  Multiview light sheet fluorescence microscopy (LSFM) allows to image
developing organisms in 3D at unprecedented temporal resolution over long
periods of time. The resulting massive amounts of raw image data requires
extensive processing interactively via dedicated graphical user interface (GUI)
applications. The consecutive processing steps can be easily automated and the
individual time points can be processed independently, which lends itself to
trivial parallelization on a high performance cluster (HPC). Here we introduce
an automated workflow for processing large multiview, multi-channel,
multi-illumination time-lapse LSFM data on a single workstation or in parallel
on a HPC. The pipeline relies on snakemake to resolve dependencies among
consecutive processing steps and can be easily adapted to any cluster
environment for processing LSFM data in a fraction of the time required to
collect it.
