  Electrokinetic force has been the major choice for driving the translocation
of molecules through a nanopore. However, the use of this approach is limited
by an uncontrollable translocation speed, resulting in non-uniform conductance
signals with low conformational sensitivity, which hinders the accurate
discrimination of the molecules. Here, we show the first use of
inertial-kinetic translocation induced by spinning an in-tube micro-pyramidal
silicon nanopore fabricated using photovoltaic electrochemical etch-stop
technique for biomolecular sensing. By adjusting the kinetic properties of a
funnel-shaped centrifugal force field while maintaining a counter-balanced
state of electrophoretic and electroosmotic effect in the nanopore, we achieved
regulated translocation of proteins and obtained stable signals of long and
adjustable dwell times and high conformational sensitivity. Moreover, we
demonstrated instantaneous sensing and discrimination of molecular
conformations and longitudinal monitoring of molecular reactions and
conformation changes by wirelessly measuring characteristic features in current
blockade readouts using the in-tube nanopore device.
