Acid-fast bacteria, such as Mycobacteria, are resistant to decolorization by acids during staining procedures. The high mycolic acid content of Mycobacteria, is responsible for the staining pattern of poor absorption followed by high retention. The most common staining technique used to identify acid-fast bacteria is the Ziehl-Neelsen stain or acid-fast stain, in which the acid-fast bacilli are stained bright-red and stand out clearly against a blue background. L-form bacteria are strains of bacteria that lack cell walls. The main pathogenic bacteria in this class is Mycoplasma (not to be confused with Mycobacteria).
What is the most typical staining technique used to identify acid-fast bacteria?
Ziehl-Neelsen stain