It appears that your browser may be outdated and performance may be limited. The My Study builder is best viewed with the latest internet browsers. However, if you’d prefer to use our standard form, you can access it below.
Interleukin-1β (IL-1 beta) is one of a family of biologically active small protein molecules known as cytokines. Cytokines are produced by a number of different cell types, including macrophages, monocytes, fibroblasts, and dendritic cells. (1,2,3) IL-1β is an example of a pro-inflammatory cytokine, since it is involved in the body’s inflammatory response to acute or chronic infections, or to conditions that are associated with a persistent low-grade inflammatory state, such as obesity. (4,5) IL-1β is therefore frequently used as a bio-marker of inflammation. (6,7) A study with normal mouse parotid acinar cells has shown that they synthesize IL-1β and store it in secretory granules. IL-1β is released from the granules following α- and β-adrenergic stimulation. (8) Relationships between IL-1β levels in blood and saliva are not fully understood.
*Add 300 µl to the total volume of all tests for liquid handling
IL-1 Beta may be Flow Rate Dependent
3.13 pg/mL - 200 pg/mL
0.05 - 2256 pg/mL
Collect Saliva Samples
IL-1 BETA SALIVA COLLECTION CONSIDERATIONS
Better results begin with better saliva collection. This collection protocol features general considerations to maximize salivary IL-1 Beta analysis. Use this analyte specific collection protocol to plan your collection methodology and sampling schemes.
Considerations for adding Salivary DNA to analyte Studies:
You can combine salivary analytes with easy, accurate, and affordable genomic testing using Salimetrics SalivaLab and the same sample that you are already collecting – no specialized saliva collection devices or additional samples are required.
Don’t know what SNPs are right for you? The SalivaLab’s DNA team specializes in genetic testing services, we recommend you Request a DNA Consult (gratis) to learn more about common considerations such as # of samples, participant ethnicity, and IRB Approval.
Giannobile, W.V., Beikler, T., Kinney, J.S., et al. (2009). Saliva as a diagnostic tool for periodontal disease: Current state and future directions. Periodontology 2000, 50, 52-64.
Pirhonen, J., Sareneva, R., Kurimoto, M., et al. (1999). Virus infection activates IL-1β and IL-18 production in human macrophages by a caspase-1-dependent pathway. J Immunol, 162(12), 7322-29.
Park, D.R., Thomsen, A.R., Frevert, C.W. et al. (2003). Fas (CD95) induces proinflammatory cytokine responses by human monocytes and monocyte-derived macrophages. J Immunol, 170(12), 6209-16.
Hernández-Rodríguez, J., Segarra, M., Vilardell, C., et al. (2004). Tissue production of pro-inflammatory cytokines (IL-1β, TNFα, and IL-6) correlates with the intensity of the systemic inflammatory response and with corticosteroid requirements in giant-cell arteritis. Rheumatology (Oxf.), 43(3), 294-301.
Southerland, J.H., Taylor, G.W., Moss, K., et al. (2006). Commonality in chronic inflammatory diseases: Periodontitis, diabetes, and coronary artery disease. Periodontology 2000, 40, 130-43.
Tzouvelekis, A., Pneumatikos, I, Bouros, D. (2005). Serum biomarkers in acute respiratory distress syndrome an ailing prognosticator. Respir Res, 6, 62.
Miller, C.S., King C.P., Langub, M.C., et al. (2006). Salivary biomarkers of existing periodontal disease: A cross-sectional study. J Am Dent Assoc, 137(3), 322-29.
Tanda, N., Ohyama, H., Yamakawa, M., et al. (1998). IL-1β and IL-6 in mouse parotid acinar cells: Characterization of synthesis, storage, and release. Am J Physiol, 274(1 Pt1), G147-56.
Safe Saliva Collection in the COVID-19 Era
We have Good News!
Successfully recalibrate your saliva collection and handling procedures with these new safety and compliance guidelines for the COVID-19 era. These guidelines are good practice, easy to follow, and will allow you to begin or continue your research during this historical period of stress and unease… (Read More)