  Molecular dynamics (MD) studies of buffalo prion protein (BufPrP$^\text{C}$)
[Zhang JP et al.(2016) J Biomol Struct Dyn 34(4):762-777] showed that the
structure of this protein is very stable at room temperature (whether under
neutral pH or low pH environments). In order to understand the reason why
buffalo is lowly susceptible to prion diseases and why BufPrP$^\text{C}$ is so
stable at room temperature, this paper will prolong our MD running time at room
temperature and extend our research to higher temperatures to study this
BufPrP$^\text{C}$ structure furthermore. From the salt bridge point of view we
found an important reason why BufPrP$^\text{C}$ is so stable at room
temperature and this might be a nice clue of drug discovery or drug design for
the treatment of prion diseases.
