  Microfluidic technology has emerged as a powerful tool for studying complex
biological processes with enhanced precision and control. A microfluidic chip
was designed to emulate human-like microvascular networks with precise control
over channel geometry and flow conditions. By simulating blood flow dynamics
during bleeding events, we successfully observed the real-time interactions of
platelets and their aggregation induced by shear rate gradient at the wound
site. Platelet dynamics is primarily influenced by physico-mechanical condition
of blood vessels with pathophysiological condition of blood at close proximity
of vascular injury site. This microfluidic platform facilitated the
investigation of platelet adhesion, activation, and clot formation, providing a
unique opportunity to study the spatiotemporal dynamics of platelet aggregation
and blood clot. Our findings shed light on the intricate mechanisms underlying
thrombus formation and platelet-mediated aggregation, offering a more accurate
and dynamic representation of human haemostasis compared to traditional animal
models. In the conventional approach, the human bleeding model is tried on
mouse due to anatomy and pathological similarities between mouse and humans.
This study will simplify and standardize the blood and vasculature conditions.
The microfluidic-based replication of the bleeding model holds significant
promise in advancing our understanding of clotting disorders and wound healing
processes. Furthermore, it paves the way for targeted therapeutic interventions
in managing bleeding disorders and enhancing clinical strategies for promoting
efficient wound closure. Ultimately, this study demonstrates the potential of
microfluidics to revolutionize haemostasis research and opens up new avenues
for the development of personalized medicine approaches in the field of
clotting disorders.
