  We report the self-assembly of thin actin shells beneath the membranes of
giant vesicles. Ion-carrier mediated influx of Mg2+ induces actin
polymerization in the initially spherical vesicles. Buckling of the vesicles
and the formation of blisters after thermally induced bilayer expansion is
demonstrated. Bilayer flickering is dominated by tension generated by its
coupling to the actin cortex. Quantitative flicker analysis suggests the
bilayer and the actin cortex are separated by 0.4 \mum to 0.5 \mum due to
undulation forces.
