  Various techniques have been developed to measure the 2D and 3D positions and
2D and 3D orientations of fluorescent molecules with improved precision over
standard epifluorescence microscopes. Due to the challenging
signal-to-background ratio in typical single-molecule experiments, it is
essential to choose an imaging system optimized for the specific target sample.
In this work, we compare the performance of multiple state-of-the-art and
commonly used methods for orientation localization microscopy against the
fundamental limits of measurement precision. Our analysis reveals optimal
imaging methods for various experiment conditions and sample geometries.
Interestingly, simple modifications to the standard fluorescence microscope
exhibit superior performance in many imaging scenarios.
