  DNA constructs and their annotated sequence maps have been rapidly
accumulating with the advancement of DNA cloning, synthesis, and assembly
methods. Such a resource has the potential to be optimally utilized in an
autonomous DNA building platform. However, most DNA design processes today
remain manually operated with the assistance of graphical user interface (GUI)
software. Furthermore, as seen commonly in the life sciences, reproducibility
of DNA construction process descriptions is usually not guaranteed, and
utilization of previously developed materials and protocols is not
appropriately credited. Here, we developed an open-source process description
and resource sharing framework QUEEN (a framework to generate quinable and
efficiently editable nucleotide sequence resources) to resolve these issues in
building DNA. QUEEN enables the flexible design of new DNA by using existing
DNA resource files and recoding the construction process in an output file
(GenBank file format). The GenBank files generated by QUEEN are able to
regenerate the process codes that perfectly clone themselves and bequeath the
design history to successive DNA constructs that recycle their partial
resources. QUEEN-generated GenBank files are compatible with the existing DNA
repository services and software. We propose QUEEN as a solution to start
significantly advancing our material and protocol sharing of DNA resources.
