Madeira, Paul T. and Van, Thai K. and Center, Ted D.
An Improved molecular tool for distinguishing monoecious and dioedious Hydrilla.
Journal of Aquatic Plant Management, 42,
Two biotypes of hydrilla [Hydrilla verticillata(L.f.) Royle]
occur in the United States, a dioecious type centered
in the southeast and a monoecious type in the central Atlantic and northeastern states. Ecosystem managers need tools to distinguish the types as the ranges of each type expand and begin to overlap. A molecular tool using the randomly amplified polymorphic DNA (RAPD) procedure is available but its use is limited by a need for reference samples. We describe an alternative molecular tool which uses “universal primers” to sequence the trnL intron and trnL-F intergenic spacer of the chloroplast genome. This sequence yields three differences between the biotypes (two gaps and one single nucleotide polymorphism). A primer has been designed which ends in a gap that shows up only in the dioecious plant. A polymerase chain reaction (PCR) using this primer produces a product for the monoecious but not the dioecious plant.
||An Improved molecular tool for distinguishing monoecious and dioedious Hydrilla
|Madeira, Paul T.|
|Van, Thai K.|
|Center, Ted D.|
|Journal or Publication Title:
||Journal of Aquatic Plant Management
||Aquatic Plant Management Society, Inc.
||Hydrilla verticillata; trnL intron; trnL-F intergenic
||10 Nov 2008 15:33
||29 Sep 2011 20:14
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