  Let $v(F)$ denote the number of vertices in a fixed connected pattern graph
$F$. We show an infinite family of patterns $F$ such that the existence of a
subgraph isomorphic to $F$ is expressible by a first-order sentence of
quantifier depth $\frac23\,v(F)+1$, assuming that the host graph is
sufficiently large and connected. On the other hand, this is impossible for any
$F$ with using less than $\frac23\,v(F)-2$ first-order variables.
