Title: White Blood Cell Differential Count using Wright Stain
The objective of the experiment was to prepare a fresh blood smear and distinguish different types of blood cells using wright’s stains.
Blood is an important part of a human body. Blood is the fluid in our body. The blood contains cellular elements. The cellular elements are serum and plasma. Serum is the blood fluid after thrombin and other clotting factors have been removed. Plasma is the blood fluid with the clotting factors and other substances in it. There are two types of blood cells. They are Erythrocytes and leukocytes.
Erythrocytes or Red blood Cells main purpose is to transport oxygen throughout the cells of the body. Oxygen binds to hemoglobin. Hemoglobin is an iron-containing protein, which facilitates transportation of oxygen by reversibly binding to this respiratory gas and greatly increasing its solubility in blood. Arterial blood carries oxygen from inhaled air to the tissues of the body, and venous blood carries carbon dioxide, a waste product of metabolism produced by cells, from the tissues to the lungs to be exhaled.
Leukocytes or white blood cells are responsible for resisting infections that enter the body. There are five types of white blood cells. They are neutrophils, basophils, eosinophils, monocytes, and lymphocytes. Each blood cell protects the body from specific infections. They can be identified by their different morphology. Some have noticeable granules whereas others don’t. WBC’s are placed into two different categories. The WBC’s with visible granular are called granular whereas WBC’s without visible granular are called a-granular. WBC’s that are a-granular include lymphocytes and monocytes. Granular WBC’s include neutrophils, basophils, and eosinophils.
Neutrophils make up to 50-70% of all of the white blood cells. It is the most abundant white blood cell. It is highly phagocytic during the infection process. It is usually...