  Histological examination is crucial for cancer diagnosis, including
hematoxylin and eosin (H&E) staining for mapping morphology and
immunohistochemistry (IHC) staining for revealing chemical information.
Recently developed two-color stimulated Raman histology could bypass the
complex tissue processing to mimic H&E-like morphology. Yet, the underlying
chemical features are not revealed, compromising the effectiveness of
prognostic stratification. Here, we present a high-content stimulated Raman
histology (HC-SRH) platform that provides both morphological and chemical
information for cancer diagnosis based on un-stained breast tissues. Through
spectral unmixing in the C-H vibration window, HC-SRH can map unsaturated
lipids, cellular protein, extracellular matrix, saturated lipid, and water in
breast tissue. In this way, HC-SRH provides excellent contrast for various
tissue components. Considering rapidness is important in clinical trials, we
implemented spectral selective sampling to boost the speed of HC-SRH by one
order. We also successfully demonstrated the HC-SRH in a clinical-compatible
fiber laser-based SRS microscopy. With the widely rapid tuning capability of
the advanced fiber laser, a clear chemical contrast of nucleic acid and
solid-state ester is shown in the fingerprint result.
