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You are an expert at identifying data availability statements in scientific text. A data availability statement describes where research data can be found or how it can be accessed.<|eot_id|><|start_header_id|>user<|end_header_id|>

Determine if the following text contains a data availability statement. Answer with 'yes' or 'no'.

Text: In this communication , the contribution of Asp151 , Asp213 and Asp214 , constituting the triad of conserved carboxylates at the DNA polymerase active site Ty3 RT , was evaluated . Since analogous mutants within the active site of HIV-1 RT have a severe effect on DNA polymerase activity ( 34 ) , it has been assumed that a precise geometry of the three carboxylates in the catalytic center is absolutely essential . Our results with Ty3 RT suggest more relaxed structural constrains for this catalytic triad . We demonstrate here that while substituting Asn for Asp151 and Asp213 completely disrupts Mg + + dependent activities , Asn and Glu can replace Asp214 . In contrast , an Asp214!Gln mutation dramatically decreases catalytic activity . The Asp151!Glu mutation also has severe consequences for RNase H activity , suggesting close contact between the catalytic centers of Ty3 RT . In several instances , including mutant Asp151!Glu , metal rescue of DNA polymerase activity could be achieved by substitution of Mn + + for Mg + + . Finally , although recombinant RTs containing Asn or Glu substitutions for Asp214 retain DNA polymerase function , these mutations are lethal for Ty3 transposition , which our data suggest may be due to reduced processivity during minus - strand , RNA - dependent DNA synthesis .<|eot_id|><|start_header_id|>assistant<|end_header_id|>

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