  Brain modulation is a modification process of brain activity through external
stimulations. However, which condition can induce the activation is still
unclear. Therefore, we aimed to identify brain activation conditions using 40
Hz monaural beat (MB). Under this stimulation, auditory sense status which is
determined by frequency and power range is the condition to consider. Hence, we
designed five sessions to compare; no stimulation, audible (AB), inaudible in
frequency, inaudible in power, and inaudible in frequency and power. Ten
healthy participants underwent each stimulation session for ten minutes with
electroencephalogram (EEG) recording. For analysis, we calculated the power
spectral density (PSD) of EEG for each session and compared them in frequency,
time, and five brain regions. As a result, we observed the prominent power peak
at 40 Hz in only AB. The induced EEG amplitude increase started at one minute
and increased until the end of the session. These results of AB had significant
differences in frontal, central, temporal, parietal, and occipital regions
compared to other stimulations. From the statistical analysis, the PSD of the
right temporal region was significantly higher than the left. We figure out the
role that the auditory sense is important to lead brain activation. These
findings help to understand the neurophysiological principle and effects of
auditory stimulation.
