  The proton-magnetic reaction is commonly used in MRI machines with a strong
magnetic field of over 1 T, while this study hypothesized that the electron
magnetic reaction of hydrogen could affect the hydrogen bonds of
double-stranded DNA (dsDNA) at a low magnetic field below 0.01 T. The goal is
to develop a hydrogen bonding magnetic reaction-based gene regulation (HBMR-GR)
system. The polarities of DNA base pairs are derived from the relative
electrostatic charge between purines and pyrimidines, which become positively
and negatively charged, respectively. The Pyu dsDNAs with
pyrimidine(s)-purine(s) sequences, ds3T3A, ds3C3G, and ds3C3A, showed stronger
DNA hybridization potential, increased infrared absorption at 3400-3200 cm-1,
and a unique DNA conformation in HPLC analysis compared to the corresponding
Puy dsDNAs. To target the three-dimensional structure of dsDNA based on the DNA
base pair polarities, one can use cyclic electromagnetic DNA simulation (CEDS)
with approximately 25% efficiency for randomly oriented dsDNAs. CEDS was found
to induce sequence-specific hybridization of target oligo-dsDNAs in 0.005M NaCl
solution and sequence-specific conformation of oligo-dsDNAs in 0.1M NaCl
solution. It was found that the Pyu oligo-dsDNAs were more responsible for the
hybridization and conformational changes by CEDS than the Puy oligo-dsDNAs.
CEDS decreased ethidium bromide (EtBr) DNA intercalation and spermidine DNA
condensation depending on CEDS time in the binding assay. The results also
included that the Pyu oligo-dsDNAs were more responsible for CEDS by forming
stable and unique conformation of oligo-dsDNA than the Therefore, it is
postulated that the low-level HBMR-based CEDS can enhance the hybridization
potential of oligo-dsDNAs and subsequently lead to the unique DNA conformation
required for the initiation of various DNA functions.
