  The recent work by Chmyrov et al. (Nature Methods 2013) presents a capstone
for the current major super-resolution microscopy techniques. In optical
super-resolution microscopy, two pathways are commonly taken: targeted
illumination modulation, or stochastic single-molecule localization. Incoherent
cross-standing microscopy has utilized the concept of structured illumination
in generating patterns, to generate effective 100,000 "doughnuts" as with STED;
and used the photo-switchable dye to decrease the requirement of modulation
intensity. It has combined the key elements of all these major super-resolution
techniques.
