Meets AP Science Practices 1, 2, 4, 5, 6, and 7
- Addresses Big Ideas 1 & 6
- Materials provided for 30 students working in pairs
- Investigation includes updated literature aligned with new AP Chemistry curriculum standards
In this lab, students will explore how components in a buffer solution affect the solution's buffering capacity. Students will prepare a number of different buffer solutions, and will determine the response of these buffers as acids and bases are added. The pH of these stressed buffers will then be compared with the calculated values from the Henderson-Hasselbalch equation. From the buffer solutions prepared, a buffer system will be proposed in order to address a specific pH buffering microbiology problem. Includes acetic acid, ammonia, sodium acetate, ammonium chloride, sodium hydroxide, hydrochloric acid, sodium dihydrogen phosphate, and sodium hydrogen phosphate. Requires pH meter, magnetic stirrer, magnetic stir rod, analytical balance, burette, and ring stand/clamps, available separately. Ward's® DataHub not required to complete activity.
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Sodium Dihydrogen Citrate
Sodium Dihydrogen Phosphate
Sodium Hydrogen Phosphate
Required but Not Provided: Beakers, Stirring Rods/Magnetic Stirrer, pH Meter, Analytical Balance, DI Water, Graduated Cylinders, Burettes/Clamps, Ring Stands, Volumetric Flasks, Alka Seltzer (optional), pH paper (optional), Gloves, Safety Goggles, Lab Aprons.