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Text: The antioxidant activity of galangin was determined based on its superoxide radical scavenging ability that was measured using a protocol developed in our lab [22]. In this cyclic voltammetry method superoxide is developed in situ by bubbling O 2 into the electrovoltaic cell containing anhydrous DMSO solvent, see details in Experimental section. After the blank experiment, aliquots taken from a stock 0.02M galangin solution, were added to the voltaic cell. Fig 10A shows voltammograms of blank and all runs for both electrodes. The lower part of these voltammograms develops at the disk electrode, where O 2 incorporates an electron to generate superoxide O 2 • -. The opposite effect, oxidation of O 2 • -, takes place at the ring electrode, which has a fixed potential enough positive for this action, upper part. As scavenger aliquots are added, they consume part of the superoxide and the ring disc detects less superoxide, lowering the current. The collection efficiency is shown in Fig 10B, for all data, showing the decrease of superoxide. All scavengers studied by us so far behaved in a linear manner for the initial aliquots used. Beyond the initial aliquots, 2 possibilities were seen: (1) complete linear behavior, as in, chrysin [22], eriodictyol [22], BHT [31], emodin [32], celastrol [34] or (2) initial linear behavior followed by asymptotic performance, such as in quercetin [22], embelin [31] and clovamide [35]. In the present study, we also calculated a linear trend for all data, with regression factor of 0.9296, whereas the initial 3 **data** (using the blank plus 2 initial added aliquots) have a linear regression factor of 0.9635, slightly better, but still not ideal. Since we find that several superoxide anions are incorporated in one galangin unit, Fig 9C, galangin is a very efficient scavenger, and indeed this is demonstrated by the slope in Fig 10B, -19x10 4 . In <|eot_id|><|start_header_id|>assistant<|end_header_id|>

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