  The size of particles which can be trapped in optical tweezers ranges from
tens of nanometres to tens of micrometres. This size regime also includes large
single molecules. Here we present experiments demonstrating that optical
tweezers can be used to collect polyethylene oxide (PEO) molecules suspended in
water. The molecules that accumulate in the focal volume do not aggregate and
therefore represent a region of increased molecule concentration, which can be
controlled by the trapping potential. We also present a model which relates the
change in concentration to the trapping potential. Since many protein molecules
have molecular weights for which this method is applicable the effect may be
useful in assisting nucleation of protein crystals.
