  The widespread application of nanomaterials in polymerase chain reaction
(PCR) technology has opened new avenues for improving detection methods in the
biomedical field. Recent experiments (Chem. Eur. J. 2023, e202203513) have
revealed oscillatory behavior between PCR efficiency and the concentration of
gold nanoparticles in the pM range, potentially linked to the long-range
Coulomb interactions among charged colloidal particles and the quantum size
effect of nanoparticle electronic states. Through Monte Carlo simulation, we
discovered that the radial distribution function of gold nanoparticles in
solution gradually exhibits peak characteristics with increasing charge,
triggering coherent photon behavior in Rayleigh scattering within the solution,
thereby influencing the efficiency of reusing released photons in the PCR chain
reaction. The study demonstrates that the oscillation period aligns with the
wavelength of downstream reaction photons, while their energy matches the width
of energy levels near the Fermi level of gold nanoparticles. The latter can
absorb and store electron states internally, promoting upstream PCR reactions
through subsequent re-release, and compensating for energy deficiencies through
the Boltzmann distribution of electrons. This work is poised to advance the
application of PCR-specific precise detection methods in the field of quantum
biotechnology.
