  Atherosclerotic disease develops when there is endothelial dysfunction,
making low density lipoproteins (LDL) which are cholesterol carriers traveling
in the blood, very likely to penetrate the endothelium layer triggering the
formation of the atheromatous plaque and leading to the obstruction of blood
vessels. For some time now, devices called stents have been used to re-open the
light of the plugged artery, allowing the restoration of blood flow. However,
this treatment has disadvantages like developing intra-stent restenosis and
progression of the atherosclerosis, clogging the blood vessel again. For these
reasons it is necessary to develop new materials and strategies focused on the
prevention and correction of this pathology and its subsequent complications.
In the present work, a reduction and in situ nitrogen doping method in graphene
oxide (N-rGO) was studied. By means of XPS a 9.57% at of nitrogen doping was
demonstrated and was found that the pyrrole type nitrogen is predominant. Due
to nitrogen doping and pyrrolic sites, the N-rGO surface reached high levels of
negative electric charge, which was verified by Electrostatic Force Microscopy
(EFM) and wettability tests between the LDL at different oxidation levels and
the synthesized N-rGO; contact angles between 95.7{\deg} and 130.4{\deg} were
obtained which indicates repulsion between both materials. The N-rGO was also
characterized by SEM and TEM where a wrinkled morphology typical of this type
of material was observed and the hexagonal atomic arrangement of GO is
distinguished. Finally, cell viability tests using
3-(4,5-dimethylthiazolyl)-2,5-diphenyltetrazolium bromide (MTT) assay were
carried out on human umbilical cord endothelial cells (HUVEC) to analyze the
cellular response at different concentrations of N-rGO, obtaining favorable
behavior up to concentrations of 100 ({\mu}g/mL).
