  Johnson-Kendall-Roberts (JKR) theory is an accurate model for strong adhesion
energies of soft slightly deformable material. Little is known about the
validity of this theory on complex systems such as living cells. We have
addressed this problem using a depletion controlled cell adhesion and measured
the force necessary to separate the cells with a micropipette technique. We
show that the cytoskeleton can provide the cells with a 3D structure that is
sufficiently elastic and has a sufficiently low deformability for JKR theory to
be valid. When the cytoskeleton is disrupted, JKR theory is no longer
applicable.
