  This study details the use of printing and other additive processes to
fabricate a novel amperometric glucose sensor. The sensor was fabricated using
a Au coated 12.7 micron polyimide film as a starting material, where
micro-contact printing, electrochemical plating and chloridization,
electrohydrodynamic jet (e-jet) printing, and spin coating were used to
pattern, deposit, print, and coat functional materials, respectively. We have
found that e-jet printing was effective for the deposition and patterning of
glucose oxidase inks between ~5 to 1000 micron in width, and we have
demonstrated that the enzyme was still active after printing. The thickness of
the permselective layer was optimized to obtain a linear response to glucose
concentration up to 32 mM. For these sensors no response to acetaminophen, a
common interfering compound, was observed.
