cDNA Library Construction, Normalization and Screening
From hundreds of thousands of protein molecules, picking up one that is related to a particular biological phenomenon is a challenging task. To help researchers accomplish this task, we have developed proprietary technologies to construct cDNA libraries especially for this purpose. The cDNA libraries we constructed have an extraordinary complexity, a high percentage of full length clones, and high expression efficiency. With such libraries plus our systematic screening techniques and experience, we can help researchers pick up the cDNA clones which encode the proteins they are looking for.
- Construction of cDNA libraries from tissues, cells (1 million), total RNA (10 mg), or mRNA (200 ng). cDNA is directionally cloned into an expression plasmid without being digested by restriction enzymes. Our standard libraries contain more than 3 million primary clones and no PCR is used during construction to avoid PCR related artifacts. Services for construction of large insert cDNA libraries are available.
- Normalization of cDNA libraries. The normalization procedure will reduce the abundance of the cDNA clones of the house-keeping genes 10 to 200 fold.
- cDNA library screening. We use functional screening to identify the cDNA clones, which are related to particular biological phenomena from cDNA libraries.