The country’s geophysics service said the eruption of Fuego (Fire) volcano lasted 28 hours.
Ash fell on several nearby villages and towns, reducing visibility at times. Aviation officials cautioned aircraft to steer well clear of the volcano and its ash.
Fuego had previously sent pyroclastic flows of searing debris cascading down its slopes in June and May.
Other than briefly being a hazard to aviation, the recent eruptions have not posed a threat to any nearby inhabited areas.
Guatemala has four active volcanoes that have caused catastrophic damage in the past.
The explosive eruption of Santa Maria in 1902 was one of the world's largest eruptions of the 20th century.
In 2010, a blast at Pacaya volcano coated the current capital, Guatemala City, in a thick layer of ash and rock.
This forced hundreds of families to evacuate and officials to temporarily close the international airport.
Photo: National Coordinator of Disaster Reduction