  We examine 20 diffuse and translucent Galactic sight lines and extract the
column densities of the ^12CO and ^13CO isotopologues from their ultraviolet
A--X absorption bands detected in archival Space Telescope Imaging Spectrograph
data with lambda/Deltalambda geq 46,000. Five more targets with Goddard
High-Resolution Spectrograph data are added to the sample that more than
doubles the number of sight lines with published Hubble Space Telescope
observations of ^13CO. Most sight lines have 12-to-13 isotopic ratios that are
not significantly different from the local value of 70 for ^12C/^13C, which is
based on mm-wave observations of rotational lines in emission from CO and H_2CO
inside dense molecular clouds, as well as on results from optical measurements
of CH^+. Five of the 25 sight lines are found to be fractionated toward lower
12-to-13 values, while three sight lines in the sample are fractionated toward
higher ratios, signaling the predominance of either isotopic charge exchange or
selective photodissociation, respectively. There are no obvious trends of the
^12CO-to-^13CO ratio with physical conditions such as gas temperature or
density, yet ^12CO/^13CO does vary in a complicated manner with the column
density of either CO isotopologue, owing to varying levels of competition
between isotopic charge exchange and selective photodissociation in the
fractionation of CO. Finally, rotational temperatures of H_2 show that all
sight lines with detected amounts of ^13CO pass through gas that is on average
colder by 20 K than the gas without ^13CO. This colder gas is also sampled by
CN and C_2 molecules, the latter indicating gas kinetic temperatures of only 28
K, enough to facilitate an efficient charge exchange reaction that lowers the
value of ^12CO/^13CO.
