  Coral reef science is a fast-growing field propelled by the need to better
understand coral health and resilience to devise strategies to slow reef loss
resulting from environmental stresses. Key to coral resilience are the
symbiotic interactions established within a complex holobiont, i.e. the
multipartite assemblages comprising the host coral organism, endosymbiotic
dinoflagellates, bacteria, archaea, fungi, and viruses. Tara Pacific is an
ambitious project built upon the experience of previous Tara Oceans
expeditions, and leveraging state-of-the-art sequencing technologies and
analyses to dissect the biodiversity and biocomplexity of the coral holobiont
screened across most archipelagos spread throughout the entire Pacific Ocean.
Here we detail the Tara Pacific workflow for multi-omics data generation, from
sample handling to nucleotide sequence data generation and deposition. This
unique multidimensional framework also includes a large amount of concomitant
metadata collected side-by-side that provide new assessments of coral reef
biodiversity including micro-biodiversity and shape future investigations of
coral reef dynamics and their fate in the Anthropocene.
