  Keratin are among the most abundant proteins in epithelial cells. Functions
of the keratin network in cells are shaped by their dynamical organization.
Using a collection of experimentally-driven mathematical models, different
hypotheses for the turnover and transport of the keratin material in epithelial
cells are tested. The interplay between turnover and transport and their
effects on the keratin organization in cells are hence investigated by
combining mathematical modeling and experimental data. Amongst the collection
of mathematical models considered, a best model strongly supported by
experimental data is identified. Fundamental to this approach is the fact that
optimal parameter values associated with the best fit for each model are
established. The best candidate among the best fits is characterized by the
disassembly of the assembled keratin material in the perinuclear region and an
active transport of the assembled keratin. Our study shows that an active
transport of the assembled keratin is required to explain the experimentally
observed keratin organization.
