  Micro-fluidic pumps as well as artificial micro-swimmers are conveniently
realized exploiting phoretic solvent flows based on local gradients of
temperature, electrolyte concentration or pH. We here present a facile
micro-photometric method for monitoring pH gradients and demonstrate its
performance and scope on different experimental situations including an
electro-osmotic pump and modular micro-swimmers assembled from ion exchange
resin beads and polystyrene colloids. In combination with the present
microscope and DSLR camera our method offers a 2 \mu m spatial resolution at
video frame rate over a field of view of 3920x2602 \mu m^2. Under optimal
conditions we achieve a pH-resolution of 0.05 with about equal contributions
from statistical and systematical uncertainties. Our quantitative
micro-photometric characterization of pH gradients which develop in time and
reach out several mm is anticipated to provide valuable input for reliable
modeling and simulations of a large variety of complex flow situations
involving pH-gradients including artificial micro-swimmers, microfluidic
pumping or even electro-convection.
