Reading: Building a Tissue In Vitro from the Bottom Up: Implications in Regenerative Medicine
Building a Tissue In Vitro from the Bottom Up: Implications in Regenerative Medicine
Francesco Urciuolo ,
Center for Advanced Biomaterials for HealthCare at CRIB Istituto Italiano di Tecnologia, Naples; Interdisciplinary Research Center on Biomaterials (CRIB), University of Naples Federico II, Naples, IT
Giorgia Imparato,
About Giorgia
Alessandra Totaro,
About Alessandra
Paolo A. Netti
About Paolo A.
Tissue engineering aims at creating biological tissues to improve or restore the function of diseased or damaged tissues. To enhance the performance of engineered tissues, it is required to recapitulate in vitro not only the composition but also the structural organization of native tissues. To this end, tissue engineering techniques are beginning to focus on generating micron-sized tissue modules having specific microarchitectural features that can be used alone as living filler in the damaged areas or serve as building blocks to engineer large biological tissues by a bottom-up approach. This work discusses the shortcomings related to traditional "top-down" strategies and the promises of emerging ''bottom-up" approaches in creating engineered biological tissues. We first present an overview of the current tissue-building techniques and their applications, with an analysis of the potentiality and shortcomings of different approaches. We then propose and discuss a novel method for the biofabrication of connective-like micro tissues and how this technique can be translated to cardiac muscle fabrication.
Keywords: tissue engineering, dermis, equivalent, cardiac muscle, bottom-up strategy, regenerative medicine, bioreactor
How to Cite: 1. Urciuolo F, Imparato G, Totaro A, Netti PA. Building a Tissue In Vitro from the Bottom Up: Implications in Regenerative Medicine. Methodist DeBakey Cardiovascular Journal. 2013;9(4):213-217. DOI: http://doi.org/10.14797/mdcj-9-4-213
Urciuolo, F., Imparato, G., Totaro, A. and Netti, P.A., 2013. Building a Tissue In Vitro from the Bottom Up: Implications in Regenerative Medicine. Methodist DeBakey Cardiovascular Journal, 9(4), pp.213–217. DOI: http://doi.org/10.14797/mdcj-9-4-213
Urciuolo F, Imparato G, Totaro A, Netti PA. Building a Tissue In Vitro from the Bottom Up: Implications in Regenerative Medicine. Methodist DeBakey Cardiovascular Journal. 2013;9(4):213–7. DOI: http://doi.org/10.14797/mdcj-9-4-213
Urciuolo, F., Imparato, G., Totaro, A., & Netti, P. A. (2013). Building a Tissue In Vitro from the Bottom Up: Implications in Regenerative Medicine. Methodist Debakey Cardiovascular Journal, 9(4), 213–217. DOI: http://doi.org/10.14797/mdcj-9-4-213
1. Urciuolo F, Imparato G, Totaro A, Netti PA. Building a Tissue In Vitro from the Bottom Up: Implications in Regenerative Medicine. Methodist DeBakey Cardiovascular Journal. 2013;9(4):213-217. DOI: http://doi.org/10.14797/mdcj-9-4-213
Urciuolo F and others, 'Building a Tissue in Vitro from the Bottom Up: Implications in Regenerative Medicine' (2013) 9 Methodist DeBakey Cardiovascular Journal 213 DOI: http://doi.org/10.14797/mdcj-9-4-213
Urciuolo, Francesco, Giorgia Imparato, Alessandra Totaro, and Paolo A. Netti. 2013. "Building a Tissue in Vitro from the Bottom Up: Implications in Regenerative Medicine". Methodist Debakey Cardiovascular Journal 9 (4): 213–17. DOI: http://doi.org/10.14797/mdcj-9-4-213
Urciuolo, Francesco, Giorgia Imparato, Alessandra Totaro, and Paolo A. Netti. "Building a Tissue in Vitro from the Bottom Up: Implications in Regenerative Medicine". Methodist Debakey Cardiovascular Journal 9, no. 4 (2013): 213–17. DOI: http://doi.org/10.14797/mdcj-9-4-213
Urciuolo, F, et al.. "Building a Tissue In Vitro from the Bottom Up: Implications in Regenerative Medicine". Methodist Debakey Cardiovascular Journal, vol. 9, no. 4, 2013, pp. 213–17. DOI: http://doi.org/10.14797/mdcj-9-4-213