  We demonstrate an electro-optic wide-field method to enable fluorescence
lifetime microscopy (FLIM) with high throughput and single-molecule
sensitivity. Resonantly driven Pockels cells are used to efficiently gate
images at 39 MHz, allowing fluorescence lifetime to be captured on standard
camera sensors. Lifetime imaging of single molecules is enabled in wide-field
with exposure times of less than 100 milliseconds. This capability allows
combination of wide-field FLIM with single-molecule super-resolution
localization microscopy. Fast single-molecule dynamics such as FRET and
molecular binding events are captured from wide-field images without prior
spatial knowledge. A lifetime sensitivity of 1.9 times the photon shot-noise
limit is achieved, and high throughput is shown by acquiring wide-field FLIM
images with millisecond exposure and $>10^8$ photons per frame. Resonant
electro-optic FLIM allows lifetime contrast in any wide-field microscopy
method.
