Development of Cross-specific Microsatellite Markers for Echinochloa spp. (Poaceae)
Jeongran Lee1,* Jin-Won Kim1 In-Yong Lee1
Echinochloa spp. is one of the most problematic weeds, especially in rice fields. Therefore, most of the studies on this genus have been focused on chemical control. Its genetic diversity using molecular markers was not widely studied because of a few markers available. Microsatellite markers were developed in this study to evaluate the genetic diversity of a cross-specific troublesome weed Echinochloa species (Poaceae). Illumina sequencing identified a total of 44,257 di-/tri-nucleotide repeat motifs. Finally, thirteen microsatellite markers were identified for Echinochloa crus-galli. All markers were polymorphic and cross amplified in E. oryzicola. The average number of alleles per locus was 8.0 and polymorphism information content was 0.611. Observed and expected heterozygosity ranged from 0.00 to 0.98 and from 0.06 to 0.86, respectively. The 13 polymorphic markers identified in this study will be useful for assessing the genetic diversity of E. crus-galli along with E. oryzicola and for predicting the dispersal pattern of these weeds in agricultural fields.
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