  The spatial localization or sequestering of motile cargo and their dispersal
within cells is an important process in a number of physiological contexts. The
morphology of the cytoskeletal network, along which active, motor-driven
intracellular transport takes place, plays a critical role in regulating such
transport phases. Here, we use a computational model to address the existence
and sensitivity of dynamic sequestering and how it depends on the parameters
governing the cytoskeletal network geometry, with a focus on filament lengths
and polarization away or toward the periphery. Our model of intracellular
transport solves for the time evolution of a probability distribution of cargo
that is transported by passive diffusion in the bulk cytoplasm and driven by
motors on explicitly rendered, polar cytoskeletal filaments with random
orientations. We show that depending on the lengths and polarizations of
filaments in the network, dynamic sequestering regions can form in different
regions of the cell. Furthermore, we find that, for certain parameters, the
residence time of cargo is non-monotonic with increasing filament length,
indicating an optimal regime for dynamic sequestration that is potentially
tunable via filament length. Our results are consistent with {\it in vivo}
observations and suggest that the ability to tunably control cargo
sequestration via cytoskeletal network regulation could provide a general
mechanism to regulate intracellular transport phases.
