  Single molecule detection has revolutionised the fields of chemistry and
biology by offering powerful ways to study individual molecules under different
scenarios. Nanophotonic structures, including plasmonic antennas, significantly
overcome the concentration limit at which single molecule events can be
observed, enabling their detection at concentrations that are relevant to
biological and chemical processes. Although antennas can be fabricated in large
arrays, probing dynamic events requires high temporal resolution, which is best
achieved by serial antenna interrogation. Unfortunately, this precludes the
simultaneous recording from multiple antennas at different sample locations,
and is time consuming, resulting in poor statistics and low-throughput data
acquisition, abating the true potential of arrays. Here we exploit arrays of
antenna-in-box nanostructures in combination with sCMOS readout to interrogate
nanoscale volumes from 225 antennas simultaneously. Recording at 1 kHz allowed
multiplexed dynamic measurements from 50 nanoantennas simultaneously with a
temporal resolution dictated by the camera framerate and the photons emitted
per molecule during a single passage. We demonstrate the capability for
high-throughput arrayed detection of single molecule dynamics at the nanoscale
in the membrane of living cells, and determine the axial location of membrane
molecular components with 1 nm accuracy and temporal resolution below 1 ms.
