  Optical tomographic imaging of biological specimen bases its reliability on
the combination of both accurate experimental measures and advanced
computational techniques. In general, due to high scattering and absorption in
most of the tissues, multi view geometries are required to reduce diffuse halo
and blurring in the reconstructions. Scanning processes are used to acquire the
data but they inevitably introduces perturbation, negating the assumption of
aligned measures. Here we propose an innovative, registration free, imaging
protocol implemented to image a human tumor spheroid at mesoscopic regime. The
technique relies on the calculation of autocorrelation sinogram and object
autocorrelation, finalizing the tomographic reconstruction via a three
dimensional Gerchberg Saxton algorithm that retrieves the missing phase
information. Our method is conceptually simple and focuses on single image
acquisition, regardless of the specimen position in the camera plane. We
demonstrate increased deep resolution abilities, not achievable with the
current approaches, rendering the data alignment process obsolete.
