  We report on determinations of the low-energy constants alpha5 and alpha8 in
the effective chiral Lagrangian at O(p^4), using lattice simulations with N_f=2
flavours of dynamical quarks. Precise knowledge of these constants is required
to test the hypothesis whether or not the up-quark is massless. Our results are
obtained by studying the quark mass dependence of suitably defined ratios of
pseudoscalar meson masses and matrix elements. Although comparisons with an
earlier study in the quenched approximation reveal small qualitative
differences in the quark mass behaviour, numerical estimates for alpha5 and
alpha8 show only a weak dependence on the number of dynamical quark flavours.
Our results disfavour the possibility of a massless up-quark, provided that the
quark mass dependence in the physical three-flavour case is not fundamentally
different from the two-flavour case studied here.
