PowerPoint Slideshow about 'Glucose-6-Phosphate Dehydrogenase (G6PD)' - JasminFlorian
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is the most common human enzyme deficiency in the world; it affects an estimated 400 million people. G6PD deficiency is also known as "favism," since G6PD deficient individuals are also sometimes allergic to fava beans. G6PD deficiency is an allelic abnormality which is inherited in an X-linked recessive fashion
When someone has G6PD deficiency, complications can arise; hemolytic anemia and prolonged neonatal jaundice are the two major pathologies associated with G6PD deficiency. Both of these conditions are directly related to the inability of specific cell types to regenerate reduced nicotinamide adenine dinucleotide phosphate (NADPH); this reaction is normally catalyzed by the G6PD enzyme.
Glucose-6-phosphate dehydrogenase (G6PDH, D-glucose-6-phosphate) catalyzas the first step in the pentose phosphate shunt ,oxidising glucose-6-phosphate (G-6-P)to 6-phosphogluconate(6-PG) and reducing NADP to NADPH.
G-6-P + NADP+ 6-PG + NADPH + H+
NADP is reduced by G-6-PDH in the presence of G-6-P. The rate of formation of NADPH is directly proportional to the G-6-PDH activity and is measured spectrophotometrically as an increased in absorbance at 340nm. Prodution of asecond molar equivalant of NADPH by erythrocyte 6-phosphogluconate dehydrogenase (6-PGDH) according to the reaction :
Glucose -6-phosphate dehydrogenase,present in the red blood cell hemoysate, act on glucose -6-phosphate and reduces NADP to NADPH which, with the help of PMS, reduces blue colored 2,6Dichlorophenol Indophenol into acolorless form.the rate of decolorization is proportional to the enzynme activity. The reaction can be represented as: