  The isochore concept in human genome sequence was challenged in an analysis
by the International Human Genome Sequencing Consortium (IHGSC). We argue here
that a statement in IGHSC analysis concerning the existence of isochore is
incorrect, because it had applied an inappropriate statistical test. To test
the existence of isochores should be equivalent to a test of homogeneity of
windowed GC%. The statistical test applied in the IHGSC's analysis, the
binomial test, is however a test of a sequence being random on the base level.
For testing the existence of isochore, or homogeneity in GC%, we propose to use
another statistical test: the analysis of variance (ANOVA). It can be shown
that DNA sequences that are rejected by binomial test may not be rejected by
the ANOVA test.
