  Point-scanning microscopy approaches are transforming super-resolution
imaging. Despite achieving parallel high-speed imaging using multifocal
techniques, efficient multi-color capability with high-quality illumination is
currently lacking. In this paper, we present for the first time
Mechanical-scan-free and multi-Color Super-resolution Microscopy (MCoSM) by
spot array illumination, which enables mechanical-scan-free super-resolution
imaging with adjustable resolution and field of view (FoV) based on spatial
light modulators (SLMs). Through 100s-10,000s super-resolution spot
illumination with different FoV for imaging, we demonstrate the adjustable
capacity of MCoSM. MCoSM extends current spectral imaging capabilities through
a time-sharing process of different color illumination with phase-shifting
scanning, while retaining the spatial flexibility of super-resolution imaging
with diffractive spot array illumination. To showcase the prospects for further
combining MCoSM with multi-color imaging, we also perform spectral unmixing
(four-colors) on images of fluorescent beads at high resolution. MCoSM provides
a versatile platform for studying molecular interactions in complex samples at
the nanoscale level.
