  We have developed a new technique to measure viscoelasticity in soft
materials such as polymer solutions, by monitoring thermal fluctuations of
embedded probe particles using laser interferometry in a microscope.
Interferometry allows us to obtain power spectra of fluctuating beads from 0.1
Hz to 20 kHz, and with sub-nanometer spatial resolution. Using linear response
theory, we determined the frequency-dependent loss and storage shear moduli up
to frequencies on the order of a kHz. Our technique measures local values of
the viscoelastic response, without actively straining the system, and is
especially suited to soft biopolymer networks. We studied semiflexible F-actin
solutions and, as a control, flexible polyacrylamide (PAAm) gels, the latter
close to their gelation threshold. With small particles, we could probe the
transition from macroscopic viscoelasticity to more complex microscopic
dynamics. In the macroscopic limit we find shear moduli at 0.1 Hz of G'=0.11
+/- 0.03 Pa and 0.17 +/- 0.07 Pa for 1 and 2 mg/ml actin solutions, close to
the onset of the elastic plateau, and scaling behavior consistent with G(omega)
as omega^(3/4) at higher frequencies. For polyacrylamide we measured plateau
moduli of 2.0, 24, 100 and 280 Pa for crosslinked gels of 2, 2.5, 3 and 5%
concentration (weight/volume) respectively, in agreement to within a factor of
two with values obtained from conventional rheology. We also found evidence for
scaling of G(omega) as \omega^(1/2), consistent with the predictions of the
Rouse model for flexible polymers.
