  Super-resolution microscopes (such as STED) illuminate samples with a tiny
spot, and achieve very high resolution. But structures smaller than the spot
cannot be resolved in this way. Therefore, we propose a technique to solve this
problem. It is termed "Deconvolution after Dense Scan (DDS)". First, a
preprocessing stage is introduced to eliminate the optical uncertainty of the
peripheral areas around the sample's ROI (Region of Interest). Then, the ROI is
scanned densely together with its peripheral areas. Finally, the high
resolution image is recovered by deconvolution. The proposed technique does not
need to modify the apparatus much, and is mainly performed by algorithm.
Simulation experiments show that the technique can further improve the
resolution of super-resolution microscopes.
