  Active diffusion of intracellular components is emerging as an important
process in cell biology. This process is mediated by complex assemblies of
molecular motors and cytoskeletal filaments that drive force generation in the
cytoplasm and facilitate enhanced motion. The kinetics of molecular motors have
been precisely characterized in-vitro by single molecule approaches, however,
their in-vivo behavior remains elusive. Here, we study the active diffusion of
vesicles in mouse oocytes, where this process plays a key role in nuclear
positioning during development, and combine an experimental and theoretical
framework to extract molecular-scale force kinetics (force, power-stroke, and
velocity) of the in-vivo active process. Assuming a single dominant process, we
find that the nonequilibrium activity induces rapid kicks of duration $\tau
\sim$ 300 $\mu$s resulting in an average force of $F \sim$ 0.4 pN on vesicles
in in-vivo oocytes, remarkably similar to the kinetics of in-vitro myosin-V.
Our results reveal that measuring in-vivo active fluctuations allows extraction
of the molecular-scale activity in agreement with single-molecule studies and
demonstrates a mesoscopic framework to access force kinetics.
