  The latest trends in cancer research and nanomedicine focus on using
nanocarriers to target cancer stem cells (CSCs). Specifically, lipid liquid
nanocapsules are usually developed as nanocarriers for lipophilic drug
delivery. Here, we developed olive oil liquid NCs (O2LNCs) functionalized by
covalent coupling of an anti-CD44-fluorescein isothiocyanate antibody
({\alpha}CD44). First, O2LNCs are formed by a core of olive oil surrounded by a
shell containing phospholipids, a nonionic surfactant, and deoxycholic acid
molecules. Then, O2LNCs were coated with an {\alpha}CD44 antibody
({\alpha}CD44-O2LNC). The optimization of an {\alpha}CD44 coating procedure, a
complete physicochemical characterization, as well as clear evidence of their
efficacy in vitro and in vivo were demonstrated. Our results indicate the high
targeted uptake of these {\alpha}CD44-O2LNCs, and the increased antitumor
efficacy (up to four times) of paclitaxel-loaded-{\alpha}CD44-O2LNC compared to
free paclitaxel in pancreatic CSCs (PCSCs). Also, {\alpha}CD44-O2LNCs were able
to selectively target PCSCs in an orthotopic xenotransplant in vivo model.
