  Mixing in an active micromixer was achieved using Lorentz force-assisted
actuation of an enameled copper wire. A single-step template-assisted soft
lithography technique was used to construct the mixing chamber. The chamber had
a volume of 1.86{\mu}l. Application of a Square wave alternating current in
tandem with tension in the wire provided the necessary conditions for the
resonant oscillation frequency of the wire. The repeatability of the ratio of
higher harmonics to the fundamental frequencies of the oscillating wire
conforms to standardization of the device fabrication, assembly, and
functionality. Simulations and experiments were performed to validate uniform
temperature distribution in the mixing chamber. Real-time optical detection of
the sample assisted in sensing the completion of chemical reactions in the
chamber. Mixing of various aqueous based chemical reactions was performed. It
was found that mixing efficiency was greater than 95 percent. Multiple devices
were fabricated to show the usability and reproducibility of the system.
