<|begin_of_text|><|start_header_id|>system<|end_header_id|>

You are an expert at identifying data availability statements in scientific text. A data availability statement describes where research data can be found or how it can be accessed.<|eot_id|><|start_header_id|>user<|end_header_id|>

Determine if the following text contains a data availability statement. Answer with 'yes' or 'no'.

Text: days when stored at 4 ° C in electrolyte ) along with their complex and low - yield synthesis have contributed to impair commercialization and clinical adaptation of biosensors due to obstacles such as improper handling , transportation and storage , and high cost . 16 Protein subunits such as receptor - binding domain ( RBD ) and peptides ( 5 to 15 amino acids ) have been devised as promising solutions to cope with the issues mentioned above of biosensors loading big - molecule bioreceptors . Cai et al . 17 used a peptide - loaded assay for the chemiluminescence detection of COVID-19 IgG and IgM. The peptides can be synthetically synthesized via reproducible routines , and they are also not amenable to denaturation , showing mechanical and thermal stabilities . To date , these subunits can maintain their " one - lock , one - key " interaction ability with ligands even at high temperatures ( e.g. , > 100 ° C ) . 18 Therefore , natureinspired peptides identified from structural analyses and computational modeling to mimic biomolecule epitopes have garnered considerable interest in developing the nextgeneration class of POC biosensors . 11,16 To address the gaps in the current state - of - the - art biosensing technologies as discussed before , we here developed an LF - EIS immunosensor based on a peptide mimicking the N - terminal domain ( NTD ) in S protein ( it is located on the N - terminal subunit , S1 ) 11 for sensing anti - SARS - CoV-2 S protein Ab ( abbreviated herein as Ab S ) . Our goal is to generate a straightforward , fast , and accurate COVID-19 screening method loading a stable ( long shelf life ) , easily synthesized , and cost - effective recognition element . Screening of patient sera and plasma was successfully reached by taking advantage of ( 1 ) bioreceptor - related features , ( 2 ) advances in surface chemistry , and ( 3 ) high accuracy , which are key to bring the devices into mainstream use . ( 1 ) Inspired by nature to mimic anti - Ab S binding , the peptide ( abbreviated as PEP2003 ) was used to produce a glassy carbon - based LF - EIS biosensor . Originated from a no template control ( NTC ) portion of the S protein NTD , such a peptide was gleaned from phagolysosomal proteolysis using a virtual proteolytic cleavage tool . 19,20 PEP2003 avoids denaturation risks , and it can be chemically synthesized . 21 ( 2 ) This peptide was physiosorbed on a glassy carbon electrode ( GCE ) coated with gold nanoparticle ( AuNPs ) through a fast routine ( 30 min ) . In contrast to covalent functionalization , physical adsorptions avoid the sp 2 − sp 3 rehybridization of carbon , thus preserving its conductivity . 21 Such a process further can be easily tailored to sense other targets from a simple , fast , and universal drop - casting In the latter case , the colors in red , blue , and green mean oxygen , nitrogen , and carbon , respectively . The bottom image shows the primary structure ( amino acids 1 to 1273 ) of the SARS - CoV-2 S protein 45 with indication ( green arrow ) of the PEP2003 position in the NTD ( between the amino acids 121 and 125 : Asn - Asn - Ala - Thr - Asn - COOH ) . SS , S2′ , FP , HR1 , CH , CD , HR2 , TM , and CT mean , respectively , signal sequence , protease cleavage site , fusion peptide , heptad repeat 1 , central helix , connector domain , heptad repeat 2 , transmembrane domain , and cytoplasmic tail , whereas the black arrows indicate protease cleavage sites . ( C ) Enhanced dark - field microscopy−hyperspectral images of AuNPs ( 1 ) and AuNP / PEP2003 ( AuNP / Pep ) conjugates ( 2 ) and the resulting average spectra ( 3 ) and PCA plots ( 4 ) . ( D ) Enhanced dark - field microscopy−hyperspectral images of AuNPs / Pep after interaction with control sample ( CS ; 1 ) and positive serum ( PS ; 2 ) and the resulting average spectra ( 3 ) and PCA plots ( 4 ) . In ( C ) and ( D ) , the data colors in ( 3 ) are valid in ( 4 ) . step . ( 3 ) PEP2003 was impressively found to generate a sensitivity higher than the full - length S protein ( Scheme 1 ) , which is supported by the increase in the target - to - receptor size ( TRS ) ratio . 13 This high sensitivity was key to ensure accurate COVID-19 screening by allowing a high dilution of the samples ( 500 - fold ) . In addition to delivering the analysis of small - volume samples ( 12.0 nL ) , this step inhibits the nonspecific adsorption of proteins ( biofouling ) as the interferences present in the biofluids are diluted to negligible contents , 8 preventing the occurrence of false positives .<|eot_id|><|start_header_id|>assistant<|end_header_id|>

no<|eot_id|>