  In recent years, substantial advancements have been achieved in understanding
the diversity of the human virome and its intricate roles in human health and
diseases. Despite this progress, the field of human virome research remains
nascent, primarily hindered by the absence of effective methods, particularly
in the domain of computational tools. This perspective systematically outlines
ten computational challenges spanning various phases of virome studies, ranging
from virus identification, sequencing quality evaluation, genome assembly,
annotation of viral taxonomy, genome and proteins, inference of biological
properties, applications of the virome in disease diagnosis, interactions with
other microbes, and associations with human diseases. The resolution of these
challenges necessitates ongoing collaboration among computational scientists,
virologists, and multidisciplinary experts. In essence, this perspective serves
as a comprehensive guide for directing computational efforts in human virome
studies, aiming to significantly propel the field forward.
