  Reliability of kinetic parameters are crucial in understanding enzyme
kinetics within cellular system. The present study suggests a few cautions that
need introspection for estimation of parameters like K(M), V(max) and K(I)
using Lineweaver-Burk plots. The quality of IC(50) too needs a thorough
reinvestigation because of its direct link with K(I) and K(M) values.
Inhibition kinetics under both steady-state and non-steady-state conditions are
studied and errors in estimated parameters are compared against actual values
to settle the question of their adequacy.
