Researchers have developed an add-on for laser scanning microscopes that can improve the quality of 2D and 3D images of the brain, according to a new study published in Optica .
The addition, called PySight, includes both hardware and open source software. A laser-based image technique called multiphoton microscopy is often used to capture high quality 2D and 3D images of neurons, blood vessels and other parts of the brain of a patient, the writers observed , but it can be difficult because the images need to be taken quickly. . This causes fewer photons to be visible in the final image.
"To overcome this obstacle, microscopes used a detector readout method called photon counting," said research team leader Pablo Blinder of Tel Aviv University in Israel. "Because the implementation required extensive electronic knowledge and personal components, photon counting has never been widely accepted. In addition, commercially available solutions were inappropriate to perform very fast images as needed for 3D imaging."