  Counting circular objects such as cell colonies is an important source of
information for biologists. Although this task is often time-consuming and
subjective, it is still predominantly performed manually. The aim of the
present work is to provide a new tool to enumerate circular objects from
digital pictures and video streams. Here, I demonstrate that the created
program, OpenCFU, is very robust, accurate and fast. In addition, it provides
control over the processing parameters and is implemented in an in- tuitive and
modern interface. OpenCFU is a cross-platform and open-source software freely
available at http://opencfu.sourceforge.net.
