  Once the magnesium alloy was implanted in the body, it was immediately
covered with plasma proteins. The coated alloy surface promoted the adsorption
and growth of osteoblasts. Herein, in vitro biological reactions of the ZK60
and AZ31 magnesium alloys were analyzed with and without plasma proteins
incubation. The plasma proteins adsorbed on the magnesium alloy were
characterized using mass spectrometry (MS). The MS results show that proteins
related to bone cells such as fibrinogen, vitronectin, fibronectin, and
prothrombin are prone to adsorbed on the surface of the alloys than other
proteins. These proteins restrain the degradation of Mg alloys and promote the
adsorption and growth of bone cells, which demonstrated by the immersion tests
and biocompatibility assays. Furthermore, molecular dynamics simulations were
used to analyze the details of the adsorptions of fibrinogen, fibronectin, and
prothrombin on ZK60 and AZ31at atomic level. It is revealed that the type of
residues adsorbed on the surface of the material has an important effect on
protein adsorption.
