  Accurately measuring cell stiffness is challenging due to the invasiveness of
traditional methods like atomic force microscopy (AFM) and optical stretching.
We introduce a non-invasive off-axis system using holographic imaging and
acoustic stimulation. This system features an off-axis Mach-Zehnder
interferometer and bulk acoustic waves to capture cell mechanics. It employs
high-resolution components to create detailed interferograms and allows
continuous imaging of cell deformation. Unlike conventional techniques, our
method provides high-throughput, label-free measurements while preserving cell
integrity. Polyacrylamide beads are tested for high precision, highlighting the
potential of the system in early cancer detection, disease monitoring, and
mechanobiological research.
