  Understanding the coupling of graphene with its local environment is critical
to be able to integrate it in tomorrow's electronic devices. Here we show how
the presence of a metallic substrate affects the properties of an atomically
tailored graphene layer. We have deliberately introduced single carbon
vacancies on a graphene monolayer grown on a Pt(111) surface and investigated
its impact in the electronic, structural and magnetic properties of the
graphene layer. Our low temperature scanning tunneling microscopy studies,
complemented by density functional theory, show the existence of a broad
electronic resonance above the Fermi energy associated with the vacancies.
Vacancy sites become reactive leading to an increase of the coupling between
the graphene layer and the metal substrate at these points; this gives rise to
a rapid decay of the localized state and the quenching of the magnetic moment
associated with carbon vacancies in free-standing graphene layers.
