The Tufted Titmouse's song is a fast-repeated, clear whistle: peter-peter-peter. The birds repeat this up to 11 times in succession or up to 35 songs delivered per minute. Females occasionally sing a quieter version of the song.
Titmouse calls are nasal and mechanical. A scratchy, chickadee-like tsee-day-day-day is the most common. Tufted Titmice also give fussy, scolding call notes and, when predators are sighted, a harsh distress call that warns other titmice of the danger.