<|begin_of_text|><|start_header_id|>system<|end_header_id|>

You are an expert at identifying data availability statements in scientific text. A data availability statement describes where research data can be found or how it can be accessed.<|eot_id|><|start_header_id|>user<|end_header_id|>

Determine if the following text contains a data availability statement. Answer with 'yes' or 'no'.

Text: DNA sequence analysis . Genomic DNA was isolated from lymphoblastoid cell lines of the patient , the healthy sibling and the parents . WGS was performed at Complete Genomics Inc. 6 ( CG ) using their standard sequencing pipeline , version 2.0.2.26 . For further analysis only small variants ( single - nucleotide polymorphisms and small insertions , deletions and block substitutions ) from the CG ' var ' file were considered , which reports variants compared with the reference genome ( NCBI build 37.2 ) up to a length of about 50 nucleotides . CGAtools ( CG Analysis Tools ) version 1.5 was used as provided by CG ( http://cgatools.sourceforge.net ) for subsequent analysis . With an in - house WGS analysis pipeline , we searched for recessive , compound heterozygous and de novo variants ( Fig . 2 ) . First , we combined all variants from all genomes of every family member into the union of variants using the CGAtools listvariant command and CG ' var ' files as input . Then , we used the CGAtools testvariant command to test each genome for the presence of each allele at every variant position . We next applied the following filtering steps : ( 1 ) we removed variants that were not called in at least one genome as high - quality calls ( VQHIGH ) by CG . ( 2 ) Next , we filtered for rare variants having a minor allele frequency equal or smaller than 1 % in the European American population of the 1000genomes project 36 and the NHLBI ESP exomes ( https://esp.gs.washington.edu ) , as well as in the control data set CG69 provided by CG . ( 3 ) Variants were filtered out , for which in the index patient both alleles were called reference . If one or both alleles of the index patient were not called , the variant was not filtered out at this stage . ( 4 ) When applying a recessive inheritance model , we filtered for variants that were either called homozygous in the index patient and not in the unaffected sibling and heterozygous in the parents or compound heterozygous allowing for no call alleles . Here we filtered for two different heterozygous variants within the same gene coming from different parental genomes and both not present in the unaffected sibling . ( 5 ) Variants were annotated using ANNOVAR 37 using the NCBI RefSeq Release 60 and Ensembl Release 74 genome annotations . Only exonic variants were kept that were annotated as non - synonymous , frameshift insertion / deletion , nonframeshift insertion / deletion / substitution , stop gain , stop loss and variants disrupting splice sites up to two bases into intron or exon . The remaining variants were then assessed using SIFT 38 , Polyphen 39 , MutationTaster 40 and CADD 41 for pathogenicity . Ingenuity Variant Analysis ( Qiagen ) and the Family Genomics Workflow ( familygenomics.systemsbiology.net/software ) provided independent candidate lists , and were used for confirmation of the primary analysis . Wholeexome sequencing was performed after enrichment with SureSelect Human All Exon Kit ( Agilent ) targeting B38 Mb , and sequenced on one lane of an Illumina GAIIx instrument using a paired - end sequencing protocol . To detect and evaluate SNVs and small indels , reads were mapped to the GRCh37 reference genome ( hg19 ) by the Burrows - Wheeler Aligner 42 . Subsequently , SNVs and small indels were called using SAMtools 43 and VarScan 44 . The resulting list of variants was annotated with ANNOVAR . Finally , manual inspection of relevant variants was carried out by visualizing the mapped reads in the Integrative Genome Browser ( IGV ) 45 .<|eot_id|><|start_header_id|>assistant<|end_header_id|>

yes<|eot_id|>