  High-throughput cDNA sequencing (RNA-seq) is a very powerful technique to
quantify gene expression in an unbiased way. The Crustacean family is among the
groups of organisms sparsely represented in current genomic databases. Here we
present transcriptome data from Uca minax (red-jointed fiddler crab) as an
opportunity to extend our knowledge. Next generation sequencing was performed
on six tissue samples from Uca minax using the Illumina HiSeq system. Six
Transcriptome libraries were created using Trinity; a free, open-source
software tool for de novo transcriptome assembly of high-throughput mRNA
sequencing (RNA-seq) data with the absence of a reference genome. In addition,
several tools that aid in management of data were used, such as RSEM, Bowtie,
Blast, and IGV; a tool for visualizing RNA-seq analysis results. Fast quality
control (FastQC) analysis of the raw sequenced files revealed that both adapter
and PCR primer sequences were prevalently present, which may require a
preprocessing step.
