  Metabolite analysis of peritoneal dialysis (PD) effluent may provide
information regarding onset and progression of complications associated with
prolonged PD therapy. In this context, the NMR detectable small metabolites of
PD effluent samples were characterized using high resolution 1H and 1H-13C NMR
spectroscopy. The various spectra were recorded (at 800 MHz proton frequency)
on PD effluent samples obtained after 4 hour (intraperitoneal) dwell time from
patients with end stage renal failure (ESRF) and continuing normally on PD
therapy. Inspite of devastating spectral feature of PD effluent due to the
presence of intense resonances from glucose and lactate, we were able to
identify about 53 small endogenous metabolites (including many complex coupled
spin systems) and more than 90 % of the total CH cross peaks of 1H-13C HSQC
spectrum were identified specific to various metabolites of PD effluent. We
foresee that the characteristic fingerprints of various metabolites of control
PD effluent samples will be used to identify and distinguish metabolic
differences from PD related complications.
