  Purpose The aim of this study was to explore whether electroacupuncture
regulates phenotypic transformation of smooth muscle cells by inhibiting
ferroptosis and inhibiting fibrosis, thereby improving bladder urination
function after suprasacral spinal cord injury (SSCI). Methods The experiment
was divided into sham, model, and electroacupuncture group. After 10 days of
electroacupuncture intervention, urodynamic examination was performed, and
bladder neck was taken for HE staining, tandem mass tag (TMT)-based
quantitative proteomics analysis, Western blot(WB) detection, ferrous ion
concentration detection and Masson staining. Conclusion Electroacupuncture may
prevent the phenotype of bladder neck smooth muscle cells from transforming
from contraction type to synthesis type by inhibiting ferroptosis, inhibit
bladder neck fibrosis, improve bladder neck compliance, and thus improve
bladder urination function after SSCI.
