  Light scattering by small particles is one of the most prevailing and
non-invasive technique for examining the properties of particulate systems
chiefly of biological origin. The sub-micron particles including the
bio-particles were so chosen because of their importance in biology and
biomedical sciences. Light scattering investigation from homogenous, pathogenic
Pseudomonas aeruginosa and non-pathogenic Mycobacterium smegmatis was carried
out at two different wavelength of incident light, by using an original
designed and fabricated polar and azimuth-dependent light scattering setup.
Theoretical scattering plots were generated by using T-matrix approach to
validate our analyses. Simulations of light scattering of these particles were
also carried out using a novel Monte- Carlo simulation technique. The relation
between experimental, theoretical and simulated result is presented in this
paper. We have studied Pseudomonas aeruginosa and Mycobacterium smegmatis, and
attempted for prospect of observing its morphological property by using light
scattering tool.
