  Methods for evaluating the quality of genomic and metagenomic data are
essential to aid genome assembly and to correctly interpret the results of
subsequent analyses. BUSCO estimates the completeness and redundancy of
processed genomic data based on universal single-copy orthologs. Here we
present new functionalities and major improvements of the BUSCO software, as
well as the renewal and expansion of the underlying datasets in sync with the
OrthoDB v10 release. Among the major novelties, BUSCO now enables phylogenetic
placement of the input sequence to automatically select the most appropriate
dataset for the assessment, allowing the analysis of metagenome-assembled
genomes of unknown origin. A newly-introduced genome workflow increases the
efficiency and runtimes especially on large eukaryotic genomes. BUSCO is the
only tool capable of assessing both eukaryotic and prokaryotic species, and can
be applied to various data types, from genome assemblies and metagenomic bins,
to transcriptomes and gene sets.
