  The most realistic information about the transparent sample such as a live
cell can be obtained only using bright-field light microscopy. At
high-intensity pulsing LED illumination, we captured a primary
12-bit-per-channel (bpc) response from an observed sample using a bright-field
microscope equipped with a high-resolution (4872x3248) image sensor. In order
to suppress data distortions originating from the light interactions with
elements in the optical path, poor sensor reproduction (geometrical defects of
the camera sensor and some peculiarities of sensor sensitivity), we propose a
spectroscopic approach for the correction of this uncompressed 12-bpc data by
simultaneous calibration of all parts of the experimental arrangement.
Moreover, the final intensities of the corrected images are proportional to the
photon fluxes detected by a camera sensor. It can be visualized in 8-bpc
intensity depth after the Least Information Loss compression.
