  A targeted imaging system has been developed for applications requiring
recording from stationary samples at high spatiotemporal resolutions. It works
by illuminating regions of interest in rapid sequence, and recording the signal
from the whole field of view onto a single photodetector, and can be
implemented at low cost on an existing microscope without compromising existing
functionality. The system is characterized in terms of speed, spatial
resolution, and tissue penetration depth, before being used to record
individual action potentials from ASAP-3 expressing neurons in an ex vivo mouse
brain slice preparation.
