  Obesity and related type 2 diabetes are associated with a low level chronic
inflammatory state with disease status increasing at epidemic proportions
worldwide. It is now universally accepted that the underlying
immune-inflammatory responses mediated within the adipose tissue in obesity are
central to the development of disease. Once initiated, chronic inflammation
associated with obesity leads to the modulation of immune cell function. In the
present study we aimed to investigate the effect of an ayurvedic formulation
(named as Kal-1, an abbreviation derived from the procuring source) on
diet-induced obesity and type II diabetes using C57BL/6J mice. The study was
planned into two groups using obese and pre-diabetic mouse model. The mice were
fed on high-fat with increased diet and, different amounts (5, 20 and 75
{\mu}g) of Kal-1 were given with disease progression for 21 weeks in first
group whereas mice were first put on the same diet for 21 weeks and then
treated with same amounts of Kal-1. A significant reduction in body weight and
fat pads, fasting blood glucose levels, insulin levels and biochemical
parameters like triglycerides and cholesterol were observed in obese and
diabetic mice+Kal-1 than control (lean) mice fed on low fat diet, though the
optimum amounts of Kal-1 were 20 and 75 {\mu}g in first and second group
respectively. A noteworthy immunological correction in important readouts viz.
resistin, leptin, HMW adiponection and an array of pro- & anti-cytokines (IL-4,
IL-10, IL-1{\alpha}, IL-1{\beta}, IL-4, IL-6, IL-10, TNF-{\alpha} and MCP-1)
was also observed in both the groups with the mentioned amount of Kal-1. We
conclude that Kal-1 has immunomodulatory potential for diet-induced obesity and
associated metabolic disorders.
