Characterization of the Bhanja serogroup viruses (bunyaviridae): A novel species of the genus phlebovirus and its relationship with other emerging tick-borne phleboviruses
Keita Matsuno, Keita Weisend, Amelia P A Travassos da Rosa, Sarah L. Anzick, Eric Dahlstrom, Stephen F. Porcella, David W. Dorward, Xue Jie Yu, Robert B. Tesh, Hideki Ebihara
Bhanja virus (BHAV) and its antigenically close relatives Forecariah virus (FORV), Kismayo virus (KISV), and Palma virus (PALV) are thought to be members of the family Bunyaviridae, but they have not been assigned to a genus or species. Despite their broad geographical distribution and reports that BHAV causes sporadic cases of febrile illness and encephalitis in humans, the public health importance of the Bhanja serogroup viruses remains unclear, due in part to the lack of sequence and biochemical information for the virus proteins. In order to better define the molecular characteristics of this group, we determined the full-length sequences of the L, M, and S genome segments of multiple isolates of BHAV as well as FORV and PALV. The genome structures of these Bhanja viruses are similar to those of viruses belonging to the genus Phlebovirus. Functional domains and amino acid motifs in the viral proteins that are conserved among other known phleboviruses were also identified in proteins of the BHAV group. Phylogenetic and serological analyses revealed that the BHAVs are most closely related to the novel emerging tick-borne phleboviruses severe fever with thrombocytopenia syndrome virus and Heartland virus, which have recently been implicated as causing severe acute febrile illnesses associated with thrombocytopenia in humans in China and the United States. Our results indicate that the Bhanja serogroup viruses constitute a single novel species in the genus Phlebovirus. The results of this study should facilitate epidemiological surveillance for other, similar tick-borne phleboviruses that may represent unrecognized causes of febrile illness in humans.
Phlebovirus
Bunyaviridae
serotypes
Serogroup
amino acid motifs
Matsuno, K., Weisend, K., Travassos da Rosa, A. P. A., Anzick, S. L., Dahlstrom, E., Porcella, S. F., ... Ebihara, H. (2013). Characterization of the Bhanja serogroup viruses (bunyaviridae): A novel species of the genus phlebovirus and its relationship with other emerging tick-borne phleboviruses. Journal of Virology, 87(7), 3719-3728. https://doi.org/10.1128/JVI.02845-12
Characterization of the Bhanja serogroup viruses (bunyaviridae) : A novel species of the genus phlebovirus and its relationship with other emerging tick-borne phleboviruses. / Matsuno, Keita; Weisend, Keita; Travassos da Rosa, Amelia P A; Anzick, Sarah L.; Dahlstrom, Eric; Porcella, Stephen F.; Dorward, David W.; Yu, Xue Jie; Tesh, Robert B.; Ebihara, Hideki.
In: Journal of Virology, Vol. 87, No. 7, 04.2013, p. 3719-3728.
Matsuno, K, Weisend, K, Travassos da Rosa, APA, Anzick, SL, Dahlstrom, E, Porcella, SF, Dorward, DW, Yu, XJ, Tesh, RB & Ebihara, H 2013, 'Characterization of the Bhanja serogroup viruses (bunyaviridae): A novel species of the genus phlebovirus and its relationship with other emerging tick-borne phleboviruses', Journal of Virology, vol. 87, no. 7, pp. 3719-3728. https://doi.org/10.1128/JVI.02845-12
Matsuno K, Weisend K, Travassos da Rosa APA, Anzick SL, Dahlstrom E, Porcella SF et al. Characterization of the Bhanja serogroup viruses (bunyaviridae): A novel species of the genus phlebovirus and its relationship with other emerging tick-borne phleboviruses. Journal of Virology. 2013 Apr;87(7):3719-3728. https://doi.org/10.1128/JVI.02845-12
Matsuno, Keita ; Weisend, Keita ; Travassos da Rosa, Amelia P A ; Anzick, Sarah L. ; Dahlstrom, Eric ; Porcella, Stephen F. ; Dorward, David W. ; Yu, Xue Jie ; Tesh, Robert B. ; Ebihara, Hideki. / Characterization of the Bhanja serogroup viruses (bunyaviridae) : A novel species of the genus phlebovirus and its relationship with other emerging tick-borne phleboviruses. In: Journal of Virology. 2013 ; Vol. 87, No. 7. pp. 3719-3728.
@article{5528986bec974cb8b59c8c6c1ea90e5a,
title = "Characterization of the Bhanja serogroup viruses (bunyaviridae): A novel species of the genus phlebovirus and its relationship with other emerging tick-borne phleboviruses",
abstract = "Bhanja virus (BHAV) and its antigenically close relatives Forecariah virus (FORV), Kismayo virus (KISV), and Palma virus (PALV) are thought to be members of the family Bunyaviridae, but they have not been assigned to a genus or species. Despite their broad geographical distribution and reports that BHAV causes sporadic cases of febrile illness and encephalitis in humans, the public health importance of the Bhanja serogroup viruses remains unclear, due in part to the lack of sequence and biochemical information for the virus proteins. In order to better define the molecular characteristics of this group, we determined the full-length sequences of the L, M, and S genome segments of multiple isolates of BHAV as well as FORV and PALV. The genome structures of these Bhanja viruses are similar to those of viruses belonging to the genus Phlebovirus. Functional domains and amino acid motifs in the viral proteins that are conserved among other known phleboviruses were also identified in proteins of the BHAV group. Phylogenetic and serological analyses revealed that the BHAVs are most closely related to the novel emerging tick-borne phleboviruses severe fever with thrombocytopenia syndrome virus and Heartland virus, which have recently been implicated as causing severe acute febrile illnesses associated with thrombocytopenia in humans in China and the United States. Our results indicate that the Bhanja serogroup viruses constitute a single novel species in the genus Phlebovirus. The results of this study should facilitate epidemiological surveillance for other, similar tick-borne phleboviruses that may represent unrecognized causes of febrile illness in humans.",
author = "Keita Matsuno and Keita Weisend and {Travassos da Rosa}, {Amelia P A} and Anzick, {Sarah L.} and Eric Dahlstrom and Porcella, {Stephen F.} and Dorward, {David W.} and Yu, {Xue Jie} and Tesh, {Robert B.} and Hideki Ebihara",
T1 - Characterization of the Bhanja serogroup viruses (bunyaviridae)
T2 - A novel species of the genus phlebovirus and its relationship with other emerging tick-borne phleboviruses
AU - Matsuno, Keita
AU - Weisend, Keita
AU - Travassos da Rosa, Amelia P A
AU - Anzick, Sarah L.
AU - Dahlstrom, Eric
AU - Porcella, Stephen F.
AU - Dorward, David W.
AU - Yu, Xue Jie
AU - Tesh, Robert B.
AU - Ebihara, Hideki
N2 - Bhanja virus (BHAV) and its antigenically close relatives Forecariah virus (FORV), Kismayo virus (KISV), and Palma virus (PALV) are thought to be members of the family Bunyaviridae, but they have not been assigned to a genus or species. Despite their broad geographical distribution and reports that BHAV causes sporadic cases of febrile illness and encephalitis in humans, the public health importance of the Bhanja serogroup viruses remains unclear, due in part to the lack of sequence and biochemical information for the virus proteins. In order to better define the molecular characteristics of this group, we determined the full-length sequences of the L, M, and S genome segments of multiple isolates of BHAV as well as FORV and PALV. The genome structures of these Bhanja viruses are similar to those of viruses belonging to the genus Phlebovirus. Functional domains and amino acid motifs in the viral proteins that are conserved among other known phleboviruses were also identified in proteins of the BHAV group. Phylogenetic and serological analyses revealed that the BHAVs are most closely related to the novel emerging tick-borne phleboviruses severe fever with thrombocytopenia syndrome virus and Heartland virus, which have recently been implicated as causing severe acute febrile illnesses associated with thrombocytopenia in humans in China and the United States. Our results indicate that the Bhanja serogroup viruses constitute a single novel species in the genus Phlebovirus. The results of this study should facilitate epidemiological surveillance for other, similar tick-borne phleboviruses that may represent unrecognized causes of febrile illness in humans.
AB - Bhanja virus (BHAV) and its antigenically close relatives Forecariah virus (FORV), Kismayo virus (KISV), and Palma virus (PALV) are thought to be members of the family Bunyaviridae, but they have not been assigned to a genus or species. Despite their broad geographical distribution and reports that BHAV causes sporadic cases of febrile illness and encephalitis in humans, the public health importance of the Bhanja serogroup viruses remains unclear, due in part to the lack of sequence and biochemical information for the virus proteins. In order to better define the molecular characteristics of this group, we determined the full-length sequences of the L, M, and S genome segments of multiple isolates of BHAV as well as FORV and PALV. The genome structures of these Bhanja viruses are similar to those of viruses belonging to the genus Phlebovirus. Functional domains and amino acid motifs in the viral proteins that are conserved among other known phleboviruses were also identified in proteins of the BHAV group. Phylogenetic and serological analyses revealed that the BHAVs are most closely related to the novel emerging tick-borne phleboviruses severe fever with thrombocytopenia syndrome virus and Heartland virus, which have recently been implicated as causing severe acute febrile illnesses associated with thrombocytopenia in humans in China and the United States. Our results indicate that the Bhanja serogroup viruses constitute a single novel species in the genus Phlebovirus. The results of this study should facilitate epidemiological surveillance for other, similar tick-borne phleboviruses that may represent unrecognized causes of febrile illness in humans.