  Antibactrial activity of Asteriscus graveolens methanolic extract and its
synergy effect with fungal mediated silver nanoparticles (AgNPs) against some
enteric bacterial human pathogen was conducted. Silver nanoparticles were
synthesized by the fungal strain namely Tritirachium oryzae W5H as reported
early. In this study, MICs of AgNPs against E. aerogenes, Salmonella sp., E.
coli and C. albicans in order were 2.13, 19.15, 0.08 and 6.38 micrograms per
mL, respectively, while the MICs of A. graveolens ethanolic extract against the
same bacteria were 4, 366, 3300 and 40 micrograms per mL, respectively. The MIC
values at concentration less than 19.15 and 40 micrograms per mL indicating the
potent bacteriostatic effect of AgNPs and A. graveolens ethanolic
extract.Increasing in IFA was reported when Nitrofurantion and Trimethoprim
were combined with Etoh extract with maximum increase in IFA by 6 and 12 folds
for, respectively. Also, 10 folds increasing in IFA was reported when
trimethoprim was combined with AgNPs: Etoh extract.But, there were no
synergistic effect between the antifungal agents (Caspofungin and Micafungin)
combined with AgNPs and or A. graveolens ethanolic extract against C.
albicans.The potent synergistic effect of A. graveolens ethanolic extract
and/or NPs with the conventional antibiotics is novel in inhibiting antibiotics
resistant bacteria. In this study, remarkable increasing in the antibacterial
activity, when the most resistant antibiotics combined with A. graveolense
thanolic extract and/or NPs was reported.
