  Super-resolution fluorescence microscopy provides unprecedented insight into
cellular and subcellular structures. However, going "beyond the diffraction
barrier" comes at a price since most far-field super-resolution imaging
techniques trade temporal for spatial super-resolution. We propose the
combination of a novel label-free white light quantitative phase tomography
with fluorescence imaging to provide high-speed imaging and spatial
super-resolution. The non-iterative phase reconstruction relies on the
acquisition of single images at each z-location and thus enables
straightforward 3D phase imaging using a classical microscope. We realized
multi-plane imaging using a customized prism for the simultaneous acquisition
of 8 planes. This allowed us to not only image live cells in 3D at up to 200
Hz, but also to integrate fluorescence super-resolution optical fluctuation
imaging within the same optical instrument. This 4D microscope platform unifies
the sensitivity and high temporal resolution of phase tomography with the
specificity and high spatial resolution of fluorescence imaging.
