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You are an expert at identifying data availability statements in scientific text. A data availability statement describes where research data can be found or how it can be accessed.<|eot_id|><|start_header_id|>user<|end_header_id|>

Determine if the following text contains a data availability statement. Answer with 'yes' or 'no'.

Text: SMARTseq - v4 sequencing data were aligned with hisat2 ( ref . 68 ) ( v4.8.2 ) , using the genome reference mm10 . We used featureCounts 69 ( v1.6.4 ) to calculate read counts . The quality of cells was then assessed based on the number of total counts per cell ( library size ) , requiring at least 500,000 reads per cell . A total of 84 AML and 43 stroma cells were retained for downstream analysis . Seurat was used to analyze the SMARTseq - v4 data , and the quality parameters are listed in Supplementary Table 7 and Extended Data Fig . 5d , e. In brief , we first performed data normalization using the NormalizeData function with default settings . The top 30 principal components were used to construct shared nearest neighbors graphs and UMAP embeddings . The FindAllMarkers function from Seurat was used to identify differentially expressed ( marker ) genes for clusters or subtypes . Mesenchymal stromal and endothelial cells were annotated using well - established marker genes : for mesenchymal stromal cells the marker genes were Cxcl12 , Pcolce , Ogn and Adipoq ; and for the endothelial cells the markers were Eng , Kdr , Plvap and Egfl7 ( see also the heatmap in Extended Data Fig . 6d , as well as ref . 27 ) .<|eot_id|><|start_header_id|>assistant<|end_header_id|>

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