  Understanding neuronal communication is fundamental in neuroscience but there
are few methodologies offering detailed analysis for well-controlled
conditions. By interfacing microElectrode arrays with microFluidics ({\mu}EF
devices), it is possible to compartmentalize neuronal cultures with a specified
alignment of axons and microelectrodes. This setup allows extracellular
recordings of spike propagation with high signal-to-noise ratio over the course
of several weeks. Addressing these {\mu}EF systems we developed an advanced,
yet easy-to-use, open-source computational tool, {\mu}SpikeHunter, which
provides detailed quantification of several communication-related properties
such as propagation velocity, conduction failure, spike timings, and coding
mechanisms. The combination of {\mu}EF devices and {\mu}SpikeHunter can be used
in the context of standard neuronal cultures or with co-culture configurations
where, for example, communication between sensory neurons and other cell types
is monitored and assessed. The ability to analyze axonal signals (in a
user-friendly, time-efficient, high-throughput manner) opens doors to new
approaches to studies of peripheral innervation, neural coding, and
neuroregeneration approaches, among many others. We demonstrate the use of
{\mu}SpikeHunter in dorsal root ganglion neurons where we analyze the presence
of anterograde signals in {\mu}EF devices.
