  Deuterated ions are abundant in cold (T=10 K), dense (n=10^5 cm^-3) regions,
in which CO is frozen out onto dust grains. In such environments, the deuterium
fractionation of such ions can exceed the elemental abundance ratio of D/H by a
factor of 10^4. In this paper we use the deuterium fractionation to investigate
the evolutionary state of Class 0 protostars. In a sample of 20 protostellar
objects, we found a clear correlation between the N2D+/N2H+ ratio and
evolutionary tracers. As expected, the coolest, i.e. the youngest, objects show
the largest deuterium fractionation. Furthermore, we find that sources with a
high N2D+/N2H+ ratio show clear indication for infall.
