  Electron cryotomography (CryoET) is currently the only method capable of
visualizing cells in 3D at nanometer resolutions. While modern instruments
produce massive amounts of tomography data containing extremely rich structural
information, the data processing is very labor intensive and results are often
limited by the skills of the personnel rather than the data. We present an
integrated workflow that covers the entire tomography data processing pipeline,
from automated tilt series alignment to subnanometer resolution subtomogram
averaging. This workflow greatly reduces human effort and increases throughput,
and is capable of determining protein structures at state-of-the-art
resolutions for both purified macromolecules and cells.
