  The extension of DNA confined to nanochannels has been studied intensively
and in detail. Yet quantitative comparisons between experiments and model
calculations are difficult because most theoretical predictions involve
undetermined prefactors, and because the model parameters (contour length, Kuhn
length, effective width) are difficult to compute reliably, leading to
substantial uncertainties. Here we use a recent asymptotically exact theory for
the DNA extension in the "extended de Gennes regime" that allows us to compare
experimental results with theory. For this purpose we performed new
experiments, measuring the mean DNA extension and its standard deviation while
varying the channel geometry, dye intercalation ratio, and ionic buffer
strength. The experimental results agree very well with theory at high ionic
strengths, indicating that the model parameters are reliable. At low ionic
strengths the agreement is less good. We discuss possible reasons. Our approach
allows, in principle, to measure the Kuhn length and effective width of a
single DNA molecule and more generally of semiflexible polymers in solution.
