  The use of an extra SDS separation in a different buffer system provide a
technique for deconvoluting 2D gel spots made of several proteins (Colignon et
al. Proteomics, 2013, 13, 2077-2082). This technique keeps the quantitative
analysis of the protein amounts and combines it with a strongly improved
identification process by mass spectrometry, removing identification
ambiguities in most cases. In some favorable cases, posttranslational variants
can be separated by this procedure. This versatile and easy to use technique is
anticipated to be a very valuable addition to the toolbox used in 2D gel-based
proteomics.
