Citrobacter freundii is important causes of nosocomial infections. The present study aimed to evaluate the antibiotic resistance profiles of Citrobacter freundii isolated from the First Bethune Hospital. Disk diffusion method was used to study the antimicrobial resistance. The data were analyzed by WHONET 5 software according to Clinical and Laboratory Standards Institute (CLSI). The majority of 432 strains of Citrobacter freundii were collected from sputum (240, 55.6%), secretions and pus (85, 19.7%), blood (58, 13.4%). All the Citrobacter freundii isolates were sensitive to imipenem and meropenem. Citrobacter freundii strains were frequently resistant to multiple antibiotics. The results suggest that surveillance of antimicrobial resistance in Citrobacter freundii is necessary.