Eight polymerase chain reaction primer sets amplifying bi-parentally inherited species-specific markers were developed that differentiate between rainbow trout (Oncorhynchus mykiss) and various cutthroat trout (O. clarki) subspecies. The primers were tested within known F1 and first generation hybrid backcrosses and were shown to amplify codominantly within hybrids. Heterozygous individuals also amplified a slower migrating band that was a heteroduplex, caused by the annealing of polymerase chain reaction products from both species. These primer sets have numerous advantages for native cutthroat trout conservation including statistical genetic analyses of known crosses and simple hybrid identification.
Additional publication details
|Publication Subtype||Journal Article|
|Title||Bi-parentally inherited species-specific markers identify hybridization between rainbow trout and cutthroat trout subspecies|
|Series title||Molecular Ecology Notes|
|Contributing office(s)||Western Fisheries Research Center|
|Online Only (Y/N)||N|
|Additional Online Files (Y/N)||N|