  Electrically powered micro- and nanomotors are promising tools for in-vitro
single-cell analysis. In particular, single cells can be trapped, transported
and electroporated by a Janus particle (JP) using an externally applied
electric field. However, while dielectrophoretic (DEP)-based cargo manipulation
can be achieved at high-solution conductivity, electrical propulsion of these
micromotors becomes ineffective at solution conductivities exceeding 0.3mS/cm.
Here, we successfully extended JP cargo manipulation and transport capabilities
to conductive near-physiological (<6mS/cm) solutions by combining magnetic
field-based micromotor propulsion and navigation with DEP-based manipulation of
various synthetic and biological cargos. Combination of a rotating magnetic
field and electric field resulted in enhanced micromotor mobility and steering
control through tuning of the electric field frequency. conditions are
necessary. In addition, we demonstrated the micromotors ability of identifying
apoptotic cell among viable and necrotic cells based their dielectrophoretic
difference, thus, enabling to analyze the apoptotic status in the single cell
samples for drug discovery, cell therapeutics and immunotherapy. We also
demonstrated the ability to trap and transport live cells towards regions
containing doxorubicin-loaded liposomes. This hybrid micromotor approach for
label-free trapping, transporting and sensing of selected cells within
conductive solutions, opens new opportunities in drug delivery and single cell
analysis, where close-to-physiological media
