  The poor contrast and the overlapping of cervical cell cytoplasm are the
major issues in the accurate segmentation of cervical cell cytoplasm. This
paper presents an automated unsupervised cytoplasm segmentation approach which
can effectively find the cytoplasm boundaries in overlapping cells. The
proposed approach first segments the cell clumps from the cervical smear image
and detects the nuclei in each cell clump. A modified Otsu method with prior
class probability is proposed for accurate segmentation of nuclei from the cell
clumps. Using distance regularized level set evolution, the contour around each
nucleus is evolved until it reaches the cytoplasm boundaries. Promising results
were obtained by experimenting on ISBI 2015 challenge dataset.
