Most bacteria are able to communicate with each other by secreting signaling molecules. Once the concentration of signals has reached a critical density (the Quorum), the bacteria are able to coordinate their behavior. Only when this critical population density has been reached certain genes are activated that lead to, for example, the formation of biofilms or the expression of virulence factors. Bacteria utilize this so-called Quorum Sensing (QS) to synchronize their behavior to regulate functions that benefit the entire population.
The most commonly used signaling molecules are N-Acyl-L-homoserine lactones (AHLs) that are secreted by the bacteria into their surroundings, where they can easily be incorporated by other cells. The AHLs then start binding to specific QS-receptors once a certain density has been reached inside the cell.
Fluorescent labeling of signaling compound to visualize receptors
The research groups under the leadership of Prof. Karl Gademann (University of Basel) and Prof. Leo Eberl (University of Zurich) have succeeded in visualizing live cell-to-cell communication pathways. The scientists added fluorescents tags to natural AHL signaling molecules and were able to show through tests with bacterial cultures that the modified signaling molecule selectively binds to the Burkholderia cenocepacia QS receptor.
B. cenocepacia is a member of a bacterial group known to form biofilms in the lungs of immunocompromised persons or patients suffering from cystic fibrosis, causing severe complications such as pneumonia.
The scientists were also able to detect the receptor in a native population of B. cenocepacia. Here, the natural AHL signaling molecule is competing with its artificial analogue for the binding to the receptor. The fluorescent-labeling agent was equally distributed over the live cell, which made it possible to localize the receptor inside the cytoplasm for the first t
|Contact: Reto Caluori|
University of Basel