  A technique of pulsed low-energy electron holography is introduced that
allows for recording highly resolved holograms within reduced exposure times.
Therefore, stacks of holograms are accumulated in a pulsed mode with individual
acquisition times as short as 50 {\mu}s. Subsequently, these holograms are
aligned and finally superimposed. The resulting holographic record reveals
previously latent high-order interference fringes and thereby pushing
interference resolution into the sub-nanometer regime. In view of the
non-damaging character of low-energy electrons, the method is of particular
interest for structural analysis of fragile biomolecules.
