JAM Assay for Apoptosis
The Jam assay for quantification of the induction of apoptosis is a novel test developed by Matzinger in 1991 18. It is based on the principle that dying cells often degrade their DNA into small fragments, which are not retained by the filter paper during a typical [3H] Thymidine proliferation assay. The Jam assay measures the DNA retained by living cells rather than the cellular component lost by dying cells. 8,13.
|Buffers and Medias|
Plan Experiment, select and grow effector and target cells. In our system we used as effector cell the choriocarcinoma Jar cell line that express high levels of FasL. As target cells we use the T cell line Jurkat cells which express Fas receptor on its surface. In a 96 well plate; add 200,000 Jar cells in the firs well in 200 µl of normal DMEM media. Prepare serial dilutions of the cells, either 2:1 or 4:1. Incubate overnight.