  We complete the program of 2012.15792 about perturbative approaches for
$\mathcal{N}=2$ superconformal quiver theories in four dimensions. We consider
several classes of observables in presence of Wilson loops, and we evaluate
them with the help of supersymmetric localization. We compute Wilson loop
vacuum expectation values, correlators of multiple coincident Wilson loops and
one-point functions of chiral operators in presence of them acting as
superconformal defects. We extend this analysis to the most general case
considering chiral operators and multiple Wilson loops scattered in all the
possible ways among the vector multiplets of the quiver. Finally, we identify
twisted and untwisted observables which probe the orbifold of $AdS_5\times S^5$
with the aim of testing possible holographic perspectives of quiver theories in
$\mathcal{N}=2$.
