  We report on changes in the Raman spectrum of live Escherichi coli (E.coli)
that result from exposure to lethal fluences of 300 nm (UV-B) and 405 nm
(violet) photons. In the first instance, the energy per photon of 4.13 eV is
sufficient to break most of the inter-atomic bonds in the bacterium and major
change in the Raman spectrum, particularly in the RNA/DNA regions is observed.
This energy is in near resonance with the C-H, N-H, and P-O bond binding
energies that interconnect phosphate backbone sections and may initiate the
Raman modifications. By contrast, the 3.06 eV violet photon energy is
insufficient to cleave the stronger system bonds. The much larger lethal
fluence required in this case produces a significant change in the resonant C-N
and C-P bond connected amino acid/protein groups and lipid peak signal but much
less so in the nucleotide-DNA/RNA signature.
