  In the present paper we deepen the works of L. Abatangelo, V. Felli, L.
Hillairet and C. Lena on the asymptotic estimates of the eigenvalue variation
under removal of segments from the domain in R2. We get a sharp asymptotic
estimate when the eigenvalue is simple and the removed segment is tangent to a
nodal line of the associated eigenfunction. Moreover, we extend their results
to the case when the eigenvalue is not simple.
