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Text: Abundance scores correlated modestly well between replicates ( mean Pearson 's r = 0.63 , mean Spearman 's ρ = 0.62 for PTEN ; and mean r = 0.73 , mean ρ = 0.67 for TPMT ; Supplementary Fig . 2 ) . To improve accuracy , final abundance scores and confidence intervals were computed from eight replicate experiments . The resulting data set describes the effects of 4,112 of the 7,638 possible single amino acid PTEN variants and 3,689 of the 4,655 possible TPMT variants ( Fig . 2c , Table   2 ) . VAMP - seq - derived abundance scores were highly correlated with the abundances of protein variants assessed in individual experiments ( n = 25 , r = 0.96 , ρ = 0.96 for PTEN ; n = 19 , r = 0.75 , ρ = 0.61 for TPMT ; Supplementary Fig . 3a , b ) . Furthermore , PTEN variant abundance measured using full - length EGFP or a fifteen amino acid split - GFP tag 19 were in agreement ( n = 6 , r = 0.98 , ρ = 0.94 ; Supplementary Fig . 1d ) . Finally , our abundance scores were consistent with 41 PTEN and 20 TPMT variant abundance effects assessed by western blotting ( Supplementary Fig . 3c , d ) . Thus , VAMP - seq accurately quantifies steady - state protein variant abundance .<|eot_id|><|start_header_id|>assistant<|end_header_id|>

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