  We study the conformations of a semiflexible chain, confined in nano-scaled
spherical cavities, under two distinct processes of confinement. Radial
contraction and packaging are employed as two confining procedures. The former
method is performed by gradually decreasing the diameter of a spherical shell
which envelopes a confined chain. The latter procedure is carried out by
injecting the chain inside a spherical shell through a hole on the shell
surface. The chain is modeled with a rigid body molecular dynamics simulation
and its parameters are adjusted to DNA base-pair elasticity. Directional order
parameter is employed to analyze and compare the confined chain and the
conformations of the chain for two different sizes of the spheres are studied
in both procedures. It is shown that for the confined chains in the sphere
sizes of our study, they appear in spiral or tennis-ball structures, and the
tennis-ball structure is more likely to be observed in more compact
confinements. Our results also show that the dynamical procedure of confinement
and the rate of the confinement are influential parameters of the structure of
the chain inside spherical cavities.
