Immunofluorescent images of formaldehyde-fixed cell lines are shown. Three different organelle markers are displayed as different channels in the multicolor images - nucleus stained in blue, microtubules in red and ER in yellow. The various cell structures that are demonstrated are always shown in the green channel using an antibody found in the Human Protein Atlas. The antibody id is linked to the corresponding Cell Atlas protein page. By using the "toggle channels"-buttons, the different channels can be turned on and off. Most cell structures can be highlighted in the cell illustration by hovering over them with the exception of the aggresome. Cytoplasmic bodies are highlighted as cytosol; cytokinetic bridge, midbody, midbody ring and mitotic spindle are highlighted as microtubules, cell junctions are highlighted as plasma membrane and nucleus is highlighted as nucleoplasm.
Staining of actin filaments in human cell line U-2 OS (HPA070701)
Scale bar represents 10µm
Actin filaments are assembled close to the plasma membrane, either as long, fragile bundles of filaments, or small patches. They make up part of the cell cortex, a supportive layer just beneath the plasma membrane, and are responsible for both cell movement and morphology. Actin filaments end with focal adhesions, which connect the cell to the extracellular matrix.
Actin filaments are microfilaments, which make up part of the cellular cytoskeleton. They assemble either as long bundles of filaments or as small patches and make up a part of the cell cortex, a supportive layer just beneath the plasma membrane. In this role they directly interact with focal adhesions, which connect the cell to the extracellular matrix. Together they are responsible for controlling both cell movement and morphology. Actin filaments can also serve as avenues for intracellular vesicle transport and make up the midbody ring, which is essential for cytokinesis.