  Summary: Hydrogen deuterium exchange mass spectrometry (HDX-MS) is becoming
increasing routine for monitoring changes in the structural dynamics of
proteins. Differential HDX-MS allows comparison of individual protein states,
such as in the absence or presence of a ligand. This can be used to attribute
changes in conformation to binding events, allowing the mapping of entire
con-formational networks. As such, the number of necessary cross-state
comparisons quickly increas-es as additional states are introduced to the
system of study. There are currently very few software packages available that
offer quick and informative comparison of HDX-MS datasets and even few-er which
offer statistical analysis and advanced visualization. Following the feedback
from our origi-nal software Deuteros, we present Deuteros 2.0 which has been
redesigned from the ground up to fulfil a greater role in the HDX-MS analysis
pipeline. Deuteros 2.0 features a repertoire of facilities for back exchange
correction, data summarization, peptide-level statistical analysis and advanced
data plotting features. Availability: Deuteros 2.0 can be downloaded from
https://github.com/andymlau/Deuteros_2.0 under the Apache 2.0 license.
Installation of Deuteros 2.0 requires the MATLAB Runtime Library available free
of charge from MathWorks
(https://www.mathworks.com/products/compiler/matlab-runtime.html) and is
available for both Windows and Mac operating systems.
