  Protein splicing is a post-translational autocatalystic excision of internal
protein sequence (intein) with the subsequent ligation of the flanking
polypeptides (exteins). The high specificity of excision ensured by intein
makes it possible to use a phenomenon of protein splicing for the biotechnology
purposes. The aim of this work was optimization of obtaining and purification
of the recombinant human growth hormone using the protein splicing. It was
experimentally demonstrated that the use of modified intein as auto-removal
affine marker makes it possible to perform the rapid and cheap isolation of the
recombinant protein Hgh. Furthermore, this approach allows to obtain the human
growth hormone with native N-terminus, without formyl-metionine. Key words:
intein, human growth hormone, protein splicing
