  Objective: The study explored therapeutic targets and mechanisms of Gegen
Qinlian Decoction for Helicobacter pylori infection and related gastric cancer
using network pharmacology, molecular docking, and Mendelian randomization.
  Methods: Medicinal components of Gegen Qinlian Decoction were extracted from
TCMSP and HERB databases. Disease treatment targets were sourced from DisGeNET
and PubChem. Interaction networks were constructed via the STRING database and
visualized using Cytoscape 3.9.1. Enrichment analysis of intersected targets
was performed using DAVID and Metascapes. Molecular docking employed Autodock
Tools 1.5.6 and PyMOL 2.5.2. Mendelian randomization was based on the ukb-b-531
sample from UK Biobank.
  Results: 146 active components and 248 targets from Gegen Qinlian Decoction
were identified. 66 targets overlapped with Helicobacter pylori infection
genes. Molecular docking highlighted interactions between primary drug
components like quercetin, wogonin, kaempferol, and target genes PTGS1, PTGS2,
MAPK14. Mendelian randomization pinpointed genes like IGF2, PIK3CG, GJA1, and
PLAU associated with Helicobacter pylori infection.
  Conclusion: Gegen Qinlian Decoction's active components target Helicobacter
pylori infection through diverse targets and pathways, presenting potential
research avenues.
