  The detection of biomolecules at the single molecular level have important
applications in the fields of biosensing and biomedical diagnosis. Solid state
nanopore (SS-nanopore) is an effective tool to perform the single molecular
detection, due to its unique properties of label-free and less sample
consumption. The current SS-nanopore translocations of small biomolecules are
driven by the electronic field force, thus easily influenced by the charges,
shapes and sizes of the target molecules. Therefore, it remains a great
challenge to control biomolecules to translocate through SS-nanopore,
particularly for the protein with complex conformations and different charges.
Towards this problem, we developed a DNA scaffold carrier assisted strategy to
help protein translocation through SS-nanopore, thus facilitating the target
protein detection. Taking advantages of a DNA carrier loading effect, the
current signal to noise ratios are significantly improved. Our proposed method
is sensitive, convenient and can detect real-time sample nanopore
translocations. This method opens up a wide range of applications for
SS-nanopore detection and promote the development of single molecular
detections.
