Research team at Vanderbilt University discover ionising radiation can soften tumour cell microenvironment
Approximately half of all cancer patients receive radiation therapy to reduce the growth of malignant cells. However, little is known about how ionising radiation affects the extracellular matrix (ECM), a patchwork of proteins and other biomolecules that surrounds cells and plays a vital role in their shape, movement and signalling functions. A team of researchers from Vanderbilt University are looking to investigate how irradiation could alter the mechanical properties of the microenvironment.
The team demonstrated that ionising radiation can reduce the rigidity of both the ECM of an extracted tumour and an isolated matrix of collagen fibres. Their results pave the way for irradiation to be used to create matrices with tailored properties and suggest that radiation therapy might have effects beyond disrupting cellular DNA.
“We wanted to know how radiation affects the tissues surrounding cells, particularly how this changes the stiffness of the matrix,” said Prof Cynthia Reinhart-King, an author on the paper. “The change in tissue stiffness during tumour growth can be palpable. Stiffness, for example, is what you would look for in breast self-exams.”
As cancer cells grow and become increasingly dysfunctional, the network of biomolecules surrounding them are deposited more and begin cross-linking further, leading to a thicker, stiffer matrix. This tougher ECM is vital for the cancer cells to travel throughout the body, signal to one another, and form tumours. Targeting the ECM’s stiffness has become a promising tool in attacking cancer cells.
“Twenty years ago, if you had asked what the primary drivers of cancer were and where the primary focus of the research should be, it would have been largely on genetics, and it still is for personalised medicine,” Prof Reinhart-King said.
To date, much of the research on irradiation’s effects on the cellular microenvironment has dealt with samples that included cells, obfuscating the irradiation’s effect on just the ECM rather than the cell’s internal machinery.
Infrared spectroscopy revealed that the radiation does not break down individual fibres of collagen, the long, thin structural proteins that act as the matrix’s scaffolding. Instead, it softens the matrix by cutting the bonds between these fibres, relaxing the matrix and reducing its stiffness.
Prof Reinhart-King’s team discovered that tumour cells were less likely to spread when their stiff ECMs were replaced with ECMs softened by irradiation. Cells suspended in a stiff matrix were more likely to work their way through the matrix to another side of a serum gradient, analogous to how metastasizing cancer cells break free from their tumours. Softened matrices were more likely to hold cells in place.
Prof Reinhart-King said their findings might enable future fractionated radiation — longer term bouts of therapy using a lower intensity of radiation than most of today’s therapies. One particular area of interest is determining if softening can reduce nearby blood vessel leaking and improve drug delivery.
“Since there are risks associated with radiation, we’re interested in investigating how fractionated the dose should be,” Prof Reinhart-King said. “The key will be to find the right balance.”
Source: European Pharmaceutical Review