The following experiments are carried out using a pure preparation of the enzyme that catalyzes palmitate synthesis from acetyl CoA and malonyl CoA in the presence of all the cofactors required for reaction.
a) If the acetyl CoA is labeled at the methyl carbon with tritium, and malonyl CoA is unlabeled, how many tritium atoms will be incorporated into each molecule of palmitate formed?
b) If malonyl CoA is supplied in the form of -OOC^3H2COSCoA, and acetyl CoA is unlabeled, how many tritium atoms will be incorporated into each molecule of palmitate formed?
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The solution provides a brief, clear explanation of how to find the number of tritium atoms which will be incorporated in each molecule of the palmitate formed, depending on the condition of the reactants acetyl and maylonyl CoA, including a helpful link to show the reaction more fully.