  In addition to chemical and mechanical interactions between cells
electromagnetic field produced by cells has been considered as another form of
signaling for cell-cell communication. The aim of this study is evaluation of
electromagnetic effects on viability of Adipose-derived stem cells (ADSCs)
without co-culturing. In this study, stem cells were isolated from human
adipose tissue enzymatically and proliferated in monolayer culture. Then,
5.(10^4) adipose-derived stem cells were cultured in each well of the test
plate. In the first row (4 wells), ADSCs as inducer cells were cultured in
DMEM1 with 10 ng/ml Fibroblast growth factor (FGF). In adjacent and the last
rows, ADSCs were cultured without FGF (as detector cells). After the three and
five days the viability of cells were evaluated. Moreover, ADSCs were cultured
in the same conditions but the inducer cells were placed once in the UV-filter
tube and once in the quartz tube to see whether there is electromagnetic
interaction among cells. Inducer cells caused significant cell proliferation in
adjacent row cells (p- value<0.01) in the fifth day. However, using the
UV-filter tube and quartz tube both reduced the effect of inducer cells on
adjacent cells significantly. As a conclusion, we could detect distant cellular
interaction (DCI) among adipose derived stem cells (ADSCs), but it was not
electromagnetic signaling. Our results show that ADSCs affect each other via
volatile signaling as a chemical distant cellular interaction (CDCI).
