  Subtle changes in Raman spectral line-shape have been observed from malignant
human brain cells and its possibility for being used in detection and grading
of Glioma has been explored here. The latter has been developed as a result of
the fact that the width of the Raman spectra is more sensitive, as compared to
the peak position, to the brain tumors. The perturbations induced by the
cell-modification, as a consequence to the cancerous growth, may be responsible
for the widths variation in the Raman spectrum due to vibrational lifetime
alteration enforced at the molecular levels. A consistent cancer induced effect
on the spectral width has been observed for three different brain cells Raman
modes at different frequencies . Raman spectral analysis reveals that for
cancerous cells, the FWHM varies up to 35 % in comparison with the healthy
cells. It has been established how a careful analysis of Raman spectra can help
in easy detection of brain tumors. The methodology has been validated by
studying the effect of similar microscopic perturbations, e.g, Fano coupling
and quantum size effects, on different Raman spectral parameters which also
reveals Raman width to be the most sensitive parameter.
