  AlphaFold2 (AF2) has emerged in recent years as a groundbreaking innovation
that has revolutionized several scientific fields, in particular structural
biology, drug design and the elucidation of disease mechanisms. Many scientists
now use AF2 on a daily basis, including non-specialist users. This chapter is
aimed at the latter. Tips and tricks for getting the most out of AF2 to produce
a high quality biological model are discussed here. We suggest to
non-specialist users how to maintain a critical perspective when working with
AF2 models and provide guidelines on how to properly evaluate them. After
showing how to perform our own structure prediction using ColabFold, we list
several ways to improve AF2 models by adding information that is missing from
the original AF2 model. By using software such as AlphaFill to add cofactors
and ligands to the models, or MODELLER to add disulfide bridges between
cysteines, we guide users to build a high quality biological model suitable for
applications such as drug design, protein interaction or molecular dynamics
studies.
