  Microbes are essentially yet convolutedly linked with human lives on the
earth. They critically interfere in different physiological processes and thus
influence overall health status. Studying microbial species is used to be
constrained to those that can be cultured in the lab. But it excluded a huge
portion of the microbiome that could not survive on lab conditions. In the past
few years, the culture-independent metagenomic sequencing enabled us to explore
the complex microbial community coexisting within and on us. Metagenomics has
equipped us with new avenues of investigating the microbiome, from studying a
single species to a complex community in a dynamic ecosystem. Thus, identifying
the involved microbes and their genomes becomes one of the core tasks in
metagenomic sequencing. Metagenome-assembled genomes are groups of contigs with
similar sequence characteristics from de novo assembly and could represent the
microbial genomes from metagenomic sequencing. In this paper, we reviewed a
spectrum of tools for producing and annotating metagenome-assembled genomes
from metagenomic sequencing data and discussed their technical and biological
perspectives.
