The purpose of the study was to determine factors affecting nurses' attitudes toward computers in healthcare. This cross-sectional study was carried out with nurses employed at one state and one university hospital. The sample of the study included 890 nurses who were selected via a purposive sampling method. Data were collected by using a questionnaire for demographic information and Pretest for Attitudes Toward Computers in Healthcare Assessment Scale v.2. The nurses, in general, had positive attitudes toward computers. Findings of the present study showed a significant difference in attitudes for different categories of age (P < .001), marital status (P < .05), education (P < .001), type of facility (P < .01), job title (P < .001), computer science education (P < .01), computer experience (P < .001), duration of computer use (P < .001), and place of use of computer (P < .001). The results of the present study could be used during planning and implementation of computer training programs for nurses in Turkey and could be utilized in improving the participation of Turkish nurses in initiatives to develop hospital information systems and, above all, in developing computerized patient care planning.