  We present a high-throughput microfluidic platform capable of quantitating up
to 384 biomarkers in 4 distinct samples by immunoassay. The microfluidic device
contains 384 unit cells, which can be individually programmed with pairs of
capture and detection antibody. Samples are quantitated in each unit cell by
four independent MITOMI detection areas, allowing four samples to be analyzed
in parallel for a total of 1,536 assays per device. We show that the device can
be pre-assembled and stored for weeks at elevated temperature and we performed
proof-of-concept experiments simultaneously quantitating IL-6, IL-1\b{eta},
TNF-{\alpha}, PSA, and GFP. Finally, we show that the platform can be used to
identify functional antibody combinations by screening 64 antibody combinations
requiring up to 384 unique assays per device.
