Lartigue, C., Vashee, S., Algire, M. A., Chuang, R. Y., Benders, G. A., Ma, L., Noskov, V. N., Denisova, E. A., Gibson, D. G., Assad-Garcia, N., Alperovich, N., Thomas, D. W., Merryman, C., Hutchison, C. A., 3rd, Smith, H. O., Venter, J. C., Glass, J. I.
Creating Bacterial Strains from Genomes That Have Been Cloned and Engineered In Yeast
Science. 2009 Aug 20;
We recently reported the chemical synthesis, assembly, and cloning of a bacterial genome in yeast. To produce a synthetic cell, the genome must be transferred from yeast to a receptive cytoplasm. Here, we describe methods to accomplish this. We cloned a Mycoplasma mycoides genome as a yeast centromeric plasmid, and then transplanted it into Mycoplama capricolum to produce a viable M. mycoides cell. While in yeast, the genome was altered using yeast genetic systems, and then transplanted to produce a new strain of M. mycoides. These methods allow the construction of strains that could not be produced with genetic tools available for this bacterium.