The aim of this work was the identification of mastitic streptococci by cultivation and biochemical tests, and, where necessary, by serological methods. It was concluded that horse-serum agar was an excellent medium for the isolation of mastitic streptococci. Addition of 0. 1% aesculine improved diagnosis and allowed most kinds of mastitic streptococci to be recognized immediately. A simple guide elucidates the practical use of the cultural medium. Main criteria were behaviour with aesculine and litmus milk, type of colonies, haemolytic or not, and behaviour with raffinose and trehalose.
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