  The total phenols and flavonoids content of different polar solvent extracts
from Aurea Helianthus flowers, and their antioxidant activity were determined.
The ethanol extract of Aurea Helianthus flowers were suspended in water and
fractionated using different polar solvents; hexane, chloroform, ethyl acetate,
butanol and water. The parameters of each extract mentioned above were
determined using Floin-ciocalteu reagent(FCR) method, AlCl3 colorimetry method,
ferric reducing ability of plasma(FRAP) assay, total antioxidant activity(TAA)
assay and DPPH radical scavenging assay. The highest total phenols
content(516.21 mg GAE/g) and flavonoids content(326.06 mg QCE/g) were obtained
in ethyl acetate extract, the correlation between TPC and TFC assay was founded
to be 0.967. All polar solvent extracts of Aurea Helianthus flowers showed
significant antioxidant effects, the hightest inhibition was obtained in ethyl
acetate and choroform extracts and the lowest inhibition in the water extract.
There is a good correlation of total phenols and flavonoids content with
antioxidant activity. This work indicated that the polar solvent extracts of
Aurea Helianthus flowers contain high phenols and flavonoids content and
exhibited antioxidant activities in vitro, therefore, could be candidates for
use as natural antioxidant.
