  Experiments using multi-step protocols often involve several restrictions on
the randomization. For a specific application to in vitro testing on
microplates, a design was required with both a split-plot and a strip-plot
structure. On top of two-level treatment factors and the factors that define
the randomization restrictions, a multi-level fixed blocking factor not
involving further restrictions on the randomization had to be added. We develop
a step-by-step approach to construct a design for the microplate experiment and
analyze a response. To consolidate the approach, we study various alternative
scenarios for the experiment.
