  This study aimed to evaluate the potential of 3D echo-planar imaging (EPI)
for improving the reliability of $T_2^*$-weighted ($T_2^*w$) data and
quantification of $\textit{R}_2^*$ decay rate and susceptibility ($\chi$)
compared to conventional gradient echo (GRE)-based acquisition. Eight healthy
subjects in a wide age range were recruited. Each subject received repeated
scans for both GRE and EPI acquisitions with an isotropic 1 mm resolution at 3
T. Maps of $\textit{R}_2^*$ and $\chi$ were quantified and compared using their
inter-scan difference to evaluate the test-retest reliability. Inter-protocol
differences of $\textit{R}_2^*$ and $\chi$ between GRE and EPI were also
measured voxel by voxel and in selected ROIs to test the consistency between
the two acquisition methods. The quantifications of $\textit{R}_2^*$ and $\chi$
using EPI protocols showed increased test-retest reliability with higher EPI
factors up to 5 as performed in the experiment and were consistent with those
based on GRE. This result suggested multi-shot multi-echo 3D EPI can be a
useful alternative acquisition method for $T_2^*w$ MRI and quantification of
$\textit{R}_2^*$ and $\chi$ with reduced scan time, improved test-retest
reliability and similar accuracy compared to commonly used 3D GRE.
