  We discuss scenarios with wrong-sign (WS) Yukawa couplings for the discovered
Higgs boson in the Yukawa-aligned two Higgs doublet model. In the WS scenario,
Yukawa couplings for down-type quarks and/or charged leptons have an opposite
sign as compared to those of the Higgs boson in the standard model, which can
be consistent with current flavour data and the Higgs signal strengths. The
phenomenology of additional Higgs bosons in such a scenario can be
significantly different from that with right-sign Yukawa couplings, mainly due
to a larger Higgs boson mixing to be required in the wrong-sign case. We show
the parameter space which is excluded or explored by direct searches for the
additional Higgs bosons at the current and high-luminosity LHC under the
constraints from perturbative unitarity and vacuum stability. In particular, we
find that most of the parameter space is explored in the WS scenario with the
Type-X (lepton specific) Yukawa interaction which is a special case of the
Yukawa alignment realized by imposing a softly-broken $\mathbb{Z}_2$ symmetry.
We propose that multi-Higgs events from pair productions of the additional
Higgs bosons can be the smoking gun signature to probe the WS scenario, and
give the expected number of events at the high-luminosity LHC.
