  In-house feeding system (IHF, a low dietary fiber source) may cause altered
cecal microbiota composition and inflammatory responses in meat geese via
increased endotoxemia (lipopolysaccharides) with reduced intestinal alkaline
phosphatase (ALP) production. The effects of artificial pasture grazing system
(AGF, a high dietary fiber source) on modulating gut microbiota architecture
and gut barrier functions have not been investigated in meat geese. The
intestinal ALP functions to regulate gut microbial homeostasis and barrier
function appears to inhibit pro-inflammatory cytokines by reducing LPS-induced
reactive oxygen species (ROS) production. The purpose of our study was to
investigate whether this enzyme could play a critical role in attenuating ROS
generation and then ROS facilitated NF-\k{appa}B pathway-induced systemic
inflammation in meat geese. First, we assessed the impacts of IHF and AGF on
gut microbial composition via 16 sRNA sequencing in meat geese. In the gut
microbiota analysis, meat geese supplemented with pasture demonstrated a
significant reduction in microbial richness and diversity compared to IHF meat
geese demonstrating antimicrobial, antioxidation, and anti-inflammatory ability
of AGF system. Second host markers analysis through protein expression of serum
and cecal tissues and quantitative PCR of cecal tissues were evaluated. We
confirmed a significant increase in intestinal ALP-induced Nrf2 signaling
pathway representing LPS dephosphorylation mediated TLR4/MyD88 induced ROS
reduction mechanisms in AGF meat geese. Further, the correlation analysis of
top 44 host markers with gut microbiota shows that artificial pasture intake
induced gut barrier functions via reducing ROS-mediated NF-\k{appa}B
pathway-induced gut permeability, systemic inflammation, and aging phenotypes.
