  Development of reliable and eco-friendly processes for synthesis of metallic
nanoparticles is an important step in the field of application of
nanotechnology. In the present study, extracellular synthesis of silver
nanoparticles using culture supernatant of plant growth promoting
rhizobacterium, Pseudomonas sp.ARS22. The biosynthesis of AgNPs by Pseudomonas
sp.ARS22 was monitored by UVvisible spectrum that showed the surface plasmon
resonance peak at 410 nm, an important characteristic of AgNPs. Furthermore,
the morphological, elemental, functional and thermal characterization of
arsAgNPs was carried out using the electron and atomic microscopies, FTIR
spectroscopy and Atomic Force microscopy, respectively. The arsAgNPs were
spherical in shape. The DebyeScherrer equation was used to calculate particle
sizes and the silver nanoparticles of approximate size 10 to 30 nm were
observed. The process of reduction is extracellular, which makes it an easier
method for the synthesis of silver nanoparticles.
