A student grows a culture of E. coli in 50 ml of liquid medium contained in a 250 ml Erlenmeyer flask and decides to spread plate samples of the culture onto LB agar plates. (LB stands for Luria Broth. Itís simply a type of broth on which E. coli grow well.) Before plating, she sets up several dilutions of the culture. Then she plates 0.1 ml of each final dilution by spread plating. She does this in duplicate for each dilution. She then incubates all of her LB agar plates in the 37 ļC incubator and counts the colonies on each plate 1 day later. Her results are as follows:
this link will show you the tablehttp://s277.photobuc...nt=homework.jpg
a) Assuming that the student carried out the serial dilution steps in the most efficient way possible (i.e. with the least necessary number of dilutions) write out all the steps she performed to get each total dilution factor. Take into consideration the following facts: The final volume at each dilution step was 10 ml and the highest single dilution factor she ever carried out was a 1/100 dilution or 10-2.
Edited by Yousuf, 30 September 2009 - 01:42 PM.