  Suillus species, in general, are edible mushrooms, and environmentally
important that are associated mostly with pine trees in the tropics regions.
These fungi considered a remarkable source of phenolic compounds that play a
crucial role as antioxidants which may reduce the risk of most human chronic
diseases such as cancer, diabetes, asthma, atherosclerosis, Alzheimer, and
others. On the other hand, carotenoids (\b{eta} carotene) are the most popular
natural pigments which play an important role to protect the plants from
photo-oxidative reactions. In human, these compounds prevent oxidative stress
and expects to have antimicrobial activity. Here, the phenolic compounds were
extracted with Ethyl acetate from fruiting bodies of Suillus sp and analyzed by
HPLC, the antioxidant activity (reducing power%) of phenolic compounds was
determined at the concentrations of 1, 2.5, and 5 mg/mL. Antimicrobial activity
of \b{eta} carotene pigment was measured at a concentration of 100 mg/mL
against some human pathogenic bacteria such as Escherichia coli, Pseudomonas
aeruginosa, Klebsiella pneumonia, and Staphylococcus aureus. The specific DNA
region ITS was amplified and sequenced using ITS1 and ITS4 primers with some
bioinformatics analyses. The phenolic extract isolated from fruiting bodies of
Suillus sp showed a remarkable antioxidant activity by increasing the reducing
power percent (from F+3 ions to F+2 ions) comparing with the industrial
antioxidant (Propyl gallate) at all used concentrations. Percent of reducing
power of phenolic compounds were 75.5, 84.9 and 95.7% at concentrations of 1,
2.5, and 5 mg/mL respectively; comparing with PG were 65.9, 81.3, and 93.3 at
1, 2.5, and 5 mg/mL respectively. The \b{eta} carotene pigment revealed a
significant antimicrobial activity at a concentration of 100 mg/mL against K.
pneumonia, E. coli, and S. aureus.
