  Surface Enhanced Laser Desorption/Ionization-Time Of Flight Mass Spectrometry
(SELDI-TOF MS) is a variant of the MALDI. It is uses in many cases especially
for the analysis of protein profiling and for preliminary screening tasks of
complex sample aimed for the searching of biomarker. Unfortunately, these
analysis are time consuming and strictly limited about the protein
identification. Seldi analysis of mass spectra (SELYMATRA) is a Web Application
(WA) developed with the aim of reduce these lacks automating the identification
processes and introducing the possibility to predict the proteins present in
complex mixtures from cells and tissues analysed by Mass Spectrometry.
SELYMATRA has the following characteristics. The architectural pattern used to
develop the WA is the Model-View-Controller (MVC), extremely used in the
development of software system. The WA expects an user to upload data in a
Microsoft Excel spreadsheet file format, usually generated by means of the
proprietary Mass Spectrometry softwares. Several parameters can be set such as
experiment conditions, range of isoelectric point, range of pH, relative errors
and so on. The WA compare the mass value among two mass spectra (sample vs
control) to extract differences, and according to the parameters set, it
queries a local database for the prediction of the most likely proteins related
to the masses differently expressed. The WA was validated in a cellular model
overexpressing a tagged NURR1 receptor. SELYMATRA is available at
http://140.164.61.23:8080/SELYMATRA.
