  We propose a method of spatial resolution enhancement in metrology
(thermometry, magnetometry, pH estimation and similar methods) with blinking
fluorescent nanosensors by combining sensing with super-resolution optical
fluctuation imaging (SOFI). To demonstrate efficiency of this approach, a model
experiment with laser diodes modeling fluctuating nanoemitters and intentional
blurring of the image is performed. The 2nd, 3rd, and 4th order cumulant images
provide improvement of the contrast and enable successful reconstruction of
smaller features of the modeled temperature (or any other physical parameter)
distribution relatively to the intensity-based approach. We believe that
blinking fluorescent sensing agents being complemented with the developed image
analysis technique could be utilized routinely in the life science sector for
recognizing the local changes in the spectral response of blinking
fluorophores, e.g. delivered targetly to the wanted cell or even organelle. It
is extremely useful for the local measurements of living cells' physical
parameters changes due to applying any external "forces", including disease
effect, aging, healing or response to the treatment.
