  We collaborated with Amgen and spent five years in designing and fabricating
next generation multi-well plate imagers based on Fourier ptychographic
microscopy (FPM). A 6-well imager (Emsight) and a low-cost parallel microscopic
system (96 Eyes) based on parallel FPM were reported in our previous work.
However, the effect of liquid meniscus on the image quality is much stronger
than anticipated, introducing obvious wavevector misalignment and additional
image aberration. To this end, an adaptive wavevector correction (AWC-FPM)
algorithm and a pupil recovery improvement strategy are presented to solve
these challenges in situ. In addition, dual-channel fluorescence excitation is
added to obtain structural information for microbiologists. Experiments are
demonstrated to verify their performances. The accuracy of angular resolution
with our algorithm is within 0.003 rad. Our algorithms would make the FPM
algorithm more robust and practical and can be extended to other FPM-based
applications to overcome similar challenges.
