  A novel technique was demonstrated that overcome important drawbacks to
crosslink cells by irradiation with ultrashort UV laser pulses
(L-crosslinking). To use this technique coupled to Chromatin
ImmunoPrecipitation (ChIP) in a high throughput context, a pre-screening fast
method needs to be implemented to set up suitable irradiation conditions of the
cell sample for efficient L-crosslinking with no final and long ChIP analysis.
Here a fast method is reported where living human cells have been first
transfected with a vector coding for Estrogen Receptor {\alpha} (ER{\alpha}),
linked to Green Florescent protein (ER{\alpha}-GFP), so that the well-known
interaction between the Estrogen Receptor Elements (ERE) region of the cell DNA
and the ER{\alpha} protein can be detected by studying the fluorometric
response of the irradiated cells. The damage induced to cells by UV irradiation
is characterized by looking at DNA integrity, proteins stability and cellular
viability. A second novel approach is presented to analyze or re-visit DNA and
RNA sequences and their molecular configurations. This approach is based on
methods derived from Chern-Simons super-gravity adapted to describe mutations
in DNA/RNA strings, as well as interactions between nucleic acids. As a
preliminary case we analyze the KRAS human gene sequence and some of its
mutations. Interestingly, our model shows how the Chern-Simons current are
capable to characterize the mutations within a sequence, in particular giving a
quantitative indication of the mutation likelihood.
