  The base pair fluctuations and helix untwisting are examined for a circular
molecule. A realistic mesoscopic model including twisting degrees of freedom
and bending of the molecular axis is proposed. The computational method, based
on path integral techniques, simulates a distribution of topoisomers with
various twist numbers and finds the energetically most favorable molecular
conformation as a function of temperature. The method can predict helical
repeat, openings loci and bubble sizes for specific sequences in a broad
temperature range. Some results are presented for a short DNA circle recently
identified in mammalian cells.
