  Cold atmospheric plasma (CAP) has been emerged as a novel technology for
cancer treatment. CAP can directly treat cells and tissue but such direct
application is limited to skin or can be invoked as a supplement during open
surgery. In this study we report indirect plasma treatment using CAP discharged
in DI water using three gases as carriers (argon, helium and nitrogen). Plasma
stimulated water was applied to human breast cancer cell line (MDA-MB-231). MTT
assay tests showed that using argon plasma had the strongest effect on inducing
apoptosis in cultured human breast cancer cells. This result is attributed to
the elevated production of the reactive oxygen species and reactive nitrogen
species in water in the case of argon plasma.
