  In this manuscript we present an in vitro model based on agarose gel that can
be used to simulate a dirty, oily, bloody, and morphologically complex surface
of, for example, an open wound. We show this models effectiveness in simulating
depth of penetration of reactive species generated in plasma deep into tissue
of a rat and confirm the penetration depths with agarose gel model. We envision
that in the future such a model could be used to study plasma discharges (and
other modalities) and minimize the use of live animals: plasma can be optimized
on the agarose gel wound model and then finally verified using an actual wound.
