By a News Reporter-Staff News Editor at Cancer Gene Therapy Week -- Research findings on Biotechnology are discussed in a new report. According to news reporting from Guangdong, People's Republic of China, by NewsRx journalists, research stated, "Gene therapy is a promising therapeutic method but is severely hampered due to its lack of an ideal delivery system. Therefore, in this study, a nonviral and magnetic resonance imaging (MRI) visible vector, polyethylene glycol-grafted polyethylenimine and superparamagnetic iron oxide nanoparticles (PEG-g-PEI-SPION) was used as a nanocarrier for small interfering RNA (siRNA) delivery in gastric cancer."
The news correspondents obtained a quote from the research from Sun Yat-Sen University, "Biophysical characterization of PEG-g-PEI-SPION was systematically analyzed, including size, zeta potential, siRNA condensation capacity, cell viability, transfection efficiency, cellular uptake, and MRI-visible function in vivo. Besides, CD44 variant isoform 6 (CD44v6), a protein marker for metastatic behavior in gastric cancer, and was chose as the target gene to further analyze the siRNA delivery function of PEG-g-PEI-SPION. Under comprehensive analysis, the appropriate N/P ratio of PEG-g-PEI-SPION/siRNA was 10,. and siRNA targeting at human CD44v6 (siCD44v6) transferred by PEG-g-PEI-SPION was effective at downregulating the CD44v6 expression of gastric carcinoma cell line SGC-7901 in vitro. Moreover, knockdown of CD44v6 impaired migrating and invasive abilities of SGC-7901 cells. Furthermore, PEG-g-PEI-SPION was a highly efficient contrast agent for MRI scan in vivo. PEG-g-PEI-SPION was a promising nonviral vector with molecular image tracing capacity for cancer gene therapy."
According to the news reporters, the research concluded: "And CD44v6 was a potential target gene for the prevention and detection of metastatic behavior in gastric cancer."
For more information on this research see: Characterization of polyethylene glycol-grafted polyethylenimine and superparamagnetic iron oxide nanoparticles (PEG-g-PEI-SPION) as an MRI-visible vector for siRNA delivery in gastric cancer in vitro and in vivo. Journal of Gastroenterology, 2013;48(7):809-821. Journal of Gastroenterology can be contacted at: Springer Japan Kk, Chiyoda First Bldg East, 3-8-1 Nishi-Kanda, Chiyoda-Ku, Tokyo, 101-0065, Japan. (Springer - www.springer.com; Journal of Gastroenterology - www.springerlink.com/content/0944-1174/)
Our news journalists report that additional information may be obtained by contacting Y.T. Chen, Sun Yat Sen UniversitySch Chem & Chem Engn, Center Biomed Engn, Guangzhou 510275, Guangdong, People's Republic of China. Additional authors for this research include G.D. Lian, C.D. Liao, W.W. Wang, L.J. Zeng, C.C. Qian, K.H. Huang and X.T. Shuai (see also technology.html">Biotechnology).
Keywords for this news article include: Asia, Biotechnology, siRNA, Alkenes, Genetics, Oncology, Polyenes, Guangdong, Hydrocarbons, Nanoparticle, Therapeutics, Bioengineering, Gastric Cancer, Nanotechnology, Gastroenterology, Organic Chemicals, Cancer Gene Therapy, Polyethylene Glycols, Emerging Technologies, Small Interference RNAs, People's Republic of China
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