  Confined motions in complex environments are ubiquitous in microbiology.
These situations invariably involve the intricate coupling between fluid flow,
soft boundaries, surface forces and fluctuations. In the present study, such a
coupling is investigated using a novel method combining holographic microscopy
and advanced statistical inference. Specifically, the Brownian motion of
softmicrometric oil droplets near rigid walls is quantitatively analyzed. All
the key statistical observables are reconstructed with high precision, allowing
for nanoscale resolution of local mobilities and femtonewton inference of
conservative or non-conservative forces. Strikingly, the analysis reveals the
existence of a novel, transient, but large, soft Brownian force. The latter
might be of crucial importance for microbiological and nanophysical transport,
target finding or chemical reactions in crowded environments, and hence the
whole life machinery.
