One thing people usually know that human red blood cells do not have cell nuclei, so they are lacking chromosomal DNA. But far less people have a guess about mitochondria’s presence in the erythrocytes. So let’s ask the experts Wikipedia. The answer is NO, mammalian red blood cells also lose their mitochondria during erythropoiesis at phase 3, when normoblasts eject organelles. Functional red blood cells produce energy by fermentation, via anaerobic glycolysis of glucose followed by lactic acid production. As the cells do not own any protein coding DNA they cannot produce new structural or repair proteins or enzymes and their lifespan is limited.