  The in vitro and in vivo activity of diminazene (Dim), artesunate (Art) and
combination of Dim and Art (Dim-Art) against Leishmania donovani was compared
to reference drug; amphotericin B. IC50 of Dim-Art was found to be $2.28 \pm
0.24 \mu$ g/mL while those of Dim and Art were $9.16 \pm 0.3 \mu$ g/mL and
$4.64 \pm 0.48 \mu$ g/mL respectively. The IC50 for Amphot B was $0.16 \pm 0.32
\mu$ g/mL against stationary-phase promastigotes. In vivo evaluation in the L.
donovani BALB/c mice model indicated that treatments with the combined drug
therapy at doses of 12.5 mg/kg for 28 consecutive days significantly ($p <
0.001$) reduced parasite burden in the spleen as compared to the single drug
treatments given at the same dosages. Although parasite burden was slightly
lower ($p < 0.05$) in the Amphot B group than in the Dim-Art treatment group,
the present study demonstrates the positive advantage and the potential use of
the combined therapy of Dim-Art over the constituent drugs, Dim or Art when
used alone. Further evaluation is recommended to determine the most efficacious
combination ratio of the two compounds.
