  ExpressInHost (https://gitlab.com/a.raguin/expressinhost) is a GTK/C++ based
user friendly graphical interface that allows tuning the codon sequence of an
mRNA for recombinant protein expression in a host microorganism. Heterologous
gene expression is widely implemented in biotechnology companies and academic
research laboratories. However, expression of recombinant proteins can be
challenging. On the one hand, maximising translation speed is important,
especially in scalable production processes relevant to biotechnology
companies, but on the other hand, solubility problems often arise as a
consequence, since translation "pauses" might be key to allow the nascent
polypeptide chain to fold appropriately. To address this challenge, we have
developed a software that offers three distinct modes to tune codon sequences
using the genetic code redundancy. The tuning strategies implemented take into
account the specific tRNA resources of the host and that of the native
organism. They balance rapid translation and native speed mimicking, which
might be important to allow proper protein folding, thereby avoiding protein
solubility problems.
