  A rapid and accurate numerical solution to the transport equation (i.e.,
inverse adding doubling) of diffuse reflectance has been utilized here for the
calculation of optical properties of the Yucatan miniature pigskin as human
surrogate skin. This method is valid for a wide range of optical properties and
can be adapted to a variety of probe geometries, provided calibration
measurements are made. Inverse adding doubling (IAD), based on the forward
solution to the transport equation is a numerical solution in which optical
properties can be used as a prior for computing other properties for turbid
media. In this work, integrating spheres with fitted laser diodes and a
monochromator was used experimentally as the irradiation source. A
cryo-microtome was used to precisely slice tissue samples. Confocal and atomic
force microscopy optical methods were used to visualize tissue
post-irradiation. The results from this work offer an additional method to
determine the optical properties of biological tissues.
