  Understanding the structure of supramolecular complexes provides insight into
their functional capabilities and how they can be modulated in the context of
disease. Super-resolution microscopy (SRM) excels in performing this task by
resolving ultrastructural details at the nanoscale with molecular specificity.
However, technical limitations, such as underlabelling, preclude its ability to
provide complete structures. Single-particle analysis (SPA) overcomes this
limitation by combining information from multiple images of identical
structures and producing an averaged model, effectively enhancing the
resolution and coverage of image reconstructions. This review highlights
important studies using SRM-SPA, demonstrating how it broadens our knowledge by
elucidating features of key biological structures with unprecedented detail.
