Phycology media can be prepared from concentrated solutions or from powdered salt mixtures. The concentrated solutions are complete, including vitamins, and should be stored frozen. The powdered media allow flexibiltiy in customizing the final media composition. The powders to prepare growth media for aquatic species consist of "enrichment" salt mixtures and salt mixtures for completely synthetic media. The powders or concentrated solutions are added to freshwater or seawater to prepare enriched freshwater or enriched marine media, respectively. To prepare synthetic marine growth media the powder is added to tissue culture grade freshwater. Published formulas of growth media for aquatic species often include sodium bicarbonate. Sodium bicarbonate is not included in our powdered mixtures and should be added during medium preparation, if required for your application.
Powdered media are extremely hygroscopic and must be protected from atmospheric moisture. If possible the entire contents of each package should be used immediately after opening. Preparing the medium in a concentrated form is not recommended as some salt complexes may precipitate. Supplements that are added to the medium may affect shelf life and storage conditions. The basic steps for preparing the culture medium are listed below:
POWDERED MEDIA AND BASAL SALT MIXTURES ARE FOR LABORATORY USE ONLY. NOT FOR DRUG, HOUSEHOLD OR OTHER USES.
Materials Not Provided
Auxins, cytokinins, carbohydrates, gelling agents, and other supplements listed in the Biochemicals section.
Store dry medium in a desiccator at 0-5 °C. Deterioration of powdered medium may be recognized by: 1) color change; 2) granulation, clumping, or particulate matter throughout the powder; 3) pH change; or 4) inability to promote growth when properly used.
Precipitation in Media
Precipitates are known to occur, with time, in plant tissue culture media. The precipitates have been analyzed (unpublished internal data; Dalton, et al. 1983). They are composed of small, pale yellow-white particles. Analysis of precipitates indicated a predominance of iron, phosphate, and zinc. The probable cause of the precipitates is the inevitable oxidation of ferrous ions to ferric ions and the presence of unchelated ferric ions. When the solubility of ferric phosphate is exceeded precipitation occurs. There are no reports of detrimental effects on growth and development in plant tissue culture due to the precipitates.
B5282 – Bold modified basal freshwater nutrient solution
C3061 – Cyanobacteria BG-11 freshwater solution (50X)
G0154 – Guillard's (f/2) marine water enrichment solution
G9903 – Guillard's (f/2) marine water enrichment solution