  Despite super-resolution fluorescence blinking microscopes break the
diffraction limit, the intense phototoxic illumination and long-term image
sequences thus far still pose to major challenges in visualizing
live-organisms. Here, we proposed a super-resolution method based on
auto-correlation two-step deconvolution (SACD) to enhance the temporal
resolution at lower signal intensity levels. Unlike conventional techniques,
such as super-resolution optical fluctuation imaging (SOFI) or stochastic
optical reconstruction microscopy (STORM), our model allows 16 frames to
generate super-resolution images, without noticeable degradation in recording
quality. We demonstrate SACD both in simulated predictions and experimental
validations, with the resulting spatial resolution of 64 nm and 2-10 fold speed
improvements. The use of low signal-to-noise ratio acquisition of image
sequences, our SACD enables fast, background-free, super-resolution imaging
that maybe advance implemented as a suitable tool for rapid live-cells imaging.
