  Construction of in vitro vascular models is of great significance to various
biomedical research, such as pharmacokinetics and hemodynamics, thus is an
important direction in tissue engineering. In this work, a standing surface
acoustic wave field was constructed to spatially arrange suspended endothelial
cells into a designated patterning. The cell patterning was maintained after
the acoustic field was withdrawn by the solidified hydrogel. Then, interstitial
flow was provided to activate vessel tube formation. Thus, a functional
vessel-on-a-chip was engineered with specific vessel geometry. Vascular
function, including perfusability and vascular barrier function, was
characterized by beads loading and dextran diffusion, respectively. A
computational atomistic simulation model was proposed to illustrate how solutes
cross vascular lipid bilayer. The reported acoustofluidic methodology is
capable of facile and reproducible fabrication of functional vessel network
with specific geometry. It is promising to facilitate the development of both
fundamental research and regenerative therapy.
