  Regulating cell growth, extracellular matrix deposition and mineralization of
artificial implants are some important parameters that decide the longevity of
implants in the body. Picosecond laser induced hybrid groove structures have
been shown to improve these properties of the Ti6Al4V bio-alloy. Two hybrid
structures containing groove patterns with periodic and non-periodic
substructures therein were generated on Ti6Al4V by varying the extent of laser
pulse overlapping on sample surface. Laser induced alteration in surface
topography, chemical composition and wettability of Ti6Al4V resulted in 3-fold
increase in the rate of hydroxyapatite growth, 2.5-fold increment in protein
adsorption and 2-fold enhancement in cell adhesion in comparison to pristine
sample. While the periodic substructure was found to guide cell growth, the non
periodic sub structure offered homogenous growth leading to higher overall cell
density on the substrate surface.
