  Background
  SAMtools and BCFtools are widely used programs for processing and analysing
high-throughput sequencing data.
  Findings
  The first version appeared online twelve years ago and has been maintained
and further developed ever since, with many new features and improvements added
over the years. The SAMtools and BCFtools packages represent a unique
collection of tools that have been used in numerous other software projects and
countless genomic pipelines.
  Conclusion
  Both SAMtools and BCFtools are freely available on GitHub under the
permissive MIT licence, free for both non-commercial and commercial use. Both
packages have been installed over a million times via Bioconda. The source code
and documentation are available from http://www.htslib.org.
