  Upon the addition of multivalent cations, a giant DNA chain exhibits a large
discrete transition from an elongated coil into a folded compact state. We
performed single-chain observation of long DNAs in the presence of a
tetravalent cation (spermine), at various temperatures and monovalent salt
concentrations. We confirmed that the compact state is preferred at higher
temperatures and at lower monovalent salt concentrations. This result is
interpreted in terms of an increase in the net translational entropy of small
ions due to ionic exchange between higher and lower valence ions.
