  The negatively charged nitrogen-vacancy centre (NV$^-$) in diamond has been
utilized in a wide variety of sensing applications. The centre's long spin
coherence and relaxation times ($T_2^*$, $T_2$ and $T_1$) at room temperature
are crucial to this, as they often limit sensitivity. Using NV$^-$ centres in
nanodiamonds allows for operations in environments inaccessible to bulk
diamond, such as intracellular sensing. We report long spin coherence and
relaxation times at room temperature for single NV$^-$ centres in
isotopically-purified polycrystalline ball-milled nanodiamonds. Using a
spin-locking pulse sequence, we observe spin coherence times, $T_2$, up 786
$\pm$ 200 $\mu$s. We also measure $T_2^*$ times up to 2.06 $\pm$ 0.24 $\mu$s
and $T_1$ times up to 4.32 $\pm$ 0.60 ms. Scanning electron microscopy and
atomic force microscopy measurements show that the diamond containing the
NV$^{-}$ centre with the longest $T_1$ time is smaller than 100 nm. EPR
measurements give an N$_{s}$$^{0}$ concentration of 0.15 $\pm$ 0.02 ppm for the
nanodiamond sample.
