  Label-free combined CARS and SHG microscopy techniques are used as powerful
tool to follow the cells behavior in cell-scaffold construct for regeneration
of tissues. Imaging of histological section of hDFs seeded in fibrin gel
scaffold and imaging of collagen produced by hDFs in a time course experiment
at different culture days (0, 7, 21, 42) is performed. A study on the limit of
collagen detection of the imaging system is reported using sample prepared with
different collagen concentrations. The results show that also the small amount
of collagen produced by hDFs after few hours of incubation in fibrin gel is
detected. Co-localization of hDFs and collagen is also reported in function of
the culture days.
