  Osseointegration is one of the key conditions for long term successful dental
implantation. To this end, titanium alloys undergo plethora of surface
treatments able to sustain osteogenic differentiation. For these surface
treatments, femtosecond laser (FSL) can generate precise and reproducible
surface patterns on titanium, avoiding thermal damage and chemical pollution.
We recently identify that laser-induced periodic surface structure (LIPSS) with
radial orientation, generated on model (flat) titanium surface, has a high
osteogenic potential. However, nano-texturing is time consuming. In the present
study, we aimed to reduce the texturing time of radial LIPSS, as well as
processing of large commercially available dental screws by ways of laser beam
engineering. Our objectives were to maintain at least osteogenic properties
demonstrated on model surfaces by adjusting laser beam diameters and to
demonstrate maintenance of performance with a dental screw texturing process
not exceeding 1 minute.We first textured model surfaces with radial LIPSS by
laser beams of different diameters, with surface impacts of 24$\mu$m, 80$\mu$m
or 180$\mu$m, named as R24, R80 or R180 respectively. Osteogenic performance of
human mesenchymal stem cells (hMSCs) were compared; seeded on polished control
surfaces and textured surfaces and subjected to osteogenic evaluation by
cell/matrix imaging, qRT-PCR and mineral deposition quantification. All
textured surfaces showed greater osteogenic potential than the control
surfaces, with significantly higher efficacy on R180 surface. Therefore, R180
pattern with large beam impacts was chosen for texturing on a dental screw and
its osteogenic activity was compared to that of a non-textured screw.
Interestingly, R180 required only 40 seconds to be textured on whole screws, on
which it preserved a high osteogenic potential. Thus, by using FSL technology,
we have improved the osteogenic potential of a topographic pattern while
optimizing and scaling up its processing time on a medical device.
