  The entire T7 bacteriophage genome contains 39937 base pairs (Database NCBI
RefSeq N1001604). Here, electrostatic potential distribution around double
helical T7 DNA was calculated by Coulomb method using the computer program of
Sorokin A.A. Electrostatic profiles of 17 promoters recognized by T7 phage
specific RNA polymerase were analyzed. It was shown that electrostatic profiles
of all T7 RNA polymerase specific promoters can be characterized by distinctive
motifs which are specific for each promoter class. Comparative analysis of
electrostatic profiles of native T7 promoters of different classes demonstrates
that T7 RNA polymerase can differentiate them due to their electrostatic
features.
