  In order to image live cells for prolonged periods of time, an Arduino-based,
low-cost imaging incubator was constructed. The imaging incubator keeps cells
viable by controlling for temperature and CO2 in order to maintain
physiological conditions for cells during imaging. All devices and parts
employed in the build were typical maker-type components in order to minimize
the cost of the imaging incubator. The imaging incubator allows for real-time
imaging of live cells exposed to any desired perturbation or stimulus. As a
proof of the system's functionality, cells are imaged over 24 hours while
remaining viable in the imaging incubator.
