  The technique presented here identifies tethered mould designs, optimised for
growing cultured tissue with very highly-aligned cells. It is based on a
microscopic biophysical model for polarised cellular hydrogels. There is an
unmet need for tools to assist mould and scaffold designs for the growth of
cultured tissues with bespoke cell organisations, that can be used in
applications such as regenerative medicine, drug screening and cultured meat.
High-throughput biophysical calculations were made for a wide variety of
computer-generated moulds, with cell-matrix interactions and tissue-scale
forces simulated using a contractile-network dipole-orientation model.
Elongated moulds with central broadening and one of the following tethering
strategies are found to lead to highly-aligned cells: (1) tethers placed within
the bilateral protrusions resulting from an indentation on the short edge, to
guide alignment (2) tethers placed within a single vertex to shrink the
available space for misalignment. As such, proof-of-concept has been shown for
mould and tethered scaffold design based on a recently developed biophysical
model. The approach is applicable to a broad range of cell types that align in
tissues and is extensible for 3D scaffolds.
