January 30, 2007
CONTACT: Karen Kreeger
Molecular Motors and Brakes Work
Together in Cells
(PHILADELPHIA) – Researchers at the University
of Pennsylvania School of Medicine have discovered that microtubules
– components responsible for shape, movement, and replication within
cells – use proteins
that act as molecular motors and brakes to organize into their correct
structure. If microtubules are not formed properly such basic functions
division and transport can go wrong, which may have implications in
such disease processes as cancer
The study, published in the January issue of Cell,
is featured on the cover of that issue.
In the Cell study, the investigators, working with fission yeast cells, showed that stable end-to-end arrays of microtubules can be achieved by a balance between the sliding by a molecular motor called klp2p and the braking of a microtubule-associated protein (MAP) called ase1p. Specifically, they showed that a preexisting “mother” microtubule acts as a platform on which a new microtubule can be formed (view video here). The new “daughter” microtubule grows and moves along the mother microtubule. In time, the daughter grows beyond the end of the mother to ultimately produce two microtubules, connected by the cross-linking MAP ase1p.
“Imagine that the daughter microtubule is a short train on the track of the mother microtubule,” explains Tran. “The molecular motor is the train’s engine, but the problem is that the cargo – the molecular brakes – gets longer, slowing down the daughter train. But when the train gets to the end of the track it remains attached to the end of mother microtubule. At the tail end, it stops moving and that defines the region of overlap. Our work shows that the cell can make microtubule structures of defined lengths stable by coordinating the sliding of the motors and the slowing of the brakes.”
If microtubule-based structures are not formed properly because of failures in brakes or motors, such basic functions as cell division and cell transport can go awry, with such diseases as cancer and dementia possibly resulting. “For the first time we have shown how MAPs and motors work together in a mechanistic way,” says Tran. “This is important and it will make other people who study microtubules rethink how they study the cell.”
Co-authors are Marcel E. Jansen, Isabelle Loïodice, and Chuanhai Fu, all from Penn, and Rose Loughlin, Damian Brunner, and François J. Nédélec from the European Molecular Biology Laboratory, Heidelberg, Germany. The research was sponsored by the National Institutes of Health.
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