  CBCT-guided small animal irradiators encounter challenges in localizing
soft-tissue targets due to low imaging contrast. Bioluminescence tomography
(BLT) offers a promising solution, but they have largely remained in
laboratorial development, limiting accessibility for researchers. In this work,
we develop a universal, commercial-graded BLT-guided system (MuriGlo) designed
to seamlessly integrate with commercial irradiators and empower researchers for
translational studies. We demonstrate its capabilities in supporting in vitro
and in vivo studies. The MuriGlo comprises detachable mouse bed, thermostatic
control, mirrors, filters, and CCD, enabling multi-projection and
multi-spectral imaging. We evaluate that the thermostatic control effectively
sustains animal temperature at 37{\deg}C throughout imaging, and quantify that
the system can detect as few as 61 GL261-AkaLuc cells in vitro. To illustrate
how the MuriGlo can be utilized for in vivo image-guided research, we present 3
strategies, BLT-guided 5-arc, 2-field box, and BLI-guided single-beam, ranging
from complicated high-conformal to simplest high-throughput plans. The high
conformal BLT-guided 5-arc plan fully covers the gross tumor volume (GTV) at
prescribed dose with minimal normal tissue exposure (3.9%), while the
simplified, high-throughput BLT-guided 2-field box achieves 100% GTV coverage
but results in higher normal tissue exposure (13.1%). Moreover, we demonstrate
that the localization accuracy of MuriGlo for both widely-used SARRP and SmART
irradiators is within1 mm, and the tumor coverage reaches over 97% with 0.75mm
margin. The universal BLT-guided system offers seamless integration with
commercial irradiators, achieving comparable localization accuracy, expected to
supporting high-precision radiation research.
