  Our growing ability to tailor healthcare to the needs of individuals has the
potential to transform clinical treatment. However, the measurement of multiple
biomarkers to inform clinical decisions requires rapid, effective, and
affordable diagnostics. Chronic diseases and rapidly evolving pathogens in a
larger population have also escalated the need for improved diagnostic
capabilities. Current chemical diagnostics are often performed in centralised
facilities and are still dependent on multiple steps, molecular labelling, and
detailed analysis causing the result turnaround time to be over hours and days.
Rapid diagnostic kits based on lateral flow devices can return results quickly
but are only capable of detecting a handful of pathogens or markers. Herein we
present the use of disposable plasmonics as a platform for low-cost label-free
optical biosensing with multiplexing capabilities, eliminating the need for
flow systems often required in current optical biosensors. We demonstrate the
biosensing capabilities of a system that uses chiroptical measurements as well
as plasmonic enhanced fluorescence from metafilms with shuriken shaped
nanoindentations. We also perform the detection of SARS-CoV-2 in complex
solutions as an early developmental milestone towards high-throughput,
single-step, diagnostic kits for differential diagnosis of multiple respiratory
viruses and any other emerging diagnostic needs. Diagnostics based on this
platform which we term Disposable Plasmonics Assays, would be suitable for
low-cost screening of multiple pathogens or biomarkers in a near point of care
setting.
