  We study the effect of crowders on the melting profile of homogeneous and
heterogeneous DNA molecules. We find out the melting profile of short DNA
molecules and compare our findings with the experiments. We consider some
random distribution of crowders along the chain, and by finding out the best
match with the experiments, we attempt to identify the location of crowders in
the experimental findings of Ghosh \cite{Ghosh_PNAS_2020}. We also study the
melting of homogeneous DNA molecules of different lengths (25, 50, 75) in the
presence of only one crowder in the chain. By varying the location of the
crowder from one end to the other, we find that the melting temperature is
susceptible to the location of the crowder at the ends. At the same time, there
is minimal effect on the melting temperature due to the location of the
crowder. {\it In vivo}, the strength of a crowders may vary along the chain. We
study the melting of long heterogeneous chain in presence of five crowders of
different strength. We find that there is a significant variation in the
melting process of DNA in presence of crowders of variable strength.
