  Standard model of electropration (EP) has long emphasized a single pore
lifetime to explain post-pulse transport across cell membranes. However, pore
lifetimes estimated from molecular dynamics (MD) models and and those measured
from experimental data differ by several orders of magnitude. We hypothesize
that a broad distribution of lifetimes may describe the post-pulse behavior.
Here, we show that pore distribution, number and size of pores, show
interesting behavior in different ranges of pore lifetimes. Interestingly, for
large electric fields (greater than 1 kV/cm) and short pore lifetimes
(approximately 100 ns), an significant loss in pore number occurs during the
pulse. Given the large number of EP applications that apply such fields, this
phenomenon may be crucial to post-pulse response of cell membrane to such
fields.
