  The nonselective cation channel TRPC4 has been shown to be present in high
abundance in the corticolimbic regions of the brain and play a pivotal role in
modulating cellular excitability due to their involvement in intracellular Ca2+
regulation. Recently we reported their involvement in socialization and
regulating anxiety-like behaviors in rats. Given the important role for
dopamine in modulating emotions involved in social anxiety we investigated
whether TRPC4 protein and mRNA was found on dopaminergic neurons of the ventral
tegmental area (VTA). Using emulsion autoradiography we found that TRPC4 mRNA
is indeed present in the VTA and the substantia nigra. Additionally,
immunohistochemistry verified it's presence on a subpopulation of dopamine
neurons in the VTA. We confirmed these findings by testing Trpc4 knock-out rats
in addition to wild-type animals. This novel finding suggests that TRPC4 plays
a pivotal role in regulating dopamine release in a sub-population of neurons
that may modulate emotional and cognitive responses in social situations.
