  We present the results of high temperature 500 K Molecular Dynamics
simulations of the DNA i-motif. The essential dynamics and the main unfolding
pathways are compared to a biased metadynamics simulation at 300 K. Our results
indicate a remarkable agreement of the concerted motion at both temperatures.
The transition can be described by a few number of eigenvectors indicating a
simple unfolding process. Two main mechanisms for the unfolding pathway at 500
K can be detected which are in good agreement to the results of the biased
simulation at 300 K.
