The development of techniques and methods for protein purification was an essential pre-requisite for many modern biotechnological advancements. Protein purification varies from simple one-step precipitation procedures to large-scale validated production processes. However, in many cases, more than one purification step is required to achieve the desired purity. The key to successful and efficient protein purification is to select the most appropriate techniques, optimise their performance to suit the experimental requirements, and combine them in a logical way to maximise yield and minimise the number of steps required.
Most purification schemes involve some form of chromatography. As a result, chromatography has become an essential tool in every laboratory in which protein purification is needed. The availability of different chromatography techniques with different levels of selectivity means that powerful methodological combinations are now available for the purification of any biomolecule. Proteins can even be produced in forms which facilitate their subsequent chromatographic purification.
However, this ability has not removed every challenge. Host contaminants may still be present and problems related to solubility, structural integrity, and biological activity could still exist. Nonetheless, although it may appear that there are a huge number of parameters to consider, the process can be planned and performed simply and easily, with only a basic knowledge of the details of chromatography techniques.
- Equipment and Resources
- An AKTA FPLC for general protein purification.
- An ETTAN LC for small-scale protein purification.
- Protein purification.
Contact the protein purification team:
963289680 ext. 5312