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You are an expert at identifying data availability statements in scientific text. A data availability statement describes where research data can be found or how it can be accessed.<|eot_id|><|start_header_id|>user<|end_header_id|>

Determine if the following text contains a data availability statement. Answer with 'yes' or 'no'.

Text: To determine if ANXA11 co - localized with co - motile RNA granules and lysosomes in axons , we performed time - lapse imaging of cultured rodent neurons . Similar to stress granules , ANXA11 co - localized and co - trafficked with motile RNA granule / lysosome assemblies in axons ( Figure 6F , Video S4 ) . ( E ) Quantification of integrated fluorescence intensity of Opto - labeled proteins in ( D ) during 300 seconds of 488 nm light activation , n=20 . Error bars = SEM . See also Figure S4C. ( F ) Quantification of the number of Opto - labeled puncta present 30 minutes after the 488 nm light was turned off . n=17 - 19 . One - way ANOVA , ns , not significant . * * * * p < 0.0001 . Error bars= SEM . ( G ) Immunostaining of mEmerald - tagged wild - type and mutant ANXA11 with G3BP1 and mRNA labeled by Oligo - dT in U2OS cells following 30 minutes of heat shock . Co - localization of ANXA11 with individual RNA granules is plotted in the line scans to the right . Scale bar : 30 mm . See also Figure S4D. ( H ) Quantification of area of ANXA11 structures co - localizing with G3BP1 - labeled RNA granules in ( G ) . n=28 - 31 . One - way ANOVA , ns , not significant . * * * * p < 0.0001 . Error bars = SEM . We then asked if ANXA11 was necessary to facilitate axonal RNA granule / lysosomal hitchhiking . ANXA11 knockdown in primary rodent neurons ( Figure S6D ) substantially impaired axonal RNA granule / lysosome hitchhiking ( Figures 6 G and 6H ) without altering axonal lysosome transport itself ( Figure S6E ) . To determine if ANXA11 knockdown impaired RNA delivery to distal regions of the cell , we quantified levels of actin mRNA in growth cones using single molecule FISH ( smFISH ) . We found that ANXA11 knockdown reduced levels of actin mRNA in growth cones , consistent with impaired long - distance axonal mRNA transport ( Figures 6I and 6J ) . Together , these data indicate that ANXA11 is sufficient to facilitate tethering of RNA granules to lysosomes , and is necessary for axonal RNA granule hitchhiking and delivery of mRNA to distal locations within neurons .<|eot_id|><|start_header_id|>assistant<|end_header_id|>

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