We have spent the last 3 days learning about sequencing, alignment, creating trees, the algorithms and the role of probability in the program’s used to create the trees and to grade their reliability.
So basically maths is vital to the creation of the trees.
Last day with Graham and without any help we have to Search the database for a sequence of choice, BLAST the sequence and select organisms with listed that match somewhat our target.
We align the sequences using Clustal, import the alignment into MEGA(programme that will create the tree), select the model that will create the most mathematically reliable model and create the tree.
All managed thanks to Graham’s teaching skills!
We also checked out Chimera and viewed 3D model images of proteins we had BLASTED-Cytochrome b and opsin ( red and green colour receptor).