  Structured illumination microscopy (SIM) achieves doubled spatial resolution
by exciting the specimen with a high-contrast, high-frequency sinusoidal
pattern. Such an excitation pattern can be generated by interference between
multiple laser beams, which are diffracted from a grating. In SIM, 2D imaging
requires 9 patterns and 3D imaging requires 15 patterns. Compared to mechanical
movement of gratings, opti-electro devices provide rapid switch of the
excitation patterns, in which Digital Micro-mirror Device (DMD) is most common
in industry. Here we model DMD as the blazed grating and report a fast and
cost-efficient SIM. Our home-built DMD-based laser interference structured
illumination microscopy (DMD-ISIM) system reveals the nuclear pore complex and
microtubule in mammalian cells with doubled spatial resolution. We further
proposed multi-color DMD-ISIM system with simulation, which could potentially
exploit the full power of DMD-ISIM.
