  The use of polymer-based surfactants in the double-emulsion (water/oil/water,
W/O/W) solvent-evaporation technique is becoming a widespread strategy for
preparing biocompatible and biodegradable polymeric nanoparticles (NPs) loaded
with biomolecules of interest in biomedicine, or biotechnology. This approach
enhances the stability of the NPs, reduces their size and recognition by the
mononuclear phagocytic system, and protects the encapsulated biomolecule
against losing biological activity. Different protocols to add the surfactant
during the synthesis lead to different NP colloidal properties and biological
activity.
