  With the acceleration of urbanization and living standards, microorganisms
play increasingly important roles in industrial production, bio-technique, and
food safety testing. Microorganism biovolume measurements are one of the
essential parts of microbial analysis. However, traditional manual measurement
methods are time-consuming and challenging to measure the characteristics
precisely. With the development of digital image processing techniques, the
characteristics of the microbial population can be detected and quantified. The
changing trend can be adjusted in time and provided a basis for the
improvement. The applications of the microorganism biovolume measurement method
have developed since the 1980s. More than 62 articles are reviewed in this
study, and the articles are grouped by digital image segmentation methods with
periods. This study has high research significance and application value, which
can be referred to microbial researchers to have a comprehensive understanding
of microorganism biovolume measurements using digital image analysis methods
and potential applications.
