  The formation of a collectively moving group benefits individuals within a
population in a variety of ways such as ultra-sensitivity to perturbation,
collective modes of feeding, and protection from environmental stress. While
some collective groups use a single organizing principle, others can
dynamically shift the behavior of the group by modifying the interaction rules
at the individual level. The surface-dwelling bacterium Myxococcus xanthus
forms dynamic collective groups both to feed on prey and to aggregate during
times of starvation. The latter behavior, termed fruiting-body formation,
involves a complex, coordinated series of density changes that ultimately lead
to three-dimensional aggregates comprising hundreds of thousands of cells and
spores. This multi-step developmental process most likely involves several
different single-celled behaviors as the population condenses from a loose,
two-dimensional sheet to a three-dimensional mound. Here, we use
high-resolution microscopy and computer vision software to spatiotemporally
track the motion of thousands of individuals during the initial stages of
fruiting body formation. We find that a combination of cell-contact-mediated
alignment and internal timing mechanisms drive a phase transition from
exploratory flocking, in which cell groups move rapidly and coherently over
long distances, to a reversal-mediated localization into streams, which act as
slow-spreading, quasi-one-dimensional nematic fluids. These observations lead
us to an active liquid crystal description of the myxobacterial development
cycle.
