Saliva sampling is simple and noninvasive, and can be used for extracellular RNA (exRNA) biomarker discovery and molecular diagnosis by utilizing RNA analysis approaches such as RNA-seq. Salivary RNA analysis creates a new paradigm in the field for noninvasive molecular diagnosis. Compared with salivary DNA, salivary RNA is more labile, especially due to the ribonucleases present in saliva. To solve this problem, we have identified some macromolecules that can protect salivary RNA from ribonucleases.
MicroCollect™ Saliva RNA Collection Devices is a cylindrical device with spiral interface funnel. It is convenient to collect saliva by directly spitting into non-toxic buffer preservative solution until the amount of saliva reaches the fill line. The funnel is then removed and the lid is screwed on. Saliva samples can be used in clinical, biochemical and molecular analysis. This device can collect and preserve superior oral samples for molecular detection of RNA, such as RT-PCR, RT-qPCR, RNA sequencing and microarray.
Each MicroCollect™ Saliva RNA Collection Device consists of 3 components: (1) collection funnel and collection tube, (2) preservative, and (3) tube cap. The instructions for use are as follows:
|MicroCollect™ Saliva RNA Collection Devices||50 devices|
|Individual MicroCollect™ Saliva RNA Collection Device Contents|
|Collection funnel and collection tube||1|