  The rapid development of the DNA nanotechnology field has been facilitated by
advances in CAD software. However, as more complex concepts arose, the lag
between the needs and software capabilities appeared. Further derailed by
manual installation and software incompatibility across different platforms and
often tedious library management issues, the software has become hard-to-use
for many.
  Here we present NanoFrame, a web-based DNA wireframe design tool for making
3D nanostructures from a single scaffold. Within this software, we devised
algorithms for DNA routing, staple breaking, and wireframe cage opening which,
while modeled for cuboid structure, can be generalized to a variety of platonic
and Archimedean shapes. In addition, NanoFrame provides a platform for editing
auto-generated staple sequences and saving work online.
