  We study the twisted N=4 super Yang-Mills theories in the Omega-background
with the constant R-symmetry Wilson line gauge field. Based on the
classification of topological twists of N=4 supersymmetry (the half, the
Vafa-Witten and the Marcus twists), we construct the deformed off-shell
supersymmetry associated with the scalar supercharges for these twists. We find
that the Omega-deformed action is written in the exact form with respect to the
scalar supercharges as in the undeformed case.
