  Real-time deformability cytometry (RT-DC) is an established method that
quantifies features like size, shape, and stiffness for whole cell populations
on a single-cell level in real time. To extract the cell stiffness, a lookup
table (LUT) disentangles the experimentally derived steady state cell
deformation and the projected area, yielding the Young's modulus. So far, two
lookup tables exists, but are limited to simple linear material models and
cylindrical channel geometries. Here, we present two new lookup tables for
RT-DC based on a neo-Hookean hyperelastic material numerically derived by
simulations based on the finite element method in square and cylindrical
channel geometries. At the same time, we quantify the influence of the
shear-thinning behaviour of the surrounding medium on the stationary
deformation of cells in RT-DC and discuss the applicability and impact of the
proposed LUTs regarding past and future RT-DC data analysis. Additionally, we
provide insights about the cell strain and stresses, as well as the influence
resulting from the rotational symmetric assumption on the cell deformation and
volume estimation. The new lookup tables as well as the numerical cell shapes
are made freely available.
