  The photosystem II reaction center (PSII-RC) performs the primary energy
conversion steps of oxygenic photosynthesis. While the PSII-RC has been studied
extensively, the similar timescales of energy transfer and charge separation,
and the severely overlapping pigment transitions in the Qy region have led to
multiple models of its charge separation mechanism and excitonic structure.
Here we combine two-dimensional electronic spectroscopy (2DES) with a continuum
probe and two-dimensional electronic vibrational spectroscopy (2DEV) to study
the cyt b559-D1D2 PSII-RC at 77K. This multispectral combination correlates the
overlapping Qy excitons with distinct anion and pigment-specific Qx and mid-IR
transitions to resolve the charge separation mechanism and excitonic structure.
Through extensive simultaneous analysis of the multispectral 2D data we find
that charge separation proceeds on multiple timescales from a highly
delocalized excited state via a single pathway in which PheoD1 is the primary
electron acceptor, while ChlD1 and PD1 act in concert as the primary electron
donor.
