  The primary and metastatic stages of BC consider different cancerous cell
lines (MDAs), meaning the highest number of cells in this research (300,000)
represents the metastatic stages of BC, and 50,000 represents the primary level
of BC developments have been studied based on fluorescence-enhanced
photodynamic characterizations. The ability to characterize the fluorescence
caused by MDA with 50,000 cells compared to the dominant radiation of MDA with
300,000 cells is emphatic proof of the high potential of fluorescence technique
in timely BC detections. 5-ALA induces an accumulation of protoporphyrin IX
(PpIX) photosensitizer through a biosynthetic pathway, leading to red radiation
of fluorescence measurements. The fluorescence signal reduction due to
decreased cell viability and increased MDA's cellular death rate corresponds to
the changes in lipid metabolism enzymes of MDAs cultured at different doses.
Furthermore, statistical concerns have been studied using PCA multivariate
component analysis to differentiate MDA cells administrated by 5-ALA.
