  We study the ADHM construction of instantons in N=2 supersymmetric Yang-Mills
theory deformed in constant Ramond-Ramond (R-R) 3-form field strength
background in type IIB superstrings. We compare the deformed instanton
effective action with the effective action of fractional D3/D(-1) branes at the
orbifold singularity of C^2/Z_2 in the same R-R background. We find discrepancy
between them at the second order in deformation parameters, which comes from
the coupling of the translational zero modes of the D(-1)-branes to the R-R
background. We improve the deformed action by adding a term with space-time
dependent gauge coupling. Although the space-time action differs from the
action in the omega-background, both actions lead to the same instanton
equations of motion at the lowest order in gauge coupling.
