  Fluorescence microscopy images usually show severe anisotropy in axial versus
lateral resolution. This hampers downstream processing, i.e. the automatic
extraction of quantitative biological data. While deconvolution methods and
other techniques to address this problem exist, they are either time consuming
to apply or limited in their ability to remove anisotropy. We propose a method
to recover isotropic resolution from readily acquired anisotropic data. We
achieve this using a convolutional neural network that is trained end-to-end
from the same anisotropic body of data we later apply the network to. The
network effectively learns to restore the full isotropic resolution by
restoring the image under a trained, sample specific image prior. We apply our
method to $3$ synthetic and $3$ real datasets and show that our results improve
on results from deconvolution and state-of-the-art super-resolution techniques.
Finally, we demonstrate that a standard 3D segmentation pipeline performs on
the output of our network with comparable accuracy as on the full isotropic
data.
