Rotavirus is a gastrointestinal disease that causes severe diarrhea, vomiting, fever, and weight loss. It occurs most often in infants and young children and can lead to severe and dangerous dehydration. Children in developing regions are most at risk for rotavirus infection. The infection spreads via the fecal-oral route.
The IVD Rotavirus Stool Antigen Detection Microwell ELISA test kit provides accurate, reliable detection of Rotavirus antigens in human stool samples. Enzyme-linked immunosorbent assays (ELISA) using stool samples have become the preferred clinical method for diagnosing rotavirus infection, providing both rapid and cost-effective detection of rotavirus antigens.
Read more about how rotavirus is transmitted, diagnosed, and treated on our Articles page.
The IVD Rotavirus Stool Antigen Detection Microwell ELISA test is performed in five steps (four incubation periods and a stop solution):
- The first incubation: In this step, antibodies attached to the kit microwells capture rotavirus-specific antigens present in the stool sample; a washing step removes any unbound samples.
- The second incubation: This step adds a second anti-rotavirus antibody to “sandwich” the antigens; a washing step removes any unbound conjugate.
- The third incubation: This step adds antibodies conjugated to horseradish peroxidase to the “sandwich” from the previous step; a washing step removes any unbound conjugate.
- The fourth incubation: This step adds a chromogen, which turns blue in the presence of the enzyme complex and peroxide.
- The addition of a stop solution: This ends the chemical reaction and turns the blue to yellow in the presence of adenovirus antigen. If no antigen is captured, or if there is an insufficient antigen level, the solution will remain colorless.
Each test kit contains 96 wells and has a shelf life of up to 12 months. Test kits should be stored between 2-8 ˚C. Please contact us for pricing and availability.