F4/80 in Mouse Lung. F4/80 was detected in immersion fixed frozen sections of mouse lung using Rat Anti-Mouse F4/80 Monoclonal Antibody  at 15 ug/mL overnight at 4 ﾰC. Tissue was stained using the NorthernLightsﾙ 557-conjugated Anti-Rat IgG Secondary Antibody (red