Biology",
editor = "Stanislav Rubakhin and Jonathan Sweedler",
booktitle = "Mass Spectrometry Imaging",
Heien, ML, Piehowski, PD, Winograd, N & Ewing, AG 2010, Lipid detection, identification, and imaging single cells with SIMS. in S Rubakhin & J Sweedler (eds), Mass Spectrometry Imaging: Principles and Protocols. Methods in Molecular Biology, vol. 656, pp. 85-97. https://doi.org/10.1007/978-1-60761-746-4_4
Lipid detection, identification, and imaging single cells with SIMS. / Heien, Michael L.; Piehowski, Paul D.; Winograd, Nicholas et al.
Mass Spectrometry Imaging: Principles and Protocols. ed. / Stanislav Rubakhin; Jonathan Sweedler. 2010. p. 85-97 (Methods in Molecular Biology; Vol. 656).
T1 - Lipid detection, identification, and imaging single cells with SIMS
AU - Heien, Michael L.
AU - Piehowski, Paul D.
AU - Winograd, Nicholas
AU - Ewing, Andrew G.
N2 - Time-of-flight secondary ion mass spectrometry (ToF-SIMS) can be utilized to map the distribution of various molecules on a surface with submicrometer resolution. Many of its biological applications have been in the study of membrane lipids, such as phospholipids and cholesterol. For these studies, the effectiveness of chemical mapping is limited by low signal intensity from various biomolecules. Because of the high-energy nature of the SIMS ionization process, many molecules are identified by detection of characteristic fragments. Cluster ion sources are able to increase ionization, leading to increased information collected from a surface. In this chapter, we highlight the utility of SIMS to image lipids at single cells. Particularly, we will describe sample preparation, data collection, and the analysis of lipids for two systems; rat oligodendrocytes and Tetrahymena thermophila. SIMS spectra yield information regarding lipid identity and concentration across cell surface.
AB - Time-of-flight secondary ion mass spectrometry (ToF-SIMS) can be utilized to map the distribution of various molecules on a surface with submicrometer resolution. Many of its biological applications have been in the study of membrane lipids, such as phospholipids and cholesterol. For these studies, the effectiveness of chemical mapping is limited by low signal intensity from various biomolecules. Because of the high-energy nature of the SIMS ionization process, many molecules are identified by detection of characteristic fragments. Cluster ion sources are able to increase ionization, leading to increased information collected from a surface. In this chapter, we highlight the utility of SIMS to image lipids at single cells. Particularly, we will describe sample preparation, data collection, and the analysis of lipids for two systems; rat oligodendrocytes and Tetrahymena thermophila. SIMS spectra yield information regarding lipid identity and concentration across cell surface.
U2 - 10.1007/978-1-60761-746-4_4
DO - 10.1007/978-1-60761-746-4_4
T3 - Methods in Molecular Biology
BT - Mass Spectrometry Imaging
A2 - Rubakhin, Stanislav
A2 - Sweedler, Jonathan
Heien ML, Piehowski PD, Winograd N, Ewing AG. Lipid detection, identification, and imaging single cells with SIMS. In Rubakhin S, Sweedler J, editors, Mass Spectrometry Imaging: Principles and Protocols. 2010. p. 85-97. (Methods in Molecular Biology). doi: 10.1007/978-1-60761-746-4_4