  Optofluidics is dedicated to achieving integrated control of particle and
fluid motion, particularly on the micrometer scale, by utilizing light to
direct fluid flow and particle motion. The field has seen significant growth
recently, driven by the concerted efforts of researchers across various
scientific disciplines, notably for its successful applications in biomedical
science. In this review, we explore a range of optofluidic architectures
developed over the past decade, with a primary focus on mechanisms for precise
control of micro and nanoscale biological objects and their applications in
sensing. Regarding nanoparticle manipulation, we delve into mechanisms based on
optical nanotweezers using nanolocalized light fields and light-based hybrid
effects with dramatically improved performance and capabilities. In the context
of sensing, we emphasize those works that used optofluidics to aggregate
molecules or particles to promote sensing and detection. Additionally, we
highlight emerging research directions, encompassing both fundamental
principles and practical applications in the field.
