  Recent efforts to survey the numerous softwares available to perform single
molecule tracking (SMT) highlighted a significant dependence of the outcomes on
the specific method used, and the limitation encountered by most techniques to
capture fast movements in a crowded environment. Other approaches to identify
the mode and rapidity of motion of fluorescently labeled biomolecules, that do
not relay on the localization and linking of the images of isolated single
molecules are, however, available.This direct comparison shows that correlative
imaging analysis approaches complement effectively current SMT methods in
circumstances when, due to either the density of the sample, the low signal to
noise ratio or molecular blinking, trajectory linking does not allow to capture
long-range or fast motion.
