  Sample-induced image-degradation remains an intricate wave-optical problem in
light-sheet microscopy. Here we present biobeam, an open-source software
package that enables to simulate operational light-sheet microscopes by
combining data from 10^5 - 10^6 multiplexed and GPU-accelerated
point-spread-function calculations. The wave-optical nature of these
simulations leads to the faithful reproduction of spatially varying
aberrations, diffraction artifacts, geometric image distortions, adaptive
optics, and emergent wave optical phenomena, and renders image-formation in
light-sheet microscopy computationally tractable.
