Detection of persisters in Candida albicans biofilms. ( a ) Survival of Candida albicans cells in biofilms of ATTC 90028 and C1 strain following treatment with AmphB at different concentrations. CLSM images of C1 biofilm before ( b ) and after ( c ) treatment with 100 µg mL −1 AmphB. In the untreated biofilm cells appear green (live), in the treated biofilm the majority of cells are red (dead). Arrows indicate the few persisters. 