NucleoSpin RNA Plant is designed for isolation and purification of DNA-free, high-quality RNA from a wide variety of plant and fungal samples. The working procedure is similar to the standard RNA purification method without the use of organic compounds (e.g., Trizol)—removal of contaminating DNA is achieved by effective rDNase digestion during the purification process.
The NucleoSpin RNA Plant kit features 2 alternative lysis buffers: in most cases, use of buffer RA1 is recommended for lysis due to its strong denaturing properties. The presence of peculiar metabolites in a variety of plant tissues or fungi, however, may lead to solidification of the lysate, resulting in a non-processible slurry. In such cases, the alternative buffer RAP is the buffer of choice. For thorough homogenization and reduction of lysate viscosity, NucleoSpin Filter Columns are also provided with the kit.