  The self-organized pattern (SOP) phenomenon is prevalent in plasma, while
knowledge about SOP discharge affecting reactive species generated
plasma-activated media (PAM) for cancer therapy is poorly documented. The aim
of this study focused on the effect of SOP discharge modes on reactive oxygen
and nitrogen species (ROS, RNS) in He SOP plasma-activated media with different
conductivity (saline solution and deionized (DI) water), and employed them to
breast cancer MDA-MB-231 and pancreatic BxPC-3 cancer cells. Optical emission
spectrum and Fluorimetric analysis were used to identify and quantify ROS and
RNS generated in He SOP plasma-activated saline solution and DI water.
Furthermore, He SOP plasma discharge modes are capable of efficiently
controlling the ROS and RNS concentration in the plasma-activated saline
solution and DI water, which contribute to the cytotoxic effect. On the other
hand, stainless steel and copper were used as a lower electrode to compare
their effect on cell viability. Taken together, our findings provide insight
into potential mechanisms involved in cell death after treatment with He SOP
plasma-activated media.
