* For research use only, not for use in diagnostic procedures.
Principle Of The Test
The Dengue NS1 Rapid test(Strip) is a qualitative,membrane based immunoassay for the detection of NS1 antigen in human serum.The rapid test membrane is precoated with a NS1 specific antibody on the test line region and utilizes a separate control to assure assay flow and performance.During testing,the test sample is added directly to the sample region and the test is placed into a well containing 3 drops of buffer. The buffer and serum mix and interact with NS1-specific monoclonal antibodies conjugated to gold nanoparticles.The solution migrates upward on the membrane (via capillary action)to react with the anti-NS1 antibody on the membrane. If NS1 antigen is present,a red line will appear at the test line.The red line at the control region should always appear if the assay is performed correctly.The presence of this red line verifies that proper flow has occurred and catastrophic failure of the conjugate has not occurred.The entire procedure takes approximately 30 minutes.
Reagents And Materials Provided1.Twenty-five Dengue NS1 Rapid Test dipsticks,individually pouched or 25 test strips in a vial with desiccant in the cap.Store at room temperature in vial.
2.One vial of Chase Buffer Type A,6ml.Store at room temperature.