  Point spread function (PSF) plays an essential role in image reconstruction.
In the context of confocal microscopy, optical performance degrades towards the
edge of the field of view as astigmatism, coma and vignetting. Thus, one should
expect the related artifacts to be even stronger in macroscopy, where the field
of view is much larger. The field aberrations in macroscopy fluorescence
imaging system was observed to be symmetrical and to increase with the distance
from the center of the field of view. In this paper we propose an experiment
and an optimization method for assessing the center of the field of view. The
obtained results constitute a step towards reducing the number of parameters in
macroscopy PSF model.
