  Optical sectioning technology has been widely used in various fluorescence
microscopes owing to its background removing capability. Here, a virtual HiLo
based on edge detection (V-HiLo-ED) is proposed to achieve wide-field optical
sectioning, which requires only single wide-field image. Compared with
conventional optical sectioning technologies, its imaging speed can be
increased by at least twice, meanwhile maintaining nice optical sectioning
performance, low cost, and excellent artifact suppression capabilities.
Furthermore, the new V-HiLo-ED can also be extended to other non-fluorescence
imaging fields. This simple, cost-effective and easy-to-extend method will
benefit many research and application fields that needs to remove out-of-focus
blurred images.
