  Background: Pseudomonas aeruginosa has emerged as a significant opportunistic
bacterial pathogen that causes nosocomial infections in healthcare settings
resulting in treatment failure throughout the world. This study was carried out
to compare the relatedness between virulence characteristics and
\b{eta}-lactamase encoding genes producing Pseudomonas aeruginosa. Methods: A
total of 120 P. aeruginosa isolates were obtained from both paediatric and
adult patients of Selayang Hospital, Kuala Lumpur, Malaysia. Phenotypic methods
were used to detect various virulence factors (Phospholipase, Hemolysin,
Gelatinase, DNAse, and Biofilm). All the isolates were evaluated for production
of extended spectrum beta-lactamase (ESBL) as well as metallo \b{eta}-lactamase
(MBL) by Double-disk synergy test (DDST) and E-test while AmpC
\b{eta}-lactamase production was detected by disk antagonism test. Results: In
this study, 120 Pseudomonas aeruginosa isolates (20 each from blood, wounds,
respiratory secretions, stools, urine, and sputum samples) were studied. Among
Pseudomonas aeruginosa isolates, the distribution of virulence factors was
positive for hemolysin (48.33%), DNAse (43.33%), phospholipase (40.83%),
gelatinase (31.66%) production and biofilm formation (34%) respectively. The
prevalence of multiple \b{eta}-lactamase in P. aeruginosa showed 19.16% ESBL,
7.5% MBL and 10.83% AmpC production respectively. Conclusion: A regular
surveillance is required to reduce public healt
