  We present the first evidence of nitrogen vacancy (NV) photoluminescence from
a nanodiamond suspended in a free-space optical dipole trap at atmospheric
pressure. The photoluminescence rates are shown to decrease with increasing
trap laser power, but are inconsistent with a thermal quenching process. For a
continuous-wave trap, the neutral charge state (NV$^0$) appears to be
suppressed. Chopping the trap laser yields higher total count rates and results
in a mixture of both NV$^0$ and the negative charge state (NV$^-$).
