The quality of the water is a universal necessity that demands attention of sanitary authorities and general consumers, mainly for fountains like wells, springs and headwaters destined to human consumption, since their contamination by human and animal excretions may become a vehicle that transmit diseases. The objective of this research was to evaluate the microbiological conditions and the potability of water in three headwaters located in S o Francisco’s slum in Campo Mour o - Paraná. The multiple tube method was used to microbiological evaluation of water. Presumptive and confirmative test for total coliform bacteria and the test for presence of thermotolerant coliforms were carried out. The results have shown that water collected in three evaluated headwater were not up to the microbiological potability standards determined by the National Agency of Health Surveillance. The families which use this water are in risky of contracting diseases. It demonstrates the importance of alert the competent authorities about the necessity of drinking water supply and garbage disposal in S o Francisco slum.