 Figure 4. 1% Agarose gel for yH4 mutant. After purifying the mutant plasmid, the plasmid was run on a gel with a standard to verify the presence of DNA and its concentration. Two yH4 plasmids were mutated in the experiment; A, B, and C represent one copy at different volumes, while A1, B1, C1 represent the other copy. The first copy was later the one chosen to be used to make the octamer. The standard (-306 HTLV promoter DNA) was added in different concentrations: 5, 10, 20, 50, and 100 ng. It was concluded that the yH4 plasmid DNA was about 100 ng/ul by comparing A' with the standard bands of DNA. 