  Presently 4T-1 luc cells were irradiated with proton under ultra-high dose
rate FLASH or with gamma-ray with conventional dose rate, and then subcutaneous
vaccination with or without Mn immuno-enhancing adjuvant into the mice for
three times. One week later, we injected untreated 4T-1 luc cells on the other
side of the vaccinated mice, and found that the untreated 4T-1 luc cells
injected later nearly totally did not grow tumor (1/17) while controls without
previous vaccination all grow tumors (18/18). The result is very interesting
and the findings may help to explore in situ tumor vaccination as well as new
combined radiotherapy strategies to effectively ablate primary and disseminated
tumors. To our limited knowledge, this is the first paper reporting the high
efficiency induction of systemic vaccination suppressing the
metastasized/disseminated tumor progression.
