  We present a comprehensive guide to light-sheet microscopy (LSM) to assist
scientists in navigating the practical implementation of this microscopy
technique. Emphasizing the applicability of LSM to image both static microscale
and nanoscale features, as well as diffusion dynamics, we present the
fundamental concepts of microscopy, progressing through beam profile
considerations, to image reconstruction. We outline key practical decisions in
constructing a home-built system and provide insight into the alignment and
calibration processes. We briefly discuss the conditions necessary for
constructing a continuous 3D image and introduce our home-built code for data
analysis. By providing this guide, we aim to alleviate the challenges
associated with designing and constructing LSM systems and offer scientists new
to LSM a valuable resource in navigating this complex field.
