  Lipid droplets (LDs), once considered mere storage depots for lipids, have
gained recognition for their intricate roles in cellular processes, including
metabolism, membrane trafficking, and disease states like obesity and cancer.
This review explores label-free imaging techniques' applications in LD
research. We discuss holotomography and vibrational spectroscopic microscopy,
emphasizing their potential for studying LDs without molecular labels, and we
highlight the growing integration of artificial intelligence. Clinical
applications in disease diagnosis and therapy are also considered.
